检索结果-南通市图书馆

Analysis of the function of D279N mutation of VP2 of infectious bursal disease virus

Journal of Integrative Agriculture 2015年第12期14卷 2618-2625页

作者： QI Xiao-le LU Zhen WANG Nian CHEN Yu-ming ZHANG Li-zhou GAO Li LI Kai REN Xian-gang WANG Yong-qiang GAO Hong-lei GAO Yu-long Nicolas Eterradossi WANG Xiao-mei Division of Avian infectious diseases State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Avian and Rabbit Virology Immunology and Parasitology Unit OIE Reference Laboratory for Infectious Bursal Disease French Agency for Food Environmental and Occupational Health Safety (Anses) Jiangsu Co-Innovation Center for Prevention and Control of Important Animal infectious disease and Zoonoses

infectious bursal disease virus（IBDV）is responsible for the highly contagious infectious bursal disease of *** understanding the gene-function is necessary to design the tailored *** amino acid residue 279,located on strand P_F of VP2,is one of the three residues that have been reported to be involved in cell-tropism but with some *** this study,to further clarify the amino acids involved in the cell tropism of IBDV,a series of mutations about residue 279were introduced into the VP2 of vv IBDV Gx *** the reverse genetic system,we found single mutation of D279N,double mutations of D279N/A284T or Q253H/D279N were not enough to adapt IBDV to chicken embryo fibroblast（CEF）*** evaluate whether residue 279 could influence the replication and virulence of IBDV,the virus r Gx HT-279 with three mutations（Q253H/D279N/A284T）was rescued and *** showed that the mutation of residue 279 in VP2had no efficient effects on both the replication efficiency in vitro and the virulence to SPF chickens of *** summary,the results demonstrated that residue 279 of VP2 did not contribute efficiently to cell tropism,replication efficiency,and virulence of IBDV at least in some *** findings provided further information for understanding the gene function of IBDV.

关键词： infectious bursal disease virus(IBDV) residue 279 cell tropism virulence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rhabdomyolysis-related acute kidney injury in COVID-19:A critical concern

引用

World Journal of Virology 2025年第1期14卷 127-129页

作者： Md Safiullah Sarker Virology laboratory Infectious Diseases DivisionDhaka 1212Bangladesh

Rhabdomyolysis is a severe condition characterized by the breakdown of muscle tissue leading to the release of intracellular components into the *** condition,when associated with acute kidney injury(AKI),can result in significant morbidity and mortality,particularly in the context of coronavirus disease 2019(COVID-19).This editorial discusses a retrospective study on patients with COVID-19 who developed rhabdomyolysis-related *** study highlights that patients with rhabdomyolysis exhibited higher inflammatory markers,such as Creactive protein,ferritin,and procalcitonin,and experienced worse clinical outcomes compared to those with other causes of *** findings underscore the importance of early recognition and management of rhabdomyolysis in COVID-19 patients to improve prognosis and reduce mortality rates.

关键词： Rhabdomyolysis Acute kidney injury COVID-19 SARS-CoV-2 Creatine kinase Inflammation Prognosis Mortality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An improved scheme for infectious bursal disease virus genotype classification based on both genome-segments A and B

引用

Journal of Integrative Agriculture 2021年第5期20卷 1372-1381页

作者： WANG Yu-long FAN Lin-jin JIANG Nan GAO Li LI Kai GAO Yu-long LIU Chang-jun CUI Hong-yu PAN Qing ZHANG Yan-ping WANG Xiao-mei QI Xiao-le Avian Immunosuppressive diseases Division State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China oie reference laboratory for infectious bursal disease Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China Jiangsu Co-innovation Center for Prevention and Control of Important Animal infectious disease and Zoonosis Yangzhou 225009P.R.China

infectious bursal disease(IBD)is caused by infectious bursal disease virus(IBDV),which has a genome consisting of two segments of double-stranded linear *** have been traditionally divided into four phenotypes based on their pathogenicity and antigenicity,including classic,variant,very virulent,and attenuated *** the emergences of divergent molecular characteristics of novel strains produced by continuous mutations and recombination,it is increasingly difficult to define new IBDV strains using the traditional descriptive classification *** most common classification scheme for IBDV with segmented genome is based solely on segment A,while the significance of segment B has been largely *** this study,an improved scheme for IBDV genotype classification based on the molecular characteristics of both VP2(a viral capsid protein encoded by segment A)and VP1(an RNA-dependent RNA polymerase protein encoded by segment B)was proposed for the first *** this scheme,IBDV was classified into nine genogroups of A and five genogroups of B,respectively;the genogroup A2 was further divided into four *** commonly used phenotypic classifications of classic,variant,very virulent,and attenuated IBDVs correspond to the A1 B1,A2 B1,A3 B2,and A8 B1 genotypes of the proposed classification *** novel variant IBDVs including the strains identified in this study were classified as belonging to genotype A2 d *** flexibility and versatility of this improved classification scheme will allow the unambiguous identification of existing and emerging IBDV strains,which will greatly facilitate molecular epidemiology studies of IBDV.

关键词： infectious bursal disease virus genotype VP1 VP2 novel variant strain

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pathogenic Characterization and Full Length Genome Sequence of a Reassortant infectious bursal disease Virus Newly Isolated in Pakistan

引用

Virologica Sinica 2019年第1期34卷 102-105页

作者： Altaf Hussain Tiantian Wu Hui Li Linjin Fan Kai Li Li Gao Yongqiang Wang Yulong Gao Changjun Liu Hongyu Cui Qing Pan Yanping Zhang Asim Aslam Khan Muti-Ur-Rehman Muhammad Munir Salman Latif Butt Xiaomei Wang Xiaole Qi Division of Avian infectious diseases State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteThe Chinese Academy of Agricultural Sciences oie reference laboratory for infectious bursal disease Pathology Department University of Veterinary and Animal Sciences Division of Biomedical and Life Sciences Faculty of Health and MedicineLancaster University Department of Pathology College of Veterinary MedicineUniversity of Georgia Jiangsu Co-innovation Centre for Prevention and Control of Important Animal infectious disease and Zoonoses

Dear Editor,infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a nonenveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan,poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry ***.

关键词： In Pathogenic Characterization and Full Length Genome Sequence of a Reassortant infectious bursal disease Virus Newly Isolated in Pakistan

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Approach from the laboratory to the field: New strategies in the control of mosquito-borne infectious diseases

引用

Science China Life Sciences 2025年第01期 303-305页

作者： Ziqian Xu Lianpan Dai Jun Liu National Key laboratory of Intelligent Tracking and Forecasting for infectious diseases (NITFID) National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention CAS Key laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences

Vector-borne viruses, transmitted among humans and animal hosts through bites of vectors such as mosquitoes and ticks,pose a great and ongoing threat to public health and biosafety(Yang et al., 2022).Among them, dengue virus-induced epidemics, have dramatically increased worldwide in recent decades. The report released by the World Health Organization(WHO) at the end of 2023 showed that dengue epidemic globally surged in2023, with the number of reported cases nearing a historical peak.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs

引用

Virologica Sinica 2021年第2期36卷 187-195页

作者： Dan Li Yinguang Liu Xiaolan Qi Yuan Wen Pan Li Zhao Ma Yongjie Liu Haixue Zheng Zhijie Liu State Key laboratory of Veterinary Etiological Biology and oie/National Foot and Mouth disease reference laboratory Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730046China

African swine fever virus(ASFV)is the etiological agent of African swine fever(ASF),an often lethal disease in domestic and wild *** represents a major threat to the swine industry ***,no commercial vaccine is available because of the complexity of ASFV or biosecurity *** attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain *** the present study,a mutant ASFV strain with the deletion of ASFV MGF-110-9L(ASFV-D9L)was generated from a highly virulent ASFV CN/GS/2018 parental strain,a genotypeⅡ*** to the parental ASFV isolate,deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-D9L to replicate in vitro in primary swine macrophage cell *** majority of animals inoculated intramuscularly with a low dose of ASFV-D9L(10 HAD50)remained clinically normal during the 21-day observational *** of five ASFV-D9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response,indicating partial attenuation of the ASFV-D9L strain in *** findings imply the potential usefulness of the ASFV-D9L strain for further development of ASF control measures.

关键词： African swine fever virus(ASFV) MGF-110-9L Mutant Attenuated virulence Pig

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

C-X-C chemokine receptor type 5+CD8+T cells as immune regulators in hepatitis Be antigen-positive chronic hepatitis B under interferonalpha treatment

引用

World Journal of Gastroenterology 2025年第3期31卷 73-83页

作者： Zhen-Yu Xu Zhong-Shang Dai Guo-Zhong Gong Min Zhang Department of infectious diseases Second Xiangya HospitalCentral South UniversityChangsha 410011Hunan ProvinceChina Institute of Hepatology and Department of infectious diseases The Second Xiangya HospitalCentral South UniversityChangsha 410011Hunan ProvinceChina

BACKGROUND C-X-C chemokine receptor type 5(CXCR5)+CD8+T cells represent a unique immune subset with dual roles,functioning as cytotoxic cells in persistent viral infections while promoting B cell *** their importance,the specific role of CXCR5+CD8+T cells in chronic hepatitis B(CHB),particularly during interferon-alpha(IFN-α)treatment,is not fully *** study aims to elucidate the relationship between CXCR5+CD8+T cells and sustained serologic response(SR)in patients undergoing 48 weeks of pegylated IFN-α(peg-IFN-α)treatment for *** To elucidate the relationship between CXCR5+CD8+T cells and sustained SR in patients undergoing 48 weeks of peg-IFN-αtreatment for *** This study enrolled 60 patients with hepatitis Be antigen(HBeAg)-positive CHB undergoing 48 weeks of peg-IFN-α*** were assessed for eligibility based on criteria such as persistent HBsAg-positive status for at least six months,HBeAb-negative,hepatitis B virus DNA levels exceeding 2×104 copies/mL,and alanine aminotransferase(ALT)levels between 2 and 10 times the upper limit of *** samples were collected at baseline and at weeks 12,24,48,and a 24-week treatment-free follow-up(week 72)to measure serum interleukin(IL)-21 concentration via ELISA and to analyze CXCR5 and programmed death-ligand 1(PD-L1)expression on CD8+T cells by flow cytometry,CXCR5 is a chemokine receptor that directs immune cells to specific tissues,while PD-L1 is a protein that regulates immune responses by inhibiting T cell *** Patients with CHB exhibited significantly lower levels of circulating CXCR5+CD8+T cells compared to healthy controls(P<0.01).Notably,CXCR5+CD8+T cells were prominently expressed in patients who achieved sustained SR compared to non-SR(NSR).A significant correlation was observed between CXCR5 and PD-L1 expression(r=-0.189,P=0.002).However,there was no significant correlation between serum IL-21 levels and CXCR5+CD8+lymphocytes(r=-0.03,P=0.62

关键词： C-X-C chemokine receptor type 5 Programmed death-ligand 1 Interleukin-21 Pegylated interferon-alpha Chronic hepatitis B

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Challenges and needs for China to eliminate rabies

引用

infectious diseases of Poverty 2013年第1期2卷 178-187页

作者： Wenwu Yin Jie Dong Changchun Tu John Edwards Fusheng Guo Hang Zhou Hongjie Yu Sirenda Vong Division of infectious diseases Chinese Center for Disease Control and PreventionBeijingChina Key laboratory of Surveillance and Early-warning on infectious disease Chinese Center for Disease Control and PreventionBeijingChina World Health Organization Country OfficeBeijingChina oie reference laboratory for Rabies Institute of Military VeterinaryAcademy of Military Medical SciencesChangchunChina Food and Agriculture Organization BeijingChina National Institute for viral disease control and prevention Chinese Center for Disease Control and PreventionBeijingChina

In China,rabies is a significant public health concern where dogs remain the main reservoir of disease transmission to humans;rabies-related mortality ranks second in the *** compiled all published articles and official documents on rabies in China's Mainland to examine challenges and needs to eliminate rabies in the *** Chinese authorities have identified rabies as a priority,recognized rabies control in dogs as key to control rabies in humans and required intersectoral *** have been made to respond effectively to the latest re-emergence of rabies,which peaked in 2007 with>3,300 *** these outcomes and the increasing volume of publications and regulations in the recent years,our review points to some major information gaps to improve rabies control activities and envisage elimination *** emphasis on laboratory or pathogen-associated and basic epidemiology research in the literature has contrasted with the absence of information to monitor various systems in humans and animals(*** of surveillance,response and post-exposure prophylaxis).Information is also lacking to appropriately inform policymakers(*** disease burden,impact of policies)and assist program managers(*** and strategic guidance for cost-effective prevention and control activities,public education and dog population management).In conclusion,strategic planning is needed to provide a sense of direction,demonstrate feasibility of elimination in China,and develop a research agenda,addressing country’s operational needs and *** planning should be a multisectoral effort.

关键词： Rabies Elimination China Needs Challenges Review

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transforming growth factor-beta 1 enhances discharge activity of cortical neurons

引用

Neural Regeneration Research 2025年第2期20卷 548-556页

作者： Zhihui Ren Tian Li Xueer Liu Zelin Zhang Xiaoxuan Chen Weiqiang Chen Kangsheng Li Jiangtao Sheng Department of Microbiology and Immunology Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular ImmunopathologyShantou University Medical CollegeShantouGuangdong ProvinceChina Department of Neurosurgery First Affiliated Hospital of Shantou University Medical CollegeShantouGuangdong ProvinceChina

Transforming growth factor-beta 1(TGF-β1)has been extensively studied for its pleiotropic effects on central nervous system *** neuroprotective or neurotoxic effects of TGF-β1 in specific brain areas may depend on the pathological process and cell types ***-gated sodium channels(VGSCs)are essential ion channels for the generation of action potentials in neurons,and are involved in various neuroexcitation-related ***,the effects of TGF-β1 on the functional properties of VGSCs and firing properties in cortical neurons remain *** this study,we investigated the effects of TGF-β1 on VGSC function and firing properties in primary cortical neurons from *** found that TGF-β1 increased VGSC current density in a dose-and time-dependent manner,which was attributable to the upregulation of Nav1.3 *** VGSC current density and Nav1.3 expression were significantly abolished by preincubation with inhibitors of mitogen-activated protein kinase kinase(PD98059),p38 mitogen-activated protein kinase(SB203580),and Jun NH2-terminal kinase 1/2 inhibitor(SP600125).Interestingly,TGF-β1 significantly increased the firing threshold of action potentials but did not change their firing rate in cortical *** findings suggest that TGF-β1 can increase Nav1.3 expression through activation of the ERK1/2-JNK-MAPK pathway,which leads to a decrease in the firing threshold of action potentials in cortical neurons under pathological ***,this contributes to the occurrence and progression of neuroexcitatory-related diseases of the central nervous system.

关键词： central nervous system cortical neurons ERK firing properties JNK Nav1.3 p38 transforming growth factor-beta 1 traumatic brain injury voltage-gated sodium currents

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A New High-throughput Real-time PCR Assay for the Screening of Multiple Antimicrobial Resistance Genes in Broiler Fecal Samples from China

引用

Biomedical and Environmental Sciences 2019年第12期32卷 881-892页

作者： CHE Jie LU Jin Xing LI Wen Ge ZHANG Yun Fei ZHAO Xiao Fei YUAN Min BAI Xue Mei CHEN Xia LI Juan State Key laboratory of infectious disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention

Objective Antimicrobial resistance(AMR)has become a global concern and is especially severe in *** effectively and reliably provide AMR data,we developed a new high-throughput real-time PCR assay based on microfluidic dynamic technology,and screened multiple AMR genes in broiler fecal *** A high-throughput real-time PCR system with an new designed integrated fluidic circuit assay were performed AMR gene detection.A total of 273 broiler fecal samples collected from two geographically separated farms were screened AMR *** The new assay with limits of detection ranging from 40.9 to 8,000 copies/*** sensitivity rate,specificity rate,positive predictive value,negative predictive value and correct indices were 99.30%,98.08%,95.31%,99.79%,and 0.9755,*** this assay,we demonstrate that AMR genes are widely spread,with positive detection rates ranging from 0 to 97.07%in 273 broiler fecal *** CTX-M,bla TEM,mcr-1,fex A,cfr,optr A,and int I1 showed over 80%*** dissemination of AMR genes was distinct between the two *** We successfully established a new high-throughput real-time PCR assay applicable to AMR gene surveillance from fecal *** widespread existence of AMR genes detected in broiler farms highlights the current and severe problem of AMR.

关键词： Antimicrobial resistance Antimicrobial resistance gene High-throughput real-time PCR array Broiler fecal sample

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：