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18β-glycyrrhetinic acid promotes gastric cancer cell autophagy and inhibits proliferation by regulating miR-328-3p/signal transducer and activator of transcription 3

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World Journal of Gastroenterology 2023年第27期29卷 4317-4333页

作者： Yi Yang Yi Nan Yu-Hua Du Shi-Cong Huang Dou-Dou Lu Jun-Fei Zhang Xia Li Yan Chen Lei Zhang Ling Yuan ningxia Medical University Yinchuan 750004Ningxia Hui Autonomous RegionChina key laboratory of ningxia minority medicine modernization Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Gastric cancer(GC)is one of the most common cancer types worldwide,and its prevention and treatment methods have garnered much *** the active ingredient of licorice,18β-glycyrrhetinic acid(18β-GRA)has a variety of pharmacological *** aim of this study was to explore the effective target of 18β-GRA in the treatment of GC,in order to provide effective ideas for the clinical prevention and treatment of *** To investigate the mechanism of 18β-GRA in inhibiting cell proliferation and promoting autophagy flux in GC *** Whole transcriptomic analyses were used to analyze and screen differentially expressed microRNAs(miRNAs)in GC cells after 18β-GRA ***-transfected GC cells and the Cell Counting Kit-8 were used to detect cell proliferation ability,cell colony formation ability was detected by the clone formation assay,and flow cytometry was used to detect the cell cycle and apoptosis.A nude mouse transplantation tumor model of GC cells was constructed to verify the effect of miR-328-3p overexpression on the tumorigenicity of GC *** tissue morphology was observed by hematoxylin and eosin staining,and microtubule-associated protein light chain 3(LC3)expression was detected by ***,STRING,and miRWalk databases were used to predict the relationship between miR-328-3p and signal transducer and activator of transcription 3(STAT3)-related *** of STAT3 mRNA and miR-328-3p was detected by quantitative polymerase chain reaction(qPCR)and the expression levels of STAT3,phosphorylated STAT3(p-STAT3),and LC3 were detected by western blot *** targeted relationship between miR-328-3p and STAT3 was detected using the dual-luciferase reporter gene *** cells were infected with monomeric red fluorescent protein-green fluorescent protein-LC3 adenovirus double ***3 was labeled and autophagy flow was observed under a confocal laser *** The expression

关键词： 18β-glycyrrhetinic acid miR-328-3p Signal transducer and activator of transcription 3 Cell proliferation Autophagy flow

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Antibacterial Activity of Plants in Cirsium:A Comprehensive Review

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Chinese Journal of Integrative medicine 2024年第9期30卷 835-841页

作者： TANG Xiao-meng XIE Ming-xia GOU Jun-li CHEN Liang TIAN Jin-long ZHANG Xia LU You-yuan WANG Han-qing College of Pharmacy Ningxia Medical UniversityYinchuanNingxia750004China College of Traditional Chinese medicine Hunan University of Chinese MedicineChangsha410007China General Hospital of ningxia Medical University YinchuanNingxia750004China ningxia Regional Characteristic Traditional Chinese medicine Collaborative Innovation Center Co-constructed by the Province and Ministry Ningxia Engineering and Technology Research Center for Modernization of Regional Characteristic Traditional Chinese MedicineNingxia Medical UniversityYinchuan750004China key laboratory of ningxia minority medicine modernization Ministry of EducationYinchuan750004China

As ethnic medicine,the whole grass of plants in Cirsium was used as *** review focuses on the antimicrobial activity of plants in Cirsium,including antimicrobial components,against different types of microbes and bacteriostatic *** results showed that the main antimicrobial activity components in Cirsium plants were flavonoids,triterpenoids and phenolic acids,and the antimicrobial ability varied according to the species and the content of *** them,phenolic acids showed a strong antibacterial ability against Acinetobacter baumannii,Pseudomonas aeruginosa,and Enterococcus *** antibacterial mechanisms include:(1)damaging the cell membrane,cell walls,mitochondria and nucleus of bacteria;(2)inhibiting the synthesis of proteins and nucleic acids;(3)suppressing the synthesis of enzymes for tricarboxylic acid cycle pathways and glycolysis,and then killing the bacteria via inhibition of energy ***,most research results on antimicrobial activity of Cirsium plants are reported based on in vitro *** evidence from clinical data and comprehensive evaluation are needed.

关键词： Cirsium traditional use antibacterial active components bacteriostatic activity bacteriostatic mechanism Chinese medicine

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Using Network Pharmacology and Molecular Docking Tools to Investigate the Potential Mechanism of Ephedra-Gypsum in the Treatment of Respiratory Diseases

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World Journal of Traditional Chinese medicine 2023年第2期9卷 150-159页

作者： Can Huang Ling Yuan Yang Niu Ya-Ting Yang Yi-Fan Yang Yi Nan Hong-Li Dou Joanna Japhet School of Chinese medicine Ningxia Medical UniversityYinchuanNingxiaChina Pharmacy College of ningxia Medical University YinchuanNingxiaChina key laboratory of ningxia minority medicine modernization Ministry of Education Ningxia Medical UniversityYinchuanNingxiaChina School of Marxism Shaanxi University of ChineseMedicineXianyangShaanxiChina

Objective:The objective of this study was to investigate the potential mechanisms of ephedra-gypsum in the treatment ofrespiratory diseases(RDs)using network pharmacology and molecular docking *** and Methods:The TCMSP and UniProt databases were used to mine the active components and targets of ephedra-gypsum,and the targets of RD were screened using the Online Mendelian Inheritance in Man(OMIM)and GeneCards *** protein-protein interaction network graph was created using the drug-disease intersection targets in the STRING *** network diagram was analyzed using Cytoscape 3.9.1's topology *** gene ontology(GO)and KEGG enrichment analyses were performed using the DAVID *** docking bioactivity validation of the main active components and core targets was performed using AutoDock and PyMOL ***:Twenty-four compounds were screened,and 113 drug-disease targets *** total,358 biological processes,67 molecular functions,38 cellular components of GO,and 139 pathways were *** docking analysis demonstrated the strong binding ability of tumor protein 53(TP53)-***:The core components of ephedra-gypsum,such as quercetin,luteolin,kaempferol,and CaSO_(4)·2H_(2)O,act on key targets,such as tumor necrosis factor(TNF),interleukin-6(IL-6),TP53,and IL-1β through cytokine-mediated signaling pathways,infiammatory responses,cell proliferation,and *** could be useful for the treatment of RD.

关键词： Ephedra-gypsum mechanistic studies molecular docking network pharmacology respiratory diseases

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Mechanism of pachymic acid in the treatment of gastric cancer based on network pharmacology and experimental verification

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World Journal of Gastrointestinal Oncology 2024年第1期16卷 30-50页

作者： Yu-Hua Du Jian-Jun Zhao Xia Li Shi-Cong Huang Na Ning Guo-Qing Chen Yi Yang Yi Nan Ling Yuan College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina ningxia Chinese medicine Research Center Manufacturing LaboratoryYinchuan 750004Ningxia Hui Autonomous RegionChina College of Foundation Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina key laboratory of ningxia minority medicine modernization Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Pachymic acid(PA)is derived from Poria *** has a variety of pharmacological and inhibitory effects on various ***,the mechanism of action of PA in gastric cancer(GC)remains *** To investigate the mechanism of PA in treating GC via the combination of network pharmacology and experimental *** The GeneCards and OMIM databases were used to derive the GC targets,while the Pharm Mapper database provided the PA *** the STRING database,a protein-protein interaction network was constructed and core targets were *** analyses of Gene Ontology,Kyoto Encyclopedia of Genes and Genomes(KEGG),and gene set enrichment analysis were conducted,and molecular docking and clinical correlation analyses were performed on the core ***,the network pharmacology findings were validated through in vitro cell assays,encompassing assessments of cell viability,apoptosis,cell cycle,cloning,and western blot *** According to network pharmacology analysis,the core targets were screened,and the PI3K/AKT signaling pathway is likely to be the mechanism by which PA effectively treats GC,according to KEGG enrichment *** experimental findings showed that PA could control PI3K/AKT signaling to prevent GC cell proliferation,induce apoptosis,and pause the cell *** Network pharmacology demonstrated that PA could treat GC by controlling a variety of signaling pathways and acting on a variety of *** has also been supported by in vitro cell studies,which serve as benchmarks for further research.

关键词： Pachymic acid Gastric cancer Network pharmacology Enrichment analysis Cell proliferation

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Molecular mechanisms of Buqing granule for the treatment of diabetic retinopathy:Network pharmacology analysis and experimental validation

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World Journal of Diabetes 2024年第9期15卷 1942-1961页

作者： Yi-Fan Yang Ling Yuan Xiang-Yang Li Qian Liu Wen-Jie Jiang Tai-Qiang Jiao Jia-Qing Li Meng-Yi Ye Yang Niu Yi Nan key laboratory of ningxia minority medicine modernization Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese medicine College Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina School of Clinical medicine Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Diabetic retinopathy(DR)is a common microvascular complication of diabetes *** blindness rate is high;therefore,finding a reasonable and safe treatment plan to prevent and control DR is ***,there are abundant and diverse research results on the treatment of DR by Chinese medicine Traditional Chinese medicine compounds are potentially advantageous for DR prevention and treatment because of its safe and effective therapeutic *** To investigate the effects of Buqing granule(BQKL)on DR and its mechanism from a systemic perspective and at the molecular level by combining network pharmacology and in vivo *** This study collected information on the drug targets of BQKL and the therapeutic targets of DR for intersecting target gene analysis and protein-protein interactions(PPI),identified various biological pathways related to DR treatment by BQKL through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses,and preliminarily validated the screened core targets by molecular ***,we constructed a diabetic rat model with a high-fat and high-sugar diet and intraperitoneal streptozotocin injection,and administered the appropriate drugs for 12 weeks after the model was successfully *** mass and fasting blood glucose and lipid levels were measured,and pathological changes in retinal tissue were detected by hematoxylin and eosin *** was used to detect the oxidative stress index expression in serum and retinal tissue,and immunohistochemistry,real-time quantitative reverse transcription PCR,and western blotting were used to verify the changes in the expression of core *** Six potential therapeutic targets of BQKL for DR treatment,including Caspase-3,c-Jun,TP53,AKT1,MAPK1,and MAPK3,were screened using *** analysis indicated that the MAPK signaling pathway might be the core target pathway of BQKL in DR *** docking prediction indica

关键词： Diabetic retinopathy Network pharmacology Animal models Oxidative stress Inflammatory

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Network pharmacology study and in vitro experimental validation of Xiaojianzhong decoction against gastric cancer

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World Journal of Gastrointestinal Oncology 2024年第9期16卷 3932-3954页

作者： Guo-Qing Chen Yi Nan Na Ning Shi-Cong Huang Yu-Ting Bai Zi-Ying Zhou Gu Qian Wei-Qiang Li Ling Yuan College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina key laboratory of ningxia minority medicine modernization Ministry of EducationNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of Pharmacy Ningxia Chinese Medicine Research CenterYinchuan 750021Ningxia Hui Autonomous RegionChina Department of Pharmacy General Hospital of Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of Chinese Medical Gastroenterology The Affiliated TCM Hospital of Ningxia Medical UniversityWuzhong 751100Ningxia Hui Autonomous RegionChina

BACKGROUND Cancer is one of the most serious threats to human health *** treatments such as surgery and chemotherapy are associated with some *** recent years,traditional Chinese medicine treatment has been increasingly advocated by patients and attracted attention from clinicians,and has become an indispensable part of the comprehensive treatment for gastric *** To investigate the mechanism of Xiaojianzhong decoction(XJZ)in the treatment of gastric cancer(GC)by utilizing network pharmacology and experimental validation,so as to provide a theoretical basis for later experimental *** We analyzed the mechanism and targets of XJZ in the treatment of GC through network pharmacology and ***,we verified the impact of XJZ treatment on the proliferative ability of GC cells through CCK-8,apoptosis,cell cycle,and clone formation ***,we performed Western blot analysis and real-time quantitative PCR to assess the protein and mRNA expression of the core *** XJZ mainly regulates IL6,PTGS2,CCL2,MMP9,MMP2,HMOX1,and other target genes and pathways in cancer to treat *** inhibition of cell viability,the increase of apoptosis,the blockage of the cell cycle at the G0/G1 phase,and the inhibition of the ability of cell clone formation were observed in AGS and HGC-27 cells after XJZ *** addition,XJZ induced a decrease in the mRNA expression of IL6,PTGS2,MMP9,MMP2,and CCL2,and an increase in the mRNA expression of *** significantly inhibited the expression of IL6,PTGS2,MMP9,MMP2,and CCL2 proteins and promoted the expression of the heme oxygenase-1 *** XJZ exerts therapeutic effects against GC through multiple components,multiple targets,and multiple *** findings provide a new idea and scientific basis for further research on the molecular mechanisms underlying the therapeutic effects of XJZ in the treatment of GC.

关键词： Xiaojianzhong decoction Gastric cancer Network pharmacology Molecular mechanism In vitro experiment

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To explore the mechanism of Yigong San anti-gastric cancer and immune regulation

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 1965-1994页

作者： Dou-Dou Lu Ling Yuan Zhao-Zhao Wang Jian-Jun Zhao Yu-Hua Du Na Ning Guo-Qing Chen Shi-Cong Huang Yi Yang Zhe Zhang Yi Nan School of Clinical medicine Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese medicine College Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of Chinese Medical Gastrointestinal China-Japan Friendship HospitalBeijing 100029China key laboratory of ningxia minority medicine modernization Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Yigong San(YGS)is a representative prescription for the treatment of digestive disorders,which has been used in clinic for more than 1000 ***,the mechanism of its anti-gastric cancer and regulate immunity are still remains *** To explore the mechanism of YGS anti-gastric cancer and immune *** Firstly,collect the active ingredients and targets of YGS,and the differentially expressed genes of gastric ***,constructed a protein-protein interaction network between the targets of drugs and diseases,and screened hub *** the clinical relevance,mutation and repair,tumor microenvironment and drug sensitivity of the hub gene were ***,molecular docking was used to verify the binding ability of YGS active ingredient and hub *** Firstly,obtained 55 common targets of gastric cancer and *** Kyoto Encyclopedia of Genes and Genomes screened the microtubule-associated protein kinase signaling axis as the key pathway and IL6,EGFR,MMP2,MMP9 and TGFB1 as the hub *** 5 hub genes were involved in gastric carcinogenesis,staging,typing and prognosis,and their mutations promote gastric cancer ***,molecular docking results confirmed that the components of YGS can effectively bind to therapeutic *** YGS has the effect of anti-gastric cancer and immune regulation.

关键词： Gastric cancer Yigong San Mechanism Immune

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Potential application of Nardostachyos Radix et Rhizoma-Rhubarb for the treatment of diabetic kidney disease based on network pharmacology and cell culture experimental verification

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World Journal of Diabetes 2024年第3期15卷 530-551页

作者： Meng-Ying Che Ling Yuan Jiao Min Duo-Jie Xu Dou-Dou Lu Wen-Jing Liu Kai-Li Wang Yan-Yan Wang Yi Nan key laboratory of ningxia minority medicine modernization Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina College of Pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese medicine College Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina School of Clinical medicine Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of Endocrinology Yinchuan Hospital of Traditional Chinese MedicineYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Diabetic kidney disease(DKD)is one of the serious complications of diabetes mellitus,and the existing treatments cannot meet the needs of today's *** Chinese medicine has been validated for its efficacy in DKD after many years of clinical ***,the specific mechanism by which it works is still *** the molecular mechanism of the Nardostachyos Radix et Rhizoma-rhubarb drug pair(NRDP)for the treatment of DKD will provide a new way of thinking for the research and development of new *** To investigate the mechanism of the NRDP in DKD by network pharmacology combined with molecular docking,and then verify the initial findings by in vitro *** The Traditional Chinese medicine Systems Pharmacology(TCMSP)database was used to screen active ingredient targets of *** for DKD were obtained based on the Genecards,OMIM,and TTD *** VENNY 2.1 database was used to obtain DKD and NRDP intersection targets and their Venn diagram,and Cytoscape 3.9.0 was used to build a"drug-component-target-disease"*** String database was used to construct protein interaction *** Encyclopedia of Genes and Genomes(KEGG)enrichment analysis and Gene Ontology analysis were performed based on the DAVID *** selecting the targets and the active ingredients,Autodock software was used to perform molecular *** experimental validation using renal tubular epithelial cells(TCMK-1),we used the Cell Counting Kit-8 assay to detect the effect of NRDP on cell viability,with glucose solution used to mimic a hyperglycemic *** cytometry was used to detect the cell cycle progression and *** blot was used to detect the protein expression of STAT3,p-STAT3,BAX,BCL-2,Caspase9,and *** A total of 10 active ingredients and 85 targets with 111 disease-related signaling pathways were obtained for *** analysis of KEGG pathways was performed to determ

关键词： Nardostachyos Radix et Rhizoma-rhubarb Diabetic kidney disease Molecular docking Network pharmacology Experimental validation

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A new strategy:Inhibitory effect and mechanism of Cymbopogon martinii essential oil on Aspergillus flavus

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Food Bioscience 2023年第1期51卷 967-975页

作者： Lizhen Lin Shuaipeng Chen Wenxin Xia Tingting Li Lin Dong Yan Zhang Yuanyuan Zhang Hangying Li Yunsheng Zhao Xueyan Fu School of Pharmacy Ningxia Medical University750004YinchuanChina key laboratory of ningxia Ethnomedicine modernization Ministry of EducationNingxia Medical University750004YinchuanChina key laboratory of Integrated Traditional Chinese and Western medicine for Prevention Treatment of High Incidence in Ningxia Hui Autonomous Region750004YinchuanChina School of Pharmacy Hebei University of Chinese Medicine050091ShijiazhuangChina

Cymbopogon martinii(***)essential oil can inhibit the growth and toxin synthesis of Aspergillus flavus(***).In this study,*** essential oil effectively inhibited radial growth,spore production,mycelium formation and aflatoxin B1(AFB1)synthesis of *** also significantly changed the morphology and ultrastructure of *** essential oil significantly changed the activity of antioxidant enzymes and lipid peroxidation level in mycelium,and ROS level increased *** to proteomic analysis,1604 membrane proteins of *** have been *** essential oil treatment seriously damaged the integrity of the *** cell membrane,and *** essential oil treatment has destroyed 58 cell membrane proteins and released 157 membrane *** study provided a new explanation for the inhibition mechanism of *** essential oil on ***,and also provided technical support for the application of *** essential oil in mildew prevention.

关键词： Aspergillus flavus Cymbopogon martinii essential Oil Antifungal mechanism Oxidative damage Cell membrane damage

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Selective aerobic oxidation of p-cresol with co-catalysts between metalloporphyrins and metal salts

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Chinese Journal of Chemical Engineering 2018年第7期26卷 1493-1498页

作者： Yuanbin She Yao Fan Lei Zhang Ying Xu Min Yu Haiyan Fu State key laboratory Breeding Base of Green Chemistry-Synthesis Technology College of Chemical Engineering Zhejiang University of Technology The modernization Engineering Technology Research Center of Ethnic minority medicine of Hubei Province School of Pharmaceutical Sciences South Central University for Nationalities

A green and efficient method for the selective aerobic oxidation of p-cresol to p-hydroxybenzaldehyde catalyzed by co-catalysts between metalloporphyrins and metal salts was investigated and developed. The relationship between the synergistic catalytic effects and the composition as well as amount of co-catalysts was investigated. Moreover, the influence of different reaction conditions was studied in details. A high p-cresol conversion （〉99%） and p-hydroxybenzaldehyde selectivity （83%） were obtained using only 1.125 × 10- 5 mol T（p-CH3O）PPFe111Cl-Co（OAc）2 used under mild, optimized reaction conditions. A possible mechanism for the reaction was also proposed. This work would be meaningful and instructive for the further researches and applications of co-catalyst system on oxidation of cresols and could give some enlightenment on the selectively catalytic oxidation of the side-chain alkyls of aromatics.

关键词： Aerobic Oxidation Selectivity Co-catalyst Metalloporphyrin Cresols

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