3D bio-printed living nerve-like fibers refine the ecological niche for long-distance spinal cord injury regeneration

作     者：Jia Yang Kaiyuan Yang Weitao Man Jingchuan Zheng Zheng Cao Chun-Yi Yang Kunkoo Kim Shuhui Yang Zhaohui Hou Guihuai Wang Xiumei Wang 

作者机构：State Key Laboratory of New Ceramics and Fine ProcessingKey Laboratory of Advanced MaterialsSchool of Materials Science and EngineeringTsinghua UniversityBeijing100084China Department of NeurosurgeryBeijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing102218China School of Materials Science and EngineeringZhejiang-Mauritius Joint Research Center for BiomaterialsTissue Engineering Zhejiang Sci-Tech UniversityHangzhou310018China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第25卷第7期

页      面：160-175页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 

基　　金：The authors thank the financial support from the National Natural Science Foundation of China(Grant No.32271414 and 82201521) the Tsinghua Precision Medicine Foundation(Grant No.2022TS001) the National Key Research and Development Program of China(Grant No.2020YFC1107600) 

主　　题：Neural stem cells Hydrogels 3D bioprinting Living constructs Spinal cord injury 

摘      要：3D bioprinting holds great promise toward fabricating biomimetic living constructs in a bottom-up assembly *** date,various emergences of living constructs have been bioprinted for in vitro applications,while the conspicuous potential serving for in vivo implantable therapies in spinal cord injury(SCI)has been relatively ***,living nerve-like fibers are prepared via extrusion-based 3D bioprinting for SCI *** living nerve-like fibers are comprised of neural stem cells(NSCs)embedded within a designed hydrogel that mimics the extracellular matrix(ECM),assembled into a highly spatial ordered architecture,similar to densely arranged bundles of the nerve *** pro-neurogenesis ability of these living nerve-like fibers is tested in a 4 mm-long complete transected SCI rat *** shows that living nerve-like fibers refine the ecological niche of the defect site by immune modulation,angiogenesis,neurogenesis,neural relay formations,and neural circuit remodeling,leading to outstanding functional reconstruction,revealing an evolution process of this living construct after *** effective strategy,based on biomimetic living constructs,opens a new perspective on SCI therapies.