检索结果-南通市图书馆

Elucidation of the melitidin biosynthesis pathway in pummelo

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2023年第11期65卷 2505-2518页

作者： Shuangqian Shen Shouchuang Wang Chenkun Yang Chao Wang Qianqian Zhou Shen Zhou Ran Zhang Yufei Li Zixuan Wang Liupan Dai Wenjv Peng Yingchen Hao Hao Guo Guangping Cao Xianqing Liu Fan Yao Qiang Xu Alisdair RFernie Jie Luo Sanya nanfan research institute of Hainan University Hainan UniversitySanya 572025China National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory College of Life Science and TechnologyCollege of Biomedicine and HealthHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Horticultural Plant Biology of Ministry of Education Huazhong Agricultural UniversityWuhan 430070China Max Planck institute of Molecular Plant Physiology Potsdam-Golm 14476Germany Centre of Plant Systems Biology and Biotechnology Plovdiv 4000Bulgaria Yazhouwan National Laboratory Sanya 572025China

Specialized plant metabolism is a rich resource of compounds for drug discovery.The acylated flavonoid glycoside melitidin is being developed as an anti-cholesterol statin drug candidate,but its biosynthetic route in plants has not yet been fully characterized.Here,we describe the gene discovery and functional characterization of a new flavonoid gene cluster(UDP-glucuronosyltransferases(Cg UGTs),1,2rhamnosyltransferase(Cg1,2Rha T),acyltransferases(Cg ATs))that is responsible for melitidin biosynthesis in pummelo(Citrus grandis(L.)Osbeck).Population variation analysis indicated that the tailoring of acyltransferases,specific for bitter substrates,mainly determine the natural abundance of melitidin.Moreover,3-hydroxy-3-methylglutaryl-Co A reductase enzyme inhibition assays showed that the product from this metabolic gene cluster,melitidin,may be an effective anti-cholesterol statin drug candidate.Co-expression of these clustered genes in Nicotiana benthamiana resulted in the formation of melitidin,demonstrating the potential for metabolic engineering of melitidin in a heterologous plant system.This study establishes a biosynthetic pathway for melitidin,which provides genetic resources for the breeding and genetic improvement of pummelo aimed at fortifying the content of biologically active metabolites.

关键词： bioactive metabolites biosynthetic pathway flavonoids melitidin natural variation

NET位点决定了大米的口感、蒸煮和营养品质

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2022年第20期67卷 2045-2049,M0003页

作者：李宇飞杨壮杨陈坤刘振寰沈双欠占传松吕媛媛张凤李康施宇恒周骏杰刘贤青房传营 Alisdair RFernie 李洁罗杰 National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China Sanya nanfan research institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya 572025China College of Tropical Crops Hainan UniversityHaikou 570228China Max Planck institute of Molecular Plant Physiology Potsdam-Golm 14476Germany John Innes Centre Norwich Research ParkNorwichNR47UHUK

水稻是重要的粮食作物,为全球一半人口提供热量需求.随着经济发展和生活水平的提高,人们对大米的需求逐渐从“吃饱”转变为“吃好”,美味营养的品种是后者的必要条件.以往的研究大多都集中在提高大米的食味和蒸煮品质上以满足口感需求,... 详细信息

水稻是重要的粮食作物,为全球一半人口提供热量需求.随着经济发展和生活水平的提高,人们对大米的需求逐渐从“吃饱”转变为“吃好”,美味营养的品种是后者的必要条件.以往的研究大多都集中在提高大米的食味和蒸煮品质上以满足口感需求,但改善水稻综合营养的研究仍然是零散的.本研究通过正向遗传学手段采用代谢物全基因组关联分析结合代谢物数量性状位点分析方法,定位到一个调控水稻谷粒多种营养代谢物积累的数量性状位点,并命名为NET.实验证明NET位点可以同时调控大米的食用、蒸煮以及营养品质.结合NET位点的驯化路线以及驯化过程中营养代谢物的变化,发现水稻育种过程中食味品质和营养品质没有得到有效的平衡.因此,为满足“吃好”的目标,有必要考虑到育种过程中营养物质的变化,达到两全其美,而NET位点可能是实现这一目标的重要的遗传位点之一.该研究结果阐明了水稻驯化过程中营养代谢物变化的遗传机制,为获得理想的高食用品质、高营养品种提供了可能的育种方案.

关键词：数量性状位点育种方案育种过程理想的高营养品质水稻育种蒸煮品质食味

Breeding design in wheat by combining the QTL information in a GWAS panel with a general genetic map and computer simulation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2023年第6期11卷 1816-1827页

作者： Xiaobo Wang Weiwei Mao Yongfa Wang Hongyao Lou Panfeng Guan Yongming Chen Huiru Peng Jiankang Wang State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Frontiers Science Center for Molecular Design Breeding(MOE) Key Laboratory of Crop Heterosis and UtilizationKey Laboratory of Crop Genetic ImprovementCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Hainan Yazhou Bay Seed Laboratory Sanya 572025HainanChina National nanfan research institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024HainanChina

A large amount of genome-wide association study(GWAS)panels together with quantitative-trait locus(QTL)information associated with breeding-targeted traits have been described in wheat(Triticum aestivum L.).However,the application of mapping results from a GWAS panel to conventional wheat breeding remains a challenge.In this study,we first report a general genetic map which was constructed from 44 published linkage maps.It permits the estimation of genetic distances between any two genetic loci with physical map positions,thereby unifying the linkage relationships between QTL,genes,and genomic markers from multiple genetic populations.Second,we describe QTL mapping in a wheat GWAS panel of 688 accessions,identifying 77 QTL associated with 12 yield and grain-quality traits.Because these QTL have known physical map positions,they could be mapped onto the general map.Finally,we present a design approach to wheat breeding by using known QTL information and computer simulation.Potential crosses between parents in the GWAS panel may be evaluated by the relative frequency of the target genotype,trait correlations in simulated progeny populations,and genetic gain of selected progenies.It is possible to simultaneously improve yield and grain quality by suitable parental selection,progeny population size,and progeny selection scheme.Applying the design approach will allow identifying the most promising crosses and selection schemes in advance of the field experiment,increasing predictability and efficiency in wheat breeding.

关键词： Wheat breeding Breeding design GWAS panel General genetic map Computer simulation

TaBZR1 enhances wheat salt tolerance via promoting ABA biosynthesis and ROS scavenging

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2023年第11期50卷 861-871页

作者： Ruizhen Yang Ziyi Yang Meng Xing Yexing Jing Yunwei Zhang Kewei Zhang Yun Zhou Huixian Zhao Weihua Qiao Jiaqiang Sun State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Life Sciences Northwest A&F UniversityYanglingShaanxi 712100China National nanfan research institute(Sanya) Chinese Academy of Agricultural SciencesSanyaHainan 572024China institute of Plant Genetics and Developmental Biology College of Chemistry and Life SciencesZhejiang Normal UniversityJinhuaZhejiang 321004China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifengHenan 475001China State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&F UniversityYanglingShaanxi 712100China

Brassinosteroids(BRs)are vital plant steroid hormones involved in numerous aspects of plant life including growth,development,and responses to various stresses.However,the underlying mechanisms of how BR regulates abiotic stress responses in wheat(Triticum aestivum L.)remain to be elucidated.Here,we find that BR signal core transcription factor BRASSINAZOLE-RESISTANT1(TaBZR1)is significantly up-regulated by salt treatment.Overexpression of Tabzr1-1D(a gain-of-function TaBZR1 mutant protein)improves wheat salt tolerance.Furthermore,we show that TaBZR1 binds directly to the G-box motif in the promoter of ABA biosynthesis gene TaNCED3 to activate its expression and promotes ABA accumulation.Moreover,TaBZR1 associates with the promoters of ROS-scavenging genes TaGPX2 and TaGPX3 to activate their expression.Taken together,our results elucidate that TaBZR1 improves salt-stress tolerance by activating some genes involved in the biosynthesis of ABA and ROS scavenging in wheat,which gives us a new strategy to improve the salt tolerance of wheat.

关键词： Wheat TaBZR1 Salt stress ABA ROS

Developing an efficient CRISPR-dCas9-TVderived transcriptional activation system to create three novel cotton germplasm materials

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Communications 2023年第4期4卷 5-7页

作者： Lu Yu Zhanshuai Li Xiao Ding Muna Alariqi Chaojun Zhang Xiangqian Zhu Shuli Fan Longfu Zhu Xianlong Zhang Shuangxia Jin State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural ScienceAnyang 455000China Hubei Hongshan Laboratory Wuhan 430070China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China National nanfan research institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China

Dear Editor,With the development of CRISPR-Cas technology,an RNAguided CRISPR activation system has been developed in plants,in which a dead Cas9(dCas9)nuclease is fused to transcriptional activators to regulate the transcription of endogenous genes(Li et al.,2017;Liu et al.,2017;Manghwar et al.,2020;Ming et al.,2020;Pan et al.,2021).Precise upregulation of gene transcription is emerging as a promising approach for functional genomics research,molecular breeding,and germplasm innovation.However,there have been no reports on the use of the CRISPR-dCas9 transcriptional activation system in cotton.

关键词： dCas9 transcriptional CRISPR

The U-box ubiquitin ligase TUD1 promotes brassinosteroid-induced GSK2 degradation in rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Communications 2023年第2期4卷 107-118页

作者： Dapu Liu Xiaoxing Zhang Qingliang Li Yunhua Xiao Guoxia Zhang Wenchao Yin Mei Niu Wenjing Meng Nana Dong Jihong Liu Yanzhao Yang Qi Xie Chengcai Chu Hongning Tong National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China National nanfan research institute Chinese Academy of Agricultural SciencesSanya 572024China

Brassinosteroids(BRs)are a class of steroid hormones with great potential for use in crop improvement.De-repression is usually one of the key events in hormone signaling.However,how the stability of GSK2,the central negative regulator of BR signaling in rice(Oryza sativa),is regulated by BRs remains elusive.Here,we identify the U-box ubiquitin ligase TUD1 as a GSK2-interacting protein by yeast two-hybrid screening.We show that TUD1 is able to directly interact with GSK2 and ubiquitinate the protein.Phenotypes of the tud1 mutant are highly similar to those of plants with constitutively activated GSK2.Consistent with this finding,GSK2 protein accumulates in the tud1 mutant compared with the wild type.In addition,inhibition of BR synthesis promotes GSK2 accumulation and suppresses TUD1 stability.By contrast,BRs can induce GSK2 degradation but promote TUD1 accumulation.Furthermore,the GSK2 degradation process is largely impaired in tud1 in response to BR.In conclusion,our study demonstrates the role of TUD1 in BR-induced GSK2 degradation,thereby advancing our understanding of a critical step in the BR signaling pathway of rice.

关键词： brassinosteroid TUD1 GSK2 signal transduction ubiquitination

The telomere-to-telomere genome of Fragaria vesca reveals the genomic evolution of Fragaria and the origin of cultivated octoploid strawberry

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture research 2023年第4期10卷 101-109页

作者： Yuhan Zhou Jinsong Xiong Ziqiang Shu Chao Dong Tingting Gu Pengchuan Sun Shuang He Mian Jiang Zhiqiang Xia Jiayu Xue Wasi Ullah Khan Fei Chen Zong-Ming Cheng College of Horticulture Nanjing Agricultural UniversityNanjing 210095China Hainan Yazhou Bay Seed Laboratory Sanya 572024China Wuhan Benagen Tech Solutions Company Limited WuhanHubei 430021China Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education&State Key Laboratory of Hydraulics&Mountain River Engineering College of Life SciencesSichuan UniversityChengdu 610065China College of Tropical Crops Hainan UniversityHaikou 570228China Sanya nanfan research institute from Hainan University Sanya 572025China

Fragaria vesca,commonly known as wild or woodland strawberry,is the most widely distributed diploid Fragaria species and is native to Europe and Asia.Because of its small plant size,low heterozygosity,and relative ease of genetic transformation,F.vesca has been a model plant for fruit research since the publication of its Illumina-based genome in 2011.However,its genomic contribution to octoploid cultivated strawberry remains a long-standing question.Here,we de novo assembled and annotated a telomere-to-telomere,gap-free genome of F.vesca‘Hawaii 4’,with all seven chromosomes assembled into single contigs,providing the highest completeness and assembly quality to date.The gap-free genome is 220785082 bp in length and encodes 36173 protein-coding gene models,including 1153 newly annotated genes.All 14 telomeres and seven centromeres were annotated within the seven chromosomes.Among the three previously recognized wild diploid strawberry ancestors,F.vesca,F.iinumae,and F.viridis,phylogenomic analysis showed that F.vesca and F.viridis are the ancestors of the cultivated octoploid strawberry F.×ananassa,and F.vesca is its closest relative.Three subgenomes of F.×ananassa belong to the F.vesca group,and one is sister to F.viridis.We anticipate that this high-quality,telomere-to-telomere,gap-free F.vesca genome,combined with our phylogenomic inference of the origin of cultivated strawberry,will provide insight into the genomic evolution of Fragaria and facilitate strawberry genetics and molecular breeding.

关键词： straw Fragaria origin

De novo assembly of plant complete genomes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tropical Plants 2022年第1期1卷 54-61页

作者： Yuhan Zhou Ji Zhang Xianghui Xiong Zong-Ming Cheng Fei Chen Hainan Yazhou Bay Seed Laboratory&Sanya nanfan research institute from Hainan University Sanya 572025China College of Horticulture Nanjing Agricultural UniversityNanjing 210095China College of Tropical Crops Hainan UniversityHaikou 570228China

Plant genomes encode the mysteries of how plants cope with complex environments over long evolutionary histories.Over the past 20 years,rapidly developing technologies have allowed the decoding of hundreds of plant draft or reference genomes.The diversity,polyploidy and heterozygosity of plants make it technically challenging and time-consuming to generate high-quality plant genome assemblies.Recently invented ultra-long read sequencing technologies have achieved a milestone where several plant genomes have been gapless and assembled into telomere to telomere.Telomere-to-telomere(T2T)genome refers to a high-quality complete genome with high genomic accuracy,high continuity,and high integrity.With the release of the completed human genome and Arabidopsis thaliana genome,the era of complete T2T species genome has arrived.In this review,we summarize the history leading up to the gap free plant genomes based on emerging ultra-long read sequencing technologies.We discuss to close gaps relying on targeted genome sequencing and assembling technologies.However,there are still quite a lot of challenges in super large,polyploidy,and unstable genomes.Nevertheless,these complete genomes have already provided unprecedented information,which will certainly deepen our understanding of plant genomes and the exploration of more functional sequences.By taking advantage of the complete genomes,a series of important genes could be annotated,which will help achieve the goal of genome design in crop species.

关键词： hundreds consuming unstable

Chromosomal-level genome of macadamia(Macadamia integrifolia)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tropical Plants 2022年第1期1卷 13-21页

作者： Chengcai Xia Sirong Jiang Qiujin Tan Wenquan Wang Long Zhao Chenji Zhang Yuting Bao Qi Liu Jianjia Xiao Ke Deng Miaohua He Pengliang An Wenlin Wang Meiling Zou Zhiqiang Xia Sanya nanfan research institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya 572025China College of Tropical Crops Hainan UniversityHaikou 570288China Guangxi South Subtropical Agricultural Science research institute Longzhou 532415China Academy of Agriculture and Forestry Sciences Qinghai UniversityXining 810016China College of Agriculture China Agricultural UniversityBeijing 100083China

Macadamia from the family Proteaceae is a plant native to Australia and has long been favoured by people for its crispy and high nutritional and medicinal value.Here,the genome of GUIRE 1(GR1),a highly heterozygous superior cultivar of macadamia nut,was sequenced and assembled using nanopore sequencing,and a 807-Mb genome(contig N50,1.9 Mb;scaffold N50,54.70 Mb)and 14 chromosomes were obtained.A total of 453 Mb(about 55.95%)repetitive sequences and 37,657 protein-coding genes were obtained by gene annotation and homologous protein comparison.Proteaceae diverged from Nelumbonaceae nearly 115.37 million years ago and from Rubiaceae about 140 million years ago.A genome-wide duplication(WGD)event occurred in macadamia 41 million years ago based on the WGD analysis.The functional enrichment analysis of M.integrifolia-specific gene families revealed their roles in signal transduction,protein phosphorylation,protein binding,and defense response.Here,a highly heterozygous genome of M.integrifolia was unlocked to provide a database for breeding and molecular mechanism research.

关键词： breeding analysis. integrifolia