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Phytochemical Profiling and Bioactivity of A Mangrove Plant,Sonneratia apetala,from Odisha Coast of India

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Chinese Journal of Integrative Medicine 2015年第4期21卷 274-285页

作者： Jayanta Kumar Patra Swagat Kumar Das Hrudayanath Thatoi Department of biotechnology College of Engineering and TechnologyBiju Patnaik University of Technology School of biotechnology Yeungnam University

Objective： To test the antioxidant, antidiabetic, anticancer and antibacterial activities along with phytochemicals of Sonneratia apetala Buch.-Ham. Methods： The antibacterial activity was determined by agar well diffusion method. The antioxidant activity was determined by standard assay. The antidiabetic activity was evaluated by α-glucosidase inhibition assay and in vivo anticancer property was determined against Ehrlich ascites carcinoma （EAC） cells in Swiss Albino mice. Further partial characterization of the methanol extracts was carried out by thin layer chromatography, high performance liquid chromatography, 1H nuclear magnetic resonance spectroscopy and Fourier transform-infra red spectrum spectral analysis. Results： Four solvent extracts （acetone, ethanol, methanol and aqueous） of leaf and bark possess strong antioxidant properties. In vivo anticancer activity of methanol extract leaf indicated positive activity showing 34% inhibition against EAC cells in Swiss Albino mice. All extracts exhibited a-glucosidase inhibitory activity in a dose-dependent manner indicating presence of promising antidiabetic properties. The extracts possess strong antibacterial activity against the selected pathogenic bacteria （minimal inhibitory concentration ranging from 1.25-5.00 mg/mL）. The partial characterization of the methanol extracts of leaf and bark revealed the presence of phenolics as the lead compound responsible for studied bioactivities of the plant extracts. Conclusion： Sonneratia apetala extracts have potent antibacterial, antioxidant, antidiabetic and anticancer properties which can be further exploited for its pharmaceutical applications.

关键词： bioactive compounds chromatography Ehrlich ascites carcinoma mangrove Sonneratia apetala

Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis

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Fungal Diversity 2013年第1期58卷 127-141页

作者： Zhisheng Yu Baohua Zhang Wei Sun Fengli Zhang Zhiyong Li Marine biotechnology laboratory State Key Laboratory of Microbial Metabolism and School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240People’s Republic China Eastern Hepatobiliary Surgery Hospital Second Military Medical UniversityShanghai 200438People’s Republic China School of Life Sciences and biotechnology Shanghai Jiao Tong University800 Dongchuan RoadShanghai 200240People’s Republic China

Sponges are well documented to harbor large amounts of *** it is known that spongederived fungi are important sources for marine natural products,the phylogenetic diversity and biological function of sponge-associated fungi remain largely *** this study,the diversity of culturable endozoic fungi in sponges from the South China Sea was revealed based on the ITS phylogenetic *** the fungal potential for producing bioactive natural products was estimated according to the detection of Beta-ketosynthase in the polyketide synthase(PKS)gene cluster and cytotoxic activity *** a result,diverse fungi including 14 genera(Aspergillus,Penicillium,Scolecobasidium,Eurotium,Alternaria,Fusarium,Hypocreales,Yarrowia,Candida,Hypoxylon,Sporidiobolus,Schizophyllum,Bjerkandera,and Trichosporon)in ten orders(Xylariales,Moniliales,Pleosporales,Saccharomycetales,Hypocreales,Eurotiales,Sporidiobolales,Agaricales,Aphyllophorales and Tremellales)of phyla Ascomycota and Basidiomycota were isolated with Aspergillus as the predominant component in the culturable fungal ***,genera Schizophyllum,Sporidiobolus,and Bjerkandera in phylum Basidiomycota and genus Yarrowia in phylum Ascomycota were isolated from marine sponges for the first *** genes were detected in 12 isolates suggesting their potential for synthesizing PKS *** the 12 isolates with PKS genes,9 isolates displayed strong in vitro cytotoxic activity(***50<50μg/ml)against human cancer cell lines A549,Bel-7402,A-375 and *** study demonstrates the phylogenetically diverse endozoic fungi in South China Sea sponges,and highlights the potential of spongeassociated fungi in producing biologically active natural products.

关键词： Cytotoxic activity Fungal diversity Polyketide synthase(PKS)gene Sponge

Submerged vegetation removal promotes shift of dominant phytoplankton functional groups in a eutrophic lake

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Journal of Environmental Sciences 2014年第8期26卷 1699-1707页

作者： Jing Dong Kai Yang Shuangshuang Li Genbao Li Lirong Song State Key laboratory of Freshwater Ecology and biotechnology Institute of HydrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

Historical data indicate that the dominance of submerged plants in Dianchi Lake in the 1960 s was characterized by low algal density with dominance of non-toxic group J（Scenedesmus,Pediastrum,etc.）. The removal of submerged plants,which began in the 1970 s,resulted in the expansion of bloom-forming Microcystis（group M）. laboratory experiments suggested that Microcystis aeruginosa was inclined to grow and develop at elevated temperatures. The growth of Scenedesmus obliquus was slower than that of co-cultivated M. aeruginosa in the absence of Ceratophyllum demersum,especially at higher temperatures. The existence of submerged plant C. demersum could inhibit the growth of the harmful algae M. aeruginosa and this inhibitory effect by C. demersum was enhanced with an increase in temperature. Instead,with C. demersum,the growth of S. obliquus was not inhibited,but the co-cultivated M. aeruginosa was eliminated in a short time. Combined with the historical data and laboratory experiments,it was indicated that the submerged plants might play important roles in the dominance of the non-toxic group J in the historical succession. Consequently,the introduction of the submerged plant such as C. demersum might alter the dominant phytoplankton functional groups from M to J and benefit the restoration of the eutrophic lake.

关键词： Submerged vegetation Phytoplankton Functional groups

Research progress in the phytochemistry and biology of Ilex pharmaceutical resources

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Acta Pharmaceutica Sinica B 2013年第1期3卷 8-19页

作者： Dacheng Hao Xiaojie Gua Peigen Xiao Zhanguo Liang Lijia Xu Yong Peng biotechnology Institute School of Environmental and Chemical EngineeringDalian Jiaotong UniversityDalian 116028China Department of Metabolic Biology John Innes CentreNorwich Research ParkNorwich NR47UHUK Key laboratory of bioactive Substances and Resources Utilization of Chinese Herbal Medicine of Ministry of Education Institute of Medicinal Plant DevelopmentChinese Academy of Medical SciencesBeijing 100193China

Ilex is a botanical source for various health-promoting and pharmaceutically active compounds that have been used in traditional Chinese medicine and food for thousands of *** interest in Ilex pharmaceutical and food resources has led to additional discoveries of terpenoids,saponins,polyphenols(especially flavonoids),glycosides,and many other compounds in various Ilex species,and to investigation of their chemotaxonomy,molecular phylogeny and *** continuation of our studies on Ilex pharmacology and phylogeny,we review the phytochemistry,chemotaxonomy,molecular biology and phylogeny of Ilex species and their relevance to health-promotion and therapeutic *** similarity and dissimilarity between Ilex paraguariensis,the source plant of mate tea,and the source plants of large-leaved K udingcha(e.g.,Ilex kudingcha and Ilex latifolia)are *** is essential to utilize emerging technologies in non-Camellia tea studies to promote the sustainable utilization of Ilex resources and the identification and development of novel compounds with potential health and clinical *** biology and "-omics"technologies will play an increasingly important role in pharmaceutical and food research on the bioactive compounds of Ilex species.

关键词： Ilex Chemical components Pharmacology Chemotaxonomy Phylogeny Pharmaceutical and food resource

In vitro antimicrobial activities of methanolic extract from marine alga Enteromorpha intestinalis

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Asian Pacific Journal of Tropical Biomedicine 2015年第9期5卷 753-756页

作者： Ibrahim Darah Sheh-Hong Lim Industrial biotechnology Research laboratory (IBRL) School of Biological Sciences Universiti Sains Malaysia

Objective:To extract the bioactive compound from Enteromorpha intestinalis(E. intestinalis) and determine its in vitro antimicrobial activity. Methods: E. intestinalis was extracted by methanol and subjected to antimicrobial screening. The antimicrobial activity was studied by using disc diffusion and broth dilution method. The effect of the extract on the growth profile of the bacterial was also examined via time-kill assay. Microscopy observations using SEM was done to determine the major alterations in the microstructure of methicillin-resistant Staphylococcus aureus(MRSA). Results: The results showed methanolic extract of E. intestinalis exhibited a favourable antimicrobial activity against tested bacteria with produced inhibition zone ranging from 8.0-19.0 mm. However, all the tested fungi and yeast were resistant to the extract treatment. Time kill assay suggested that methanolic extract of E. intestinalis had completely inhibited MRSA growth and also exhibited prolonged antibacterial activity. The main abnormalities noted from the microscopic observations were the structural deterioration in the normal morphology and complete collapsed of the bacteria cells after 36 h of treatment. Conclusions: The significant antibacterial activity shown by crude extract suggested its potential against MRSA infection. The extract may have potential to develop as antibacterial agent in pharmaceutical use.

关键词： Antibacterial activity Enteromorpha intestinalis Marine algae Methicillin-resistant Staphylococcus aureus

bioactive phenazines from an earwig-associated Streptomyces sp.

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Chinese Journal of Natural Medicines 2019年第6期17卷 475-480页

作者： HAN Hao GUO Zhi-Kai ZHANG Bo ZHANG Mei SHI Jing LI Wei JIAO Rui-Hua TAN Ren-Xiang GE Hui-Ming State Key laboratory of Pharmaceutical biotechnology Institute of Functional BiomoleculesSchool of Life SciencesNanjing UniversityNanjing 210023China Hainan Provincial Key laboratory for Functional Components Research and Utilization of Marine Bio-resources Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101China State Key laboratory Cultivation Base for TCM Quality and Efficacy Nanjing University of Chinese MedicineNanjing 210023China

Three new phenazine-type compounds, named phenazines SA–SC(1–3), together with four new natural products(4–7), were isolated from the fermentation broth of an earwig-associated Streptomyces sp. NA04227. The structures of these compounds were determined by extensive analyses of NMR, high resolution mass spectroscopic data, as well as single-crystal X-ray diffraction measurement. Sequencing and analysis of the genome data allowed us to identify the gene cluster(spz) and propose a biosynthetic pathway for these phenazine-type compounds. Additionally, compounds 1–5 exhibited moderate inhibitory activity against acetylcholinesterase(AChE), and compound 3 showed antimicrobial activities against Micrococcus luteus.

关键词： Earwig-associated actinomycete Phenazine Biosynthetic pathway Antimicrobial activity Acetylcholinesterase inhibitory activity

Diversity and bioactivity of cultured aquatic fungi from the High Arctic region

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Advances in Polar Science 2017年第1期28卷 29-42页

作者： ZHANG Tao ZHAO Lili YU Caiyun WEI Tao YU Liyan China Pharmaceutical Culture Collection Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences& Peking Union Medical College Beijing Key laboratory of bioactive Substances and Function Food Beijing Union University

This study assessed the diversity and α-glycosidase inhibitory activity of cultured fungi isolated from four aquatic environments （stream, pond, glacial ice, and estuary） in the *** region （Svalbard, Norway, High Arctic）. A total of 134 fungal isolates were obtained from 13 water samples. Based on morphological characteristics and sequence analyses of the nuclear ribosomal DNA internal transcribed spacer region, these fungal isolates were identified as belonging to 47 species, with 26 belonging to the Ascomycota, 20 to the Basidiomycota, and one to the Zygomycota. The most frequently detected fungal species were Vishniacozyma sp. 2, Cadophora sp. 2, Phenoliferia sp. 1, Dioszegia sp. 2, and Mortierella sp.; these species occurred in 10, eight, seven, six, and five of the samples, respectively. Among the 134 fungal isolates, 17 isolates of 15 species displayed high α-glycosidase inhibitory activity in culture. The results suggest that diverse and distinct populations of cultured fungi are present in Arctic aquatic environments, and they include taxa that are potential sources of bioactive molecules that may be used as prototype drugs for medicinal proposals.

关键词： aquatic habitats bioactivity diversity environmental mycology High Arctic

Could natural phytochemicals be used to reduce nitrogen excretion and excreta‑derived N_(2)O emissions from ruminants?

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Journal of Animal Science and biotechnology 2024年第2期15卷 490-508页

作者： Yuchao Zhao Ming Liu Linshu Jiang Leluo Guan Beijing Key laboratory of Dairy Cow Nutrition College of Animal Science and TechnologyBeijing University of AgricultureBeijing 102206China College of Animal Science and Technology China Agricultural UniversityBeijing 100193China Department of Agricultural Food and Nutritional ScienceUniversity of AlbertaEdmontonAB T6G 2R3Canada

Ruminants play a critical role in our food system by converting plant biomass that humans cannot or choose not to consume into edible high-quality ***,ruminant excreta is a significant source of nitrous oxide(N_(2)O),a potent greenhouse gas with a long-term global warming potential 298 times that of carbon *** phytochemicals or forages containing phytochemicals have shown the potential to improve the efficiency of nitrogen(N)utilization and decrease N_(2)O emissions from the excreta of *** inclusion of tannins can shift more of the excreted N to the feces,alter the urinary N composition and consequently reduce N_(2)O emissions from *** oils or saponins could inhibit rumen ammonia production and decrease urinary N *** grazed pastures,large amounts of glucosinolates or aucubin can be introduced into pasture soils when animals consume plants rich in these compounds and then excrete them or their metabolites in the urine or *** inhibitory compounds are excreted in the urine,they would be directly applied to the urine patch to reduce nitrification and subsequent N_(2)O *** phytochemicals’role in sustainable ruminant production is undeniable,but much uncertainty ***,transient effects,and adverse effects limit the effectiveness of these phytochemicals for reducing N *** this review,we will identify some current phytochemicals found in feed that have the potential to manipulate ruminant N excretion or mitigate N_(2)O production and deliberate the challenges and opportunities associated with using phytochemicals or forages rich in phytochemicals as dietary strategies for reducing N excretion and excreta-derived N_(2)O emissions.

关键词： Nitrogen metabolism Nitrous oxide Plant bioactive compounds Ruminant Urine patches

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Tea and depression

Food Science and Human Wellness 2022年第3期11卷 476-482页

作者： Klaus W.Lange Yukiko Nakamura Katharina M.Lange Hui Zhao Department of Experimental Psychology University of RegensburgRegensburg 93040Germany Tianjin Key laboratory of Food and biotechnology University of CommerceTianjin 300134China

The available evidence,which derives from studies investigating mechanistic effects of tea,research on animal models as well as epidemiological studies and intervention trials in humans,suggests that compounds contained in tea may have the potential to aid in the prevention of depression or in its treatment as an addition to established therapies.A wide range of potentially antidepressive biological activities of tea components have been ***,the mechanisms of tea phytochemicals possibly capable of decreasing the risk of depressive symptoms are complex,multifaceted and not well *** pharmacological effects on circumscribed neurobiological systems may be produced not by individual tea components but rather by the synergistic action of various compounds on multiple pathophysiological mechanisms involved in *** epidemiological studies have generally demonstrated beneficial effects of tea consumption on mood and depressive symptoms,cross-sectional studies are unable to prove a cause-effect *** positive effects on mood could be firmly established,tea drinking could support mental ***,the moderate antidepressive effects observed in healthy people are not necessarily indicative of possible clinical effects in major depressive *** controlled intervention studies are needed to establish a causal relationship between bioactive compounds in tea and depression.

关键词： Depression Tea L-theanine Prevention Treatment