Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing
作者机构：Department of Spinal SurgeryThe University of Hong Kong-Shenzhen HospitalShenzhenGuangdong 518000China Department of Orthopedics and TraumatologyLi Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China College of Life SciencesUniversity of Chinese Academy of SciencesBeijing 100000China Lars Bolund Institute of Regenerative MedicineQingdao-Europe Advanced Institute for Life SciencesBGI-QingdaoQingdaoShandong 266000China China Medical UniversityShenyangLiaoning 110000China Department of Plastic and Reconstructive SurgeryXijing HospitalForth Military Medical UniversityXi'anShaanxi 710000China
出 版 物：《Genes & Diseases》 (基因与疾病(英文))
年 卷 期：2023年第10卷第6期
页 面：2597-2613页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by the Shenzhen Fundamental Research Program,China(No.JCYJ20180306173518936) Sanming Project of Medicine in Shenzhen,China(No.SZSM201612055) HKU-SZH Fund for Shenzhen Key Medical Discipline(China)(No.SZXK2020084) China National GeneBank(CNGB)
主 题：Niche Paraspinal muscle Satellite cells Senescence Single-nucleus RNA sequencing
摘 要：To uncover the role of satellite cells(SCs)in paravertebral muscle development and aging,we constructed a single-nucleus transcriptomic atlas of mouse paravertebral muscle across seven timepoints spanning the embryo(day 16.5)to old(month 24)*** cell types,including SCs,fast muscle cells,and slow muscle cells,were *** energy metabolism-related gene set,TCA CYCLE IN SENESCENCE,was enriched in ***-two skeletal muscle disease-related genes were highly expressed in SCs and exhibited similar expression *** them,Pdha1 was the core gene in the TCA CYCLE IN SENESCENCE;Pgam2,Sod1,and Suclg1 are transcription factors closely associated with skeletal muscle energy *** factor enrichment analysis of the 42 genes revealed that Myod1 and Mef2a were also highly expressed in SCs,which regulated Pdha1 expression and were associated with skeletal muscle *** findings hint that energy metabolism may be pivotal in SCs development and *** ligand-receptor pairs of extracellular matrix(ECM)-receptor interactions,Lamc1-Dag1,Lama2-Dag1,and Hspg2-Dag1,may play a vital role in SCs interactions with slow/fast muscle cells and SCs ***,we built the first database of a skeletal muscle single-cell transcriptome,the Musculoskeletal Cell Atlas(http://***),which lists 630,040 skeletal muscle cells and provides interactive visualization,a useful resource for revealing skeletal muscle cellular heterogeneity during development and *** study could provide new targets and ideas for developing drugs to inhibit skeletal muscle aging and treat skeletal muscle diseases.
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