The anthocyanin formation of purple leaf is associated with the activation of LfiHY5 and LfiMYB75 in crape myrtle

作     者：Lu Feng Ping Shen Xiufeng Chi Yang Zhou Jieru Liu Tangren Cheng Jia Wang Qixiang Zhang Ming Cai Huitang Pan Lu Feng;Ping Shen;Xiufeng Chi;Yang Zhou;Jieru Liu;Tangren Cheng;Jia Wang;Qixiang Zhang;Ming Cai;Huitang Pan

作者机构：State Key Laboratory of Efficient Production of Forest ResourcesBeijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education and College of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2024年第10卷第5期

页      面：1230-1246页

核心收录：

学科分类：090706[农学-园林植物与观赏园艺] 0907[农学-林学] 09[农学] 

基　　金：the National Key R and D Program of China(Grant Nos.2019YFD1000402,2019YFD1001004) the World-Class Discipline Construction and Characteristic Development Guidance Funds for Beijing Forestry University(Grant No.2019XKJS0323) 

主　　题：Lagerstroemia indica Leaf color Anthocyanin HY5 MYB bHLH 

摘      要：Purple-leafed plants not only have a higher resistance to biotic and abiotic stresses,but also have higher ornamental *** are vital for leaf color formation,growth and development of purple ***,the molecular mechanism underlying purple leaf formation in Lagerstroemia indica remains *** and transcriptomic analysis of purple-leafed cultivar‘Ebony Embers’and greenleafed cultivar‘Arapahoe’showed that the high expression of anthocyanin structure genes induced hyperaccumulation of cyanidin and pelargonidin derivatives,making the leaves ***5,LfiMYB75 and LfibHLH1 were identified using correlation analysis and weighted gene co-expression network ***‘Arapahoe’
‘Ebony Embers’population,LfiHY5 and LfiMYB75 showed significant positive correlation with leaf anthocyanin *** expression of LfiMYB75 and LfiHY5 in tobacco and purple-leafed crape myrtle indicated that the two genes activated anthocyanin *** two-hybrid analysis showed that LfiMYB75 and LfibHLH1 could form a complex that enhanced anthocyanin *** monohybrid and dual-luciferase assays confirmed that LfiHY5 activated the expression of LfiMYB75,to activate the transcription of anthocyanin structural genes LfiCHS and ***,there were three alleles of LfiHY5 in crape myrtle,and the different sequences had different activation effects on *** conclusion,the results showed that LfiHY5 led to upregulate the transcription of LfiMYB75,and LfiMYB75 formed a complex with LfibHLH1,which increased the transcription level of LfiCHS and LfiANS to affect anthocyanin synthesis in crape myrtle.