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Culture of Neural stem cells in Calcium-alginate Microbeads

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生物医学工程学杂志 2005年第S1期22卷 105-106页

作者： Li-Song YAO~1 Tian-Qing LIU~(1△) Dan GE~1 Xue-Hu MA~1 Zhan-Feng CUI~2 1(stem Cell and tissue engineering laboratory, Dalian University of Technology, Dalian 116024, China)2(Department of engineering Science, Oxford University, Parks Road, Oxford OX1 3PJ, UK) stem Cell and tissue engineering laboratory Dalian University of Technology Dalian 116024 China Department of engineering Science Oxford University Parks Road Oxford OX1 3 PJ UK

1 IntroductionRecent research shows that neural stem cells may play an important role in the nerve injury reparation and nerve disease treatment. The shortage of the source and the number of NSCs, however, is the main challenge for its clinic application. In this situation, expansion of NSCs in large scale and culture in three dimensional environment are very worth of exploration. Notablely, the shear stress existed in bioreactors can cause serious cell injury especially for the shear sensitive cells like NSCs.

关键词： NSCs Ca

BAF45D regulates spinal cord neural stem/progenitor cell fate through the SMAD-PAX6 axis

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Genes & Diseases 2023年第2期10卷 366-369页

作者： Xueying Chen Xiujuan Hu Jing Jiang Jie Tao Lihua Liu Shengyun Fang Yuxian Shen Qingsong Hu Chao Liu School of Basic Medical Sciences Anhui Medical UniversityHefeiAnhui 230032China Department of Histology and Embryology Institute of Stem Cell and Tissue EngineeringAnhui Medical UniversityHefeiAnhui 230032China Division of Life Sciences and Medicine University of Science and Technology of ChinaThe First Affiliated Hospital of USTCHefeiAnhui 230001China Institute of Clinical Pharmacology Anhui Medical UniversityHefeiAnhui 230032China Center for Biomedical engineering and Technology University of MarylandBaltimoreMD 21201USA

Mutations in BRG1-associated factor 45D (BAF45D, also known as double PHD fingers 2 (DPF2)) are linked to Coffin-Siris syndrome (CSS).1 However, the underlying molecular mechanisms remain ill-defined. Here, we identified that wild-type but not CSS-associated BAF45D mutants increased the expression of PAX6,2 a neural stem cell marker, and phosphorylated SMAD3 (p-SMAD3) in spinal cord neural stem cells (NSCs) derived from H9 human embryonic stem cells. Both BAF45D and SMAD3 are required for the induction of p-SMAD3 and PAX6 together with STAT3 and SMAD7 by retinoic acid (RA). In the presence of RA, BAF45D knockdown decreased the expression of genes that regulate stem cell pluripotency. Moreover, the same genes in the spinal cord NSCs had more binding sites with BAF45D than those in H9 cells. Furthermore, Gene Ontology (GO) assay results indicated that BAF45D targets more pathways responsible for stem cell maintenance in NSCs. Finally, BAF45D is associated with BRG1, p-SMAD3, and PAX6. Together, these findings suggest a role for BAF45D in modulating PAX6 expression through SMAD signaling, highlighting the importance of BAF45D in neural stem/progenitor cell fate commitment.

关键词： PAX6 progenitor SMAD

Adult-derived human liver stem/progenitor cells as sensors of inflammation: a potential therapy for liver disorders

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Hepatobiliary Surgery and Nutrition 2019年第3期8卷 286-288页

作者： Soura Mardpour Amir Ali Hamidieh tissue engineering and Applied Cell Sciences Department School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran Department of stem cells and Developmental Biology Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECRTehranIran Pediatric stem Cell Transplant Department Children’s Medical CenterTehran University of Medical SciencesTehranIran

Advanced liver diseases remain one of the major health issues *** fibrosis,inflammatory damage,deprivation of metabolic capacity and parenchymal cell death are the main characteristics of end-stage liver *** liver transplantation has been considered as the therapy of choice in patients suffering from liver failure such as fulminant liver failure,cirrhosis and hepatocellular carcinoma(HCC).

关键词： liver damage death

MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1

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Signal Transduction and Targeted Therapy 2023年第3期8卷 1224-1236页

作者： Qiankun Li Wenzhi Hu Qilin Huang Jie Yang Bingmin Li Kui Ma Qian Wei Yaxi Wang Jianlong Su Mengli Sun Shengnan Cui Rungong Yang Haihong Li Xiaobing Fu Cuiping Zhang Department of tissue Repair and Regeneration The First Medical CenterChinese PLA General HospitalBeijing 100853China Research Center for tissue Repair and Regeneration Medical Innovation Research Department and the Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Dermatology Department The Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China PLA Key laboratory of tissue Repair and Regenerative Medicine and Beijing Key Research laboratory of Skin Injury Repair and RegenerationBeijing 100048China School of Medicine NanKai UniversityTianjing 300071China Department of Wound Repair and Dermatologic Surgery Taihe HospitalHubei University of MedicineShiyan 442000China

Unhealable diabetic wounds need to be addressed with the help of newer,more efficacious *** combined with biomaterials for sustained delivery of therapeutic agents are expected to bring new hope for chronic wound ***,the engineered exosomes modified for efficiently loading miR146a and attaching to silk fibroin patch(SFP)were demonstrated to promote diabetic wound *** fibroin binding peptide(SFBP)was screened through phage display,and SFBP-Gluc-MS2(SGM)and pac-miR146a-pac fusion protein were *** designed exosomes(SGM-Exos,miR146a-Exos,and SGM-miR146a-Exos)were isolated from the engineered placental mesenchymal stem cells(PMSCs)transduced with SGM or/and pac-miR146a-pac *** signals indicated SGM-Exo@SFP markedly increased the binding rate and the stability of ***,the loading efficiency of miR146a in SGM-miR146a-Exos was ten-fold higher than that in *** to untreated,SGM-miR146a-Exo-only treated,and SFP-only treated groups,SGM-miR146a-Exo@SFP drived wound healing associated with less inflammation,collagen deposition,and *** transcriptomics analysis suggested anti-inflammatory and regenerative effects with SGM-miR146a-Exo@SFP ***,we show efficient exosome@biomaterial-based miRNA delivery systems for regenerative medicine and tissue engineering.

关键词： miR146a wound healing

3D micropattern force triggers YAP nuclear entry by transport across nuclear pores and modulates stem cells paracrine

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National Science Review 2023年第8期10卷 180-193页

作者： Yan Li Zhenyu Zhong Cunjing Xu Xiaodan Wu Jiaqi Li Weiyong Tao Jianglin Wang Yingying Du Shengmin Zhang Advanced Biomaterials and tissue engineering Center Huazhong University of Science and Technology Department of Biomedical engineering Huazhong University of Science and Technology Research Base of Regulatory Science for Medical Devices National Medical Products Administration&Institute of Regulatory Science for Medical Devices Huazhong University of Science and Technology

Biophysical cues of the cellular microenvironment tremendously influence cell behavior by mechanotransduction. However, it is still unclear how cells sense and transduce the mechanical signals from 3D geometry to regulate cell function. Here, the mechanotransduction of human mesenchymal stem cells(MSCs) triggered by 3D micropatterns and its effect on the paracrine of MSCs are systematically investigated. Our findings show that 3D micropattern force could influence the spatial reorganization of the cytoskeleton, leading to different local forces which mediate nucleus alteration such as orientation,morphology, expression of Lamin A/C and chromatin condensation. Specifically, in the triangular prism and cuboid micropatterns, the ordered F-actin fibers are distributed over and fully transmit compressive forces to the nucleus, which results in nuclear flattening and stretching of nuclear pores, thus enhancing the nuclear import of YES-associated protein(YAP). Furthermore, the activation of YAP significantly enhances the paracrine of MSCs and upregulates the secretion of angiogenic growth factors. In contrast, the fewer compressive forces on the nucleus in cylinder and cube micropatterns cause less YAP entering the *** skin repair experiment provides the first in vivo evidence that enhanced MSCs paracrine by 3D geometry significantly promotes tissue regeneration. The current study contributes to understanding the in-depth mechanisms of mechanical signals affecting cell function and provides inspiration for innovative design of biomaterials.

关键词： bioactive materials tissue regeneration 3D micropattern force paracrine mechanotransduction

Intracellular pH-responsive iron-catechin nanoparticles with osteogenic/anti-adipogenic and immunomodulatory effects for efficient bone repair

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Nano Research 2022年第2期15卷 1153-1161页

作者： Ying Kong Feng Liu Baojin Ma Wenhan Wang Liang Li Xiaoying Xu Zhaoyang Sun Hongru Yang Yuanhua Sang Dong Li Gang Li Chao Liu Shuhua Wang Hong Liu State Key laboratory of Crystal Materials Shandong UniversityJinan 250100China Department of Periodontology School and Hospital of StomatologyCheeloo College of MedicineShandong University&Shandong Key Laboratory of Oral Tissue Regeneration&Shandong Engineering Laboratory for Dental Materials and Oral Tissue RegenerationJinan 250012China Department of Pharmacology School of Basic Medical SciencesShandong UniversityJinan 250012China Department of Pathology Jinan Women and Childrens Health HospitalJinan 250000China Department of Oral and Maxillofacial Surgery Qilu Hospital of Shandong UniversityJinan 250012China Cryomedicine laboratory Qilu Hospital of Shandong UniversityJinan 250012China Neurological Surgery Qilu Hospital of Shandong UniversityJinan 250012China Advanced Medical Research Institute Shandong UniversityJinan 250100China Institute for Advanced Interdisciplinary Research University of JinanJinan 250022China

Osteoimmunomodulation was identified as a new and important strategy to enhance osteogenic differentiation together with other osteogenic ***,approaches regulating osteogenic differentiation and macrophage polarization to remodel an osteoinductive microenvironment are separate and ***,the design and synthesis of one biomaterial that couples the osteogenic performance and immunomodulatory ability is a major challenge for efficient bone *** this study,self-assembled iron-catechin nanoparticles(Fe-cat NPs)were designed based on the coordinated reaction between iron ions and catechin and synthesized via a facile one-pot ***,Fe-cat NPs show intracellular pH-responsive disassembly and release catechin molecules under the low pH of lysosomes after *** strategy delivers catechin intracellularly and then enhances the osteogenic differentiation while inhibits the adipogenic differentiation of human adipose-derived stem cells(hADSCs).More importantly,Fe-cat NPs remodel the osteogenic immune microenvironment by resisting inflammation and promoting M2 polarization of *** a promising metal-organic nanodrug,the intracellular pH-responsive Fe-cat NPs significantly enhance the therapeutic effect of bone regneration by orchestrating osteogenic differentiation and immunomodulation,which may have great potential in bone tissue engineering.

关键词： Fe-catechin nanoparticles osteogenesis adipogenesis immunomodulation intracellular pH-responsive

3D-printed Mechanically Strong Calcium Phosphate Cement Scaffold with Metformin/stem Cell-encapsulating Alginate Microbeads for Bone tissue engineering

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Journal of Bionic engineering 2022年第6期19卷 1658-1670页

作者： Xiong Xiong Yuanyuan Chen Rupan Yuan Gengtao Qiu Michael D.Weir Hockin H.K.Xu Jin Liu Jianping Ruan Xiaofeng Chang Shuxin Qu School of Life Science and engineering Southwest Jiaotong UniversityChengdu610031China Sichuan engineering Research Center for Biomimetic Synthesis of Natural Drugs School of Life Science and EngineeringSouthwest Jiaotong UniversityChengdu610031China Key Lab of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu610031China Department of Advanced Oral Sciences and Therapeutics University of Maryland School of DentistryBaltimoreMD21201USA Center for stem Cell Biology and Regenerative Medicine University of Maryland School of MedicineBaltimoreMD21201USA University of Maryland Marlene and Stewart Greenebaum Cancer Center University of Maryland School of MedicineBaltimoreMD21201USA Key laboratory of Shannxi Province for craniofacial Precision Medicine Research Clinical Research Center of Shannxi Province for Dental and Maxillofacial DiseasesCollege of StomatologyXi’an Jiaotong UniversityXi’anShannxi710004China

The utilization of Calcium Phosphate Cement(CPC)is limited due to its low mechanical strength and difficulty to seed cells deep into the *** objectives of this study were to:(1)develop a 3D-printed CPC-dopamine-metformin scaffold encapsulating human periodontal ligament stem cells(hPDLSCs),(2)investigate the effect of dopamine on the performance of CPC,and(3)evaluate the effect of microbead degradation and metformin release on the osteogenic differentiation of the released *** mechanical property of the CPC scaffolds was elevated by adding dopamine,and the CPC scaffold with 7 wt.%dopamine had the highest compressive strength(7.35 MPa).Four types of microbeads with different content of alginate(oxidized alginate),hPDLSCs,and 2%metformin were *** and cell counting kit tests confirm that the hPDLSCs are protected by microbeads encapsulation during the CPC setting *** alkaline phosphatase test indicates that the osteogenic differentiation of hPDLSCs was enhanced by the fast release of cells and *** microbeads consisting of 2%oxidized alginate and 2%metformin were optimal for cell delivery due to favorable cell release and osteogenic *** CPC scaffold is promising used for bone regeneration in dental,craniofacial,and orthopedic applications.

关键词： Calcium phosphate cement 3D Printing Bionic Alginate microbeads Metformin

Human mesenchymal stromal cells enhance the immunomodulatory function of CD8＋CD28- regulatory T cells

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Cellular & Molecular Immunology 2015年第6期12卷 708-718页

作者： Qiuli Liu Haiqing Zheng Xiaoyong Chen Yanwen Peng Weijun Huang Xiaobo Li Gang Li Wenjie Xia Qiquan Sun an Andy Peng Xiang Cell-gene Therapy Translational Medicine Research Center The Third Affiliated Hospital Zhongshan School of Medicine Sun Yat-sen University Guangzhou Guangdong China Center for stem Cell Biology and tissue engineering The Key Laboratory for Stem Cells and Tissue Engineering Ministry of Education Sun Yat-sen University Guangzhou China Department of Rehabilitation Medicine The Third Affiliated Hospital Sun Yat-sen University Guangzhou China Institute of Blood Transfusion Guangzhou Blood Centre Guangzhou China Department of Renal Transplantation The Third Affiliated Hospital Sun Yat- sen University Guangzhou China Department of Biochemistry Zhongshan Medical School Sun Yat-sen University Guangzhou China These authors contributed enuallv tn this wnrk

One important aspect of mesenchymal stromal cells （MSCs）-mediated immunomodulation is the recruitment and induction of regulatory T （Treg） cells. However, we do not yet know whether MSCs have similar effects on the other subsets of Treg cells. Herein, we studied the effects of MSCs on CD8＋CD28- Treg cells and found that the MSCs could not only increase the proportion of CD8＋CD28- T cells, but also enhance CD8＋CD28-T cells＇ ability of hampering naive CD4＋ T-cell proliferation and activation, decreasing the production of IFN-γ by activated CD4＋ T cells and inducing the apoptosis of activated CD4＋ T cells. Mechanistically, the MSCs affected the functions of the CD8＋CD28- T cells partially through moderate upregulating the expression of IL-10 and FasL. The MSCs had no distinct effect on the shift from CD8＋CD28＋ T cells to CD8＋CD28- T cells, but did increase the proportion of CD8＋CD28- T cells by reducing their rate of apoptosis. In summary, this study shows that MSCs can enhance the regulatory function of CD8＋CD28- Treg cells, shedding new light on MSCs-mediated immune regulation.

关键词： antigens CD28 CD8-positive T cells mesenchymal stromal cells regulatory T cells

Surface Bioactive Modification of Alumina Ceramic by Mineralization in Modified SBF

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Journal of Bionic engineering 2022年第6期19卷 1637-1644页

作者： Wenmin Zhang Caixia Liang Qixuan He Xiaoyan Cao Fangli Yuan Jiandong Ye School of Material Science and engineering South China University of TechnologyGuangzhou510640China National engineering Research Center for tissue Restoration and Reconstruction Guangzhou510006China Guangdong Fapon Biotech Co.Ltd Dongguan523808China Inspection and Quarantine Comprehensive Technology Centre of Foshan Entry‐exit Inspection and Quarantine Bureau Foshan528042China Key laboratory of Biomedical Materials of Ministry of Education South China University of TechnologyGuangzhou510641China

In the present study,it is found that the prepared alumina ceramics has better mineralization ability in a newly revised simulated body *** the extension of mineralization time,the amount of hydroxyapatite(HA)crystals deposited on the surface of alumina ceramics also increased *** results of cell biological experiments of alumina ceramics with hydroxyapatite surface layer demonstrate that the mineralized materials have better biological activity and osteogenesis properties in *** the meanwhile,the ALP activity and expression of osteogenesis-related genes(OPN,ALP,Col-I,and OCN)of mouse bone marrow stromal stem cells on the samples were significantly promoted by increasing the formation of HA on the surface of alumina *** research concluded that alumina ceramics with HA phase on surface had great potential to be developed as a sort of bioactive material in the bone repair field.

关键词： Alumina Biomaterials Mineralization Hydroxyapatite Osteoblasts