RNA viromes of Dermacentor nuttalli ticks reveal a novel uukuvirus in Qinghai Province, China
作者机构：Chinese Acad Sci Key Lab Special Pathogens & Biosafety Wuhan 430071 Peoples R China Chinese Acad Sci Wuhan Inst Virol Natl Virus Resource Ctr Wuhan 430071 Peoples R China Univ Chinese Acad Sci Beijing 101408 Peoples R China Hubei Jiangxia Lab Wuhan 430200 Peoples R China Xinjiang Key Lab Vector Borne Infect Dis Urumqi 830002 Peoples R China Lake Natl Nat Reserve Adm Xining 810000 Peoples R China
出 版 物：《VIROLOGICA SINICA》 (中国病毒学（英文版）)
年 卷 期：2024年第39卷第4期
页 面：537-545页
核心收录：
学科分类：0710[理学-生物学] 0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学]
基 金：National Key R & D Program of China [2022YFC2305100, 2021YFC2300900] Key Deployment Projects of the Chinese Academy of Sciences [KJZD-SW-L11] Youth Project of the Wuhan Institute of Virology, Chinese Academy of Sciences [2023QNTJ-03] Biological Resources Program, Chinese Academy of Sciences [KFJ-BRP-017-74] National Basic Science Data Sharing Service Platform [NBSDC-DB-13]
主 题：Dermacentor nuttalli Qinghai Province Virome Uukuvirus M segment
摘 要：Ticks are a major parasite on the Qinghai-Tibet Plateau, western China, and represent an economic burden to agriculture and animal husbandry. Despite research on tick-borne pathogens that threaten humans and animals, the viromes of dominant tick species in this area remain unknown. In this study, we collected Dermacentor nuttalli ticks near Qinghai Lake and identified 13 viruses belonging to at least six families through metagenomic sequencing. Four viruses were of high abundance in pools, including Xinjiang tick-associated virus 1 (XJTAV1), and three novel viruses: Qinghai Lake virus 1, Qinghai Lake virus 2 (QHLV1, and QHLV2, unclassified), and Qinghai Lake virus 3 (QHLV3, genus Uukuvirus of family Phenuiviridae in order Bunyavirales), which lacks the M segment. The minimum infection rates of the four viruses in the tick groups were 8.2%, 49.5%, 6.2%, and 24.7%, respectively, suggesting the prevalence of these viruses in D. nuttalli ticks. A putative M segment of QHLV3 was identified from the next-generation sequencing data and further characterized for its signal peptide cleavage site, N-glycosylation, and transmembrane region. Furthermore, we probed the L, M, and S segments of other viruses from sequencing data of other tick pools by using the putative M segment sequence of QHLV3. By revealing the viromes of D. nuttalli ticks, this study enhances our understanding of tick-borne viral communities in highland regions. The putative M segment identified in a novel uukuvirus suggests that previously identified uukuviruses without M segments should have had the same genome organization as typical bunyaviruses. These findings will facilitate virus discovery and our understanding of the phylogeny of tick-borne uukuviruses.
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