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Cyclic and Linear Thiopeptides from Soil-Derived Streptomyces *** 203702 with Antiviral and Antibacterial Activities

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Chinese Journal of Chemistry 2021年第12期39卷 3277-3284页

作者： Dewu Zhang Yujia Wang Xinxin Hu Xiaoyu Wang Linzi Li Guowei Gu Bingyuan Zhang Shan Cen Xuefu You Liyan Yu Division for Medicinal microorganisms Related Strains CAMS collection Center of Pathogenic microorganisms Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China College of Health and Environment Beijing Union UniversityBeijing 100023China School of Pharmacy Yantai UniversityYantaiShangdong 264005China

A new cyclic thiopeptide,berninamycin F(3),three new linear thiopeptides,berninamycins G—I(4-6),and two known berninamycin derivatives,berninamycins C and D(1 and 2)were isolated from Streptomyces *** *** structures were elucidated through HRESIMS and one-and two-dimensional NMR data,and the absolute configurations were assigned using Marfey's *** 4-6 are the first examples of linear berninamycin ***,compound 4 is the first example of linear thiopeptide with a 1-(oxazol-2-yl)ethan-1-one group,compound 6 is the first linear thiopeptide derivative with methylated oxoacetate *** 1-6 exhibited excellent anti-Zika virus activities with IC_(50) values in the range of 4.4-10.5μmol/L,which were superior to that of the positive control ribavirin(IC_(50)=49.2μmol/L).Compounds 1 and 3 showed strong anti-influenza A virus activities with the IC_(50) values of 15.6 and 3.2μmol/L,*** 1 exhibited good antibacterial activities against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus ***.

关键词： Thiopeptide Streptomyces Natural products Structure elucidation Antiviral agents

The Philippine national collection of microorganisms (PNCM):Repository of Microbial Diversity of the Country

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Journal of Ocean University of Qingdao 2002年第1期1卷 101-104页

作者： Monsalud R.G. Magbanua F.O. Parungao M.P. Banaay C.G.B. Bayer M.H.D. Yap J.K. Tapay L.M. Philippine national collection of microorganisms Philippine National Collection of Microorganisms Philippine National Collection of Microorganisms Philippine National Collection of Microorganisms Philippine National Collection of Microorganisms Philippine National Collection of Microorganisms Philippine National Collection of Microorganisms National Institute of Biotechnology & Applied Microbiology University of the Philippines Los Baos College Laguna 4031 Philippines National Institute of Biotechnology & Applied Microbiology University of the Philippines Los Baos College Laguna 4031 Philippines National Institute of Biotechnology & Applied Microbiology University of the Philippines Los Baos College Laguna 4031 Philippines National Institute of Biotechnology & Applied Microbiology University of the Philippines Los Baos College Laguna 4031 Philippines National Institute of Biotechnology & Applied Microbiology University of the Philippines Los Baos College Laguna 4031 Philippines National Institute of Biotechnology & Applied Microbiology University of the Philippines Los Baos College Laguna 4031 Philippines National Institute of Biotechnology & Applied Microbiology University of the Philippines Los Baos College Laguna 4031 Philippines

The prime function of the Philippine national collection of microorganisms (PNCM), being the national repository of microbial strains, is to collect and preserve strains for their continued viability and availability for future use. To date, a total of 2144 strains of bacteria (1357), yeasts (250), filamentous fungi (377), algae (14), and strains still to be identified (146) are maintained at the PNCM. These are preserved and maintained using various methods which include modified liquid drying (lyophilization), ultra-low temperature (-70℃) storage in 10% glycerol, storage in sterile soil, distilled water and overlaying with mineral oil. Periodic viability testing is done to assess the stability of these preserved cultures under storage. Aside from preservation and maintenance of cultures, the PNCM is also involved in several research activities. One of these is the isolation, characterization and identification of some Vibrio isolates from the Philippines. Details on this particular study is presented in this report.

关键词：收藏文化菲律宾人微生物多样性细菌

Biodegradation of Styrene-Butadiene-Styrene Coploymer via Sugars Attached to the Polymer Chain

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Advances in Materials Physics and Chemistry 2013年第2期3卷 112-118页

作者： Rakesh Singh Rishi Gupta Mukund G. Adsul Ramesh C. Kuhad Digambar V. Gokhale Anjani J. Varma Department of Microbiology University of Delhi (South Campus) New Delhi India national collection of industrial microorganisms Biochemical Sciences Division National Chemical Laboratory Pune India Polymer Science & Engineering Division National Chemical Laboratory Pune India

A synthetic method was developed to chemically attach few molecules of simple sugars like glucose, mannose, galactose, maltose and xylose (0.09 - 0.37 wt%) and with quaternary nitrogen pendants (0.42 - 0.46 atomic%) along the polybutadiene section of polystyrene-block-polybutadiene-block-polystyrene (SBS) block copolymer. These functionalized SBS copolymers were evaluated for biodegradation using the fungal culture Aspergillus niger NCIM 1025 (ATCC 9642) and bacterial culture Pseudomonas sp. NCIM 2220, and for antimicrobial properties using bacteria E. coli DH5α and Bacillus subtilis and yeasts Pichia stipitis NCIM 3497 and P. stipitis NCIM 3499. It was conclusively demonstrated that these modified SBS block copolymers were significantly more biodegradable than the unmodified SBS;the observed weight loss after biodegradation was ~4 - 14-fold for bacterial and ~7 - 36-fold for fungal cultures with respect to the sugar content of modified SBS. Preliminary studies on antimicrobial properties of these biodegradable polymers showed a 4% - 24% decrease in growth of the microorganisms E. coli and Bacillus subtilis studied.

关键词： Biodegradation Antimicrobial SBS Sugar Quaternary Nitrogen

Panoramic Insights into Microevolution and Macroevolution of A Prevotella copri-containing Lineage in Primate Guts

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Genomics, Proteomics & Bioinformatics 2022年第2期20卷 334-349页

作者： Hao Li Jan P.Meier-Kolthoff Canxin Hu Zhongjie Wang Jun Zhu Wei Zheng Yun Tian Feng Guo School of Life Sciences Xiamen UniversityXiamen 361102China Department of Bioinformatics Leibniz Institute DSMZ–German Collection of Microorganisms and Cell CulturesD-38124 BraunschweigGermany Fujian Provincial Universities Key Laboratory of Microbial Resource Xiamen UniversityXiamen 361102China

Prevotella copri and its related taxa are widely detected in mammalian gut microbiomes and have been linked with an enterotype in ***,their microevolution and macroevolution among hosts are poorly *** this study,extensively collected marker genes and genomes were analyzed to trace their evolutionary history,host specificity,and biogeographic *** based on marker genes and genomes suggest that a ***-containing lineage(PCL)harbors diverse species in higher ***,*** in the human gut consisted of multiple groups exhibiting high genomic divergence and conspicuous but non-strict biogeographic *** African strains with high genomic divergence from other strains were phylogenetically located at the root of the species,indicating the co-evolutionary history of *** and Homo ***,although long-term co-evolution between PCL and higher primates was revealed,sporadic signals of co-speciation and extensive host jumping of PCL members were suggested among higher *** and phylogenetic analyses indicated that *** and other PCL species found in domesticated mammals had been recently transmitted from ***,strong evidence was found on the extensively horizontal transfer of genes(e.g.,genes encoding carbohydrate-active enzymes)among sympatric *** groups and PCL species in the same primate *** study provides panoramic insights into the combined effects of vertical and horizontal transmission,as well as potential niche adaptation,on the microevolutionary and macroevolutionary history for an enterotyperepresentative lineage.

关键词： Prevotella copri Co-speciation Gut microbiome Host Biogeography

Occurrence of viable but non-culturable(VBNC)pathogenic bacteria in tap water of public places

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Frontiers of Environmental Science & Engineering 2024年第3期18卷 115-129页

作者： Lizheng Guo Xinyan Xiao Kassim Chabi Yiting Zhang Jingjing Li Su Yao Xin Yu China national Research Institute of Food and Fermentation Industries Co. Ltd.China Center of Industrial Culture CollectionBeijing 100015China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China College of Environment&Ecology Xiamen UniversityXiamen 361102China

Viable but non-culturable(VBNC)bacteria have been detected in source water and effluent of drinking water treatment processes,leading to significant underestimation of viable cell *** information exists on VBNC bacteria in tap water,particularly in public *** address this gap,a comprehensive nine-month study was conducted in a major city in south-eastern China,using culture-based and quantitative PCR with propidium monoazide(PMA)dye ***-five samples were collected from five representative public places(railway station,campus,hospital,shopping mall,and institution).The findings revealed that culturable bacteria represented only 0–17.51%of the viable 16S rRNA genes,suggesting that the majority of viable bacteria existed in an uncultured or VBNC ***,opportunistic pathogens such as Escherichia coli,Enterococcus faecalis,Pseudomonas aeruginosa,Salmonella sp.,and Shigella *** primarily detected as VBNC cells,with concentrations ranging from 1.03×10^(0)to 3.01×10^(3),1.20×10^(0)to 1.42×10^(2),1.32×10^(0)to 8.82×10^(0),1.00×10^(0)to 6.71×10^(1),and 2.07×10^(0)to 1.93×10^(2)cell equivalent/100 mL,*** *** was observed in tap water after prolonged stagnation,indicating potential risks associated with bacterial *** and temporal factors accounted for 17.1%and 26.0%,respectively,of the variation in tap water community structure during the sampling period,as revealed by 16S rRNA amplicon *** study provides quantitative insights into the occurrence of VBNC bacteria in tap water and highlights the need for more sensitive monitoring methods and microbial control techniques to enhance tap water safety in public locations.

关键词： VBNC Pathogenic bacteria PMA treatment Public tap water Community structure

Transfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins in Kluyveromyces marxianus

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mLife 2024年第1期3卷 129-142页

作者： Pingping Wu Wenjuan Mo Tian Tian Kunfeng Song Yilin Lyu Haiyan Ren Jungang Zhou Yao Yu Hong Lu State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghaiChina Shanghai Engineering Research Center of industrial microorganisms ShanghaiChina

Kluyveromyces marxianus is a food-safe yeast with great potential for producing heterologous *** the yield in *** remains a challenge and incorporating large-scale functional modules poses a technical obstacle in *** address these issues,linear and circular yeast artificial chromosomes of ***(KmYACs)were constructed and loaded with disulfide bond formation modules from Pichia pastoris or *** modules contained up to seven genes with a maximum size of 15 *** carried telomeres either from *** or *** were transferred successfully into *** and stably propagated without affecting the normal growth of the host,regardless of the type of telomeres and configurations of *** increased the overall expression levels of disulfide bond formation genes and significantly enhanced the yield of various heterologous *** high-density fermentation,the use of KmYACs resulted in a glucoamylase yield of 16.8 g/l,the highest reported level to date in *** and metabolomic analysis of cells containing KmYACs suggested increased flavin adenine dinucleotide biosynthesis,enhanced flux entering the tricarboxylic acid cycle,and a preferred demand for lysine and arginine as features of cells overexpressing heterologous ***,supplementing lysine or arginine further improved the ***,KmYAC provides a powerful platform for manipulating large modules with enormous potential for industrial applications and fundamental *** the disulfide bond formation module via YACs proves to be an efficient strategy for improving the yield of heterologous proteins,and this strategy may be applied to optimize other microbial cell factories.

关键词： disulfide bond formation expression of heterologous proteins Kluyveromyces marxianus telomere yeast artificial chromosome

A novel ascosporogenous yeast species,Zygosaccharomyces siamensis,and the sugar tolerant yeasts associated with raw honey collected in Thailand

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Fungal Diversity 2012年第1期52卷 123-139页

作者： Sujinan Saksinchai Motofumi Suzuki Panuwan Chantawannakul Moriya O.hkuma Saisamorn Lumyong The Graduate School Chiang Mai UniversityChiang Mai 50200Thailand Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Microbe Division/Japan collection of microorganisms(JCM) RIKEN BioResource CenterWakoSaitama 351-0198Japan

Diversity of yeasts in association with bees and their food sources has been explored during the last *** Thailand,there has been no study of yeast identification in honey and ***,a total of 186 yeast strains were isolated from 37 honey samples of 12 different bee *** the basis of morphological and physiological characteristics,55 representative strains were chosen and identified by sequence analysis of the 26S rDNA D1/D2 domain and the ITS *** data were compared with the published sequences and the results showed the occurrence of 19 ascomycetous and 1 basidiomycetous yeast *** strains of the new species were *** analysis of the 26S rDNA D1/D2 sequence revealed that they were conspecific and most closely related to Zygosaccharomyces *** on the ITS sequence,the new species was clustered with the type"and clearly distinguished from the type!.Sequence analysis of combined ITS-26S rDNA D1/D2 showed similar *** occurrence of these two types,with a divergence of more than 1%in their sequences,and low DNA relatedness among them suggested that members of the typeβcan be regarded as separate *** analysis of the morphological and physiological characteristics was *** formation was observed on acetate agar and Gorodkowa *** new Zygosaccharomyces species differed physiologically from *** in 4 assimilation *** data supports the hypothesis that the new species,Zygosaccharomyces siamensis,is a novel ascosporogenous *** type strain is JCM 16825T(=CBS 12273T)and a description is given here.

关键词： Ascosporogenous yeast Zygosaccharomyces siamensis Sugar tolerant yeasts Raw honey Thailand

Investigation on removal pathways of Di 2-ethyl hexyl phthalate from synthetic municipal wastewater using a submerged membrane bioreactor

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Journal of Environmental Sciences 2015年第11期27卷 37-50页

作者： Mehdi Zolfaghari Patrick Drogui Brahima Seyhi Satinder Kaur Brar Gerardo Buelna Rino Dubé Nouha Klai national Institute of Scientific Research Water Earth and Environment (INRS-ETE) University of Quebec industrial Research Center of Quebec

Highly hydrophobic Di 2-ethyl hexyl phthalate（DEHP） is one of the most prevalent plasticizers in wastewaters. Since its half-life in biological treatment is around 25 days, it can be used as an efficiency indicator of wastewater treatment plant for the removal of hydrophobic emerging contaminants. In this study, the performance of submerged membrane bioreactor was monitored to understand the effect of DEHP on the growth of aerobic microorganisms. The data showed that the chemical oxygen demand（COD）and ammonia concentration were detected below 10 and 1.0 mg/L, respectively for operating conditions of hydraulic retention time（HRT） = 4 and 6 hr, sludge retention time（SRT） = 140 day and sludge concentration between 11.5 and 15.8 g volatile solid（VS）/L. The removal efficiency of DEHP under these conditions was higher and ranged between 91% and 98%. Results also showed that the removal efficiency of DEHP in biological treatment depended on the concentration of sludge, as adsorption is the main mechanism of its removal. For the submerged membrane bioreactor, the pore size is the pivotal factor for DEHP removal, since it determines the amount of soluble microbial products coming out of the process. Highly assimilated microorganisms increase the biodegradation rate, as 74% of inlet DEHP was biodegraded; however, the concentration of DEHP inside sludge was beyond the discharge limit. Understanding the fate of DEHP in membrane bioreactor,which is one of the most promising and futuristic treatment process could provide replacement for conventional processes to satisfy the future stricter regulations on emerging contaminants.

关键词： Di 2-ethyl hexyl phthalate Submerged membrane bioreactor Removal efficiency Soluble microbial products Toxicity

Investigation on Diversity of Endophytic Bacterial Community in Xisha Wild Noni(Morinda citrifolia L.) Seed

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Investigation on Diversity of Endophytic Bacterial Community...