Isolation and oral immunogenicity assessment of porcine epidemic diarrhea virus NH-TA2020 strain:One of the predominant strains circulating in China from 2017 to 2021

作     者：Xiaowen Li Yang Li Jiapei Huang Yali Yao Wenying Zhao Yunjing Zhang Jie Qing Jing Ren Zhong Yan Zewei Wang Xiaofang Hu Duli Kang Hongqiang Liu Zhichun Yan Xiaowen Li;Yang Li;Jiapei Huang;Yali Yao;Wenying Zhao;Yunjing Zhang;Jie Qing;Jing Ren;Zhong Yan;Zewei Wang;Xiaofang Hu;Duli Kang;Hongqiang Liu;Zhichun Yan

作者机构：New Hope Liuhe Co.Ltd.Chengdu610041China National Research Center for Veterinary MedicineLuoyang471000China Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co.Ltd.(NHLH Academy of Swine Research)Dezhou253034China Swine Health Data and Intelligent Monitoring Project LaboratoryDezhou UniversityDezhou253011China Pulike Biological Engineering Inc.Luoyang471000China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2022年第37卷第5期

页      面：646-655页

核心收录：

学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Key R&D Program of China(2021ZD0113803) the"Pioneer"and"Leading Goose"R&D Program of Zhejiang (2022C02031) the National Natural Science Foundation of China (No. 31701424) 

主　　题：Porcine epidemic diarrhea virus(PEDV) Molecular epidemiological investigation Virus isolation Lactogenic immune IgA 

摘      要：Porcine epidemic diarrhea(PED)caused by porcine epidemic diarrhea virus(PEDV)is one of the most devastating diseases in the global pig industry due to its high mortality rate in piglets.Maternal vaccines can effectively enhance the gut-mammary gland-secretory IgA axis to boost lactogenic immunity and passive protection of nursing piglets against PEDV challenge.From 2017 to 2021,we collected 882 diarrhea samples from 303 farms in China to investigate the epidemiology of PEDV.The result showed that about 52.15%(158/303)of the farms were positive for PEDV with an overall detection rate of 63.95%(564/882)of the samples.The S1 fragments of S gene from 104 strains were sequenced for the phylogenetic analysis.A total of 71 PEDV strains(68.27%)sequenced in this study were clustered into the predominant G2c subgroup,while the newly-defined G2d strains(9.62%)were identified in three provinces of China.The NH-TA2020 strain of G2c subgroup was isolated and cultured,and its infection to piglets caused watery diarrhea within 24 h,indicating its strong pathogenicity.Oral administration of NH-TA2020 strain to pregnant gilts stimulated high levels of IgA antibody in colostrum.The piglets fed by the gilts above were challenged with NH-TA2020 strain or CH-HeB-RY-2020 strain from G2d subgroup,and the clinical symptoms and virus shedding were significantly reduced compared to the mock group.Our findings suggest that G2c subgroup is the predominant branch circulating in China from 2017 to 2021.Oral administration of NH-TA2020 enhances maternal IgA and lactogenic immune responses,which confer protection against the homologous and emerging G2d PEDV strains challenges in neonates.