Molecular mechanisms of Buqing granule for the treatment of diabetic retinopathy:Network pharmacology analysis and experimental validation
作者机构：Key Laboratory of Ningxia Minority Medicine Modernization Ministry of EducationNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina College of PharmacyNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese Medicine CollegeNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina School of Clinical MedicineNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina
出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)
年 卷 期：2024年第15卷第9期
页 面：1942-1961页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100212[医学-眼科学] 10[医学]
基 金：Supported by National Natural Science Foundation of China,No.81960836 Ningxia Natural Science Foundation,No.2020AAC03126 Ningxia Higher Education Scientific Research Project,No.NGY2020045
主 题：Diabetic retinopathy Network pharmacology Animal models Oxidative stress Inflammatory
摘 要：BACKGROUND Diabetic retinopathy(DR)is a common microvascular complication of diabetes *** blindness rate is high;therefore,finding a reasonable and safe treatment plan to prevent and control DR is ***,there are abundant and diverse research results on the treatment of DR by Chinese medicine Traditional Chinese medicine compounds are potentially advantageous for DR prevention and treatment because of its safe and effective therapeutic *** To investigate the effects of Buqing granule(BQKL)on DR and its mechanism from a systemic perspective and at the molecular level by combining network pharmacology and in vivo *** This study collected information on the drug targets of BQKL and the therapeutic targets of DR for intersecting target gene analysis and protein-protein interactions(PPI),identified various biological pathways related to DR treatment by BQKL through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses,and preliminarily validated the screened core targets by molecular ***,we constructed a diabetic rat model with a high-fat and high-sugar diet and intraperitoneal streptozotocin injection,and administered the appropriate drugs for 12 weeks after the model was successfully *** mass and fasting blood glucose and lipid levels were measured,and pathological changes in retinal tissue were detected by hematoxylin and eosin *** was used to detect the oxidative stress index expression in serum and retinal tissue,and immunohistochemistry,real-time quantitative reverse transcription PCR,and western blotting were used to verify the changes in the expression of core *** Six potential therapeutic targets of BQKL for DR treatment,including Caspase-3,c-Jun,TP53,AKT1,MAPK1,and MAPK3,were screened using *** analysis indicated that the MAPK signaling pathway might be the core target pathway of BQKL in DR *** docking prediction indica
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