A New High-throughput Real-time PCR Assay for the Screening of Multiple Antimicrobial Resistance Genes in Broiler Fecal Samples from China

作     者：CHE Jie LU Jin Xing LI Wen Ge ZHANG Yun Fei ZHAO Xiao Fei YUAN Min BAI Xue Mei CHEN Xia LI Juan 

作者机构：State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2019年第32卷第12期

页      面：881-892页

核心收录：

学科分类：090601[农学-基础兽医学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 09[农学] 0906[农学-兽医学] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China [Grant agreement 31502124] the National Science and Technology Major Project of China [Grant agreement 2018ZX10733402] 

主　　题：Antimicrobial resistance Antimicrobial resistance gene High-throughput real-time PCR array Broiler fecal sample 

摘      要：Objective Antimicrobial resistance(AMR)has become a global concern and is especially severe in *** effectively and reliably provide AMR data,we developed a new high-throughput real-time PCR assay based on microfluidic dynamic technology,and screened multiple AMR genes in broiler fecal *** A high-throughput real-time PCR system with an new designed integrated fluidic circuit assay were performed AMR gene detection.A total of 273 broiler fecal samples collected from two geographically separated farms were screened AMR *** The new assay with limits of detection ranging from 40.9 to 8,000 copies/*** sensitivity rate,specificity rate,positive predictive value,negative predictive value and correct indices were 99.30%,98.08%,95.31%,99.79%,and 0.9755,*** this assay,we demonstrate that AMR genes are widely spread,with positive detection rates ranging from 0 to 97.07%in 273 broiler fecal *** CTX-M,bla TEM,mcr-1,fex A,cfr,optr A,and int I1 showed over 80%*** dissemination of AMR genes was distinct between the two *** We successfully established a new high-throughput real-time PCR assay applicable to AMR gene surveillance from fecal *** widespread existence of AMR genes detected in broiler farms highlights the current and severe problem of AMR.