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Altered mitochondria and Bcl-2 expression in the hippocampal CA3 region in a rat model of acute epilepsy

Neural Regeneration Research 2009年第4期4卷 276-280页

作者： Jiyan Cheng Lina Wu Qiaozhi Wang Yanfeng Gan Guangyi Liu Hong Yu Electron Microscopy Laboratory Luzhou Medical College Luzhou 646000 Sichuan Province China Department of Histology and Embryology Luzhou Medical College Luzhou 646000 Sichuan Province China

BACKGROUND： Previous studies have shown that the mitochondrial structure and function are damaged in animal models of epilepsy. In addition, the Bcl-2 protein is capable of regulating mitochondrial stability. OBJECTIVE： To observe and validate changes in mitochondrial structure and Bcl-2 expression, and to analyze these characteristics in the hippocampal CA3 region of rat models of epilepsy. DESIGN, TIME AND SETTING： This randomized, controlled, animal experiment was performed at the Laboratory of Electron Microscopy and Department of Histology and Embryology, luzhou Medical College between 2007 and 2008. MATERIALS： Coriamyrtin was provided by the Pharmacy Factory of West China University of Medical Sciences. The primary and secondary antibodies were provided by Zhongshan Goldenbridge Biotechnology, Beijing. METHODS： A total of 44 adult, male, Sprague Dawley rats were randomly divided into control （n = 11） and epilepsy （n = 33） groups. Rats in the epilepsy group were induced by coriamyrtin （50 μ g/kg）, which was injected into the lateral ventricles. The rats were then observed at 3, 6, and 24 hours after epilepsy induction, with 11 rats at each time point. Epilepsy was not induced in rats from the control group. MAIN OUTCOME MEASURES： Pathological changes in the hippocampal CA3 region were observed by light microscopy; Bcl-2 expression was analyzed by immunohistochemistry; and mitochondrial changes in the hippocampus were observed under transmission electron microscopy. RESULTS：（1） The control group displayed very little Bcl-2 protein expression in the hippocampal CA3 region. However, after 3 hours of epilepsy, expression was visible. By 6 hours, expression peaked and then subsequently decreased after 24 hours, but remained higher than the control group （P 〈 0.05）. （2） Mitochondria were damaged to varying degrees in the epilepsy groups. For example, mitochondria edema, cristae space increase, and disappearance of mitochondria were apparent. Moreover, mitochondrial

关键词： coriamyrtin epilepsy hippocampus immunohistochemistry mitochondrion rats

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Synaptic and blood-brain barrier structural changes in a rat epilepsy model induced by coriaria lacton Replication experiment with animals

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Neural Regeneration Research 2008年第1期3卷 25-28页

作者： Jiyan Cheng Jichun Huang Yi Han Guangyi Liu Ling Yin Furong Zheng Department of Histology and Embryology Luzhou Medical College Luzhou 646000 Sichuan Province China Electron Microscopy Laboratory Luzhou Medical College Luzhou 646000 Sichuan Province China

BACKGROUND： Structural and functional synaptic changes, as well as blood-brain barrier （BBB） changes, affect the micro-environment of nervous tissue and excitation, both of which play an important role in epilepsy. OBJECTIVE： To observe synaptic and BBB ultrastructural changes in the motor cortex of a rat epilepsy model induced by coriaria lacton, and to investigate the synaptic and BBB effects on the mechanism of epilepsy. DESIGN： A randomized controlled animal experiment. SETTING： Department of Histology and Embryology, luzhou Medical College; and Electron Microscopy Laboratory, luzhou Medical College. MATERIALS： Twenty healthy male Sprague Dawley rats, aged 8 weeks, were chosen for this study. The rats weighed （280 ± 50） g and were supplied by the Experimental Animal Center of luzhou Medical College. Experimentation was performed in accordance with the ethical guidelines for the use and care of animals. The animals were randomly divided into a control group and an epilepsy group, with 10 rats in each group. METHODS： This study was performed at the Department of Histology and Embryology, and Electron Microscopy Laboratory, luzhou Medical College between February and December 2006. According to the protocol, the epilepsy group was injected with 10 μ L/100 g coriaria lacton into the lateral ventricles to establish an epileptic model. The control group rats were not administered anything. Eight days after the model was established, all rats were anesthetized with ether. The motor cortex was removed and sectioned into ultrathin sections. Synaptic and BBB ultrastructural changes were observed by electron microscopy. MAIN OUTCOME MEASURES：（1）Structural changes of three different parts of the synapses, synaptic cleft width, postsynaptic density thickness, proportion of perforation synapses, curvature of synaptic interface, and length of active zones. （2）Capillary and BBB changes （endothelium, basement membrane, pericyte, and the astrocyte endfeet）. RESULTS：（1）Curvature

关键词： coriaria lacton epilepsy synapse blood-brain barrier rats

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Electromagnetic scattering and imaging simulation of extremely large-scale sea-ship scene based on GPU parallel technology

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Journal of Electronic Science and Technology 2024年第2期22卷 16-23页

作者： Cheng-Wei Zhang Zhi-Qin Zhao Wei Yang Li-Lai Zhou Hai-Yu Zhu Southwest China Institute of Electronic Technology Chengdu610036China School of Electronic Science and Engineering University of Electronic Science and Technology of ChinaChengdu611731China Tencent company Shenzhen518054China

Aiming to solve the bottleneck problem of electromagnetic scattering simulation in the scenes of extremely large-scale seas and ships,a high-frequency method by using graphics processing unit(GPU)parallel acceleration technique is *** the implementation of different electromagnetic methods of physical optics(PO),shooting and bouncing ray(SBR),and physical theory of diffraction(PTD),a parallel computing scheme based on the CPU-GPU parallel computing scheme is realized to balance computing ***,a multi-GPU framework is further proposed to solve the computational difficulty caused by the massive number of ray tubes in the ray tracing *** using the established simulation platform,signals of ships at different seas are simulated and their images are achieved as *** is shown that the higher sea states degrade the averaged peak signal-to-noise ratio(PSNR)of radar image.

关键词： Multi graphics processing unit Radar imaging Sea-ship Shooting and bouncing rays

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Primary or metastatic lung cancer?Sebaceous carcinoma of the thigh:A case report

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World Journal of Clinical Cases 2022年第14期10卷 4586-4593页

作者： Xiao-Lin Wei Qing Liu Qiang-Lin Zeng Hui Zhou Department of Respirology Taikang Sichuan Southwest Hospital Company LimitedChengdu 610095Sichuan ProvinceChina Department of Abdominal Oncology Cancer CenterWest China HospitalSichuan UniversityChengdu 610000Sichuan ProvinceChina Department of Respiratory and Critical Care Medicine The Affiliated Hospital of Chengdu University School of Clinical MedicineChengdu UniversityChengdu 610081Sichuan ProvinceChina

BACKGROUND Sebaceous carcinoma(SC),a malignancy primarily characterized by aggressive growth,affects cutaneous tissues of the periocular *** SC is extremely rare,especially in the extremities,as evidenced by only a handful of reported *** SUMMARY A 65-year-old man presented with a rapidly enlarging swelling on the left inner thigh,which was initially misdiagnosed as a subcutaneous *** lesion had appeared two months prior to *** examination revealed a cauliflower-like swollen content,with an ulcerated and infected mass located on his left *** the same time,we observed solitary nodular lesions in his lungs and brain,with biopsy pathology of the lung lesions found to be consistent with the mass in the *** patient received chemotherapy comprising cis-platinum with fluorouracil,followed by targeted therapy with anlotinib hydrochloride and chemotherapy with vinorelbine,implantation of iodine-125 seeds in the thigh and pulmonary *** initial stage intervention achieved partial *** efficacy of maintenance treatment was evaluated as stable disease after the first 5 cycles;however,the patient developed a new brain lesion after the sixth cycle of treatment,which resulted in progressive disease and he received whole brain gamma knife *** We analyzed the clinical presentation,imaging features,pathology and treatment of a rare case of lung,brain and lymph node metastasis of SC located in the *** is evident that cis-platinum combined with fluorouracil,vinorelbine combined with anlotinib hydrochloride may be an effective therapeutic regimen in advanced ***,brain metastatic lesions should receive early radiotherapy.

关键词： Extraocular sebaceous carcinoma Chemotherapy Targeted therapy Radiotherapy Case report

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Impairment of cognitive function and reduced hippocampal cholinergic activity in a rat model of chronic intermittent hypoxia

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Neural Regeneration Research 2011年第17期6卷 1301-1305页

作者： Chunling Zhao Yan Chen Chunlai Zhang Linya Lā Qian Xu Department of Physiology Luzhou Medical College Luzhou 646000 Sichuan Province China Department of Pediatric Surgery Affifiated Hospital of Luzhou Medical College Luzhou 646000 Sichuan Province China

The present study established a rat model of chronic intermittent hypoxia （CIH） to simulate obstructive sleep apnea syndrome. CIH rats were evaluated for cognitive function using the Morris water maze, and neuronal pathology in the hippocampus was observed using hematoxylin-eosin staining. In addition, hippocampal choline acetyl transferase （CHAT） and nicotinic acetylcholine receptor （nAChR） expression was determined by immunohistochemistry. Our results revealed necrotic hippocampal neurons, decreased ChAT and nAChR expression, as well as cognitive impairment in CIH rats. These results suggest that hippocampal neuronal necrosis and decreased cholinerqic activity may be involved in CIH-induced cognitive impairment in rats.

关键词： hypoxia brain memory dysfunction choline acetyl transferase nicotinic acetylcholinereceptor chronic intermittent hypoxia rat neural regeneration

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Effects of movement training on synaptic interface structure in the sensorimotor cortex and hippocampal CA3 area of the ischemic hemisphere in cerebral infarction rats

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Neural Regeneration Research 2008年第11期3卷 1203-1207页

作者： Min Yang Jiyan Cheng Department of Rehabifitation Medicine Affiliated Hospital Luzhou Medical College Luzhou 646000 Sichuan Province China Electron Microscopy Laboratory Luzhou Medical College Luzhou 646000 Sichuan Province China

BACKGROUND： Movement is an effective way to provide sensory, movement and reflectivity afferent stimulation to the central nervous system. Movement plays an important role in functional recombination and compensation in the brain. OBJECTIVE： To observe movement training effects on texture parameters of synaptic interfaces in the sensorimotor cortex and hippocampal CA3 area of the ischemic hemisphere and on motor function in cerebral infarction rats. DESIGN, TIME AND SETTING： This neural morphology and pathology randomized controlled animal experiment was performed at the Center Laboratory, Affiliated Hospital of luzhou Medical College, China from November 2004 to April 2005. MATERIALS： A total of 32 healthy male Wistar rats aged 8 weeks were equally and randomly assigned into model and movement training groups. METHODS： Rat models of right middle cerebral artery occlusion were established using the suture occlusion method in both groups. Rats in the movement training group underwent balance training, screen training, and rotating rod training starting on day 5 after surgery, for 40 minutes every day, 6 days per week, for 4 weeks. MAIN OUTCOME MEASURES： Texture parameters of synaptic interfaces were determined using a transmission electron microscope and image analyzer during week 5 following model induction. The following parameters were measured： synaptic cleft width; postsynaptic density thickness; synaptic interface curvature; and active zone length. Motor function was assessed using balance training, screen training, and rotating rod training. The lower score indicated a better motor function. RESULTS： The postsynaptic density thickness, synaptic interface curvature, and active zone length were significantly increased in the sensorimotor cortex and hippocampal CA3 area of the ischemic hemisphere of rats from the movement training group compared with the model group （P 〈 0.05 or 0.01）. Curved synapses and perforated synapses were seen in the sensorimotor cortex a

关键词： cerebral infarction movement training synapse rats

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Protein Disulfide Isomerase A2 Is Correlated with Immune Infiltrates and Is a Novel Prognostic Biomarker in Glioma Patients

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Current Medical Science 2023年第6期43卷 1107-1115页

作者： Zhi-gang MA Ying-xue LIU Ning ZOU Zhe HUANG Ming WANG Tao LI Jie ZHOU Li-gang CHEN Department of Neurosurgery The Affiliated Hospital of Southwest Medical UniversityLuzhou646000China sichuan Clinical Medical Research Center for Neurosurgery Luzhou646000China School of Clinical Medicine Southwest Medical UniversityLuzhou646000China Department of Laboratory People’s Hospital of Deyang CityDeyang618000China Laboratory of Mitochondria and Metabolism Department of AnesthesiologyNational Clinical Research Center for GeriatricsWest China Hospital of Sichuan UniversityChengdu610041China Neurological Diseases and Brain Function Laboratory The Affiliated Hospital of Southwest Medical UniversityLuzhou646000China Academician(Expert)Workstation of sichuan province The Affiliated Hospital of Southwest Medical UniversityLuzhou646000China

Objective Protein disulfide isomerase A2(PDIA2),a member of the protein disulfide isomerase family,plays a key role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds,together with enzymes such as thiol isomerase,oxidase,and *** study investigated the clinical significance and potential functions of PDIA2 in *** The expression of PDIA2 in gliomas was explored using The Cancer Genome Atlas and Gene Expression Omnibus *** analyzed the clinical characteristics of glioma patients and the prognostic and diagnostic value of PDIA2 ***-Meier and Cox regression analyses were used to examine the effect of PDIA2 expression on overall survival,progression-free interval,and disease-specific ***,we performed Gene Set Enrichment Analysis and immune infiltration analysis to investigate the functions of ***2 mRNA and protein expression was evaluated in cell lines and glioma *** PDIA2 was expressed at low levels in glioma ***-Meier survival analysis showed that glioma patients with low PDIA2 levels had a worse prognosis than those with high PDIA2 *** operating characteristic curve analysis indicated the diagnostic and prognostic ability of PDIA2(area under the curve=0.918).Pathways associated with PD1,PI3K/AKT,cancer immunotherapy via PD1 blockade,Fceri-mediated NF-kB activation,FOXM1,and DNA repair were enriched in glioma patients with low levels of ***2 expression levels were negatively correlated with immune cell infiltrate *** PDIA2 levels are significantly downregulated in ***2 expression may be a potential biomarker for the diagnosis and prognosis of glioma patients.

关键词： glioma protein disulfide isomerase A2 bioinformatics diagnosis prognosis

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Extracranial multiorgan metastasis from primary glioblastoma: A case report

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World Journal of Clinical Cases 2021年第33期9卷 10300-10307页

作者： Xing-Zhao Luan Hao-Run Wang Wei Xiang Shen-Jie Li Haiping He Li-Gang Chen Jian-Mei Wang Jie Zhou Department of Neurosurgery The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina sichuan Clinical Research Center for Neurosurgery Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Academician(Expert)Workstation of sichuan province Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Pathology The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina

BACKGROUND Glioblastoma has a high degree of malignancy and poor *** is common to have in situ recurrence and intracranial metastasis,while extracranial metastasis is rare,and extracranial multiorgan metastasis is extremely *** report a case of glioblastoma with extracranial multiorgan metastasis,which will strengthen clinicians’attention to the extracranial metastasis of glioblastoma and its *** SUMMARY A male patient visited our hospital for treatment of dizziness and *** resonance imaging of the brain revealed a space-occupying lesion in the right temporoparietal occipital *** computed tomography and abdominal ultrasound were normal,and no space-occupying lesions were observed in other organs of the *** patient underwent surgery and diagnosed with *** concurrent radiotherapy and chemotherapy were *** the follow-up,the tumor was found to have metastasized to the scalp and neck,and a second tumor resection was *** follow-up revealed extracranial metastases to multiple extracranial organs including skull,scalp,ribs,spine,liver and *** family members refused further treatment,and requested only symptomatic treatment such as pain relief,and the patient died of systemic multiple organ *** time from diagnosis to death was 13 mo and from extracranial metastasis to death was 6 *** Glioblastoma extracranial metastasis is extremely rare,clinicians should always pay attention to its *** mechanism of glioblastoma extracranial metastasis is still unclear,and genetic and molecular studies are required.

关键词： Glioblastoma Extracranial metastasis Multiple organ metastasis Primary glioblastoma Case report

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Culture and identification of neonatal rat brain-derived neural stem cells

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World Journal of Stem Cells 2023年第6期15卷 607-615页

作者： Qing-Zhong Zhou Xiao-Lan Feng Xu-Feng Jia Nurul Huda Binti Mohd Nor Mohd Hezery Bin Harun Da-Xiong Feng Wan Aliaa Wan Sulaiman Department of Orthopedics The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Neurology Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia Department of Radiology The Affiliated Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Orthopedics The Peoples’Hospital of Jianyang CityJianyang 641400Sichuan ProvinceChina Department of Human Anatomi Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang Selangor43400Malaysia Department of Orthopedics Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400SelangorMalaysia

BACKGROUND Timing of passaging,passage number,passaging approaches and methods for cell identification are critical factors influencing the quality of neural stem cells(NSCs)*** to effectively culture and identify NSCs is a continuous interest in NSCs study while these factors are comprehensively *** To establish a simplified and efficient method for culture and identification of neonatal rat brain-derived *** First,curved tip operating scissors were used to dissect brain tissues from new born rats(2 to 3 d)and the brain tissues were cut into approximately 1 mm^(3)*** the single cell suspension through a nylon mesh(200-mesh)and culture the sections in *** was conducted with TrypLTM Express combined with mechanical tapping and pipetting ***,identify the 5th generation of passaged NSCs as well as the revived NSCs from *** incorporation method was used to detect self-renew and proliferation capabilities of *** NSCs specific antibodies(anti-nestin,NF200,NSE and GFAP antibodies)were used to identify NSCs specific surface markers and muti-differentiation capabilities by immunofluorescence *** Brain derived cells from newborn rats(2 to 3 d)proliferate and aggregate into spherical-shaped clusters with sustained continuous and stable *** BrdU was incorporated into the 5th generation of passaged cells,positive BrdU cells and nestin cells were observed by immunofluorescence *** induction of dissociation using 5%fetal bovine serum,positive NF200,NSE and GFAP cells were observed by immunofluorescence *** This is a simplified and efficient method for neonatal rat brain-derived neural stem cell culture and identification.

关键词： Neonatal rats Brain-derived neural stem cells Culture Identification

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Screening of the ubiquitin-proteasome system activators for anti-Alzheimer’s disease by the high-content fluorescence imaging system

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Chinese Journal of Natural Medicines 2022年第1期20卷 33-42页

作者： WANG Yi-Ling YOU Jing CAO Jing-Jie LI Wei JING Liu-Yang MEI Qi-Bing WU An-Guo sichuan Key Medical Laboratory of New Drug Discovery and Drugability Evaluation Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia MedicaSchool of PharmacyEducation Ministry Key Laboratory of Medical ElectrophysiologySouthwest Medical UniversityLuzhou 646000China luzhou New Drug Evaluation and Research Center Luzhou 646000China

Ubiquitin-proteasome system(UPS)plays an important role in neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),and Huntington’s disease(HD).The discovery of UPS activators for anti-neurodegenerative diseases is becoming increasingly *** this study,we aimed to identify potential UPS activators using the high-throughput screening method with the high-content fluorescence imaging system and validate the neuroprotective effect in the cell models of *** first,stable YFP-CL1 HT22 cells were successfully constructed by transfecting the YFP-CL1 plasmid into HT22 cells,together with G418 *** degradation activity of the test compounds via UPS was monitored by detecting the YFP fluorescence intensity reflected by the ubiquitin-proteasome degradation signal *** employing the high-content fluorescence imaging system,together with stable YFP-CL1 HT22 cells,the UPS activators were successfully screened from our established TCM *** representative images were captured and analyzed,and quantification of the YFP fluorescence intensity was performed by flow ***,the neuroprotective effect of the UPS activators was investigated in pEGFP-N1-APP(APP),pRK5-EGFP-Tau P301L(Tau P301L),or pRK5-EGFP-Tau(Tau)transiently transfected HT22 cells using fluorescence imaging,flow cytometry,and Western *** conclusion,our study established a high-content fluorescence imaging system coupled with stable YFP-CL1 HT22 cells for the highthroughput screening of the UPS *** compounds,namely salvianolic acid A(SAA),salvianolic acid B(SAB),and ellagic acid(EA),were identified to significantly decrease YFP fluorescence intensity,which suggested that these three compounds are UPS *** identified UPS activators were demonstrated to clear AD-related proteins,including APP,Tau,and Tau ***,these findings provide a novel insight into the discovery and development of anti-AD drugs.

关键词： Ubiquitin-proteasome system High-content fluorescence imaging system Traditional Chinese medicine Alzheimer’s disease YFP-CL1 HT22 cells

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