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MiRNA 24-3p-rich exosomes functionalized DEGMA-modified hyaluronic acid hydrogels for corneal epithelial healing

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Bioactive Materials 2023年第7期25卷 640-656页

作者： Xiaomin Sun Wenjing Song Lijing Teng Yongrui Huang Jia Liu Yuehai Peng Xiaoting Lu Jin Yuan Xuan Zhao Qi Zhao Yingni Xu Jingjie Shen Xiaoyun Peng Li Ren School of Materials Science and engineering South China University of TechnologyGuangzhou510006China National engineering Research Center for tissue Restoration and Reconstruction Guangzhou510006China Key laboratory of Biomedical engineering of Guangdong Province South China University of TechnologyGuangzhou510006China Key laboratory of Biomedical Materials and engineering of the Ministry of Education South China University of TechnologyGuangzhou510006China Innovation Center for tissue Restoration and Reconstruction South China University of TechnologyGuangzhou 510006China School of Biological Science and engineering South China University of TechnologyGuangzhou510006China Sino-Singapore International Joint Research Institute Guangzhou510555China Bioland laboratory(Guangzhou Regenerative Medicine and Health Guangdong laboratory) Guangzhou510005China School of Biology and engineering Guizhou Medical UniversityGuiyangGuizhou550025China State Key laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou510623China

The damage of corneal epithelium may lead to the formation of irreversible corneal opacities and even *** migration rate of corneal epithelial cells directly affects corneal ***,we explored ocu-microRNA 24-3p(miRNA 24-3p)that can promote rabbit corneal epithelial cells migration and cornea ***,an excellent transport carrier,were exacted from adipose derived mesenchymal stem cells for loading with miRNA 24-3p to prepare miRNA 24-3p-rich exosomes(Exos-miRNA 24-3p).It can accelerate corneal epithelial migration in vitro and in *** application in cornea alkali burns,we further modified hyaluronic acid with di(ethylene glycol)monomethyl ether methacrylate(DEGMA)to obtain a thermosensitive hydrogel,also reported a thermosensitive DEGMA-modified hyaluronic acid hydrogel(THH)for the controlled release of Exos-miRNA *** formed a highly uniform and clear thin layer on the ocular surface to resist clearance from blinking and extended the drug-ocular-epithelium contact *** use of THH-3/Exos-miRNA 24-3p for 28 days after alkali burn injury accelerated corneal epithelial defect healing and epithelial *** also reduced corneal stromal fibrosis and macrophage *** 24-3p-rich exosomes functionalized DEGMA-modified hyaluronic acid hydrogel as a multilevel delivery strategy has a potential use for cell-free therapy of corneal epithelial regeneration.

关键词： miRNA 24-3p Exosome Corneal epithelium Cell migration Thermosensitive hydrogel

Inhibition of CCL2 by bindarit alleviates diabetes-associated periodontitis by suppressing inflammatory monocyte infiltration and altering macrophage properties

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Cellular & Molecular Immunology 2021年第9期18卷 2224-2235页

作者： Zongshan Shen Shuhong Kuang Min Zhang Xin Huang Jiayao Chen Meiliang Guan Wei Qin Hockin H.K.Xu Zhengmei Lin Hospital of Stomatology Guangdong Provincial Key Laboratory of StomatologyGuanghua School of StomatologySun Yat-sen UniversityGuangzhouGuangdongChina Department of Andrology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdongChina The Key laboratory for stem cells and tissue engineering Center for Stem Cell Biology and Tissue EngineeringMinistry of EducationSun Yat-sen UniversityGuangzhouGuangdongChina Department of Advanced Oral Sciences&Therapeutics University of Maryland School of DentistryBaltimoreMDUSA Center for stem Cell Biology and Regenerative Medicine University of Maryland School of MedicineBaltimoreMDUSA University of Maryland Marlene and Stewart Greenebaum Cancer Center University of Maryland School of MedicineBaltimoreMDUSA

Diabetes-associated periodontitis(DP)aggravates diabetic complications and increases mortality from *** is caused by diabetes-enhanced host immune-inflammatory responses to bacterial *** this study,we found that persistently elevated CCL2 levels in combination with proinflammatory monocyte infiltration of periodontal tissues were closely related to ***,inhibition of CCL2 by oral administration of bindarit reduced alveolar bone loss and increased periodontal epithelial thickness by suppressing periodontal ***,bindarit suppressed the infiltration of proinflammatory monocytes and altered the inflammatory properties of macrophages in the diabetic *** finding provides a basis for the development of an effective therapeutic approach for treating DP.

关键词： Diabetes-associated periodontitis bindarit proinflammatory monocytes macrophages

Assessment of infectivity and the impact on neutralizing activity of immune sera of the COVID-19 variant,CAL.20C

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2322-2324页

作者： Zhongcheng Zhou Peng Du Meixing Yu Daniel T.Baptista-Hon Man Miao Andy P.Xiang Johnson Yiu-Nam Lau COVID-Immunity Investigation Group Gen Li Kang Zhang Guangzhou Women and Children’s Medical Center Guangzhou Medical UniversityGuangzhouChina University Hospital and Center for Biomedicine and Innovations Faculty of MedicineMacao University of Science and TechnologyMacaoChina Center for stem Cell Biology and tissue engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-sen UniversityGuangzhouChina Department of Biology Hong Kong Baptist UniversityKowloon TongHong KongChina Department of Bioinformatics and Al Bioland LaboratoryGuangzhouChina

Dear Editor,New variants of SARS-CoV-2 have been emerging since the initial outbreak in 2019;1 one of the latest ones was identified in Southern California in October 2020 and was subsequently detected in 26 other sta... 详细信息

关键词： neutral impact California

Mesenchymal stem cell-derived exosomes: A novel and potential remedy for cutaneous wound healing and regeneration

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World Journal of stem cells 2022年第5期14卷 318-329页

作者： Jia-Chen Hu Chen-Xi Zheng Bing-Dong Sui Wen-Jia Liu Yan Jin Key laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi International Joint Research Center for Oral Diseases Center for Tissue EngineeringSchool of StomatologyThe Fourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina National and Local Joint engineering Research Center of Biodiagnosis and Biotherapy Precision Medicine InstituteInstitute for Stem Cell and Regenerative MedicineThe Second Affiliated HospitalXi’an Jiaotong UniversityXi’an 710032Shaanxi ProvinceChina

Poor healing of cutaneous wounds is a common medical problem in the field of *** to the intricate pathophysiological processes of wound healing,the use of conventional treatment methods,such as chemical molecule drugs and traditional dressings,have been unable to achieve satisfactory *** recent years,explicit evidence suggests that mesenchymal stem cells(MSCs)have great therapeutic potentials on skin wound healing and ***,the direct application of MSCs still faces many challenges and ***,exosomes as cell-secreted granular vesicles with a lipid bilayer membrane structure and containing specific components from the source cells may emerge to be excellent substitutes for *** derived from MSCs(MSC-exosomes)have been demonstrated to be beneficial for cutaneous wound healing and accelerate the process through a variety of *** mechanisms include alleviating inflammation,promoting vascularization,and promoting proliferation and migration of epithelial cells and ***,the application of MSC-exosomes may be a promising alternative to cell therapy in the treatment of cutaneous wounds and could promote wound healing through multiple mechanisms *** review will provide an overview of the role and the mechanisms of MSC-derived exosomes in cutaneous wound healing,and elaborate the potentials and future perspectives of MSC-exosomes application in clinical practice.

关键词： Mesenchymal stem cells Extracellular vesicles Exosomes Wound healing Skin regeneration

Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair

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Bioactive Materials 2023年第2期20卷 208-220页

作者： Qi Wu Xiaoling Fu Xian Li Jing Li Weiju Han Yingjun Wang School of Biomedical Sciences and engineering South China University of TechnologyGuangzhou International CampusGuangzhou511442PR China National engineering Research Center for tissue Restoration and Reconstruction South China University of TechnologyGuangzhou510006PR China Key laboratory of Biomedical engineering of Guangdong Province South China University of TechnologyGuangzhou510006PR China Key laboratory of Biomedical Materials and engineering of the Ministry of Education South China University of TechnologyGuangzhou510006PR China

The process of bone repair is highly regulated by a large number of bioactive ***,a“cocktail”of bioactive factors supplemented to the defect sites is desirable for bone *** this regard,small extracellular vesicles(sEVs)derived from mesenchymal stem cells hold great potential in tissue ***,the poor homing and retention of sEVs greatly limited their possible clinical *** the present work,DMPE-PEG-CREKA was inserted into the membrane of sEVs released from adipose-derived mesenchymal stem cells to obtain CREKA functionalized sEVs(CREKA-sEVs),which could target fibrin to accumulate and retain in bone *** results showed that CREKA-sEVs,like sEVs,promoted the osteogenic differentiation of BMSCs,the angiogenic property of HUVECs,and modulated the polarization of macrophages in ***,due to the improved fibrin-binding and retention capacity of CREKA-sEVs,they enhanced the bone repair substantially in the rat femoral defect *** study provided a new strategy to improve the therapeutic efficiency of sEVs and showed that CREKA-sEVs had great application value in bone tissue repair.

关键词： MSCs-derived sEVs Fibrin-targeting CREKA Bone repair

GelNB molecular coating as a biophysical barrier to isolate intestinal irritating metabolites and regulate intestinal microbial homeostasis in the treatment of inflammatory bowel disease

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Bioactive Materials 2023年第1期19卷 251-267页

作者： Qijiang Mao Haoqi Pan Yiyin Zhang Yi Zhang Qiuwen Zhu Yi Hong Zhengze Huang Yang Li Xu Feng Yifeng Fang WenChao Chen Pengfei Chen Bo Shen Hongwei Ouyang Yuelong Liang Department of General Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou310016China Key laboratory of Laparoscopic Technology of Zhejiang province Hangzhou310016China Zhejiang Province Medical Research Center of Minimally Invasive Diagnosis and Treatment of Abdominal Diseases Hangzhou310028China Dr.Li Dak Sum&Yip Yio Chin Center for stem cells and Regenerative Medicine Department of Orthopedic Surgery of the Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina Zhejiang University-University of Edinburgh Institute Zhejiang University School of MedicineKey Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang ProvinceZhejiang University School of MedicineHangzhouChina The State Key laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou310028China Department of Orthopaedic Surgery Sir Run Run Shaw HospitalMedical College of Zhejiang UniversityHangzhouZhejiang310016China

Inflammatory bowel disease(IBD)is a chronic,immune-mediated inflammatory disease characterized by the destruction of the structure and function of the intestinal epithelial *** to the poor remission effect and severe adverse events associated with current clinical medications,IBD remains an incurable ***,we demonstrated a novel treatment strategy with high safety and effective inflammation remission via tissue-adhesive molecular *** molecular coating is composed of o-nitrobenzaldehyde(NB)-modified Gelatin(GelNB),which can strongly bond with-NH_(2)on the intestinal surface of tissue to form a thin biophysical *** found that this molecular coating was able to stay on the surface of the intestine for long periods of time,effectively protecting the damaged intestinal epithelium from irritations of external intestinal metabolites and harmful *** addition,our results showed that this coating not only provided a beneficial environment for cell migration and proliferation to promote intestinal repair and regeneration,but also achieved a better outcome of IBD by reducing intestinal ***,the in vivo experiments showed that the GelNB was better than the classic clinical ***,our molecular coating showed potential as a promising strategy for the prevention and treatment of IBD.

关键词： Inflammatory bowel GelNB Molecular coating Biophysical barrier

Quantum dots-labeled polymeric scaffolds for in vivo tracking of degradation and tissue formation

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Bioactive Materials 2022年第10期7卷 285-292页

作者： Kun Hee Sim Seyed Mohammand Mir Sophia Jelke Solaiman Tarafder Jinho Kim Chang H.Lee Regenerative engineering laboratory Center for Dental and Craniofacial ResearchColumbia University Irving Medical Center630 West 168th StreetVC12-211New YorkNY10032USA Department of Biomedical engineering Stevens Institute of TechnologyHobokenNJ07030USA

The inevitable gap between in vitro and in vivo degradation rate of biomaterials has been a challenging factor in the optimal designing of scaffold’s degradation to be balanced with new tissue *** enable non-/minimum-invasive tracking of in vivo scaffold degradation,chemical modifications have been applied to label polymers with fluorescent ***,the previous approaches may have limited expandability due to complicated synthesis ***,we introduce a simple and efficient method to fluorescence labeling of polymeric scaffolds via blending with near-infrared(NIR)quantum dots(QDs),semiconductor nanocrystals with superior optical ***-labeled,3D-printed PCL scaffolds showed promising efficiency and reliability in quantitative measurement of degradation using a custom-built fiber-optic imaging ***,QDs-PCL scaffolds showed neither cytotoxicity nor secondary labeling of adjacent ***-PCL scaffolds also supported the engineering of fibrous,cartilaginous,and osteogenic tissues from mesenchymal stem/progenitor cells(MSCs).In addition,QDs-PCL enabled a distinction between newly forming tissue and the remaining mass of scaffolds through multi-channel ***,our findings suggest a simple and efficient QDs-labeling of PCL scaffolds and minimally invasive imaging modality that shows significant potential to enable in vivo tracking of scaffold degradation as well as new tissue formation.

关键词： Key terms:quantum dots Polycaprolactone tissue engineering In vivo tracking Degradation

3D bio-printed living nerve-like fibers refine the ecological niche for long-distance spinal cord injury regeneration

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Bioactive Materials 2023年第7期25卷 160-175页

作者： Jia Yang Kaiyuan Yang Weitao Man Jingchuan Zheng Zheng Cao Chun-Yi Yang Kunkoo Kim Shuhui Yang Zhaohui Hou Guihuai Wang Xiumei Wang State Key laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced MaterialsSchool of Materials Science and EngineeringTsinghua UniversityBeijing100084China Department of Neurosurgery Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing102218China School of Materials Science and engineering Zhejiang-Mauritius Joint Research Center for BiomaterialsTissue Engineering Zhejiang Sci-Tech UniversityHangzhou310018China

3D bioprinting holds great promise toward fabricating biomimetic living constructs in a bottom-up assembly *** date,various emergences of living constructs have been bioprinted for in vitro applications,while the conspicuous potential serving for in vivo implantable therapies in spinal cord injury(SCI)has been relatively ***,living nerve-like fibers are prepared via extrusion-based 3D bioprinting for SCI *** living nerve-like fibers are comprised of neural stem cells(NSCs)embedded within a designed hydrogel that mimics the extracellular matrix(ECM),assembled into a highly spatial ordered architecture,similar to densely arranged bundles of the nerve *** pro-neurogenesis ability of these living nerve-like fibers is tested in a 4 mm-long complete transected SCI rat *** shows that living nerve-like fibers refine the ecological niche of the defect site by immune modulation,angiogenesis,neurogenesis,neural relay formations,and neural circuit remodeling,leading to outstanding functional reconstruction,revealing an evolution process of this living construct after *** effective strategy,based on biomimetic living constructs,opens a new perspective on SCI therapies.

关键词： Neural stem cells Hydrogels 3D bioprinting Living constructs Spinal cord injury

Urine-derived stem cells:applications in skin,bone and articular cartilage repair

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Burns & Trauma 2021年第1期9卷 95-108页

作者： Wenqian Zhang Jungen Hu Yizhou Huang Chenyu Wu Huiqi Xie laboratory of stem Cell and tissue engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041China

As an emerging type of adult stem cell featuring non-invasive acquisition,urine-derived stem cells(USCs)have shown great potential for applications in tissue engineering and regenerative *** a growing amount of research on the topic,the effectiveness of USCs in various disease models has been shown and the underlying mechanisms have also been explored,though many aspects still remain *** this review,we aim to provide an up-to-date overview of the biological characteristics of USCs and their applications in skin,bone and articular cartilage *** addition to the identification procedure of USCs,we also summarize current knowledge of the underlying repair mechanisms and application modes of *** concerns and perspectives have also been summarized.

关键词： Urine-derived stem cells Skin Bone Articular cartilage tissue engineering Cell therapy