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Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing

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Genes & diseases 2023年第6期10卷 2597-2613页

作者： Xin Qiu Hao-Yu Wang Zhen-Yu Yang Li-Ming Sun Shu-Nan Liu Chui-Qin Fan Feng Zhu Department of Spinal Surgery The University of Hong Kong-Shenzhen HospitalShenzhenGuangdong 518000China Department of Orthopedics and Traumatology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China College of Life Sciences University of Chinese Academy of SciencesBeijing 100000China Lars Bolund Institute of Regenerative Medicine Qingdao-Europe Advanced Institute for Life SciencesBGI-QingdaoQingdaoShandong 266000China China Medical University ShenyangLiaoning 110000China Department of Plastic and Reconstructive Surgery Xijing HospitalForth Military Medical UniversityXi'anShaanxi 710000China

To uncover the role of satellite cells(SCs)in paravertebral muscle development and aging,we constructed a single-nucleus transcriptomic atlas of mouse paravertebral muscle across seven timepoints spanning the embryo(day 16.5)to old(month 24)stages.Eight cell types,including SCs,fast muscle cells,and slow muscle cells,were identified.An energy metabolism-related gene set,TCA CYCLE IN SENESCENCE,was enriched in SCs.Forty-two skeletal muscle disease-related genes were highly expressed in SCs and exhibited similar expression patterns.Among them,Pdha1 was the core gene in the TCA CYCLE IN SENESCENCE;Pgam2,Sod1,and Suclg1 are transcription factors closely associated with skeletal muscle energy metabolism.Transcription factor enrichment analysis of the 42 genes revealed that Myod1 and Mef2a were also highly expressed in SCs,which regulated Pdha1 expression and were associated with skeletal muscle development.These findings hint that energy metabolism may be pivotal in SCs development and aging.Three ligand-receptor pairs of extracellular matrix(ECM)-receptor interactions,Lamc1-Dag1,Lama2-Dag1,and Hspg2-Dag1,may play a vital role in SCs interactions with slow/fast muscle cells and SCs self-renewal.Finally,we built the first database of a skeletal muscle single-cell transcriptome,the Musculoskeletal Cell Atlas(http://www.***.tech),which lists 630,040 skeletal muscle cells and provides interactive visualization,a useful resource for revealing skeletal muscle cellular heterogeneity during development and aging.Our study could provide new targets and ideas for developing drugs to inhibit skeletal muscle aging and treat skeletal muscle diseases.

关键词： Niche Paraspinal muscle Satellite cells Senescence Single-nucleus RNA sequencing

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Research advances in pathogenic mechanisms underlying air pollution-induced ocular surface diseases

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Advances in Ophthalmology Practice and Research 2021年第1期1卷 1-4页

作者： Fan Song Shengjie Hao Yuzhou Gu Ke Yao Qiuli Fu Eye Center of the 2nd Affiliated Hospital School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Zhejiang provincial key Lab of Ophthalmology HangzhouZhejiang ProvinceChina

Background:The harmful effect of aerial fine particulate matter(PM_(2.5))has been a serious public health issue and has attracted worldwide attention,especially in developing countries.Main Text:Numerous previous clinical and experimental studies have demonstrated that PM_(2.5) has a clear pathogenic effect on diseases related to the respiratory and cardiovascular systems.Recent researches have pointed out that PM_(2.5) plays a pivotal role in the occurrence and progression of ocular surface diseases.The current studies have shown that PM_(2.5) may promote the appearance of conjunctivitis,keratitis,blepharitis,dry eye,meibomian gland dysfunction(MGD)and other ocular surface diseases through regulating a series of mechanisms such as inflammation,immune reaction,oxidative stress,autophagy,cell migration,and epigenetics.Conclusions:This review aims to summarize the current research progress on the pathogenic mechanism of PM_(2.5)-related ocular surface diseases.

关键词： PM_(2.5) Mechanism Ocular surface diseases Air pollutuion

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Effects of Extrusion Speed on the Microstructure and Mechanical Properties of Mg-9Gd-3Y-1.5Zn-0.8Zr alloy

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Journal of Materials Science & Technology 2016年第4期32卷 313-319页

作者： Xuan Liu Zhiqiang Zhang Wenyi Hu Qichi Le Lei Bao Jianzhong Cui key Lab of Electromagnetic Processing of Materials Ministry of Education Northeastern University Shenyang 110819 China

The effects of the extrusion speed on the microstructures, mechanical properties and aging hardening behaviors of Mg-9Gd-3Y-1.5Zn-0.8Zr alloy have been investigated. The microstructure evolution during hot extrusion has also been discussed. The microstructures of the extruded alloys mainly consist of the equiaxed dynamically recrystallized grains and fiber-like long period stacking ordered （LPSO） phase. The increasing extrusion speed results in grain coarsening and the mechanical properties deterioration. Mean- while, it could also retard the aging hardening response. The extrudability of the investigated alloy is limited. Cracks nucleate and grow up rapidly when the extrusion speed is over 0.3 m/min. It should be closely related to the large amount of LPSO phase and the unique microstructure evolution. The rapidly precipitating fine lamella during extrusion could pin the dislocations and grain boundaries effectively. It could strengthen the alloy but also limit the extrudability of the investigated alloy.

关键词： Mg--Gd--Y--Zn--Zr alloyHot extrusionExtrusion speedMicrostructuresAging hardeningMechanical properties

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血清免疫球蛋白GN-糖基的高通量分析——一种消化道癌症的非侵入性生物标志物

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Engineering 2023年第7期26卷 44-53,I0002,I0003页

作者：刘鹏程王小兵顿爱社李昱潼李厚强王璐张怡春李灿灿张金霞张晓雨马立兴侯海峰 School of Public Health Shandong First Medical University&Shandong Academy of Medical SciencesJinan 250117China State key laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China School of Stomatology Shandong First Medical University&Shandong Academy of Medical SciencesJinan 250117China Beijing key laboratory of Clinical Epidemiology School of Public HealthCapital Medical UniversityBeijing 100069China Beijing Sanbo Brain Hospital Capital Medical UniversityBeijing 100093China Department of Gastroenterology The Second Affiliated Hospital of Shandong First Medical UniversityTaian 271000China Department of Epidemiology School of Public HealthShandong First Medical University&Shandong Academy of Medical SciencesJinan 250117China

免疫球蛋白G(Immunoglobulin G,IgG)的N-糖基化在炎症性疾病的发展中起着重要作用。本研究旨在评价IgG N-糖基在消化道癌症亚型中的诊断效能。从中国医学科学院肿瘤医院招募749名消化道癌症患者,包括食管癌(esophageal cancer,EC)、胃癌... 详细信息

免疫球蛋白G(Immunoglobulin G,IgG)的N-糖基化在炎症性疾病的发展中起着重要作用。本研究旨在评价IgG N-糖基在消化道癌症亚型中的诊断效能。从中国医学科学院肿瘤医院招募749名消化道癌症患者,包括食管癌(esophageal cancer,EC)、胃癌(gastric cancer,GC)、结直肠癌(colorectal cancer,CRC)和胰腺癌(pancreatic cancer,PC)患者。采用亲水交互高效液相色谱-超高效液相色谱(hydrophilic interaction liquid chromatography using ultra-performance liquid chromatography,HILIC-UPLC)分析血浆中IgG的N-糖基构成。采用Bio-Plex悬液芯片系统检测方法(Bio-Rad)进行炎症因子检测。采用典型相关分析(canonical correlation analysis,CCA)探索糖基和炎症因子之间的相关性。采用LASSO回归和logistic回归模型,基于检测到的糖基谱建立可用于区分胃肠癌症患者和健康人群诊断模型。与健康对照组相比,EC、GC、CRC和PC患者的唾液酸化和半乳糖基化水平降低,而二等分乙酰葡萄糖胺基化水平在消化道癌症患者中升高。此外,只有胰腺癌患者具有低水平的岩藻糖基化。消化道癌症组的IL-1β、IL-31和sCD40L水平均高于对照组。IgG N-糖基的组成与炎症因子相关(r=0.556)。基于糖基的模型表现出良好的诊断效能,EC、GC、CRC和PC的受试者工作特征曲线下面积(AUC)分别为0.972、0.871、0.867和0.907。这些研究结果表明,IgG N-糖基在调节消化道肿瘤的发病机制中发挥了重要作用。血清IgG N-糖基可以作为潜在的非侵入性辅助消化道癌症临床诊断的方法。

关键词： Gastrointestinal cancer Glycosylation Immunoglobulin G Diagnostic biomarker

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The optimal vaccination strategy to control COVID-19:a modeling study in Wuhan City,China

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Infectious diseases of Poverty 2021年第6期10卷 48-73页

作者： Ze-yu Zhao Yan Niu Li Luo Qing-qing Hu Tian-long Yang Mei-jie Chu Qiu-ping Chen Zhao Lei Jia Rui Cheng‑long Song Sheng‑nan Lin Yao Wang Jing‑wen Xu Yuan‑zhao Zhu Xing‑chun Liu Meng Yang Jie‑feng Huang Wei‑kang Liu Bin Deng Chan Liu Zhuo‑yang Li Pei‑hua Li Yan-hua Su Ben-hua Zhao Wen-long Huang Roger Frutos Tian-mu Chen State key laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthXiamen University4221‑117 South Xiang’an RoadXiang’an DistrictXiamen 361102FujianPeople’s Republic of China Cirad UMR 17IntertrypUniversitéde MontpellierMontpellierFrance Chinese Center for Disease Control and Prevention BeijingPeople’s Republic of China Division of Public Health School of MedicineUniversity of UtahUtahUSA Medical Insurance Ofce Xiang’an Hospital of Xiamen UniversityXiamenFujianPeople’s Republic of China Department of Data Science College of Natural SciencesColorado State UniversityFort CollinsCOUSA Fujian provincial Center for Disease Control and Prevention 76 Jintai RoadGulou DistrictFuzhouFujianPeople’s Republic of China

Background: Reaching optimal vaccination rates is an essential public health strategy to control the coronavirus disease 2019 (COVID-19) pandemic. This study aimed to simulate the optimal vaccination strategy to control the disease by developing an age-specific model based on the current transmission patterns of COVID-19 in Wuhan City, China.Methods: We collected two indicators of COVID-19, including illness onset data and age of confirmed case in Wuhan City, from December 2, 2019, to March 16, 2020. The reported cases were divided into four age groups: group 1, ≤ 14 years old;group 2, 15 to 44 years old;group 3, 44 to 64 years old;and group 4, ≥ 65 years old. An age-specific susceptible-exposed-symptomatic-asymptomatic-recovered/removed model was developed to estimate the transmissibility and simulate the optimal vaccination strategy. The effective reproduction number (R_(eff)) was used to estimate the transmission interaction in different age groups.Results: A total of 47 722 new cases were reported in Wuhan City from December 2, 2019, to March 16, 2020. Before the travel ban of Wuhan City, the highest transmissibility was observed among age group 2 (R_(eff) = 4.28), followed by group 2 to 3 (R_(eff) = 2.61), and group 2 to 4 (R_(eff) = 1.69). China should vaccinate at least 85% of the total population to interrupt transmission. The priority for controlling transmission should be to vaccinate 5% to 8% of individuals in age group 2 per day (ultimately vaccinated 90% of age group 2), followed by 10% of age group 3 per day (ultimately vaccinated 90% age group 3). However, the optimal vaccination strategy for reducing the disease severity identified individuals ≥ 65 years old as a priority group, followed by those 45-64 years old.Conclusions: Approximately 85% of the total population (nearly 1.2 billion people) should be vaccinated to build an immune barrier in China to safely consider removing border restrictions. Based on these results, we concluded that 90% of adults aged 15-64 years should first be vaccinated t

关键词： SARS-CoV-2 Transmissibility Age-specific model Vaccination strategy Effectiveness

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Traditional Chinese medicine: a promising candidate for the treatment of Alzheimer’s disease

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Translational Neurodegeneration 2013年第1期2卷 30-36页

作者： Zhi-Kun Sun Hong-Qi Yang Sheng-Di Chen Department of Neurology Henan Provincial People’s HospitalZhengzhou 450003 Henan ProvincePeople’s Republic of China Department of Neurology and Institute of Neurology Ruijin HospitalShanghai JiaoTong University School of MedicineShanghai 200025People’s Republic of China

Alzheimer’s disease(AD)is an age-related neurodegenerative disorder,characterized clinically by insidious onset of memory and cognition impairment,emergence of psychiatric symptoms and behavioral disorder,and impairment of activities of daily living(ADL).Traditional Chinese medicine(TCM)is practiced in the Chinese health care system for more than 2,000 years.In recent years,scientists have isolated many novel compounds from herbs,some of which improve dementia with fewer side effects than conventional drugs and are regarded as potential anti-AD drugs.In this review,we summarize the latest research progress on TCM showing their possible role of treatment of AD and other demented diseases and possible pharmacological actions.

关键词： Alzheimer’s disease Chinese traditional medicine Therapeutic approach

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Transcriptome and differential expression genes analysis of resistant and susceptible eggplant roots challenged with Ralstonia solanacearum

Transcriptome and differential expression genes analysis of ...

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中国植物病理学会2015年学术年会

作者：佘小漫何自福 DU Zheng-guo TANG Ya-fei LAN Guo-bin Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangdong provincial key laboratory of High Technology for Plant Protection

Bacterial wilt of eggplant caused by Ralstonia solanacearum is one of the most devastating diseases in the worldwide.Cultivars S47 A and 0802 exhibited resistant and susceptible to bacterial wilt in greenhouse and fields,respectively.To know the resistance mechanism of eggplant against R.solanacearum,using Illumina Solexa sequencing technology,transcriptomes of S47 A and 0802 were sequenced and their gene expression profile changes were characterized at 3h,6h,12 h and 24 h after infection with R.solanacearum.A total of 26521 unigenes which were assigned with at least one GO term,can be categorized into 45 functional groups.A total of 15299 unigenes had a KOG classification.A total of 15452 unigenes could be annotated in KEGG database.The differential expression genes were analyzed between Sm08023 and Sm08020,Sm08026 and Sm08020,Sm080212 and Sm08020,Sm080224 and Sm08020,SmS47A3 and SmS47A0,SmS47A6 and SmS47A0,SmS47A12 and SmS47A0,SmS47A24 and SmS47A0,respectively.The results revealed that 765,1371,1384,1257 DEGs were up-regulated,and 787,2159,2824,3047 DEGs were down-regulated at 3h,6h,12 h and 24 h post inoculation in cultivar 0802,respectively,while 943,1084,1136,1194 DEGs were up-regulated,and 759,1549,2372,2777 DEGs were down-regulated at 3h,6h,12 h and 24 h post inoculation in cultivar S47 A.These results will help to gain more information about the resistance mechanism of eggplant against R.solanacearum.

关键词： eggplant transcriptome differential expression genes resistant cultivar susceptible cultivar

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A novel polyethyleneimine-decorated FeOOH nanoparticle for efficient siRNA delivery

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Chinese Chemical Letters 2021年第1期32卷 102-106页

作者： Shuai Guo Bei Liu Mengjie Zhang Chunhui Li Xiaoxia Wang Yuhua Weng Lele Li Yuanyu Huang School of Life Science Advanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyInstitute of Engineering MedicineBeijing Institute of TechnologyBeijing 100081China CAS key laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and Technology(NCNST)Beijing 100190China School of Pharmacy Hunan University of Chinese MedicineChangsha 410208China College of Materials Science and Optoelectronic Technology University of Chinese Academy of SciencesBeijing 100049China Hunan University of Science and Engineering Yongzhou 425199China Institute of Molecular Medicine Peking UniversityBeijing 100871China

Despite the promising prospect of small interfering RNA(siRNA) for the treatment of diverse diseases,it remains challenging to develop novel delive ry materials to desired tissues and cells.In this study,a novel iron oxyhydroxide(FeOOH) nanoparticle(NP) whose surface was modified with branched polyetherimide(PEI) was developed to deliver siRNA into the cancer cells.It was demonstrated that PEI-FeOOH(PFeOOH) efficiently complexed siRNA,mediated effective cellular uptake and endosomal escape,thereby triggering robust gene silencing in vitro.In addition,PFeOOH/siRNA formulation loading with anti-RRM2 siRNA effectively inhibited the growth of tumor tissues,and exhibited excellent safety profiles in vivo.Therefore,this study conceptually provided a FeOOH-based nucleic acid delivery vesicle which can potentially use to achieve diagnosis and therapy simultaneously.

关键词： FeOOH siRNA delivery RRM2 RNA interference Polyetherimide Cancer treatment

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Research trends, hot spots and prospects for necroptosis in the field of neuroscience

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Neural Regeneration Research 2021年第8期16卷 1628-1637页

作者： Wei-Tao Yan Shuang Lu Yan-Di Yang Wen-Ya Ning Yan Cai Xi-Min Hu Qi Zhang Kun Xiong Department of Anatomy and Neurobiology School of Basic Medical ScienceCentral South UniversityChangshaHunan ProvinceChina Third Xiangya Hospital of Central South University ChangshaHunan ProvinceChina Department of Histology and Embryology School of Basic Medical ScienceCentral South UniversityChangshaHunan ProvinceChina Hunan key laboratory of Ophthalmology ChangshaHunan ProvinceChina

There are two types of cell death-apoptosis and necrosis. Apoptosis is cell death regulated by cell signaling pathways, while necrosis has until recently been considered a passive mechanism of cell death caused by environmental pressures. However, recent studies show that necrosis can also be regulated by specific cell signaling pathways. This mode of death, termed necroptosis, has been found to be related to the occurrence and development of many diseases. We used bibliometrics to analyze the global output of literature on necroptosis in the field of neuroscience published in the period 2007–2019 to identify research hotspots and prospects. We included 145 necroptosisrelated publications and 2239 references published in the Web of Science during 2007–2019. Visualization analysis revealed that the number of publications related to necroptosis has increased year by year, reaching a peak in 2019. China is the country with the largest number of publications. key word and literature analyses demonstrated that mitochondrial function change, stroke, ischemia/reperfusion and neuroinflammation are likely the research hotspots and future directions of necroptosis research in the nervous system. The relationship between immune response-related factors, damage-associated molecular patterns, pathogen-associated molecular patterns and necroptosis may become a potential research hotspot in the future. Taken together, our findings suggest that although the inherent limitations of bibliometrics may affect the accuracy of the literature-based prediction of research hotspots, the results obtained from the included publications can provide a reference for the study of necroptosis in the field of neuroscience.

关键词： bibliometric analysis citations CiteSpace h-index necroptosis network analysis neuroscience output VOSviewer Web of Science

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An Association between Immunosenescence and CD4^+CD25^+ Regulatory T Cells: A Systematic Review

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Biomedical and Environmental Sciences 2010年第4期23卷 327-332页

作者： LING WANG YAN XIE LI-JING ZHU TING-TING CHANG YAN-QING MAO JIE LI Clinical Immunology laboratory The First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu China

Objective Age-related increment of the prevalence of CD4^＋CD25^＋ regulatory T （Treg） cells were described controversially, and whether such changes explain immune dysfunction in the elderly is still unclear. The aim of this systematic review is to evaluate the role of the Tregs in immunosenescence. Methods Medline and manual searches were performed to identify all published epidemiological and animal studies investigating the efficacy of the association between immunosenescence and Treg cells. Results It was founded that the frequency, phenotypic characteristics, and number/function of Tregs were altered significantly with aging. Medical conditions in individuals with advanced ageas well as apoptosis intensity of Treg cells had an impact on the accumulation of Tregs which in turn could deteriorate cytotoxic activity of CD8＋ T and NK cells and production of IL-2. The range of immune cells that could be suppressed by Treg cells was quite wide and covered CD4^＋CD25^＋ T cells, NK cells, dendritic cells and even monocytes. These changes were observed both in humans and experimental animals. Besides, it was believed that frequency of Tregs increased with age and was accompanied by intensified suppressive activity for Tregs in patients, for example, with Alzheimer disease （AD） and Parkinson disease （PD）. The impaired condition of CD4＋ T cells, so-called immunosenescence, rendered transplant recipients less responsive to an allogeneic kidney graft, an effect that was limited to transplant recipients who were aged over 60 years. Conclusions Treg cells are associated with immunosenescence. All these changes contribute to the aging-related decline of immune responses and lead to the higher risk of immune-mediated diseases, cancer or infections in aged individuals.

关键词： Aging Immunosenescence CD4^＋CD25^＋ T cell Treg Case-control studies Cohort studies Cross-sectional studies

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