Potential application of Nardostachyos Radix et Rhizoma-Rhubarb for the treatment of diabetic kidney disease based on network pharmacology and cell culture experimental verification

作     者：Meng-Ying Che Ling Yuan Jiao Min Duo-Jie Xu Dou-Dou Lu Wen-Jing Liu Kai-Li Wang Yan-Yan Wang Yi Nan 

作者机构：Key Laboratory of Ningxia Minority Medicine Modernization Ministry of EducationNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina College of PharmacyNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Traditional Chinese Medicine CollegeNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina School of Clinical MedicineNingxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Department of EndocrinologyYinchuan Hospital of Traditional Chinese MedicineYinchuan 750004Ningxia Hui Autonomous RegionChina 

出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)

年 卷 期：2024年第15卷第3期

页      面：530-551页

核心收录：

学科分类：1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China,No.81573695,No.81860894,and No.81674096 Ningxia Key Research and Development Plan Project,No.2021BEG03106 

主　　题：Nardostachyos Radix et Rhizoma-rhubarb Diabetic kidney disease Molecular docking Network pharmacology Experimental validation 

摘      要：BACKGROUND Diabetic kidney disease(DKD)is one of the serious complications of diabetes mellitus,and the existing treatments cannot meet the needs of today s *** Chinese medicine has been validated for its efficacy in DKD after many years of clinical ***,the specific mechanism by which it works is still *** the molecular mechanism of the Nardostachyos Radix et Rhizoma-rhubarb drug pair(NRDP)for the treatment of DKD will provide a new way of thinking for the research and development of new *** To investigate the mechanism of the NRDP in DKD by network pharmacology combined with molecular docking,and then verify the initial findings by in vitro *** The Traditional Chinese Medicine Systems Pharmacology(TCMSP)database was used to screen active ingredient targets of *** for DKD were obtained based on the Genecards,OMIM,and TTD *** VENNY 2.1 database was used to obtain DKD and NRDP intersection targets and their Venn diagram,and Cytoscape 3.9.0 was used to build adrug-component-target-disease*** String database was used to construct protein interaction *** Encyclopedia of Genes and Genomes(KEGG)enrichment analysis and Gene Ontology analysis were performed based on the DAVID *** selecting the targets and the active ingredients,Autodock software was used to perform molecular *** experimental validation using renal tubular epithelial cells(TCMK-1),we used the Cell Counting Kit-8 assay to detect the effect of NRDP on cell viability,with glucose solution used to mimic a hyperglycemic *** cytometry was used to detect the cell cycle progression and *** blot was used to detect the protein expression of STAT3,p-STAT3,BAX,BCL-2,Caspase9,and *** A total of 10 active ingredients and 85 targets with 111 disease-related signaling pathways were obtained for *** analysis of KEGG pathways was performed to determ