检索结果-南通市图书馆

Epigenetics in the modern era of crop improvements

Science China Life Sciences 2025年

作者： Yan Xue Xiaofeng Cao Xiangsong Chen Xian Deng Xingwang Deng Yong Ding Aiwu Dong Chengguo Duan Xiaofeng Fang Lei Gong Zhizhong Gong Xiaofeng Gu Chongsheng He Hang He Shengbo He Xinjian He Yan He Yuehui He Guifang Jia Danhua Jiang Jianjun Jiang Jinsheng Lai Zhaobo Lang Chenlong Li Qing Li Xingwang Li Bao Liu Bing Liu Xiao Luo Yijun Qi Weiqiang Qian Guodong Ren Qingxin Song Xianwei Song Zhixi Tian Jiawei Wang Yuan Wang Liang Wu Zhe Wu Rui Xia Jun Xiao Lin Xu Zhengyi Xu Wenhao Yan Hongchun Yang Jixian Zhai Yijing Zhang Yusheng Zhao Xuehua Zhong Daoxiu Zhou Ming Zhou Yue Zhou Bo Zhu Jiankang Zhu Qikun Liu College of Life Sciences Institute of Life Science and Green Development Hebei University Biotechnology Research Institute Chinese Academy of Agricultural Sciences College of Biology Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan Engineering and Technology Research Center of Hybrid Rapeseed Hunan University Institute of Advanced Agricultural Sciences School of Advanced Agricultural Sciences Peking University State key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University National Institute of Biological Sciences key laboratory of Seed Innovation Institute of Genetics and Developmental Biology Chinese Academy of Sciences School of Advanced Agricultural Sciences Peking-Tsinghua Center for Life Sciences Peking University Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education College of Chemistry and Molecular Engineering Peking University key laboratory of Seed Innovation State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State key laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang State key laboratory of Crop Stress Adaptation and Improvement School of Life Sciences Henan University State key laboratory of Maize Bio-breeding National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University Frontiers Science Center for Molecular Design breeding China Agricultural University Center for Crop Functional Genomics and Molecular breeding China Agricultural University Sanya Institute of China Agricultural University Hainan Yazhou Bay Seed laboratory Institute of Advan

Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades,our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Identification of a Differentially Expressed Gene PPP1CB between Porcine Longissimus dorsi of Meishan and Large White×Meishan Hybrids

引用

Acta Biochimica et Biophysica Sinica 2006年第7期38卷 450-456页

作者： Tao HUANG Yuan-Zhu XIONG Ming-Gang LEI De-Quan XU Chang-Yan DENG key laboratory of swine genetics and breeding,Ministry of Agriculture,Huazhong Agriculture University,Wuhan 430070,China key laboratory of swine genetics and breeding Ministry of Agriculture Huazhong Agriculture UniversityWuhan 430070 China

To study the molecular basis of heterosis,suppression subtractive hybridization was used toinvestigate the differences in gene expression between porcine Longissimus dorsi of F1 hybrids LargeWhite×Meishan and their female parents *** two specific subtractive cDNA libraries,the clonesselected by reverse Northern high-density blot screening were chosen to clone full-length cDNA by rapidamplification of cDNA *** expression-upregulated gene for Meishan skeletal muscle,designated proteinphosphatase 1,catalytic subunit,beta isoform(PPPICB),was *** PPPICB contains an openreading frame encoding 327 amino acid residues with 13 and 1763 nucleotides in the 5′ and 3′ untranslatedregions,respectively.A DNA fragment of 721 nucleotides was amplified and a mutation that creates/disruptsa restriction site for endonuclease RsaI was *** derived amino acid sequence of PPPICB has highhomology with the PPPICB of three species,Mus musculus(99%),human(99%)and mouse(100%).Thetissue expression analysis indicated that the swine PPP1CB gene is generally expressed in most *** role of PPP1CB and its relation to porcine heterosis are discussed.

关键词： differential gene expression pig heterosis PPP1CB suppression subtractive hybridization

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Isolation,Sequence Analysis and Expression Profile of a Novel swine Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from LandracexLarge White Cross-combination

引用

Acta Biochimica et Biophysica Sinica 2005年第3期37卷 186-191页

作者： Yong-Gang LIU Yuan-Zhu XIONG Chang-Yan DENG key laboratory of swine genetics and breeding.Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070.China key laboratory of swine genetics and breeding Ministry of Agriculture Huazhong Agricultural UniversityWuhan 430070 China

The mRNA differential display technique was performed to investigate the differences in geneexpression in the Longissimus dorsi muscle tissues from LandracexLarge White *** gene that was differentially expressed was identified using semi-quantitative reverse transcriptase-polymerase chain reaction（RT-PCR）and its complete cDNA sequence was obtained using the rapid amplifi-cation of cDNA ends（RACE）*** nucleotide sequence of the gene is not homologous to any of theknown porcine *** sequence prediction analysis revealed that the open reading frame of this geneencodes a protein of 260 amino acids that contains the putative conserved domain of the carbonic anhydrase,and this protein has high homology with the carbonic anhydrase Ⅲ（CA-Ⅲ）of four species—mouse（91%）,horse（91%）,rat（89%）and human（86%）—so that it can be defined as swine carbonic anhydrase Ⅲ.Thephylogenetic tree analysis revealed that the swine CA-Ⅲ has a closer genetic relationship with the horse CA-Ⅲthan with those of mouse,rat and *** tissue expression analysis indicated that the swine CA-Ⅲ geneis generally expressed in most *** experiment is the first to establish the primary foundation forfurther research on the swine CA-Ⅲ gene.

关键词： mRNA differential display rapid amplification of cDNA ends(RACE) novel gene carbonic anhydrase Ⅲ(CA-Ⅲ)

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular Cloning and Functional Analysis of MRLC2 Differential Expressed in Meishan×Yorkshire F1 Crossbreeds and Their Parents,Meishan Pigs

引用

Acta Biochimica et Biophysica Sinica 2006年第11期38卷 788-794页

作者： Hong-Tao XIE Ming-Gang LEI Yuan-Zhu XIONG* Chang-Yan DENG Si-Wen JIANG Bo ZUO Feng-E LI De-Quan XU Tao WANG key laboratory of swine genetics and breeding,Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China key laboratory of swine genetics and breeding Ministry of Agriculture Huazhong Agricultural UniversityWuhan 430070 China

In order to detect the molecular basis of heterosis in pigs,suppression subtractivehybridization was carried out to investigate the difference in gene expression in the Longissimus dorsimuscle tissues between Meishan×Yorkshire F1 crossbreeds and their parents,Meishan *** swinemyosin regulatory light chain 2 (MRLC2) gene differentially expressed between the crossbreeds and thepurebreds was isolated and identified using semi-quantitative reverse transcriptase polymerase chain reactionand its complete cDNA sequence was obtained using the rapid amplification of cDNA ends *** sequence of the gene is not homologous to any of the known porcine *** sequenceprediction analysis reveals that the open reading frame of this gene encodes a protein of 172 amino acidscontaining the putative conserved domain of the EF-hand *** predicted amino acid sequenceof porcine MRLC2 protein exhibits 99%,98%,98%,98% and 97% identity with that of cattle,human,dog,rat and mouse,*** homology analysis revealed that the MRLC2 protein was very muchconserved in *** tissue expression analysis indicated that the swine MRLC2 gene is highlyexpressed in muscle,fat,heart,liver,spleen,lung,kidney,stomach,small intestine,ovary and testis,but notexpressed in pancreas.

关键词： MRLC2 gene pig heterosis Longissimus dorsi suppression subtractive hybridization rapid amplification of cDNA end

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cDNA Cloning,Sequence Analysis of the Porcine LIM and Cysteine-rich Domain 1 Gene

引用

Acta Biochimica et Biophysica Sinica 2005年第12期37卷 843-850页

作者： Jun WANG Chang-Yah DENG Yuan-Zhu XIONG Bo ZUO Lei XING Feng-E LI Ming-Gang LEI Rong ZHENG Si-Wen JIANGkey Laboratiry of swine genetics and breeding,Ministry of Agriculture.College of Animal Sciences,Huazhong Agricultural University.Wuhan 430070,China key laboratory of swine genetics and breeding Ministry of Agriculture College of Animal Sciences Huazhong Agricultural UniversityWuhan 430070 China

LIM domain proteins are important regulators in cell growth,cell fate determination,celldifferentiation and remodeling of the cell cytoskeleton by their interaction with various structural proteins,kinases and transcriptional *** molecular biology combined with in silico cloning,we havecloned the complete coding sequence of pig LIM and the cysteine-rich domain 1 gene (LMCD1) whichencodes a 363 amino acid *** estimated molecular weight of the LMCD1 protein is 40,788 Da witha pI of *** was found to be highly expressed in both skeletal muscle and cardiac *** revealed that the deduced protein sequence shares 86%,91% and 93% homology with that of itshuman,mouse and rat counterparts,*** LMCD 1 protein was predicted by bioinformaticssoftware to contain a novel cysteine-rich domain in the N-terminal region,two LIM domains in theC-terminal region,nine potential protein kinase C phosphorylation sites,seven casein kinase Ⅱ phosphorylationsites,a tyrosine kinase phosphorylation site,seven N-glycosylation and N-myristoylation sites and a singlepotential N-glycosylation site,which is similar to the protein’s human *** tree wasconstructed by aligning the amino acid sequences of the LIM domain trom different *** addition,fourbase mutations were detected by comparing the sequences of Large White pigs with those of ChineseMeishan *** G294A mutation site was confirmed by polymerase chain reaction-single-strandconformation polymorphism *** allele frequencies were studied in five pig breeds.

关键词： LIM and cysteine-rich domain 1(LMCD1) SSCP PCR gene expression data analysis

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Isolation, cDNA sequence analysis and tissue expression profile of a novel swine gene differentially expressed in the Longissimus dorsi muscle tissues from Large White x Meishan cross combination

引用

Progress in Natural Science:Materials International 2005年第12期15卷 1073-1078页

作者： LIU Yonggang,LEI Minggang,XIONG Yuanzhu and DENG Changyan key laboratory of swine genetics and breeding,Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China key laboratory of swine genetics and breeding Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China

In order to study the molecular mechanism of heterosis in pigs,the mRNA differential display technique was performed to investigate the differences in gene expression in the Longissimus dorsi muscle tissues from Large White×Meishan cross combination. One novel gene differentially expressed between the hybrids and the purebreds was isolated and subsequently identified using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and its complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) *** nucleotide sequence of the gene is not homologous to any of the known porcine *** sequence prediction revealed that the open reading frame of this gene encodes a protein of 188 amino acids that contains the putative conserved domain of the PRAl family protein and this protein has high homology with the PRA1 family protein 3 of three species-rat (88% ),human (88%),and mouse (87%),-so that it can be defined as swine PRA1 family protein *** phylogenetic tree analysis revealed that the swine PRA1 family protein 3 has a closer genetic relationship with the human PRA1 family protein 3 than with those of mouse and rat. The tissue expression analysis indicated that swine PRA1family protein 3 gene is highly-expressed in muscle and fat,moderately in spleen, weakly in heart,kidney,ovary,lung,and almost not expressed in small intestine and *** function of this gene and the relationship between this gene and heterosis are also discussed.

关键词： pig heterosis Longissimus dorsi mRNA differential display RACE PRA1 family protein 3

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits

引用

Journal of Integrative Agriculture 2013年第1期12卷 103-109页

作者： NIU Bu-yue LAN Xiao-ming YAN Xiao-hong DI Sheng-wei WANG Yang LI Hai-tao XIONG Yuan- zhu WANG Xi-biao College of Animal Science and Technology Northeast Agricultural University key laboratory of swine genetics and breeding Ministry of Agriculture/Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction Ministry of Education/Huazhong Agricultural University

The matrix metalloproteinase 23 （MMP-23）, which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 （pMMP-23） gene, including exons 2-8 （GenBank： EU360790）, was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 （EU360790： g. 269C〉T） and an A/G mutation in exon 4 （EU360790： g. 505A〉G）, respectively. Association study for these two SNPs with litter size was assessed in three independent populations （Minzhu, Landrace I and Landrace II）. Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed （P〈0.05） at g. 269C〉T locus, and the additive effect was significant （P〈0.05）; GG sows produced more TNB and NBA than AA pigs in Minzhu （P〈0.01） and Landrace breeds （P〈0.05） at g. 505A〉G locus, and the additive effect was significant （P〈0.01 or P〈0.05）. Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A〉G locus might be a useful molecular marker for marker assisted selection （MAS）.

关键词： pig MMP-23 molecular cloning expression litter size

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and Their Parents

引用

Acta Biochimica et Biophysica Sinica 2006年第11期38卷 753-758页

作者： Zhu-Qing REN Yuan-Zhu XIONG* Chang-Yan DENG Ming-Gang LEI Agricultural Ministry key laboratory of swine breeding and genetics,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China Agricultural Ministry key laboratory of swine breeding and genetics College of Animal Science and Technology Huazhong Agricultural UniversityWuhan 430070 China

In order to investigate porcine heterosis on the molecular basis,Large White (L),a Europeanpurebred,and Meishan (M),a Chinese indigenous purebred,were hybridized directly and reciprocally toproduce F1 hybrids,Large White×Meishan (LM) and Meishan×Large White (ML) *** mRNAdifferential display,we found an expression sequence tag (EST) differentially expressed in F1 hybrids andtheir parents,designated as EST55,which was homologous to human and murine skeletal muscle protein(SMPX),and the full-length cDNA of porcine SMPX was cloned by the rapid amplification of cDNA end(RACE) *** of the mRNA transcript revealed an open reading frame (ORF) of 86 aminoacid residues encoding a nuclear location signal peptide,two overlapping casein kinase Ⅱ phosphorylationsites and one N-glycosylation site with theoretical molecular weight of 9.3 *** analysis revealedthat the deduced protein sequence shared 94%,83% and 78% homology with that of its human,mouse andrat counterparts,*** transcription-polymerase chain reaction (RT-PCR) analysis showedthat it was expressed predominantly in skeletal and heart muscles,whereas at a moderate level in backfat,spleen,stomach and uterus *** single nucleotide polymorphism (SNPs),located in 5′- and 3′-untranslated region (UTR),respectively,were identified by PCR and *** tree and thesecondary structure prediction were also *** possible relationship between porcine SMPX andheterosis was discussed.

关键词： skeletal muscle protein pig mRNA differential display expression pattern

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Whole genome variants across 57 pig breeds enable comprehensive identification of genetic signatures that underlie breed features

引用

Journal of Animal Science and Biotechnology 2021年第2期12卷 492-507页

作者： Jingya Xu Yuhua Fu Yan Hu Lilin Yin Zhenshuang Tang Dong Yin Mengjin Zhu Mei Yu Xinyun Li Yang Zhou Shuhong Zhao Xiaolei Liu key laboratory of Agricultural Animal genetics Breeding and ReproductionMinistry of Education&Key Laboratory of Swine Genetics and BreedingMinistry of Agriculture&College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiPR China

Background:A large number of pig breeds are distributed around the world,their features and characteristics vary among breeds,and they are valuable *** the underlying genetic mechanisms that explain across-breed variation can help breeders develop improved pig ***:In this study,we performed GWAS using a standard mixed linear model with three types of genome variants(SNP,InDel,and CNV)that were identified from public,whole-genome,sequencing data *** used 469 pigs of 57 breeds,and we identified and analyzed approximately 19 million SNPs,1.8 million InDels,and 18,016 *** defined six biological phenotypes by the characteristics of breed features to identify the associated genome variants and candidate genes,which included coat color,ear shape,gradient zone,body weight,body length,and body height.A total of 37 candidate genes was identified,which included 27 that were reported previously(e.g.,PLAG1 for body weight),but the other 10 were newly detected candidate genes(e.g.,ADAMTS9 for coat color).Conclusion:Our study indicated that using GWAS across a modest number of breeds with high density genome variants provided efficient mapping of complex traits.

关键词： Breed feature CNV GWAS InDel Pig SNP

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A comprehensive evaluation of factors affecting the accuracy of pig genotype imputation using a single or multi-breed reference population

引用

Journal of Integrative Agriculture 2022年第2期21卷 486-495页

作者： ZHANG Kai-li PENG Xia ZHANG Sai-xian ZHAN Hui-wen LU Jia-hui XIE Sheng-song ZHAO Shu-hong LI Xin-yun MA Yun-long key laboratory of Agricultural Animal genetics Breeding and ReproductionMinistry of Education/Key Laboratory of Swine Genetics and BreedingMinistry of Agriculture and Rural Affairs/College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070P.R.China

Genotype imputation has become an indispensable part of genomic data analysis. In recent years, imputation based on a multi-breed reference population has received more attention, but the relevant studies are scarce in pigs. In this study, we used the Illumina Porcine SNP50 Bead Chip to investigate the variations of imputation accuracy with various influencing factors and compared the imputation performance of four commonly used imputation software programs. The results indicated that imputation accuracy increased as either the validation population marker density, reference population sample size, or minor allele frequency(MAF) increased. However, the imputation accuracy would have a certain extent of decrease when the pig reference population was a mixed group of multiple breeds or lines. Considering both imputation accuracy and running time, Beagle 4.1 and FImpute are excellent choices among the four software packages tested. This work visually presents the impacts of these influencing factors on imputation and provides a reference for formulating reasonable imputation strategies in actual pig breeding.

关键词： genotype imputation multi-breed reference population imputation accuracy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：