Identification,characterization,and verification of miR399 target gene in grape

作     者：Maosong Pei Hainan Liu Tonglu Wei Huiying Jin Yihe Yu Mengting Ma Xiaomeng Song Rundong Dai Dalong Guo Maosong Pei;Hainan Liu;Tonglu Wei;Huiying Jin;Yihe Yu;Mengting Ma;Xiaomeng Song;Rundong Dai;Dalong Guo

作者机构：Henan Engineering Technology Research Center of Quality Regulation and Controlling of Horticultural PlantsCollege of Horticulture and Plant ProtectionHenan University of Science and TechnologyLuoyangHenan 471023China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2024年第10卷第1期

页      面：91-102页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by Natural Science Foundation of China(Grant No.U1904113) National Key Research and Development Program of China(Grant No.2018YFD1000105) Program for Innovative Research Team(in Science and Technology)in University of Henan Province(Grant No.21IRTSTHN021) Program for Science&Technology Innovation Talents in Universities of Henan Province(Grant No.21HASTIT035) Scientific and technological breakthroughs in Henan Province(Grant No.222102110083) PhD Research Startup Foundation of Henan University of Science and Technology(Grant Nos.13480067,13480068) 

主　　题：Grape miR399 Phosphorus metabolism Early ripening 

摘      要：The microRNA miR399 plays an important role in phosphorus signal transduction pathways in ***,miR399 was shown to be closely associated with berry ripening in grape(Vitis vinifera).The objective of the present study was to elucidate the evolutionary characteristics of the miR399 gene family in grape and to verify the cleavage effect on the target *** miR399s were identified by miRNA sequencing and retrieval from the miRBase *** mature sequences and precursor sequences were subjected to phylogenetic analysis to reconstruct evolutionary trees,as well as secondary structure analysis of the precursor sequence,and prediction of target *** cisacting elements in the miR399 promoter were predicted and the cleavage effect of grape miR399b on its target genes was *** grape miR399 family comprised nine precursor sequences and nine mature *** precursor sequences formed a typical and stable stem—loop *** minimum folding free energy ranged from-55.70 kcal·mol^(-1)to-37.40 kcal·mol^(-1).Multiple sequence alignment revealed that the miR399 family was highly *** grape miR399 family was phylogenetically closely related to peach,apple,and citrus *** miR399s were predicted to target inorganic phosphate transporter 1—3,phospholipase D delta-like,and *** cleavage effect of grape miR399b on the target genes was verified by means of a dual-luciferase assay and 5’*** GUS staining showed that the promoter activity of miR399b was promoted by GA3treatment.