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Journal of Integrative Plant Biology 2021年第7期63卷 1353-1366页

作者： Zengxiu Feng Hongyong Shi Minghui Lv Yuang Ma Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Brassinosteroids(BRs)are a group of steroidal phytohormones,playing critical roles in almost all physiological aspects during the life span of a plant.In Arabidopsis,BRs are perceived at the cell surface,triggering a reversible phosphorylation-based signaling cascade that leads to the activation and nuclear accumulation of a family of transcription factors,represented by BES1 and BZR1.Protein famesylation is a type of post-translational modification,functioning in many important cellular processes.Previous studies demonstrated a role of famesylation in BR biosynthesis via regulating the endoplasmic reticulum localization of a key bassino-lide(BL)biosynthetic enzyme BR6ox2.Whether such a process is also involved in BR signaling is not understood.Here,we demonstrate that protein famesylation is involved in mediating BR signaling in Arabidopsis.A loss-of-function mutant of EN HANCED RESPONSE TO ABA 1(iERA1).encoding a(3 subunit of the protein famesyl transferase hoi oenzyme,can alter the BL sensitivity of bak1~4 from a reduced to a hypersensitive level,era 7 can partially rescue the BR defective phenotype of a hetero zygous mutant of bin2-1,a gain-of function mutant of BIN2 which encodes a negative regulator in the BR signaling.Our genetic and biochemical analyses revealed that ERA1 plays a significant role in regulating the protein stability of BES1.

关键词： Arabidopsis BES1 brassinolide brassinosteroids protein famesylation

Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis

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Molecular Plant 2021年第7期14卷 1119-1134页

作者： Yafen Zhu Chong Hu Yanwei Cui Li Zeng Sunjingnan Li Mingsong Zhu Fanhui Meng Shuting Huang Li Long Jing Yi Jia Li Xiaoping Gou Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

The shoot apical meristem(SAM)and root apical meristem(RAM)act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants,respectively.The CLAVATA3(CLV3)-WUSCHEL(WUS)negative-feedback loop acts as a core pathway controlling SAM homeostasis,while CLV3/EMBRYO SURROUNDING REGION(ESR)40(CLE40)and WUSCHEL-RELATED HOMEOBOX5(WOX5),homologs of CLV3 and WUS,direct columella stem cell fate.Moreover,CLV3 INSENSITIVE KINASES(CIKs)have been shown to be essential for maintaining SAM homeostasis,whereas whether they regulate the distal root meristem remains to be elucidated.Here,we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root meristem.We found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell(CSC)differentiation.Biochemical assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4(ACR4)in a ligand-independent manner and can be phosphorylated by ACR4 in vitro.In addition,the phosphorylation of CIKs can be rapidly induced by CLE40,which partially depends on ACR4.Although CIKs act as conserved and redundant regulators in the SAM and RAM,our results demonstrated that they exhibit differentiated functions in these meristems.

关键词： ACR4 CIKs CLE40 stem cells distal root meristem phosphorylation

Receptor-like protein kinase-mediated signaling in controlling root meristem homeostasis

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aBIOTECH 2020年第3期1卷 157-168页

作者： Yafen Zhu Chong Hu Xiaoping Gou Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Generation of the root greatly benefits higher plants living on land.Continuous root growth and development are achieved by the root apical meristem,which acts as a reservoir of stem cells.The stem cells,on the one hand,constantly renew themselves through cell division.On the other hand,they differentiate into functional cells to form diverse tissues of the root.The balance between the maintenance and consumption of the root apical meristem is governed by cell-to-cell communications.Receptor-like protein kinases(RLKs),a group of signaling molecules localized on the cell surface,have been implicated in sensing multiple endogenous and environmental signals for plant development and stress adaptation.Over the past two decades,various RLKs and their ligands have been revealed to participate in regulating root meristem homeostasis.In this review,we focus on the recent studies about RLK-mediated signaling in regulating the maintenance and consumption of the root apical meristem.

关键词： Distal root meristem Peptides Proximal root meristem Receptor-like protein kinases Root apical meristem Signal transduction

Receptor-like Kinases in Root Development:Current Progress and Future Directions

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Molecular Plant 2021年第1期14卷 166-185页

作者： Yang Ou Hong Kui Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

cell-to-cell and cell-to-environment communications are critical to the growth and development of plants.cell surface-localized receptor-like kinases(RLKs)are mainly involved in sensing various extracellular signals to initiate their corresponding cellular responses.As important vegetative organs for higher plants to adapt to a terrestrial living situation,roots play a critical role for the survival of plants.It has been demonstrated that RLKs control many biological processes during root growth and development.In this review,we summarize several key regulatory processes during Arabidopsis root developm ent in which RLKs play critical roles.We also put forward a number of relevant questions that are required to be explored in future studies.

关键词： receptor-like kinase root development Arabidopsis

RGF1-RGI1,a Peptide-Receptor Complex,Regulates Arabidopsis Root Meristem Development via a MAPK Signaling Cascade

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Molecular Plant 2020年第11期13卷 1594-1607页

作者： Xiaoting Lu Hongyong Shi Yang Ou Yanwei Cui Jinke Chang Liang Peng Xiaoping Gou Kai He Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Root growth is maintained by the continuous division of cells in the apical meristem.ROOT MERISTEM GROWTH FACTOR 1(RGF1)is a critical peptide hormone regulating root stem cell niche maintenance.Previous studies discovered that five closely related leucine-rich repeat receptor-like protein kinases(LRRRLKs),named RGF1 INSENSITIVES(RGIs)or RGF1 RECEPTORS(RGFRs),are able to perceive the RGF1 signal and redundantly control root stem cell niche maintenance.RGF1 regulates root meristem activity mainly via two downstream transcription factors,PLETHORA 1(PLT1)and PLT2.Regulatory proteins connecting cell surface RGF1-RGI1 and nuclear PLTs,however,were not identified.Here,we report that the mitogen-activated protein(MAP)kinase kinase 4(MKK4)and MAP kinase 3(MPK3)were coimmunoprecipitated with RGI1-FLAG after Arabidopsis seedlings were treated with RGF1.Genetic and biochemical assays confirmed that MKK4 and MKK5,and their downstream targets MPK3 and MPK6,are essential RG卜dependent regulators of root meristem development.In addition,we found that the MKK4/MKK5-MPK3/MPK6 module functions downstream of YDA,a MAPKKK.Our results demonstrate that RGF1-RGI1 regulate the expression of PLT1/PLT2 via a YDA-MKK4/MKK5-MPK3/MPK6 signaling cascade.

关键词： root meristem RGF1 RGI1 RLK MAPK

TCP1 Modulates DWF4 Expression via Directly Interacting with the GGNCCC Motifs in the Promoter Region of DWF4 in Arabidopsis thaliana

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Journal of Genetics and Genomics 2015年第7期42卷 383-392页

作者： Yahu Gao Dongzhi Zhang Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou University

Our previous studies indicated that TCP1 is a positive regulator of the brassinosteroid （BR） biosynthesis pathway by mediating the transcription of DWF4, one of the key BR biosynthetic genes in Arabidopsis thaliana. Whether TCP1 can directly bind to the promoter region of DWF4, however, has not been experimentally demonstrated. Here we provide our biochemical and genetic evidence that TCP1 mediates the expression of DWF4 by directly associating with the two GGNCCC motifs in the promoter region of DWF4. The expression levels of DWF4 are positively correlated to TCP1 abundance in planta. Electrophoretic mobility shift assays （EMSAs） using various synthetic DNA fragments suggest that the GGNCCC core sequence is critical for TCP1 binding. DNA sequences flanking the GGNCCC motifs are less important for the association of TCPI. Using DWF4p-GUS transgenic plants as an assay system, it is clearly indicated that these motifs are required for the positive regulation of DWF4 transcription by TCP1. More significantly, whole genome microarray analyses indicate that TCP1 can directly or indirectly regulate the expression of many other genes known to be important for normal plant growth and development.

关键词： Brassinosteroids TCP1 DWF4 Arabidopsis Homeostasis

Receptor-like protein kinases:key regulators controlling root hair development in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2018年第9期60卷 841-850页

作者： Zhuoyun Wei Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Root hairs are tubular outgrowths specifically differentiated from epidermal cells in a differentiation zone. The formation of root hairs greatly increases the surface area of a root and maximizes its ability to absorb water and inorganic nutrients essential for plant growth and development. Root hair development is strictly regulated by intracellular and intercellular signal communications. cell surface-localized receptor-like protein kinases （P, LKs） have been shown to be important components in these cellular processes, tn this review, the functions of a number of key P, LKs in regulating Arabidopsis root hair development are discussed, especially those involved in root epidermal cell fate determination and root hair tip growth.

关键词： Receptor-like protein kinases:key regulators controlling root hair development in Arabidopsis thaliana

BAK1 Directly Regulates Brassinosteroid Perception and BRI1 Activation

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Journal of Integrative Plant Biology 2013年第12期55卷 1264-1270页

作者： Kai He Shengbao Xu Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou University

Plants utilize plasma membrane-localized receptor-like kinases （RLKs） to sense extracellular signals to coordinate growth, development, and innate immune responses. BAK1 regulates multiple signaling pathways acting as a co-receptor of several distinct ligand-binding RLKs. It has been debated whether BAK1 serves as an essential regulatory component or only a signal amplifier without pathway specificity. This issue has been clarified recently. Genetic and structural analyses indicated that BAK1 and its homologs play indispensible roles in mediating brassinosteroid （BR） signaling pathway by directly perceiving the ligand BR and activating the receptor of BR, BRII. The mechanism revealed by these studies now serves as a paradigm for how a pair of RLKs can function together in ligand binding and subsequent initiation of signaling.

关键词： Arabidopsis BAK1 brassinosteroid BRI1 co-receptor extracellular domain leucine-rich repeat receptor receptor-like kinase.

Transmembrane kinase 1-mediated auxin signal regulates membrane-associated clathrin in Arabidopsis roots

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Journal of Integrative Plant Biology 2023年第1期65卷 82-99页

作者： Yutong Wang Xu Yan Mei Xu Weiyang Qi Chunjie Shi Xiaohong Li Jiaqi Ma Dan Tian Jianxin Shou Haijun Wu Jianwei Pan Bo Li Chao Wang Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life Sciences Lanzhou UniversityLanzhou 730000 China College of Life Sciences Shaoxing University Shaoxing 312000 China

Clathrin-mediated endocytosis(CME)is the major endocytic pathway in eukaryotic cells that directly regulates abundance of plasma membrane proteins.Clathrin triskelia are composed of clathrin heavy chains(CHCs)and light chains(CLCs),and the phytohormone auxin differentially regulates membraneassociated CLCs and CHCs,modulating the endocytosis and therefore the distribution of auxin efflux transporter PIN-FORMED2(PIN2).However,the molecular mechanisms by which auxin regulates clathrin are still poorly understood.Transmembrane kinase(TMKs)family proteins are considered to contribute to auxin signaling and plant development;it remains unclear whether they are involved in PIN transport by CME.We assessed TMKs involvement in the regulation of clathrin by auxin,using genetic,pharmacological,and cytological approaches including live-cell imaging and immunofluorescence.In tmk1 mutant seedlings,auxin failed to rapidly regulate abundance of both CHC and CLC and to inhibit PIN2 endocytosis,leading to an impaired asymmetric distribution of PIN2 and therefore auxin.Furthermore,TMK3 and TMK4 were shown not to be involved in regulation of clathrin by auxin.In summary,TMK1 is essential for auxin-regulated clathrin recruitment and CME.TMK1therefore plays a critical role in the establishment of an asymmetric distribution of PIN2 and an auxin gradient during root gravitropism.

关键词： Arabidopsis auxin clathrin endocytosis PIN2 transmembrane kinase1(TMK1)

Three divergent approaches identified the same RGF1 receptors in Arabidopsis thaliana

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Science China(Life Sciences) 2017年第9期60卷 1040-1043页

作者： Yang Ou Jia Li Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life Sciences Lanzhou University Lanzhou 730000 China

During adaptation to terrestrial environment,plants evolved an important vegetative organ,root,to achieve immobilization,absorption of water and nutrients,biosynthesis,transportation and other physiological functions.Stem cell niche in root apical meristem,including quiescent center(QC)cells and their surrounding stem cells(Figure 1),is critical for normal root growth and development.Positions of stem cells within the stem cell niche determine their cell fates.

关键词：拟南芥组织干细胞受体陆地环境营养器官植物进化生理功能固定化