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Journal of Sport and Health Science 2024年第5期13卷 666-669页

作者： Terese L.Chmielewski Adam Meierbachtol Rick Aberman Travis Gunderson Jonathon Sikorski Kathleen Cummer TRIA Orthopedic center BloomingtonMN 55431USA Rehabilitation Science Graduate Program Department of Family Medicine and Community HealthMedical SchoolUniversity of MinnesotaMinneapolisMN 55455USA Optum inc. Clinical OperationsPhysical Health DivisionEden PrairieMN 55344USA Dr.Rick Aberman Consulting MinnetonkaMN 55305USA Department of Rehabilitation Medicine Division of Physical TherapyUniversity of WashingtonSeattleWA 98195USA

Helping athletes return to sports participation is a primary goal of anterior cruciate ligament(ACL)reconstruction *** facilitate the achievement of this goal,decades of research studies have sought to identify knee impairments that reduce knee function as well as interventions to resolve them.1Yet,over the past 10 years,research pertaining to psychological responses(i.e.,cognitions and emotions)after ACL reconstruction has grown exponentially—a phenomenon that can be visualized by entering the search terms"psychological"and"anterior cruciate ligament reconstruction"into the PubMed search engine.

关键词： ligament return psychological

Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain:One of the predominant strains circulating in China from 2017 to 2021

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Virologica Sinica 2022年第5期37卷 646-655页

作者： Xiaowen Li Yang Li Jiapei Huang Yali Yao Wenying Zhao Yunjing Zhang Jie Qing Jing Ren Zhong Yan Zewei Wang Xiaofang Hu Duli Kang Hongqiang Liu Zhichun Yan New Hope Liuhe Co. Ltd.Chengdu610041China National Research center for Veterinary Medicine Luoyang471000China Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co. Ltd.(NHLH Academy of Swine Research)Dezhou253034China swine Health Data and Intelligent Monitoring Project Laboratory Dezhou UniversityDezhou253011China Pulike Biological Engineering inc. Luoyang471000China

Porcine epidemic diarrhea(PED)caused by porcine epidemic diarrhea virus(PEDV)is one of the most devastating diseases in the global pig industry due to its high mortality rate in *** vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV *** 2017 to 2021,we collected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of *** result showed that about 52.15%(158/303)of the farms were positive for PEDV with an overall detection rate of 63.95%(564/882)of the *** S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis.A total of 71 PEDV strains(68.27%)sequenced in this study were clustered into the predominant G2c subgroup,while the newly-defined G2d strains(9.62%)were identified in three provinc.s of *** NH-TA2020 strain of G2c subgroup was isolated and cultured,and its infection to piglets caused watery diarrhea within 24 h,indicating its strong *** administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in *** piglets fed by the gilts above were challenged with NH-TA2020 strain or CH-HeB-RY-2020 strain from G2d subgroup,and the clinical symptoms and virus shedding were significantly reduced compared to the mock *** findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to *** administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses,which confer protection against the homologous and emerging G2d PEDV strains challenges in neonates.

关键词： Porcine epidemic diarrhea virus(PEDV) Molecular epidemiological investigation Virus isolation Lactogenic immune IgA

Nutritional and Metabolic Consequences of Feeding High-Fiber Diets to swine： A Review

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Engineering 2017年第5期3卷 716-725页

作者： Atta K. Agyekum C. Martin Nyachoti prairie swine center inc. Saskatoon SK S7H 5N9 Canada Department of Animal Science University of Manitoba Winnipeg MB R3T 2N2 Canada

目前,大量的低成本、纤维类的副产物被添加到猪的日粮中以减少猪的养殖成本。然而,由于猪不能有效降解膳食纤维,因此富含纤维的日粮营养价值偏低。此外,高纤维日粮会降低营养物质的利用率和猪的生长性能。最近的研究结果不一致,甚至互... 详细信息

目前,大量的低成本、纤维类的副产物被添加到猪的日粮中以减少猪的养殖成本。然而,由于猪不能有效降解膳食纤维,因此富含纤维的日粮营养价值偏低。此外,高纤维日粮会降低营养物质的利用率和猪的生长性能。最近的研究结果不一致,甚至互相矛盾,而且纤维来源、纤维类型和纤维水平也会影响高纤维日粮的负效应。另外,测定膳食纤维及其纤维组成的分析方法会经常干扰纤维对猪生长和生理反应的影响。虽然已经有一些改善纤维对猪的消极影响、提高日粮的营养价值的方法被逐渐应用,如外源纤维降解酶广泛应用于提高营养物质利用率和猪的生长性能。但是,与研究结果并不一致,还需要阐明外源纤维降解酶在猪的代谢和生理反应中的作用方式。另外,膳食纤维日益成为促进猪的肠道健康和改善妊娠母猪福利的方法。本文对膳食纤维及其对猪的营养、肠道生理和母猪福利的影响进行了讨论。同时,本文建议要对膳食纤维和外源酶如何提高猪对高纤维原料的利用率方面进行深入的研究。

关键词：肠道生理高纤维日粮营养物质利用猪母猪福利

Effect of dietary fiber and threonine content on intestinal barrier function in pigs challenged with either systemic *** lipopolysaccharide or enteric Salmonella Typhimurium

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Journal of Animal Science and Biotechnology 2020年第3期11卷 724-735页

作者： Michael O.Wellington Kimberley Hamonic Jack E.C.Krone John K.Htoo Andrew G.Van Kessel Daniel A.Columbus prairie swine Centre Inc.SaskatoonSK S7H 5N9Canada Department of Animal and Poultry Science University of SaskatchewanSaskatoonSK S7N 5A8Canada Evonik Nutrition&Care GmbH Hanau-WolfgangGermany

Background:The independent and interactive effects of dietary fiber(DF)and threonine(Thr)were investigated in growing pigs challenged with either systemic *** lipopolysaccharide(LPS)or enteric Salmonella Typhimurium(ST)to characterise their effect on intestinal barrier ***:In experiment 1,intestinal barrier function was assessed via oral lactulose and mannitol(L:M)gavage and fecal mucin analysis in pigs challenged with *** LPS and fed low fiber(LF)or high fiber(HF)diets with graded dietary *** lactulose recovery and L:M ratio inc.eased(Pinc.eased(Pinc.eased(Pinc.eased fecal mucin output in the HF-fed pigs(Fib×Thr;Pinc.eased(Pinc.eased MUC2 expression with the HF *** HF inc.eased goblet cell numbers(Pinc.eased mucin production capacity(i.e.,goblet cell numbers,MUC2 gene expression)and secretion(i.e.,fecal mucin output).The lack of effect of dietary Thr in Salmonella-challenged pigs provides further evidence that mucin secretion in the gut is conserved and,therefore,Thr may be limiting for growth under conditions of inc.eased mucin production.

关键词： Barrier function E.coli lipopolysaccharide Fiber Goblet cells Mucin Salmonella swine Threonine

ACOUSTIC MEASUREMENTS BUBBLES IN BIOLOGICAL TIESSURE

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Journal of Hydrodynamics 2009年第1期21卷 47-64页

作者： CHAHINE Georges L. TANGUAY Michel LORAINE Greg DYNAFLOW INC. 10621-J Iron Bridge Road Jessup Maryland 20794 USA

An acoustic based instrument, the ABS Acoustic Bubble Spectrometer@@（ABS）, was investigated for the detection and quantification of bubbles in biological media. These inc.ude viscoelastic media （blood）, materials of varying density （bone in tissue）, non-homogenous distribution of bubbles （intravenous bubbly flow）, and bubbles migrating in tissue （decompression sickness, DCS）. The performance of the ABS was demonstrated in a series of laboratory experiments. Validation of the code was performed using a viscoelastic polymer solution, Polyox, in which the bubble size distribution and void fraction were determined by ABS measurements and with image analysis of high speed videos. These tests showed that the accuracy of the ABS was not significantly affected by viscoelasticity for bubbles smaller than 200 microns. The ABS detection and measurement of non-homogenous bubble distributions was demonstrated using a bubbly flow through a simulated vein surrounded by tissue. The scatter of acoustic signals due to bones in the acoustic pathway was also investigated. These in-vitro experiments were done using meat （beef） as a tissue simulant. Decompression experiments were done using beef meat which was held underwater at high pressure （9.9 atm） then rapidly decompressed. Bubble size distributions and void fraction calculations in these experiments were then validated using image analysis of high speed video. In addition, preliminary experiments were performed with the US Navy Medical Research center, demonstrating the utility of the modified ABS system in detecting the evolution of bubbles in swine undergoing decompression sickness （DCS）. These results indicate that the ABS may be used to detect and quantify the evolution of bubbles in-vivo and aid in the monitoring of DCS.

关键词： ABS Acoustic Bubble Spectrometer@@ (ABS) biological tissue decompression sickness (DCS)

Essential oils as alternatives to antibiotics in swine production

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Animal Nutrition 2018年第2期4卷 126-136页

作者： Faith A.Omonijo Liju Ni Joshua Gong Qi Wang Ludovic Lahaye Chengbo Yang Department of Animal Science University of Manitoba Shanghai Lab-Animal Research center Guelph Food Research Centre Agriculture and Agri-Food Canada Jefo Nutrition inc.

This review article summarizes the efficacy, feasibility and potential mechanisms of the application of essential oils as antibiotic alternatives in swine *** there are numerous studies demonstrating that essential oils have several properties, such as antimicrobial, antioxidative and antiinflammatory effects, feed palatability enhancement and improvement in gut growth and health, there is still a need of further investigations to elucidate the mechanisms underlying their *** the past,the results has been inc.nsistent in both laboratory and field studies because of the varied product compositions, dosages, purities and growing stages and conditions of *** minimal inhibitory concentration(MIC) of essential oils needed for killing enteric pathogens may not ensure the optimal feed intake and the essential oils inc.usion cost may be too high in swine *** the lipophilic and volatile nature of essential oils, there is a challenge in effective delivery of essential oils within pig gut and this challenge can partially be resolved by microencapsulation and *** effects of essential oils on inflammation, oxidative stress, microbiome, gut chemosensing and bacterial quorum sensing(QS) have led to better production performance of animals fed essential oils in a number of *** has been demonstrated that essential oils have good potential as antibiotic alternatives in feeds for swine *** combination of different essential oils and other compounds(synergistic effect) such as organic acids seems to be a promising approach to improve the efficacy and safety of essential oils in ***-throughput systems technologies have been developed recently,which will allow us to dissect the mechanisms underlying the functions of essential oils and facilitate the use of essential oils in swine production.

关键词： Essential oils Organic acids Medium chain fatty acids Inflammation Oxidative stress Pigs

The potential of genetically-engineered pigs in providing an alternative source of organs and cells for transplantation

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The Journal of Biomedical Research 2013年第4期27卷 249-253页

作者： David K.C.Cooper Hidetaka Hara Mohamed Ezzelarab Rita Bottino Massimo Trucco Carol Phelps David Ayares Yifan Dai Thomas E.Starzl Transplantation Institute University of Pittsburgh Medical Center Division of Immunogenetics Children's Hospital of Pittsburgh University of Pittsburgh Revivicor Inc Jiangsu Key Laboratory of Xenotransplantation Nanjing Medical University

There is a critical shortage of organs, cells, and corneas from deceased human donors worldwide. There are also shortages of human blood for transfusion. A potential solution to all of these problems is the transplantation of organs, cells, and corneas from a readily available animal species, such as the pig, and the transfusion of red blood cells from pigs into humans. However, to achieve these ends, major immunologic and other barriers have to be overcome. Considerable progress has been made in this respect by the genetic modification of pigs to protect their tissues from the primate immune response and to correct several molecular inc.mpatibilities that exist between pig and primate. These have inc.uded knockout of genes responsible for the expression of major antigenic targets for primate natural anti-pig antibodies, insertion of human complement- and coagulation-regulatory transgenes, and knockdown of swine leukocyte antigens that stimulate the primate＇s adaptive immune response. As a result of these manipulations, the administration of novel immunosuppressive agents, and other innovations, pig hearts have now functioned in baboons for 6-8 months, pig islets have maintained normoglycemia in diabetic monkeys for 〉 1 year, and pig corneas have maintained transparency for several months. Clinical trials of pig islet trans- plantation are already in progress. Future developments will involve further genetic manipulations of the organ- source pig, with most of the genes that are likely to be beneficial already identified.

关键词： pig blood transfusion pig genetic-engineered pig islets pig organs xenotransplantation

PA基因影响欧亚类禽型H1N1猪流感病毒致病性和传播能力的机制

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中国预防兽医学报 2022年第9期44卷 1023-1023页

作者： MENG FEI YANG HUANG-LIANG QU ZHI-YUAN State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001 People's Republic of China. Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642 People's Republic of China. Division of Virology Department of Microbiology and Immunology International Research Center for Infectious Diseases Institute of Medical Science University of Tokyo Tokyo 108-8639 Japan. The Research center for Global Viral Diseases National Center for Global Health and Medicine Research Institute Tokyo 162-8655 Japan. Influenza Research Institute Department of Pathobiological Sciences School of Veterinary Medicine University of Wisconsin-Madison Madison WI 53711.

不同宿主来源的流感病毒在猪体内经基因重配后可以产生新型病毒,解析新型流感病毒生物学表型变化机制对保护动物健康和预防人类流感大流行具有重要意义。近日,美国科学院院报《PNAS》杂志在线发表了题为“A Eurasian avian-like H1N1 sw... 详细信息

不同宿主来源的流感病毒在猪体内经基因重配后可以产生新型病毒,解析新型流感病毒生物学表型变化机制对保护动物健康和预防人类流感大流行具有重要意义。近日,美国科学院院报《PNAS》杂志在线发表了题为“A Eurasian avian-like H1N1 swine influenza reassortant virus became pathogenic and highly transmissible due to mutations in its PA gene”的研究论文,揭示了猪流感病毒(SIV)致病性和传播能力增强的分子机制。

关键词：动物健康流感病毒美国科学院 H1N1 新型病毒致病性