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Lycorine derivative effectively inhibits the replication of coronaviruses both in vitro and in vivo

hLife 2024年第2期2卷 75-87页

作者： Liang Shen Jianzhong Zhao Ying Xia Junjie Lu Jiali Sun Jian Tang Hui Xing Lijuan Yin Yang Yang Chunhua Wang Department of Clinical Laboratory Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceHubeiChina Shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People’s HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyGuangdongChina Department of Clinical Laboratory Xiangyang No.1 People’s HospitalHubei University of MedicineHubeiChina State Key Laboratory of Food Nutrition and Safety Key Laboratory of Industrial MicrobiologyMinistry of EducationTianjin Key Laboratory of Industry MicrobiologyNational and Local United Engineering Lab of Metabolic Control Fermentation TechnologyChina International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal ChemistryCollege of BiotechnologyTianjin University of Science&TechnologyTianjinChina

The established and ongoing prevalence of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and seasonal human coronaviruses(HCoV)like HCoV-OC43,HCoV-NL63,and HCoV-229E,pose a continuous threat to public ***,it is urgently needed to explore antiviral drugs with broad-spectrum anti-coronavirus *** previous studies have revealed that lycorine is a potent broad-spectrum anti-coronavirus drug,a natural alkaloid extracted from Amaryllidaceae with various pharmacological and microbiological ***,it is unsafe to directly use lycorine as a clinical antiviral drug due to the cytotoxicity and induction of cell *** this study,a series of lycorine derivatives were designed and *** of them,named Ly-8,was found to effectively inhibit the replication of different coronavirus strains in vitro,including ***,Ly-8 was also shown to effectively inhibit HCoVOC43 replication in the central nervous system,and provide effective protection against HCoV-OC43 infection in mice with low drug ***,Ly-8-resistant mutants were not observed during the 30 times sequential passages in cell ***,these findings suggest that Ly-8 may be a potential candidate drug for the future development of broad-spectrum anti-coronavirus drugs.

关键词： broad-spectrum antiviral drug lycorine derivative Ly-8

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Impacts of heavy metals on 1,2-dichloroethane biodegradation in co-contaminated soil

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Journal of Environmental Sciences 2009年第5期21卷 661-666页

作者： Ademola O. Olaniran Adhika Balgobind Balakrishna Pillay discipline of microbiology School of Biochemistry Genetics Microbiology and Plant Pathology Faculty of Science and Agriculture University of KwaZulu-Natal (Westville Campus) Private Bag X54001 Durban 4000 Republic of South Africa

1,2-Dichloroethane （DCA）, a potential mutagen and carcinogen, is commonly introduced into the environment through its industrial and agricultural use. In this study, the impact of lead and mercury on DCA degradation in soil was investigated, owing to the complex co-contamination problem frequently encountered in most sites. 1,2-Dichloroethane was degraded readily in both contaminated loam and clay soils with the degradation rate constants ranging between 0.370-0.536 week-1 and 0.309-0.417 week-1, respectively. The presence of heavy metals have a negative impact on DCA degradation in both soil types, resulting in up to 24.11% reduction in DCA degradation within one week. Both biostimulation and treatment additives increased DCA degradation, with the best degradation observed upon addition of glucose and a combination of diphosphate salt and sodium chloride, leading to about 17.91% and 43.50% increase in DCA degradation, respectively. The results have promising potential for effective remediation of soils co-contaminated with chlorinated organics and heavy metals. However, the best bioremediation strategy will depend on the soil types, microbial population present in the soil matrices, nutrients availability and metal forms.

关键词： biodegradation biostimulation co-contamination heavy metals treatment additives

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Correlation between genotypes of Orientia tsutsugamushi and clinical characteristics of patients with scrub typhus in Guangzhou,China

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Asian Pacific Journal of Tropical Medicine 2024年第7期17卷 299-309,I0008,I0009页

作者： Jiali Long Ziyi Zeng Haiyan Chen Xia Tao Xinwei Wu Shouyi Chen Liqun Fang Xiuqing Zhang Jianxiong Xu Lin Zhang Yuehong Wei Guangzhou Center for Disease Control and Prevention GuangzhouChina Institute of Public Health Guangzhou Medical UniversityGuangzhouChina Outpatient department of Baogang the First Affiliated Hospital of Guangdong Pharmaceutical UniversityGuangzhouChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina Guangzhou Institute of Biomedicine and Health Chinese Academy of SciencesGuangzhouChina

Objective:To explore the correlation between genotypes of Orientia(O.)tsutsugamushi and clinical characteristics of scrub typhus ***:Clinical data of patients with scrub typhus admitted to different types of medical institutions in Guangzhou from September 2012 to December 2016 were collected using medical *** data,clinical manifestations,as well as hematological and biochemical indicators of patients infected with different genotypes were analyzed and ***:A total of 192 patients were included in this study,including 121 patients with Karp genotype of *** infection(63.0%),36 patients with Gilliam genotype(19.0%),23 patients with Kato genotype(12.0%),and 12 patients with TA763 genotype(6.0%)*** median value of albumin in patients with Karp genotype infection was significantly lower than that of Gilliam-infected patients(P=0.032).Patients with Karp genotype infection had a significantly longer hospital stay(9 days)than those with Gilliam genotype(7 days)(P=0.009)and Kato genotype infection(6 days)(P=0.005).Karp-infected patients also represented for the largest number of patients with complicated organ involvement(88/133,66.2%).Furthermore,Karp-infected patients had higher risk of developing multiple organ dysfunction syndrome(18.2%)and requiring intensive care unit treatment(15.9%).Besides,patients with Gilliam genotype(8 days)and TA763 genotype infection(7.5 days)had shorter fever duration than those with Karp genotype(9 days)and Kato genotype(9 days)infection,***:Genotypes of Orientia tsutsugamushi were associated with varying clinical manifestations,organ involvement,and treatment outcomes,suggesting that genotypes ranged in virulence.

关键词： Scrub typhus Orientia tsutsugamushi Genotype Clinical characteristics

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De-novo characterization of the soft-shelled turtle Pelodiscus sinensis transcriptome using Illumina RNA-Seq technology

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第1期14卷 58-67页

作者： Wei WANG Cai-yan LI Chu-tian GE Lei LEI You-ling GAO Guo-ying QIAN Zhejiang Provincial Top Key discipline of Modern microbiology and Application College of Biological and Environmental Sciences Zhejiang Wanli University Ningbo 315100 China Henan Provincial Bureau of Animal Husbandry Zhengzhou 450008 China

The soft-shelled turtle Pelodiscus sinensis is a high-profile turtle species because of its nutritional and medicinal value in Asian countries. However, little is known about the genes that are involved in formation of their nutritional quality traits, especially the molecular mechanisms responsible for unsaturated fatty acid and collagen biosynthesis. In the present study, the transcriptomes from six tissues from Pelodiscus sinensis were sequenced using an IIlumina paired-end sequencing platform. We obtained more than 47 million sequencing reads and 73954 unigenes with an average size of 754 bp by de-novo assembly. In total, 55.19% of the unigenes （40 814） had significant similarity with proteins in the National Center of Biotechnology Information （NCBI） non-redundant protein database and Swiss-Prot database （E-value 〈10-5）. Of these annotated unigenes, 9156 and 11 947 unigenes were assigned to 52 gene ontology categories （GO） and 25 clusters of orthologous groups （COG）, respectively. In total, 26496 （35.83%） unigenes were assigned to 242 pathways using the Kyoto Encyclopedia of Genes and Genomes pathway database （KEGG）. In addition, we found a number of highly expressed genes involved in the regulation of P. sinensis unsatu- rated fatty acid biosynthesis and collagen formation, including desaturases, growth factors, transcription factors, and extracellular matrix components. Our data represent the most comprehensive sequence resource available for the Chinese soft-shelled turtle and could provide a basis for new research on this turtle as well as the molecular genetics and functional genomics of other terrapins. To our knowledge, we report for the first time, the large-scale RNA se- quencing （RNA-Seq） of terrapin animals and would enrich the knowledge of turtles for future research.

关键词： Pelodiscus sinensis Illumina RNA-Seq Transcriptome Gene expression

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Zika virus in the testes： should we be worried？

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Protein & Cell 2017年第3期8卷 162-164页

作者： Gary Wong Shihua Li Lei Liu Yingxia Liu Yuhai Bi Shenzhen Key Laboratory of Pathogen and Immunity State Key Discipline of Infectious Disease Shenzhen Third People's_ Hospital Shenzhen 518112 China CAS Key Laboratory of Pathogenic microbiology and Immunology Institute of Microbiology Center for Influenza Research and Early-warning (CASCIRE) Chinese Academy of Sciences Beijing 100101 China

Zika virus （ZIKV） is a mosquito-borne pathogen from the family Flaviviridae, and infection of humans with ZIKV through mosquito bites may result in a disease known as Zika fever. Most Zika fever cases are asymptomatic and thus go unreported, but if symptoms do appear, they are usually mild in nature （fever, joint pain, headache and macu- Iopapular rash）, and self-resolve within one week （Chen and Hamer 2016）. No deaths have ever been attributed to Zika fever; however, when a woman is infected with ZIKV during pregnancy, vertical transmission of the virus may result in microcephaly in the offspring （Rasmussen, Jamieson et al., 2016）. ZIKV infections in adults are associated with episodes of Guillain-Barr6 syndrome, an autoimmune disorder of the peripheral nervous system, resulting in rapid-onset muscle weakness （Cao-Lormeau, Blake et al. 2016）.

关键词： Nonhuman Primate Dengue Virus Male Reproductive System Cynomolgus Macaque Vaginal Fluid

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Type Ⅰ interferon receptor knockout mice as models for infection of highly pathogenic viruses with outbreak potential

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Zoological Research 2018年第1期39卷 3-14页

作者： Gary Wong Xiang-Guo Qiu Shenzhen Key Laboratory of Pathogen and Immunity State Key Discipline of Infectious DiseaseShenzhen Third People's HospitalShenzhen Guangzhou 518020China Key Laboratory of Pathogenic microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Special Pathogens Program National Microbiology LaboratoryPublic Health Agency of CanadaWinnipegManitoba R3E 3R2Canada Department of Medical microbiology and Infectious Diseases University of ManitobaWinnipegManitoba R3E 0J9Canada

Due to their inability to generate a complete immune response, mice knockout for type I interferon （IFN） receptors （Ifnar-/-） are more susceptible to viral infections, and are thus commonly used for pathogenesis studies. This mouse model has been used to study many diseases caused by highly pathogenic viruses from many families, including the Flaviviridae, Filoviridae, Arenaviridae, Bunyaviridae, Henipaviridae, and Togaviridae. In this review, we summarize the findings from these animal studies, and discuss the pros and cons of using this model versus other known methods for studying pathogenesis in animals.

关键词： Ifnar Mice Animal model Flavivirus Filovirus Arenavirus Bunyavirus Henipavirus Togavirus

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Trends in Incidence of HIV and Syphilis Among Men Who Have Sex with Men—Tianjin Municipality,China,2013-2022

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China CDC weekly 2024年第27期6卷 651-657页

作者： Yan Guo Fangfang Chen Hui Gong Ziming Wu Xinying Zhang Tielin Ning Zhongquan Liu Jianyun Bai Long Li Jie Yang Fangning Zhao Jinyu Hou Maohe Yu Houlin Tang STD&AIDS Control and Prevention Section Tianjin Center for Disease Control and PreventionTianjinChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijingChina STD&AIDS Control and Prevention department Tianjin Hongqiao District Center for Disease Control and PreventionTianjinChina Tianjin Shenlan Public Health Counseling Service Center TianjinChina Tianjin Key Laboratory of Pathogenic microbiology of Infectious Disease Tianjin Centers for Disease Control and PreventionTianjinChina

Summary What is already known about this topic?Given the common modes of transmission,outbreaks of both human immunodeficiency virus(HIV)and syphilis are primarily observed in men who have sex with men(MSM).However,minimal research has been conducted to concurrently evaluate the rates and trends of HIV and syphilis incidence within this community in *** is added by this report?

关键词： Incidence primarily immunodeficiency

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Disseminated strongyloidiasis in an immunocompromised host:A case report

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Asian Pacific Journal of Tropical Biomedicine 2017年第6期7卷 587-590页

作者： Nurul Suhaiza Hassanudin Zubaidah Abdul Wahab Khalid Ibrahim Fadzilah Mohd Nor microbiology Unit Department of PathologyHospital Sg.Buloh Director Office Hospital Sg.Buloh microbiology discipline Faculty of MedicineUniversiti Teknologi MARASg.Buloh CampusJalan Hospital Integrative Pharmacogenomics Institute (iPROMISE) Level 7FF3Universiti Teknologi MARAPuncak Alam Campus

Infections caused by Strongyloides stercoralis(***) in human are generally asymptomatic,however in immunocompromised individual,hyperinfection may develop with dissemination of larvae to extra-intestinal *** diagnosis could be easily missed due to asymptomatic presentation and insufficient exposure towards the infection itself,which may lead to low index of suspicion as a *** this report,a case of a Malaysian male with underlying diabetes mellitus,hypertension,cerebrovascular accident,bullous pemphigus and syndrome of inappropriate antidiuretic hormone secretion who initially complained of generalized body weakness and poor appetite without any history suggestive of sepsis is ***,he developed septicemic shock later,and *** larvae was incidentally found in the tracheal aspirate that was sent to look for acid fast *** of aggressive resuscitation,the patient succumbed due to pulmonary hemorrhage and acute respiratory distress *** was revealed that the current case has alarmed us via incidental finding of *** larvae in the tracheal aspirate,indicating that the importance of the disease should be emphasized in certain parts of the world and population respectively.

关键词： Disseminated strongyloidiasis Immunocompromised host Hyperinfection Strongyloides stercoralis

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Animal models for filovirus infections

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Zoological Research 2018年第1期39卷 15-24页

作者： Vinayakumar Siragam Gary Wong Xiang-Guo Qiu Special Pathogens Program Public Health Agency of CanadaWinnipegManitoba R3E 3R2Canada Department of Medical microbiology University of ManitobaWinnipegManitoba R3E 0J9Canada Shenzhen Key Laboratory of Pathogen and Immunity State Key Discipline of Infectious DiseaseShenzhen Third People's HospitalShenzhen Guangzhou 51 8020China Key Laboratory of Pathogenic microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

The family Filoviridae, which includes the genera Marburgvirus and Ebolavirus, contains some of the most pathogenic viruses in humans and non-human primates （NHPs）, causing severe hemorrhagic fevers with high fatality rates. Small animal models against filoviruses using mice, guinea pigs, hamsters, and ferrets have been developed with the goal of screening candidate vaccines and antivirals, before testing in the gold standard NHP models. In this review, we summarize the different animal models used to understand filovirus pathogenesis, and discuss the advantages and disadvantages of each model with respect to filovirus disease research.

关键词： Filovirus Ebola virus Marburg virus Marburg virus disease Ebola virus disease Mouse-adapted ebolavirus Guinea-pig-adapted ebolavirus Sudan virus Plaque-forming units

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Molecular detection of virulence genes in Campylobacter species isolated from livestock production systems in South Africa

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Journal of Integrative Agriculture 2020年第6期19卷 1656-1670页

作者： Bongekile NGOBESE Oliver Tendayi ZISHIRI Mohamed Ezzat EL ZOWALATY discipline of Genetics School of Life SciencesUniversity of KwaZulu-NatalDurban 4000South Africa Zoonosis Science Center Department of Medical Biochemistry and MicrobiologyUppsala UniversityUppsala SE-75185Sweden Infectious Diseases and Anti-Infective Therapy Research Group College of Pharmacy and Sharjah Medical Research InstituteUniversity of SharjahSharjah 27272UAE

Campylobacter species are a major cause of foodborne bacterial infections in both developed and developing countries *** jejuni is responsible for the majority of infectio *** study was con ducted to identify virule nceassociated genes in Campylobacter species isolated from livestock production systems in South Africa.A total of 250 fecal samples consisting of cattle(n=50),chickens(n=50),goats(n=50),sheep(n=50)and pigs(n=50)were randomly collected from livestock in Eastern Cape and KwaZulu-Natal provinces of South Africa between April and October *** samples were an a lyzed for the presence of virule nee genes in Campylobacter species using molecular PCR-based *** was found that 77 and 23%of Campylobacter jejuni and Campylobacter coli respectively were isolated from all the livestock *** were positive significant(P<0.05)correlations amongst all the virulenee genes that were *** and Fisher's exact tests were implemented to test for the effect of livestock species on the presenee or absenee of virule nee gen *** study dem on strated that most of livestock species can pote ntially cause zoonotic infecti on s and food pois oning due to the high prevale nee of *** high prevale nee of virule nee genes highlights the sign ifica nee of Campylobacter\r\livestock production systems in South *** requires the implementation of one-health approaches to reduce the impact of foodborne and zoonotic diseases for the welfare of human and animal health.

关键词： Campylobacter zoonotic cytotoxicity pathogenicity virulenee campylobacteriosis foodbome livestock PCR one-health

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