Protein farnesylation negatively regulatesbrassinosteroid signaling via reducing BES1 stabilityinArabidopsis thaliana^(∞)

作     者：Zengxiu Feng Hongyong Shi Minghui Lv Yuang Ma Jia Li Zengxiu Feng;Hongyong Shi;Minghui Lv;Yuang Ma;Jia Li

作者机构：Ministry of Education Key Laboratory of Cell Activities and Stress AdaptationsSchool of Life SciencesLanzhou UniversityLanzhou 730000China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第7期

页      面：1353-1366页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：supported by the National Natural Sci-ence Foundation of China Grants 31720103902 ,32030005(to J.L.) 111 Project B16022(to J.L.) the Funda-mental Research Funds for the Central Universities Grant no.lzujbky‐2020‐sp04(to J.L.),lzujbky‐2021‐kb05。 

主　　题：Arabidopsis BES1 brassinolide brassinosteroids protein famesylation 

摘      要：Brassinosteroids(BRs)are a group of steroidal phytohormones,playing critical roles in almost all physiological aspects during the life span of a plant.In Arabidopsis,BRs are perceived at the cell surface,triggering a reversible phosphorylation-based signaling cascade that leads to the activation and nuclear accumulation of a family of transcription factors,represented by BES1 and BZR1.Protein famesylation is a type of post-translational modification,functioning in many important cellular processes.Previous studies demonstrated a role of famesylation in BR biosynthesis via regulating the endoplasmic reticulum localization of a key bassino-lide(BL)biosynthetic enzyme BR6ox2.Whether such a process is also involved in BR signaling is not un­derstood.Here,we demonstrate that protein fame­sylation is involved in mediating BR signaling in Arabidopsis.A loss-of-function mutant of EN HANCED RESPONSE TO ABA 1(iERA1).encoding a(3 subunit of the protein famesyl transferase hoi oenzyme,can alter the BL sensitivity of bak1~4 from a reduced to a hypersensitive level,era 7 can partially rescue the BR defective phenotype of a hetero zygous mutant of bin2-1,a gain-of function mutant of BIN2 which encodes a negative regulator in the BR signaling.Our genetic and biochemical analyses re­vealed that ERA1 plays a significant role in regulating the protein stability of BES1.