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Expression of a High Mobility group Protein Isolated from Cucumis sativus Affects the Germination of Arabidopsis thaliana under Abiotic Stress Conditions

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Journal of Integrative Plant Biology 2008年第5期50卷 593-600页

作者： Ji Young Jang Kyung Jin Kwak Hunseung Kang Department of Plant biotechnology Agricultural Plant Stress Research Center and Biotechnology Research InstituteCollege of Agriculture and Life SciencesChonnam National UniversityGwangju 500-757Korea

Although high mobility group B （HMGB） proteins have been identified from a variety of plant species, their importance and functional roles in plant responses to changing environmental conditions are largely unknown. Here, we investigated the functional roles of a CsHMGB isolated from cucumber （Cucumis sativus L.） in plant responses to environmental stimuli. Under normal growth conditions or when subjected to cold stress, no differences in plant growth were found between the wild-type and transgenic Arabidopsis thaliana overexpressing CsHMGB. By contrast, the transgenic Arabidopsis plants displayed retarded germination compared with the wild-type plants when grown under high salt or dehydration stress conditions. Germination of the transgenic plants was delayed by the addition of abscisic acid （ABA）, implying that CsHMGB affects germination through an ABA-dependent way. The expression of CsHMGB had affected only the germination stage, and CsHMGB did not affect the seedling growth of the transgenic plants under the stress conditions. The transcript levels of several germination-responsive genes were modulated by the expression of CsHMGB in Arabidopsis. Taken together, these results suggest that ectopic expression of a CsHMGB in Arabidopsis modulates the expression of several germinationresponsive genes, and thereby affects the germination of Arabidopsis plants under different stress conditions.

关键词： abiotic stress cucumber Cucumis sativus high mobility group protein transgenic Arabidopsis plant.

Regulation of intestinal stem cell fate specification

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Science China(life sciences) 2015年第6期58卷 570-578页

作者： QI Zhen CHEN Ye-Guang State Key Laboratory of Biomembrane and Membrane biotechnology Tsinghua-Peking Center for Life Sciences School of Life SciencesTsinghua University

The remarkable ability of rapid self-renewal makes the intestinal epithelium an ideal model for the study of adult stem cells. The intestinal epithelium is organized into villus and crypt, and a group of intestinal stem cells located at the base of crypt are responsible for this constant self-renewal throughout the life. Identification of the intestinal stem cell marker Lgr5, isolation and in vitro culture of Lgr5+ intestinal stem cells and the use of transgenic mouse models have significantly facilitated the studies of intestinal stem cell homeostasis and differentiation, therefore greatly expanding our knowledge of the regulatory mechanisms underlying the intestinal stem cell fate determination. In this review, we summarize the current understanding of how signals of Wnt, BMP, Notch and EGF in the stem cell niche modulate the intestinal stem cell fate.

关键词： intestinal stem cells Wnt BMP Notch EGF fate specification

Beneficial effects of AAV1-Rheb(S16H) administration in the adult hippocampus

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Neural Regeneration research 2020年第8期15卷 1479-1480页

作者： Sang Ryong Kim School of life sciences BK21 plus KNU Creative Bioresearch group Institute of life Science & biotechnology Brain Science and Engineering Institute Kyungpook National University

Neurotrophic factors against neurodegenerative diseases such as Alzheimer’s disease(AD)and Parkinson’s disease(PD):A clear understanding of the etiology of neurodegenerative diseases,such as AD and PD remains *** there is still no therapy to prevent or block neurodegeneration,in vivo and in vitro experimental results have shown that the direct administration of neurotrophic factors,such as brain-derived neurotrophic factor(BDNF)and ciliary neurotrophic factor(CNTF),and the induction of these neurotrophic factors via specific gene delivery may protect neurons against neurotoxicity in neurodegenerative disease models(Kim et al.,2012;Jeong et al.,2013,2015).

关键词： al. diseases Alzheimer

Protecting future antimalarials from the trap of resistance:Lessons from artemisinin-based combination therapy (ACT) failures

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Journal of Pharmaceutical Analysis 2021年第5期11卷 541-554页

作者： Nekpen Erhunse Dinkar Sahal Malaria Drug Discovery research group International Centre for Genetic Engineering and BiotechnologyNew Delhi110067India Department of Biochemistry Faculty of Life SciencesUniversity of BeninBenin CityEdo-StateNigeria

Having faced increased clinical treatment failures with dihydroartemisinin-piperaquine(DHA-PPQ),Cambodia swapped the first line artemisinin-based combination therapy(ACT)from DHA-PPQ to artesunate-mefloquine given that parasites resistant to piperaquine are susceptible to ***,triple mutants have now emerged,suggesting that drug rotations may not be adequate to keep resistance at *** is,therefore,an urgent need for alternative treatment strategies to tackle resistance and prevent its spread.A proper understanding of all contributors to artemisinin resistance may help us identify novel strategies to keep artemisinins effective until new drugs become available for their *** review highlights the role of the key players in artemisinin resistance,the current strategies to deal with it and suggests ways of protecting future antimalarial drugs from bowing to resistance as their predecessors did.

关键词： Artemisinin resistance Quiescence K13 mutations Non-K13 mutations Artemisinin-based combination therapy (ACT)failure Drugs in development Malaria eradication

Cellular mechanisms regulating sperm-zona pellucida interaction

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Asian Journal of Andrology 2011年第1期13卷 88-96页

作者： Andrew T Reid Kate Redgrove R John Aitken Brett Nixon Reproductive Science group Discipline of Biological Sciences School of Environmental and Life Sciences University of Newcastle Callaghan NSW 2308 Australia ARC Centre of Excellence in biotechnology and Development Discipline of Biological Sciences School of Environmental and Life Sciences University of Newcastle CaUaghan NSW 2308 Australia

For mammalian spermatozoa to exhibit the ability to bind the zona pellucida （ZP） they must undergo three distinct phases of maturation, namely, spermatogenesis （testis）, epididymal maturation （epididymis） and capacitation （female reproductive tract）. An impressive array of spermatozoa surface remodeling events accompany these phases of maturation and appear critical for recognition and adhesion of the outer vestments of the oocyte, a structure known as the ZP. It is becoming increasingly apparent that species-specific zona adhesion is not mediated by a single receptor. Instead, compelling evidence now points toward models implicating a multiplicity of receptor-ligand interactions. This notion is in keeping with emerging research that has shown that there is a dynamic aggregation of proteins believed to be important in sperm-ZP recognition to the regions of sperm that mediate this binding event. Such remodeling may in turn facilitate the assembly of a multimeric zona recognition complex （MZRC）. Though formation of MZRCs raises questions regarding the nature of the block to polyspermy, formation and assembly of such a structure would no doubt explain the strenuous maturation process that sperm endure on their sojourn to functional maturity.

关键词： capacitation fertilization spermatozoa sperm-zona pellucida interaction

Therapeutic role of Ricinus communis L. and its bioactive compounds in disease prevention and treatment

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Asian Pacific Journal of Tropical Medicine 2018年第3期11卷 177-185页

作者： Waseem Mohammed Abdul Nahid H.Hajrah Jamal S.M.Sabir Saleh M.Al-Garni Meshaal J.Sabir Saleh A.Kabli Kulvinder Singh Saini Roop Singh Bora biotechnology research group Department of Biological SciencesFaculty of ScienceKing Abdulaziz University Department of Biological sciences Faculty of ScienceKing Abdulaziz University Department of biotechnology Eternal University

Ricinus communis L.(R. communis), commonly known as castor oil plant, is used as a traditional natural remedy or folkloric herb for the control and treatment of a wide range of diseases around the globe. Various studies have revealed the presence of diverse phytochemicals such as ***, terpenes, saponins,phenolic compounds such as kaempferol, gallic acid, ricin, rutin, lupeol, ricinoleic acid, pinene, thujone and gentisic *** phytochemicals have been responsible for pharmacological and therapeutic effects,including anticancer, antimicrobial, insecticidal, antioxidant, anti-diabetic, antinociceptive,anti-inflammatory. bone regenerative, analgesic, and anticonvulsant activity. R. communis harbours phytochemicals which have been shown to target peroxisome proliferator activated receptor(PPAR), nuclear factor NF-κ-B, cytochrome p450, P38 mitogen-activated protein kinases kinase(p38 MAPK), tumor protein P53, B-cell lymphoma-extra-large(Bcl-xL) and vascular endothelial growth factor receptor-2(VEGFR-2). Considering its wide variety of phytochemicals, its pharmacological activity and the subsequent clinical trials, R. communis could be a good candidate for discovering novel complementary drugs. Further experimental and advanced clinical studies are required to explore the pharmaceutical, beneficial therapeutic and safety prospects of R. communis with its phytochemicals as a herbal and complementary medicine for combating various diseases and disorders.

关键词： Ricinus communis Bioactive compounds Ethnomedicine Molecular targets Anti-cancer Pharmacology

Therapeutic applications of collagenase(metalloproteases): A review

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Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 975-981页

作者： Hamzeh Alipour Abbasali Raz Sedigheh Zakeri Navid Dinparast Djadid Malaria and Vector research group Biotechnology Research Center Pasteur Institute of Iran Department of Medical Entomology School of Health Research Center for Health Sciences Shiraz University of Medical Sciences

Non-invasive therapeutic methods have recently been used in medical sciences. Enzymes have shown high activity at very low concentrations in laboratories and pharmaceutical,enabling them to play crucial roles in different biological phenomena related to living organism, especially human medicine. Recently, using the therapeutic methods based on non-invasive approaches has been emphasized in medical society. researchers have focused on producing medicines and tools reducing invasive procedures in *** are proteins which catalyze chemical processes and break the peptide bonds in collagen. Collagen may be generated more than the required amount or produced in unsuitable sites or may not degrade after a certain time. In such cases, using an injectable collagenase or its ointment can be helpful in collagen degradation. In both in vitro and in vivo tests, it has been revealed that collagenases have several therapeutic properties in wound healing, burns, nipple pain and some diseases including intervertebral disc herniation, keloid, cellulite, lipoma among others. This review describes the therapeutic application of collagenase in medical sciences and the process for its production using novel methods, paving the way for more effective and safe applications of collagenases.

关键词： Enzyme Therapeutic application Collagenase Non-invasive Metalloproteinase

Long-and short-chain AHLs affect AOA and AOB microbial community composition and ammonia oxidation rate in activated sludge

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Journal of Environmental sciences 2019年第4期31卷 53-62页

作者： Jie Gao Yu Duan Ying Liu Xuliang Zhuang Yichen Liu Zhihui Bai Wenlin Ma Guoqiang Zhuang CAS Key Laboratory of Environmental biotechnology Research Center for Eco-Environmental SciencesChinese Academy of Sciences College of Resources and Environment University of Chinese Academy of Sciences Beijing Enterprises Water group Limited School of life sciences University of Science and Technology of China Beijing Climate Change Response research and Education Center Department of Environment and Energy EngineeringBeijing University of Civil Engineering and Architecture

Quorum sensing(QS)regulation of the composition of ammonia-oxidising archaea(AOA)and ammonia-oxidising bacteria(AOB)communities and functions in wastewater treatment was ***,we explored the role of N-acyl-L-homoserine lactones(AHLs)in microbial community dynamics in activated *** average,the specific ammoniaoxidising-rate increased from 1.6 to 2.8 mg NH_4^+-N/g MLSS/hr after treatment with long-chain AHLs for 16 days,and the addition of AHLs to sludge resulted in an increased number of AOA/AOB amoA *** differences were observed in the AOA communities of control and AHL-treated cultures,but not the AOB ***,the dominant functional AOA strains of the Crenarchaeota altered their ecological niche in response to AHL *** results provide evidence that AHLs play an important role in mediating AOA/AOB microbial community parameters and demonstrate the potential for application of QS to the regulation of nitrogen compound metabolism in wastewater treatment.

关键词： Quorum sensing Activated sludge Ammonia-oxidising archaea Ammonia-oxidising bacteria Nitrogen removal Community composition

Global Identification of Significantly Expressed Genes in Developing Endosperm of Rice by Expression Sequence Tags and cDNA Array Approaches

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Journal of Integrative Plant Biology 2008年第9期50卷 1078-1088页

作者： Qichao Tu Haitao Dong Haigen Yao Yongqi Fang Cheng'en Dai Hongmei Luo Jian Yao Dong Zhao Debao Li Bioinformatics and Gene Network research group Institute of Biotechnology Zhejiang University Hangzhou 310029 China Jiaxing Academy of Agricultural Science Jiaxing 314016 China College of life and Environmental sciences Hangzhou Normal University Hangzhou 310036 China

Rice endosperm plays a very important role in seedling germination and determines the qualities of rice grain. Although studies on specific gene categories in endosperm have been carried out, global view of gene expression at a transcription level In rice endosperm Is still limited. To gain a better understanding of the global and tissue-specific gene expression profiles In rice endosperm, a cDNA library from rice endosperm of immature seeds was sequenced. A cDNA array was constructed based on the tentative unique transcripts derived from expression sequence tag （EST） assembling results and then hybridized with cDNAs from five different tissues or organs including endosperm, embryo, leaf, stem and root of rice. Significant redundancy was found for genes encoding prolamin, glutelin, allergen, and starch synthesis proteins, accounting for ~34% of the total ESTs obtained. The cDNA array revealed 87 significantly expressed genes In endosperm compared with the other four organs or tissues. These genes included 13 prolamin family proteins, 17 glutelin family proteins, 12 binding proteins, nine catalytic proteins and four ribosomal proteins, indicating a complicated biological processing in rice endosperm. In addition, Northern verification of 1,4-alpha-glucan branching enzyme detected two isoforms in rice endosperm, the larger one of which only existed in endosperm.

关键词： cDNA array endosperm expression profile expression sequence tag rice (Oryza sativa)