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MoLrp1-mediated signaling induces nuclear accumulation of MoMsn2 to facilitate fatty acid oxidation for infectious growth of the rice blast fungus

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plant Communications 2023年第4期4卷 160-173页

作者： Ting Zhang Xingyu Wang Xue Li Ya-Nan Li Yuhe Li Shuang Wu Lele Xu Ruiwen Zhou Jing Yang Guotian Li Xinyu Liu Xiaobo Zheng Zhengguang Zhang Haifeng Zhang Department of plant pathology College of Plant ProtectionNanjing Agricultural Universityand Key Laboratory of Integrated Management of Crop Diseases and PestsMinistry of EducationNanjingChina The key laboratory of plant Immunity Nanjing Agricultural UniversityNanjingChina State key laboratory of Agricultural Microbiology Hubei Hongshan Laboratorythe Provincial Key Laboratory of Plant Pathology of Hubei ProvinceCollege of Plant Science&TechnologyHuazhong Agricultural UniversityWuhanChina

Fatty acid b-oxidation is critical for fatty acid degradation and cellular *** the rice blast fungus Magnaporthe oryzae,fatty acid b-oxidation is reported to be important mainly for turgor generation in the ***,the role of fatty acid b-oxidation during invasive hyphal growth is rarely *** demonstrated that blocking peroxisomal fatty acid b-oxidation impaired lipid droplet(LD)degradation and infectious growth of *** found that the key regulator of pathogenesis,MoMsn2,which we identified previously,is involved in fatty acid b-oxidation by targeting MoDCI1(encoding dienoyl-coenzyme A[CoA]isomerase),which is also important for LD degradation and infectious *** observations revealed that MoMsn2 accumulated from the cytosol to the nucleus during early infection or upon treatment with *** determined that the low-density lipoprotein receptor-related protein MoLrp1,which is also involved in fatty acid b-oxidation and infectious growth,plays a critical role in the accumulation of MoMsn2 from the cytosol to the nucleus by activating the cyclic AMP signaling *** results provide new insights into the importance of fatty acid oxidation during invasive hyphal growth,which is modulated by MoMsn2 and its related signaling pathways in ***.

关键词： transcription factor MoMsn2 target gene fatty acid b-oxidation cAMP signaling infectious growth

A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement

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Molecular plant 2020年第10期13卷 1420-1433页

作者： Hongxiang Zhang Jiatao Xie Yanping Fu Jiasen Cheng Zheng Qu Zhenzhen Zhao Shufen Cheng Tao Chen Bo Li Qianqian Wang Xinqiang Liu Binnian Tian David B.Collinge Daohong Jiang State key laboratory of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei Province 430070China The provincial key Lab of plant pathology of hubei province Huazhong Agricultural UniversityWuhanChina Department of plant and Environmental Sciences University of Copenhagen1871 Frederiksberg CDenmark

Mycoviruses are viruses that infect fungi,and hypovirulence-associated mycoviruses have the potential to control fungal ***,it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field,and no mycovirus has been applied for field crop *** this study,we found that a previously identified small DNA mycovirus(SsHADV-1)can convert its host,Sclerotinia sclerotiorum,from a typical necrotrophic pathogen to a beneficial endophytic ***-1 downregulates the expression of key pathogenicity factor genes in *** during *** growing in rapeseed,the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense,hormone signaling,and circadian rhythm *** a result,plant growth is promoted and disease resistance is *** experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6%and improve yield by 14.9%.Moreover,we discovered that SsHADV-1 could also infect other *** strains on DT-8-inoculated plants and that DT-8 could be recovered from dead *** findings suggest that the mycoviruses may have the ability to shape the origin of *** discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

关键词： mycovirus Sclerotinia sclerotiorum endophyte rapeseed stem rot biological control Brassica napus

Effects of ultrasonic-assisted extraction on bioactive compounds,volatile flavors and antioxidant activities of vine tea water extracts

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Traditional Medicine Research 2025年第1期10卷 73-81页

作者： Xiao-Long Zhou Wei-Jin Jiang Ji Yu Mao-Jun Yao Yun-Tong Li Hunan provincial key laboratory of Research Resource Mining and High-valued Utilization on Edible&Medicinal PlantCollege of Chemistry and Chemical EngineeringJishou UniversityJishou 416000China Hunan Gaoge Dairy Co. Ltd.Changsha 410000China

Background:Ampelopsis grossedentata,vine tea,which is the tea alternative beverages in *** vine tea processing,a large amount of broken tea is produced,which has low commercial ***:This study investigates the influence of different extraction methods(room temperature water extraction,boiling water extraction,ultrasonic-assisted room temperature water extraction,and ultrasonic-assisted boiling water extraction,referred to as room temperature water extraction(RE),boiling water extraction(BE),ultrasonic assistance at room temperature water extraction(URE),and ultrasonic assistance in boiling water extraction(UBE))on the yield,dihydromyricetin(DMY)content,free amino acid composition,volatile aroma components,and antioxidant properties of vine tea ***:A notable influence of extraction temperature on the yield of vine tea extracts(P<0.05),with BE yielding the highest at 43.13±0.26%,higher than that of RE(34.29±0.81%).Ultrasound-assisted extraction significantly increased the DMY content of the extracts(P<0.05),whereas DMY content in the RE extracts was 59.94±1.70%,that of URE reached 66.14±2.78%.Analysis revealed 17 amino acids,with L-serine and aspartic acid being the most abundant in the extracts,nevertheless ultrasound-assisted extraction reduced total free amino acid *** chromatography-mass spectrometry analysis demonstrated an increase in the diversity and quantity of compounds in the vine tea water extracts obtained through ultrasonic-assisted ***,69 and 68 volatile compounds were found in URE and UBE extracts,which were higher than the number found in RE and BE *** vitro,antioxidant activity assessments revealed varying antioxidant capacities among different extraction methods,with RE exhibiting the highest DPPH scavenging rate,URE leading in ABTS•+free radical scavenging,and BE demonstrating superior ferric ion reducing antioxidant ***:The findings suggest that extraction methods signi

关键词： vine tea dihydromyricetin ultrasonic-assisted extraction volatile aroma components

A lipid droplet-associated protein Nem1 regulates appressorium function for infection of Magnaporthe oryzae

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aBIOTECH 2023年第2期4卷 108-123页

作者： Deng Chen Xuan Cai Junjie Xing Shen Chen Juan Zhao Zhiguang Qu Guotian Li Hao Liu Lu Zheng Junbin Huang Xiao-Lin Chen State key laboratory of Agricultural Microbiology and provincial key laboratory of plant pathology of hubei province College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China State key laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125China Guangdong provincial key laboratory of High Technology for plant Protection Plant Protection Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China

Lipid droplets are important storages in fungal conidia and can be used by plant pathogenic fungi for ***,the regulatory mechanism of lipid droplets formation and the utilization during fungal development and infection are largely ***,in Magnaporthe oryzae,we identified a lipid droplet-associated protein Nem1 that played a key role in lipid droplets biogenesis and ***1 was highly expressed in conidia,but lowly expressed in appressoria,and its encoded protein was localized to lipid *** of NEM1 resulted in reduced numbers of lipid droplets and decreased content of diacylglycerol(DAG)or triacylglycerol(TAG).NEM1 was required for asexual development especially conidia ***Δnem1 mutant was nearly loss of virulence to host plants due to defects in appressorial penetration and invasive ***,Nem1 was regulated by the TOR signaling pathway and involved in the autophagy *** Ser303 residue of Nem1 could be phosphorylated by the cAMP-PKA signaling pathway and was important for biological function of ***,our study revealed a regulatory mechanism of lipid biogenesis and metabolism during the conidium and appressorium formation of the rice blast fungus.

关键词： Lipid biogenesis TOR signaling cAMP-PKA signaling Conidium formation Appressorium Magnaporthe oryzae

Enrichment of bacteria involved in the nitrogen cycle and plant growth promotion in soil by sclerotia of rice sheath blight fungus

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Stress Biology 2022年第1期2卷 299-311页

作者： Mirza Abid Mehmood Yanping Fu Huizhang Zhao Jiasen Cheng Jiatao Xie Daohong Jiang State key laboratory of Agricultural Microbiology Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China plant pathology Institute of Plant ProtectionMNS University of AgricultureMultan 60000Pakistan provincial key laboratory of plant pathology of hubei province College of Plant Science and TechnologyHuazhong Agricultural UniversityHubei ProvinceWuhan 430070China hubei Hongshan laboratory Wuhan 430070China

Rice sheath blight pathogen,Rhizoctonia solani,produces numerous sclerotia to *** a rich source of nutrients in the soil,sclerotia may lead to the change of soil *** this purpose,we amended the sclerotia of *** in soil and analyzed the changes in bacterial microbiota within the soil at different time *** the phyla level,Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Chloroflexi and Firmicutes showed varied abundance in the amended soil samples compared to those in the *** increased abundance of ammonia-oxidizing bacterium(AOB)Nitrosospira and Nitrite oxidizing bacteria(NOB)i.e.,Nitrospira was observed,where the latter is reportedly involved in the nitrifier ***,Thiobacillus,Gemmatimonas,Anaeromyxobacter and Geobacter,the vital players in denitrification,N2O reduction and reductive nitrogen transformation,respectively,depicted enhanced abundance in *** sclerotia-amended ***,asymbiotic nitrogen-fixing bacteria,notably,Azotobacter as well as Microvirga and Phenylobacterium with nitrogen-fixing potential also enriched in the amended samples compared to the *** growth promoting bacteria,such as Kribbella,Chitinophaga and Flavisolibacter also enriched in the sclerotia-amended *** per our knowledge,this study is of its kind where pathogenic fungal sclerotia activated microbes with a potential role in N transformation and provided clues about the ecological functions of *** sclerotia on the stimulation of bacterial genera involved in different processes of N-cycle within the soil in the absence of host plants.

关键词： Rice sheath blight Rhizoctonia solani Sclerotia Reductive nitrogen transformation Nitrogen fixation Soil microbiome

bZIP Transcription Factor UvATF21 Mediates Vegetative Growth,Conidiation,Stress Tolerance and Is Required for Full Virulence of Rice False Smut Fungus Ustilaginoidea virens

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Rice science 2023年第1期30卷 50-57页

作者： LIU Yueran QU Jinsong WANG Yufu YIN Weixiao LUO Chaoxi hubei key laboratory of plant pathology/College of plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China

bZIP proteins are widely distributed in eukaryotic organisms and regulate a diverse range of physiological *** bZIP proteins have previously been identified in Ustilaginoidea ***,the biological roles of these bZIP proteins in this pathogen are still ***,one of these bZIP protein coding genes,UvATF21,was functionally *** deletion of UvATF21resulted in reduced conidiation and pathogenicity despite of the increased vegetative *** deletion mutants also significantly decreased the sensitivity to osmotic and oxidative ***,deletion of UvATF21 exhibited different performances to cell wall integrity *** results indicated that UvATF21 played crucial roles in vegetative growth,conidiation,stress response,and full virulence in ***.

关键词： Ustilaginoidea virens bZIP protein conidiation stress response pathogenicity

Response of benthic algae to environmental conditions in an urban lake recovered from eutrophication, China

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Journal of Oceanology and Limnology 2020年第1期38卷 93-101页

作者： WANG Tao WANG Qing XIA Shuang YAN Chunlan PEI Guofeng hubei provincial key laboratory for Protection and Application of Special plant Germplasm in Wuling Area of China SouthCentral University for Nationalities

Benthic algae communities dominate the primary production in littoral zone of shallow *** tmderstand the long-term effect of alteration in the composition of benthic algae community assemblage in such as a lake in China,we analyzed the benthic algae developments and indicators in the Donghu Lake in Wuhan,central China in 2004 and *** compared the benthic algae biomass,compositions,and species richness of aquatic macrophytes and the changes of primary physicochemical *** results show that in the 10-year period,chl a and conductivity declined significantly but nutrient level of the whole lake remained largely *** benthic algae biomass doubled and the relative proportion of green algae increased,whereas the benthic diatom ratio *** benthic diatom assemblages and taxa differed in a number of ways,showing more epiphytic diatom species,and the relative abundance and species richness changed *** number of aquatic macrophyte species increased from 3 in 2004 to 15 in 2014,presenting a remarkable recovery from previous eutrophication *** changes of water level,chl a,and conductivity played a crucial role in governing aquatic macrophytes re-colonization in the littoral zones in the *** ecological characteristics of littoral benthic diatoms reflect habitat coupling as indicated by redundancy ***,the close link between benthic algae and macrophyte recovery demonstrates that the benthic algae metrics are much more useful than nutrient levels to quantify the process of restoration in the lake.

关键词： benthic algae benthic diatom lake restoration aquatic macrophyte water level

Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FleQ and its role in biofilm formation and root colonization

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Journal of Integrative Agriculture 2016年第2期15卷 339-348页

作者： MA Yao ZHANG Qiu-lei YANG Zhi-min LI Yun YAN Yong-liang PING Shu-zhen ZHANG Li-wen LIN Min LU Wei College of Biological Sciences China Agricultural University Biotechnology Research Institute Chinese Academy of Agricultural Sciences The key laboratory of plant pathology of hubei province/College of plant Science and Technology Huazhong Agricultural University

Flagellar biosynthesis and motility are subject to a four-tiered transcriptional regulatory circuit in Pseudomonas,and the master regulator FleQ appears to be the highest-level regulator in this hierarchical regulatory *** stutzeri A1501 is motile by a polar flagellum;however,the motility and regulatory mechanisms involved in this process are ***,we searched the A1501 genome for flagella and motility genes and found that approximately 50 genes,which were distributed in three non-contiguous chromosomal regions,contribute to the formation,regulation and function of the *** non-polar mutation of fleQ impaired flagellar biosynthesis,motility and root colonization but enhanced biofilm *** positively regulates the expression of flagellar class Ⅱ-Ⅳ genes,suggesting a regulatory cascade that is coordinated similar to that of the well-known *** on our results,we propose that flagellar genes in *** A1501 are regulated in a cascade regulated by FleQ and that flagellum-driven motility properties may be necessary for competitive rhizosphere colonization.

关键词： FleQ Pseudomonas stutzeri flagellar biosynthesis biofilm formation root colonization

Melanin,DNA replication,and autophagy affect appressorium development in Setosphaeria turcica by regulating glycerol accumulation and metabolism

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Journal of Integrative Agriculture 2022年第3期21卷 762-773页

作者： GUO Xiao-yue LIU Ning LIU Bing-hui ZHOU Li-hong CAO Zhi-yan HAN Jian-min DONG Jin-gao State key laboratory of North China Crop Improvement and Regulation/key laboratory of Hebei province for plant Physiology and Molecular pathology/Mycotoxin and Molecular plant pathology laboratory Hebei Agricultural UniversityBaoding 071001P.R.China

Setosphaeria turcica(*** turcicum)is the pathogenic fungus of maize(Zea mays)that causes northern leaf blight,which is a major maize disease *** appressoria are highly specialized infection structures formed by germinated conidia of *** that infect maize *** appressorium penetrates the plant cuticle by generating turgor,and glycerol is known to be the main source of the ***,the infection position penetrated by the appressorium on maize leaves was investigated,most of the germinated conidia entered the leaf interior by directly penetrating the epidermal cells,and the appressorium structure was necessary for the infection,whether it occurred through epidermal cells or ***,to investigate the effects of key factors in the development of the appressorium,we studied the effects of three inhibitors,including a melanin inhibitor(tricyclazole,TCZ),a DNA replication inhibitor(hydroxyurea,HU),and an autophagy inhibitor(3-methyladenine,3-MA),on appressorium turgor and glycerol *** results,appressorium turgor pressure and glycerol concentration in the appressorium reached their highest levels at the mature stage of the appressorium under the control and inhibitor *** three inhibitors had the greatest effects on appressorium turgor pressure at this *** and liposomes are the main substances producing *** was also found inhibitors affected the distribution of glycogen and liposomes,which were detected in the conidia,the germ tube,and the appressorium during appressorium *** study provides profound insight into the relationship between appressorium turgor pressure and glycerol content,which was affected by the synthesis of melanin,DNA replication,and autophagy in the developing appressorium during a *** infection.

关键词： melanin DNA replication autophagy appressorium development Setosphaeria turcica glycerol