Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and Their Parents
Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and Their Parents作者机构：Agricultural Ministry Key Laboratory of Swine Breeding and Genetics College of Animal Science and Technology Huazhong Agricultural UniversityWuhan 430070 China
出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))
年 卷 期：2006年第38卷第11期
页 面：753-758页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0905[农学-畜牧学] 09[农学] 0703[理学-化学]
主 题：skeletal muscle protein pig mRNA differential display expression pattern
摘 要：In order to investigate porcine heterosis on the molecular basis,Large White (L),a Europeanpurebred,and Meishan (M),a Chinese indigenous purebred,were hybridized directly and reciprocally toproduce F1 hybrids,Large White×Meishan (LM) and Meishan×Large White (ML) *** mRNAdifferential display,we found an expression sequence tag (EST) differentially expressed in F1 hybrids andtheir parents,designated as EST55,which was homologous to human and murine skeletal muscle protein(SMPX),and the full-length cDNA of porcine SMPX was cloned by the rapid amplification of cDNA end(RACE) *** of the mRNA transcript revealed an open reading frame (ORF) of 86 aminoacid residues encoding a nuclear location signal peptide,two overlapping casein kinase Ⅱ phosphorylationsites and one N-glycosylation site with theoretical molecular weight of 9.3 *** analysis revealedthat the deduced protein sequence shared 94%,83% and 78% homology with that of its human,mouse andrat counterparts,*** transcription-polymerase chain reaction (RT-PCR) analysis showedthat it was expressed predominantly in skeletal and heart muscles,whereas at a moderate level in backfat,spleen,stomach and uterus *** single nucleotide polymorphism (SNPs),located in 5′- and 3′-untranslated region (UTR),respectively,were identified by PCR and *** tree and thesecondary structure prediction were also *** possible relationship between porcine SMPX andheterosis was discussed.
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