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The anthocyanin formation of purple leaf is associated with the activation of LfiHY5 and LfiMYB75 in crape myrtle

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Horticultural Plant Journal 2024年第5期10卷 1230-1246页

作者： Lu Feng Ping Shen Xiufeng Chi Yang Zhou Jieru Liu Tangren Cheng Jia Wang Qixiang Zhang Ming Cai Huitang Pan State Key Laboratory of Efficient Production of Forest Resources Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China

Purple-leafed plants not only have a higher resistance to biotic and abiotic stresses,but also have higher ornamental *** are vital for leaf color formation,growth and development of purple ***,the molecular mechanism underlying purple leaf formation in Lagerstroemia indica remains *** and transcriptomic analysis of purple-leafed cultivar‘Ebony Embers’and greenleafed cultivar‘Arapahoe’showed that the high expression of anthocyanin structure genes induced hyperaccumulation of cyanidin and pelargonidin derivatives,making the leaves ***5,LfiMYB75 and LfibHLH1 were identified using correlation analysis and weighted gene co-expression network ***‘Arapahoe’‘Ebony Embers’population,LfiHY5 and LfiMYB75 showed significant positive correlation with leaf anthocyanin *** expression of LfiMYB75 and LfiHY5 in tobacco and purple-leafed crape myrtle indicated that the two genes activated anthocyanin *** two-hybrid analysis showed that LfiMYB75 and LfibHLH1 could form a complex that enhanced anthocyanin *** monohybrid and dual-luciferase assays confirmed that LfiHY5 activated the expression of LfiMYB75,to activate the transcription of anthocyanin structural genes LfiCHS and ***,there were three alleles of LfiHY5 in crape myrtle,and the different sequences had different activation effects on *** conclusion,the results showed that LfiHY5 led to upregulate the transcription of LfiMYB75,and LfiMYB75 formed a complex with LfibHLH1,which increased the transcription level of LfiCHS and LfiANS to affect anthocyanin synthesis in crape myrtle.

关键词： Lagerstroemia indica Leaf color Anthocyanin HY5 MYB bHLH

Genome-wide identification and characterization of the PbrATG family in Pyrus bretschneideri and functional analysis of PbrATG1a in response to Botryosphaeria dothidea

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Horticultural Plant Journal 2024年第2期10卷 327-340页

作者： Yun Wang Xun Sun Zhenwu Zhang Zhihua Xie Kaijie Qi Shaoling Zhang center of Pear engineering Technology research State Key Laboratory of Crop Genetics and Germplasm EnhancementCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China

The pear is an economic fruit that is widely planted around the world and is loved by people for its rich nutritional value. Autophagy is a self-protection mechanism in eukaryotes, and its occurrence often accompanied by the degradation of damaged substances in cells and the recycling of nutrients. Autophagy is one of the mechanisms through which plants respond to environmental stress and plays an important role in plant development and stress resistance. Functional studies of autophagy-related genes (ATGs) have been performed on a variety of plant species, but little information is available on the ATG family in pear (Pyrus bretschneideri Rehd). Therefore, we analyzed the evolutionary dynamics and performed a genome-wide characterization of the PbrATG gene family. A total of 28 PbrATG members were *** analysis showed that PbrATGs were more closely related to ATGs of European pear and apple. Evolutionary analysis revealed that whole-genome duplication (WGD) and dispersed duplication events were the main driving forces of PbrATG family *** analysis of different pear tissues showed that all the genes were expressed in different pear tissues, and different PbrATGs are expressed at different times and in different locations. Moreover, all PbrATGs also responded to different abiotic stresses, especially salt and drought stress, which elicited the highest expression levels. Pear seedlings were subsequently infected with Botryosphaeria dothidea (***). The results showed that different PbrATGs had different expression patterns at different infection stages. According to the gene expression data, PbrATG1a was selected as a key autophagy gene for further analysis. Silencing of PbrATG1a reduced the resistance of pear to B. dothidea, which resulted in increased lesions, reactive oxygen species (ROS) contents, antioxidant enzyme activity, and gene expression levels in the silenced pear seedlings after B. dothidea inoculation. In this s

关键词： Autophagy Pear PbrATGs Abiotic stress Botryosphaeria dothidea PbrATG1a

Differences in leaf cuticular wax induced by whole-genome duplication in autotetraploid sour jujube

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Horticultural Plant Journal 2024年第1期10卷 66-76页

作者： Meng Li Chenxing Zhang Lu Hou Xinru Liu Hanqing Zhao Xiaoming Pang Wenhao Bo Yingyue Li national engineering research center of Tree Breeding and Ecological Restoration College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China Key Laboratory of Genetics and Breeding in Forest Trees and ornamental Plants Ministry of EducationCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China The Tree and ornamental Plant Breeding and Biotechnology Laboratory of national Forestry and Grassland Administration Beijing Forestry UniversityBeijing 100083China

Drought-resistant plants exhibit strong water retention *** this regard,the autotetraploid sour jujube leaves exhibit better water retention than diploid *** comparisons and physiological comparisons of diploid and autotetraploid leaves showed that the autotetraploid leaves had thicker leaf cuticles and more leaf wax accumulation than the diploid leaves,which could reduce cuticle permeability and improve the drought tolerance of *** this study,the cuticular wax crystalloids on the adaxial and abaxial sides of young and mature jujube leaves were observed in the two ploidy types,and unique cuticular wax crystalloids covering a large area of the cuticle on autotetraploid sour jujube leaves may provide an advantage in reducing leaf non-stomata transpiration and improving plant drought *** on the transcriptome,115 differentially expressed genes between diploids and autotetraploids were further analyzed and found to be involved in the accumulation of cuticular wax components,including terpenoids,fatty acids,and lipids,as well as ABC transporter and wax biosynthetic ***,14 genes differentially expressed between glossy autotetraploid leaves and nonglossy diploid leaves,such as LOC107414787,LOC107411574 and LOC107413721,were screened as candidate genes by qRT-PCR *** findings provided insights into how polyploidization improved drought tolerance.

关键词： Autotetraploid Jujube Drought tolerance Cuticular wax RNA-seq

Identification,characterization,and verification of miR399 target gene in grape

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Horticultural Plant Journal 2024年第1期10卷 91-102页

作者： Maosong Pei Hainan Liu Tonglu Wei Huiying Jin Yihe Yu Mengting Ma Xiaomeng Song Rundong Dai Dalong Guo Henan engineering Technology research center of Quality Regulation and Controlling of Horticultural Plants College of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China

The microRNA miR399 plays an important role in phosphorus signal transduction pathways in ***,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target *** miR399s were identified by miRNA sequencing and retrieval from the miRBase *** mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target *** cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was *** grape miR399 family comprised nine precursor sequences and nine mature *** precursor sequences formed a typical and stable stem—loop *** minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly *** grape miR399 family was phylogenetically closely related to peach,apple,and citrus *** miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and *** cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’*** GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.

关键词： Grape miR399 Phosphorus metabolism Early ripening

Flavonoid extracts from chrysanthemum with appropriate anthocyanins turn blue when exposed to iron ions

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Horticultural Plant Journal 2024年第3期10卷 837-852页

作者： Yanfei Li Jiaying Wang Chenfei Lu Zhongman Wang Chengyan Deng Kang Gao Jingjing Li Zhijun Fang Hao Liu Yan Hong Silan Dai Beijing Key Laboratory of ornamental Plants Germplasm Innovation&Molecular Breeding National Engineering Research Center for FloricultureBeijingLaboratory of Urban and Rural Ecological EnvironmentKey Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of EducationSchool of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China Beijing Greenland Maintenance and Management Service center Beijing 102211China Beijing North Asia Landscape Co. Beijing 102211China

Although some species that accumulate only cyanidin(Cy)in nature can produce blue flowers through iron ions,there has been no evidence of blue chrysanthemums being generated in this *** study revealed that flavonoid extracts from the ray florets of the chrysanthemum cultivar‘Wandai Fengguang’turned blue when exposed to Fe^(3+).Samples that could turn blue were labeled as CB(Cy-determined blue flowers),while samples that did not turn blue were labeled as CN(Cy-determined non-blue flowers).After a series of experiments,a stable screening system was established using flavonoid extracts containing NaAc buffer at pH 5.5 and a total anthocyanin concentration(TAC)of 30 μmol·L^(-1),and the addition of Fe^(3+)from 0 to 0.25 μmol·L^(-1)allowed for the selection of five CB samples from 39 chrysanthemum *** five CB samples exhibited flower color phenotypes that belonged to Cluster-I with redness(a*)values ranging from 29.03 to 45.99,yellowness(b*)values from-11.31 to 3.77,and brightness(L*)values from 29.07 to ***,the ratio of TAC to total luteolin concentration(TLC)was found to be a critical factor for distinguishing between CB and CN *** realize the desired blue hue in the flavonoid extracts with the participation of Fe^(3+),a TAC to TLC ratio of 2.25 and above is ***,the protoplasts and ray florets of CB samples that turned blue with the involvement of Fe^(2+)showed great potential for cultivating blue chrysanthemums through ferric-anthocyanin ***,this study reveals that blue flowers can be cultivated through the increase in the iron ion concentration,combined with the accumulation of Cy.

关键词： Chrysanthemum×morifolium Cyanidin Copigment Ferric-anthocyanin chelate Blue flower

TrG2P:A transfer-learning-based tool integrating multi-trait data for accurate prediction of crop yield

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Plant Communications 2024年第7期5卷 16-27页

作者： Jinlong Li Dongfeng Zhang Feng Yang Qiusi Zhang Shouhui Pan Xiangyu Zhao Qi Zhang Yanyun Han Jinliang Yang Kaiyi Wang Chunjiang Zhao Information Technology research center Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China national engineering research center for Information Technology in Agriculture Beijing 100097China Department of Agronomy and horticulture University of Nebraska-LincolnLincolnNE 68583USA center for Plant Science Innovation University of Nebraska-LincolnLincolnNE 68583USA These authors contributed equally to this article

Yield prediction is the primary goal of genomic selection(GS)-assisted crop *** yield is a complex quantitative trait,making predictions from genotypic data is *** learning can produce an effective model for a target task by leveraging knowledge from a different,but related,source domain and is considered a great potential method for improving yield prediction by integrating multi-trait ***,it has not previously been applied to genotype-to-phenotype prediction owing to the lack of an efficient implementation *** therefore developed TrG2P,a transfer-learning-based ***2P first employs convolutional neural networks(CNN)to train models using non-yield-trait phenotypic and genotypic data,thus obtaining pre-trained ***,the convolutional layer parameters from these pre-trained models are transferred to the yield prediction task,and the fully connected layers are retrained,thus obtaining fine-tuned ***,the convolutional layer and the first fully connected layer of the fine-tuned models are fused,and the last fully connected layer is trained to enhance prediction *** applied TrG2P to five sets of genotypic and phenotypic data from maize(Zea mays),rice(Oryza sativa),and wheat(Triticum aestivum)and compared its model precision to that of seven other popular GS tools:ridge regression best linear unbiased prediction(rrBLUP),random forest,support vector regression,light gradient boosting machine(LightGBM),CNN,DeepGS,and deep neural network for genomic prediction(DNNGP).TrG2P improved the accuracy of yield prediction by 39.9%,6.8%,and 1.8%in rice,maize,and wheat,respectively,compared with predictions generated by the best-performing comparison *** work therefore demonstrates that transfer learning is an effective strategy for improving yield prediction by integrating information from non-yield-trait *** attribute its enhanced prediction accuracy to the valuable information ava

关键词： crop genotype to phenotype transfer learning yield prediction multi-trait

Synthesis and Characterization of Phenyl Camellia oleifera Seed Oil Ester Plasticizing PVC

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Journal of Renewable Materials 2024年第3期12卷 615-628页

作者： Wenqing Xiao Yuhang Liu Yuxin He Qiaoguang Li Yongquan Li College of Chemistry and Chemical engineering Zhongkai University of Agriculture and EngineeringGuangzhou510225China College of Chemical engineering Zhejiang University of TechnologyHangzhou310000China Key Laboratory of Germplasm Platform and ornamental Plants College of Horticulture and Landscape ArchitectureZhongkai University of Agriculture and EngineeringGuangzhou510225China

Plasticizers are essential additives in the processing of polyvinyl chloride(PVC),with phthalate plasticizers being widely ***,these conventional plasticizers have been shown to be harmful to human health and environmentally unfriendly,necessitating the exploration of eco-friendly bio-based *** this study,Camellia oleifera seed oil,a specialty resource in China,was utilized as a raw material and reacted with 4,4′-Methylenebis(N,N-diglycidylaniline)(AG-80)to synthesize Phenyl Camellia seed Oil Ester(PCSOE).PCSOE was employed as a plasticizer to prepare modified PVC films with varying concentrations,with the conventional plasticizer dioctyl phthalate(DOP)serving as a *** results demonstrate that PSCOE-plasticized PVC films exhibit enhanced hydrophilicity,tensile strength,and thermal stability compared to DOP-modified PVC *** contact angle of PSCOE-plasticized PVC films ranges from 66.26°to 78.48°,which is generally lower than the contact angle of DOP-modified PVC films at 78.40°,indicating improved hydrophilicity due to the modification with *** tensile strength of PSCOE-plasticized PVC films ranges from 17.73 to 20.17 MPa,all surpassing the value of 16.41 MPa for DOP-modified PVC ***,the temperatures corresponding to 5%,10%,and 50%weight loss for PVC samples modified with PCSOE are higher than those for ***,PCSOE presents a viable alternative to DOP as a plasticizer for PVC materials.

关键词： Polyvinyl chloride(PVC) Camellia oleifera seed oil 4,4′-Methylenebis(N,N-diglycidylaniline) bio-based plasticizer

Transcriptomic Analysis Reveals the Formation Mechanism of Anthocyanins Light-Independent Synthesis in Chrysanthemum

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1599-1621页

作者： Fangye Liu Jiaping Qu Yajun Li Jiawei Fan Yumeng Cui Jingwen Wu Xueqi Gu Xia Li He Huang Beijing Advanced Innovation center for Tree Breeding by Molecular Design Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Education MinistrySchool of Landscape ArchitectureBeijing Forestry UniversityBeijing100083China Urban Construction School Beijing City UniversityBeijing100083China

Chrysanthemum×morifolium is a horticultural crop which plays a vital role in theﬂower industry with signiﬁ-cant economic value and has a cultivation history of over three thousand years in *** accumulation of anthocyanins is always affected by ***,we revealed that anthocyanin accumulation is highly dependent on light in‘2021135’genotype chrysanthemum,while it is light-independent in‘2001402’genotype ***,no literature has been reported regarding the non-photosensitive chrysanthemum in anthocya-nins light-independent synthesis *** the phenotype analysis of 44 F1 generations,we found that light-independence is a dominant trait which can be stable inherited by *** transcriptome of the rayﬂorets of‘2021135’and‘2001402’under light and bagging treatment were sequenced and *** on weighted gene co-expression network analysis(WGCNA),K-means analysis,and Real-Time Quantitative Poly-merase Chain Reaction(RT-qPCR)analysis,16 genes were highly correlated with the anthocyanin *** anthocyanin content of rayﬂorets treated with different light-quality conditions indicated that blue light signiﬁ-cantly affected anthocyanin *** Yeast one-hybrid analysis,CmBIC1.1 and CmBIC1.2 can directly regulate the anthocyanin structural gene *** our study,we revealed the important characteristics of light-independent anthocyanin synthesis in chrysanthemums and screened regulatory factors in light-depen-dent and light-independent anthocyanin synthesis *** results laid the groundwork for subsequent ana-lysis of the molecular mechanism involved in the light-independent synthesis of anthocyanins in chrysanthemums.

关键词： Chrysanthemum non-photosensitive transcriptome anthocyanin biosynthesis

Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple

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Horticultural Plant Journal 2024年第3期10卷 629-640页

作者： Qinglong Dong Yi Tian Xuemei Zhang Dingyue Duan He Zhang Kaiyu Yang Peng Jia Haoan Luan Suping Guo Guohui Qi Ke Mao Fengwang Ma College of Forestry Hebei Agricultural UniversityBaodingHebei 071001China national engineering research center for Agriculture in Northern Mountainous Areas Agricultural Technology Innovation Center in Mountainous Areas of Hebei ProvinceHebei Agricultural UniversityBaodingHebei 071001China State Key Laboratory of Crop Stress Biology for Arid Areas Shaanxi Key Laboratory of AppleCollege of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China College of horticulture Hebei Agricultural UniversityBaodingHebei 071001China

Abiotic stress reduces plant yield and *** transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain ***,we functionally characterized the apple GroupⅢWRKY gene ***-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic *** activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic *** localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal *** analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic *** affinity purification sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene *** binding was further verified by an electrophoretic mobility shift ***,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple.

关键词： Apple MdWRKY115 Osmotic stress Drought stress Function analysis