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Leafhopper salivary carboxylesterase suppresses JA-Ile synthesis to facilitate initial arbovirus transmission in rice phloem

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Plant Communications 2024年第9期5卷 116-135页

作者： Yunhua Chi Hongxiang Zhang Siyu Chen Yu Cheng Xiaofeng Zhang Dongsheng Jia Qian Chen Hongyan Chen Taiyun Wei Vector-borne virus Research Center Fujian Province Key Laboratory of Plant VirologyInstitute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China

Plant jasmonoyl-L-isoleucine(JA-Ile)is a major defense signal against insect feeding,but whether or how insect salivary effectors suppress JA-Ile synthesis and thus facilitate viral transmission in the plant phloem remains *** carboxylesterases(CarEs)are the third major family of detoxification ***,we identify a new leafhopper CarE,CarE10,that is specifically expressed in salivary glands and is secreted into the rice phloem as a saliva *** CarE10 directly binds to rice jasmonate resistant 1(JAR1)and promotes its degradation by the proteasome ***,the direct association of CarE10 with JAR1 clearly impairs JAR1 enzyme activity for conversion of JA to JA-Ile in an in vitro JAIle synthesis system.A devastating rice reovirus activates and promotes the co-secretion of virions and CarE10 via virus-induced vesicles into the saliva-storing salivary cavities of the leafhopper vector and ultimately into the rice phloem to establish initial ***,a virus-mediated increase in CarE10 secretion or overexpression of CarE10 in transgenic rice plants causes reduced levels of JAR1 and thus suppresses JA-Ile synthesis,promoting host attractiveness to insect vectors and facilitating initial viral *** findings provide insight into how the insect salivary protein CarE10 suppresses host JA-Ile synthesis to promote initial virus transmission in the rice phloem.

关键词： leafhopper salivary protein CarE10 JAR1 degradation JA-Ile synthesis rice phloem initial viral transmission

RNA viromes of Dermacentor nuttalli ticks reveal a novel uukuvirus in Qinghai Province,China

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Virologica Sinica 2024年第4期39卷 537-545页

作者： Yaohui Fang Jun Wang Jianqing Sun Zhengyuan Su Shengyao Chen Jian Xiao Jun Ni Zhihong Hu Yubang He Shu Shen Fei Deng Key laboratory of Special Pathogens and Biosafety and National virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 101408China Qinghai Lake National Nature Reserve Administration Xining 810000China Hubei Jiangxia laboratory Wuhan 430200China Xinjiang Key laboratory of Vector-borne Infectious Diseases Urumqi830002China

Ticks are a major parasite on the Qınghai-Tibet Plateau,western China,and represent an economic burden to agriculture and animal *** research on tick-borne pathogens that threaten humans and animals,the viromes of dominant tick species in this area remain *** this study,we collected Dermacentor nuttalli ticks near Qınghai Lake and identified 13 viruses belonging to at least six families through metagenomic *** viruses were of high abundance in pools,including Xınjiang tick-associated virus 1(XJTAV1),and three novel viruses:Qınghai Lake virus 1,Qınghai Lake virus 2(QHLV1,and QHLV2,unclassified),and Qınghai Lake virus 3(QHLV3,genus Uukuvirus of family Phenuiviridae in order Bunyavirales),which lacks the M *** minimum infection rates of the four viruses in the tick groups were 8.2%,49.5%,6.2%,and 24.7%,respectively,suggesting the prevalence of these viruses in *** ticks.A putative M segment of QHLV3 was identified from the next-generation sequencing data and further characterized for its signal peptide cleavage site,N-glycosylation,and transmembrane ***,we probed the L,M,and S segments of other viruses from sequencing data of other tick pools by using the putative M segment sequence of *** revealing the viromes of *** ticks,this study enhances our understanding of tick-borne viral communities in highland *** putative M segment identified in a novel uukuvirus suggests that previously identified uukuviruses without M segments should have had the same genome organization as typical *** findings will facilitate virus discovery and our understanding of the phylogeny of tick-borne uukuviruses.

关键词： Dermacentor nuttalli Qınghai Province Virome Uukuvirus M segment

Establishment and application of a surrogate model for human Ebola virus disease in BSL-2 laboratory

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Virologica Sinica 2024年第3期39卷 434-446页

作者： Wanying Yang Wujian Li Wujie Zhou Shen Wang Weiqi Wang Zhenshan Wang Na Feng Tiecheng Wang Ying Xie Yongkun Zhao Feihu Yan Xianzhu Xia Hebei Key Lab of laboratory Animal Science Department of Laboratory Animal ScienceHebei Medical UniversityShijiazhuang050017China Key laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research InstituteChinese Academy of Agricultural SciencesChangchun130122China College of Veterinary Medicine Jilin UniversityChangchun130062China College of Veterinary Medicine Jilin Agricultural UniversityChangchun130118China

The Ebola virus(EBOV)is a member of the Orthoebolavirus genus,Filoviridae family,which causes severe hemorrhagic diseases in humans and non-human primates(NHPs),with a case fatality rate of up to 90%.The development of countermeasures against EBOV has been hindered by the lack of ideal animal models,as EBOV requires handling in biosafety level(BSL)-4 ***,accessible and convenient animal models are urgently needed to promote prophylactic and therapeutic approaches against *** this study,a recombinant vesicular stomatitis virus expressing Ebola virus glycoprotein(VSV-EBOV/GP)was constructed and applied as a surrogate virus,establishing a lethal infection in *** infection with VSV-EBOV/GP,3-week-old female Syrian hamsters exhibited disease signs such as weight loss,multi-organ failure,severe uveitis,high viral loads,and developed severe systemic diseases similar to those observed in human EBOV *** animals succumbed at 2–3 days post-infection(dpi).Histopathological changes indicated that VSV-EBOV/GP targeted liver cells,suggesting that the tissue tropism of VSV-EBOV/GP was comparable to wild-type EBOV(WT EBOV).Notably,the pathogenicity of the VSV-EBOV/GP was found to be species-specific,age-related,gender-associated,and challenge ***,equine anti-EBOV immunoglobulins and a subunit vaccine were validated using this ***,this surrogate model represents a safe,effective,and economical tool for rapid preclinical evaluation of medical countermeasures against EBOV under BSL-2 conditions,which would accelerate technological advances and breakthroughs in confronting Ebola virus disease.

关键词： Ebola virus(EBOV) Recombinant vesicular stomatitis virus Pathogenicity Syrian hamster Surrogate models Vaccine evaluation and drug screening

Viromes of Haemaphysalis longicornis reveal different viral abundance and diversity in free and engorged ticks

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Virologica Sinica 2024年第2期39卷 194-204页

作者： Jian Xiao Xuan Yao Xuhua Guan Jinfeng Xiong Yaohui Fang Jingyuan Zhang You Zhang Abulimiti Moming Zhengyuan Su Jiayin Jin Yingying Ge Jun Wang Zhaojun Fan Shuang Tang Shu Shen Fei Deng Key laboratory of Virology and Biosafety and National virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan430071China Hubei Provincial Center for Disease Control and Prevention Wuhan430070China University of Chinese Academy of Sciences Beijing101408China Hubei Jiangxia laboratory Wuhan430200China Xinjiang Key laboratory of Vector-borne Infectious Diseases Urumqi830002China Current address:Department of Medical laboratory The Second Affiliated HospitalHainan Medical UniversityHaikou57000China

Haemaphysalis longicornis ticks,commonly found in East Asia,can transmit various pathogenic viruses,includingthe severe fever with thrombocytopenia syndrome virus(SFTSV)that has caused febrile diseases among humansin Hubei ***,understanding of the viromes of *** was limited,and the prevalence ofviruses among *** ticks in Hubei was not well *** study investigates the viromes of bothengorged(fed)and free(unfed)*** ticks across three mountainous regions in Hubei Province from 2019to ***-sequencing analysis identified viral sequences that were related to 39 reference viruses belonging tounclassified viruses and seven RNA viral families,namely Chuviridae,Nairoviridae,Orthomyxoviridae,Parvoviridae,Phenuiviridae,Rhabdoviridae,and *** abundance and diversity in these ticks were analysed,andphylogenetic characteristics of the Henan tick virus(HNTV),Dabieshan tick virus(DBSTV),Okutama tick virus(OKTV),and Jingmen tick virus(JMTV)were elucidated based on their full genomic *** demonstrated that DBSTV was the most common virus found in individual *** ticks(12.59%),followed by HNTV(0.35%),whereas JMTV and OKTV were not *** results improve our understanding of *** tick viromes in central China and highlight the role of tick feeding status and geographyin shaping the viral *** findings of new viral strains and their potential impact on public health raisethe need to strengthen surveillance efforts for comprehensively assessing their spillover potentials.

关键词： Ticks Virome Haemaphysalis longicornis Phylogenetic analysis Viral abundance and diversity

Continued evolution of H10N3 influenza virus with adaptive mutations poses an increased threat to mammals

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Virologica Sinica 2024年第4期39卷 546-555页

作者： Shiping Ding Jiangtao Zhou Junlong Xiong Xiaowen Du Wenzhuo Yang Jinyu Huang Yi Liu Lihong Huang Ming Liao Jiahao Zhang Wenbao Qi State Key laboratory for Animal Disease Control and Prevention South China Agricultural UniversityGuangzhou510642China National Avian Influenza Para-Reference laboratory Guangzhou510642China Key laboratory of Zoonoses Ministry of Agriculture and Rural AffairsGuangzhou510642China National and regional Joint Engineering laboratory for Medicament of Zoonoses Prevention and Control Guangzhou510642China Key laboratory of Zoonoses Prevention and Control of Guangdong Province Guangzhou510642China State Key laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China College of Animal Science and Technology Zhongkai University of Agriculture and EngineeringGuangzhou510550China

The H10 subtype avian influenza virus(AIV)poses an ongoing threat to both birds and ***,fatal human cases of H10N3 and H10N8 infections have drawn public *** 2022,we isolated two H10N3 viruses(A/chicken/Shandong/0101/2022 and A/chicken/Shandong/0603/2022)from diseased chickens in *** analysis revealed that these viruses were genetically associated with human-origin H10N3 virus,with internal genes originating from local H9N2 *** to the H10N8 virus(A/chicken/Jiangxi/102/2013),the H10N3 viruses exhibited enhanced thermostability,increased viral release from erythrocytes,and accumulation of hemagglutinin(HA)***,we evaluated the pathogenicity of both H10N3 and H10N8 viruses in *** found that viral titers could be detected in the lungs and nasal turbinates of mice infected with the two H10N3 viruses,whereas H10N8 virus titers were detectable in the lungs and brains of ***,the proportion of double HA Q222R and G228S mutations in H10N3 viruses has increased since ***,the functional roles of the Q222R and G228S double mutations in the HA gene of H10N3 viruses remain unknown and warrant further *** study highlights the potential public health risk posed by the H10N3 virus.A spillover event of AIV to humans could be a foretaste of a looming ***,it is imperative to continuously monitor the evolution of the H10N3 influenza virus to ensure targeted prevention and control measures against influenza outbreaks.

关键词： Avian influenza virus(AIV) H10N3 Evolution Pathogenicity

Detection of ARV-Resistant Mutants in HIV-1-Infected Individuals in a Context of Systematic Switching to an Association Based on Dolutegravir in Abidjan, Côte d’Ivoire

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American Journal of Molecular Biology 2024年第3期14卷 138-151页

作者： Odegue Kpadraux Danielle Kakou-Ngazoa Solange Dechi Jean-Jacques Renaud Diallo Zelica Sina Kouamé Mireille Sylla Aboubacar Tossea Koui Stéphane Kouakou Venance Adagba Marius Apia N’Chouo Kouamé Basile Touré Offianan André Dosso Mireille Molecular Biology Platform Institut Pasteur Abidjan Cte dIvoire laboratory of the regional Blood Transfusion Center Yamoussoukro Cte dIvoire Department of Infectious and Tropical Diseases University Hospital Treichville Abidjan Cte dIvoire laboratory of the regional Blood Transfusion Center San-Pdro Cte dIvoire Department of Epidemic virus Institut Pasteur Abidjan Cte dIvoire Department of Parasitology Mycology Institut Pasteur Abidjan Cte dIvoire Department of Bacteriology-Virology Institut Pasteur Abidjan Cte dIvoire

The emergence of antiretroviral resistance mutations represents a major threat to the achievement of national and global goals for the elimination of HIV-1 infection. The global strategy in 2019 in Cte d'Ivoire is a new national policy for the management of people living with HIV with the administration of dolutegravir (DTG)-based fixed-dose combination. The aim of our study was to evaluate HIV-1 resistance to antiretrovirals (ARVs) in infected adult subjects in Cte d’Ivoire in the context of a systematic switch to a DTG-based combination. Between February 2022 and October 2023, a cross-sectional survey with random sampling was conducted in 06 services caring for people living with HIV. A total of 139 participants were included in the study. Adults with a viral load ≥ 1000 copies/mL were tested for HIV-1 ARV resistance mutations. Molecular analyses were performed using protocol of ANRS-MIE (National Agency for Research on AIDS and emerging infectious diseases). The interpretation is performed by HIVGRAD (https://***/cms/grade/). The frequencies of HIV-1 resistance to non-nucleotide reverse transcriptase inhibitors (NNRTIs), nucleotide reverse transcriptase inhibitors (NRTIs), integrase inhibitors (IINTs) and protease inhibitors (PIs) were 82%, 73%, 19% and 11% respectively. The main mutations observed in the different classes were K103N (45%), M184V (64%), E157Q (19%) and L10V/M46I/A71V/I54V (6%) respectively. This study reveals the emergence of resistance to DTG-based fixed-dose combinations, favored by high rates of resistance to NRTIs and NNRTIs. This finding underlines the need for enhanced viral load monitoring and HIV-1 genotyping tests to guide the choice of NRTIs for combination therapy. In addition, monitoring for mutations to second-generation NRTIs is essential, given the scale-up of DTG-based regimens currently underway in Cte d’Ivoire.

关键词： Resistant Mutants Dolutegravir HIV-1 Antiretrovirals Côte d’Ivoire

Overexpression of wild-type HRAS drives non-alcoholic steatohepatitis to hepatocellular carcinoma in mice

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Zoological Research 2024年第3期45卷 551-566页

作者： Chen Ling Su-Su Liu Yu-Ya Wang Gui-Tao Huo Yan-Wei Yang Nan Xu Hong Wang Yong Wu Yu-Fa Miao Rui Fu Yu-Wei Zhao Chang-Fa Fan College of Life Sciences Northwest UniversityProvincial Key Laboratory of Biotechnology of Shaanxi ProvinceXi’anShaanxi 710069China Division of Animal Model Research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China National Center for Safety Evaluation of Drugs National Institutes for Food and Drug Control(NIFDC)Beijing 100176China Division of HIV/AIDS and Sexually Transmitted virus Vaccines Institute for Biological Products ControlNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China Division of laboratory Animal Monitoring Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug Control(NIFDC)Beijing 102629China

Hepatocellular carcinoma(HCC),a prevalent solid carcinoma of significant concern,is an aggressive and often fatal disease with increasing global incidence rates and poor therapeutic *** etiology and pathological progression of non-alcoholic steatohepatitis(NASH)-related HCC is multifactorial and ***,no single animal model can accurately mimic the full NASH-related HCC pathological progression,posing considerable challenges to transition and mechanistic ***,a novel conditional inducible wild-type human HRAS overexpressed mouse model(HRAS-HCC)was established,demonstrating 100%morbidity and mortality within approximately one month under normal dietary and lifestyle *** symptoms of HCC such as ascites,thrombus,internal hemorrhage,jaundice,and lung metastasis were successfully replicated in ***-depth pathological features of NASH-related HCC were demonstrated by pathological staining,biochemical analyses,and typical marker gene *** murine anti-PD-1 and sorafenib treatment effectively prolonged mouse survival,further confirming the accuracy and reliability of the *** on protein-protein interaction(PPI)network and RNA sequencing analyses,we speculated that overexpression of HRAS may initiate the THBS1-COL4A3 axis to induce NASH with severe fibrosis,with subsequent progression to ***,our study successfully duplicated natural sequential progression in a single murine model over a very short period,providing an accurate and reliable preclinical tool for therapeutic evaluations targeting the NASH to HCC continuum.

关键词： HRAS THBS1 HCC driver factor NASH Fibrosis Cirrhosis HCC Treatment evaluation

Immune Control Strategies for Vaccinia virus-related laboratory-acquired Infections

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Biomedical and Environmental Sciences 2014年第2期27卷 142-146页

作者： WEI Qiang JIANG Meng Nan HAN Jun WANG Zi Jun Office of laboratory Management Chinese Center for Disease Control and Prevention National Institute for virus Disease Control and Prevention

While presenting biological characteristics of vaccinia virus and laboratory-acquired infections during related research processes, this paper focuses on benefits and risks of vaccinia virus immunization in relation to laboratory-acquired infections, describes characteristics and the adaptation of vaccinia virus vaccine, analyses the role vaccinia virus immunization plays in the prevention and control of laboratory-acquired infections, and finally proposes solutions and countermeasures to further promote and implement immune control strategies. The problem related to immune strategy and laboratory- acquired infections which is being raised, analyzed and explored plays an active and instructive role in vaccinia virus related researches and laboratory- acquired infections, and also helps to recommend and develop relevant immune strategy for future vaccine control of such infections.

关键词： Immune Control Strategies for Vaccinia virus-related laboratory-acquired Infections

The evolution,pathogenicity and transmissibility of quadruple reassortant H1N2 swine influenza virus in China:A potential threat to public health

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Virologica Sinica 2024年第2期39卷 205-217页

作者： Xinxin Cui Jinhuan Ma Zifeng Pang Lingzhi Chi Cuishan Mai Hanlin Liu Ming Liao Hailiang Sun College of Veterinary Medicine South China Agricultural UniversityGuangzhou510642China Key laboratory of Zoonosis Control and Prevention of Guangdong Province South China Agricultural UniversityGuangzhou510642China National and regional Joint Engineering laboratory for Medicament of Zoonosis Prevention and Control South China Agricultural UniversityGuangzhou510642China Shandong Vocational Animal Science and Veterinary College Weifang261061China Institute of Animal Health Guangdong Academy of Agricultural SciencesGuangzhou510640China

Swine are regarded as“intermediate hosts”or“mixing vessels”of influenza viruses,capable of generating strains with pandemic *** 2020 to 2021,we conducted surveillance on swine H1N2 influenza(swH1N2)viruses in swine farms located in Guangdong,Yunnan,and Guizhou provinces in southern China,as well as Henan and Shandong provinces in northern *** systematically analyzed the evolution and pathogenicity of swH1N2 isolates,and characterized their replication and transmission *** isolated viruses are quadruple reassortant H1N2 viruses containing genes from pdm/09 H1N1(PB2,PB1,PA and NP genes),triple-reassortant swine(NS gene),Eurasian Avian-like(HA and M genes),and recent human H3N2(NA gene)*** NA,PB2,and NP of SW/188/20 and SW/198/20 show high gene similarities to A/Guangdong/Yue Fang277/2017(H3N2).The HA gene of swH1N2 exhibits a high evolutionary *** five swH1N2 isolates replicate efficiently in human,canine,and swine cells,as well as in the turbinate,trachea,and lungs of mice.A/swine/Shandong/198/2020 strain efficiently replicates in the respiratory tract of pigs and effectively transmitted among ***,these current swH1N2 viruses possess zoonotic potential,highlighting the need for strengthened surveillance of swH1N2 viruses.

关键词： Swine influenza virus(SIV) H1N2 Evolution Replication Pathogenicity Zoonotic potential