MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1
作者机构：Department of Tissue Repair and RegenerationThe First Medical CenterChinese PLA General HospitalBeijing 100853China Research Center for Tissue Repair and RegenerationMedical Innovation Research Department and the Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Dermatology DepartmentThe Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Research Unit of Trauma CareTissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin InjuryRepair and RegenerationBeijing 100048China School of MedicineNanKai UniversityTianjing 300071China Department of Wound Repair and Dermatologic SurgeryTaihe HospitalHubei University of MedicineShiyan 442000China
出 版 物：《Signal Transduction and Targeted Therapy》 (信号转导与靶向治疗（英文）)
年 卷 期：2023年第8卷第3期
页 面：1224-1236页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：This work was supported by the National Nature Science Foundation of China(81901971,82172211,81830064,82172231) Natural Science Foundation of Beijing Municipal(7194316,7202197) National Key Research and Development Programs of China(2022YFA1104303) the CAMS Innovation Fund for Medical Sciences(CIFMS,2019-I2M-5-059) the Military Medical Research and Development Projects(AWS17J005,2019-126) Military Medical Science and Technology Youth Training Program(21QNPY128)
摘 要：Unhealable diabetic wounds need to be addressed with the help of newer,more efficacious *** combined with biomaterials for sustained delivery of therapeutic agents are expected to bring new hope for chronic wound ***,the engineered exosomes modified for efficiently loading miR146a and attaching to silk fibroin patch(SFP)were demonstrated to promote diabetic wound *** fibroin binding peptide(SFBP)was screened through phage display,and SFBP-Gluc-MS2(SGM)and pac-miR146a-pac fusion protein were *** designed exosomes(SGM-Exos,miR146a-Exos,and SGM-miR146a-Exos)were isolated from the engineered placental mesenchymal stem cells(PMSCs)transduced with SGM or/and pac-miR146a-pac *** signals indicated SGM-Exo@SFP markedly increased the binding rate and the stability of ***,the loading efficiency of miR146a in SGM-miR146a-Exos was ten-fold higher than that in *** to untreated,SGM-miR146a-Exo-only treated,and SFP-only treated groups,SGM-miR146a-Exo@SFP drived wound healing associated with less inflammation,collagen deposition,and *** transcriptomics analysis suggested anti-inflammatory and regenerative effects with SGM-miR146a-Exo@SFP ***,we show efficient exosome@biomaterial-based miRNA delivery systems for regenerative medicine and tissue engineering.
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