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Functional Characterization of a Type 4 Metallothionein Gene(CsMT4) in Cucumber

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Horticultural Plant Journal 2019年第3期5卷 120-128页

作者： Ling Duan Jingwen Yu Li Xu Ping Tian Xin Hu Xiaomei Song Yu Pan key laboratory of horticulture Science for southern mountainous regions Ministry of Education College of Horticulture and Landscape Architecture Southwest University

The excess amounts of heavy metals are toxic to various physiological processes in *** metallothioneins(MTs)are low molecular weight,cysteine-rich metal-binding proteins that play important roles in the detoxification of heavy metal *** this study,we characterized Cs MT4,a plant type 4 MT gene identified in a complementary DNA(cDNA)library prepared from young cucumber(Cucumis sativus)*** MT4encodes a 90 amino acid protein with a predicted molecular mass of 9.028 ***4 contains 17 cysteine residues in three highly conserved cysteine-rich *** contrast to the structures of other MTs,the highly conserved amino acid pattern CxCxxxCxCxxCxC is present in the middle of ***,CsMT4 was markedly induced in various tissues by various concentrations of Cd^(2+)and Zn^(2+)(>1.0 mmol·L^(-1)).Similar to AtMT4b function,heterologous expression of Cs MT4 in *** could also improved its tolerance to Cd^(2+)and led to increased uptake of Cd^(2+),and the rate of Cd^(2+)uptake was the highest in cells expressing a phytochelatin-like *** findings demonstrate that CsMT4 might be obviously induced by the metal stress in cucumber,and improves tolerance to Cd ions but not Zn ions when heterologously expressed in ***,and suggest that the composition and arrangement of N-terminal Cys-residues in MT4 are associated with their binding capacity and preference for different metal ions.

关键词： Cucumber CsMT4 Metallothionein Metal-binding properties Heterologous expression

SD-RLK28 positively regulates pollen hydration on stigmas as a PCP-Bβ receptor in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2023年第10期65卷 2395-2406页

作者： Li Guo Ziya Huang Xingyu Chen Min Yang Miaomiao Yang Ziwei Liu Xuejie Han Xiangjie Ma Xiaoli Wang Qiguo Gao College of horticulture and Landscape Architecture Chongqing 400716China Ministry of Education Key Laboratory of Horticulture Science for Southern Mountainous RegionsChongqing 400716China

Pollen hydration on dry stigmas is strictly regulated by pollen–stigma interactions in Brassicaceae. Although several related molecular events have been described, the molecular mechanism underlying pollen hydration remains elusive. Multiple B-class pollen coat proteins(PCP-Bs) are involved in pollen hydration. Here, by analyzing the interactions of two PCP-Bs with three Arabidopsis thaliana stigmas strongly expressing S-domain receptor kinase(SD-RLK), we determined that SD-RLK28 directly interacts with PCP-Bβ. We investigated pollen hydration, pollen germination, pollen tube growth, and stigma receptivity in the sd-rlk28 and pcp-bβ mutants. PCP-Bβ acts in the pollen to regulate pollen hydration on stigmas. Loss of SD-RLK28 had no effect on pollen viability, and sd-rlk28 plants had normal life cycles without obvious defects. However,pollen hydration on sd-rlk28 stigmas was impaired and pollen tube growth in sd-rlk28 pistils was delayed. The defect in pollen hydration on sd-rlk28 stigmas was independent of changes in reactive oxygen species levels in stigmas. These results indicate that SD-RLK28 functions in the stigma as a PCP-Bβ receptor to positively regulate pollen hydration on dry stigmas.

关键词： Arabidopsis thaliana PCP-B pollen hydration pollen-stigma interaction reactive oxygen species SD-RLK

Establishment of a tetracycline-off and heat shock-on gene expression system in tobacco

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Journal of Integrative Agriculture 2017年第5期16卷 1112-1119页

作者： ZHOU You LI Jin-hua PAN Yu ZHENG Yu PAN Yang-lu DING Yu-mei SU Cheng-gang ZHANG Xing-guo key laboratory of horticulture Science for southern mountainous regions Ministry of Education/College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715P.R.China

The tetracycline （Tet）-off gene expression regulation system based on the TetR-VP16/Top10 construct has not been widely utilized in plants, for its highly expressed TetR-VP16 activator is toxic to some plants and repeatedly replenishing tetracycline to turn off the constitutively active system is a tedious process. To solve these problems, a Tet-off and heat shock （HS）-on gene expression regulation system was constructed in this study. This system is composed of a chimeric transactivator gene TetR-HSF that is derived from a Tet repressor （TetR） and a HS transcription factor （HSF） controlled by a HS promoter HSP70m, and a Tet operator containing hybrid promoter, Om35S, that drives expression of the β-glucuronidase （GUS） gene. The resultant system yields a GUS expression pattern similar to that of the HSP70m promoter under inducing temperatures and at 35 and 40℃ drives GUS expression to a similar level as the Cauliflower mosaic virus （CaMV） 35S promoter. Further examination revealed that the TetR-HSF and GUS genes were induced by HS, reaching peak expression after 1 and 6 h treatment, respectively, and the HS induction of the expression system could be inhibited by Tet. This system will provide a useful tool for transgenic studies of plants in the laboratory and in the field, including transgene function analysis, agronomic trait improvement, biopharmaceutical protein production and others.

关键词： gene expression system heat shock-on tetracycline-off TetR-HSF transactivator tobacco

A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603

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Journal of Integrative Agriculture 2015年第10期14卷 1949-1957页

作者： PAN Yu CHEN Xu-qing XIE Hua DENG Lei LI Xiang-long ZHANG Xiao-dong HAN Li-xin YANG Feng-ping XUE Jing ZHANG Li-quan Beijing Agro-Biotechnology Research Center Beijing Academy of Agricultural and Forestry Science key laboratory of horticulture Science for southern mountainous regions Ministry of Education/College of Horticulture and Landscape Architecture Southwest University Bioengineering College Chongqing University

The bsd-pg（bundle sheath defective pale green） mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing（SLAF-Seq） technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2：1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats（SSRs） markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.

关键词： maize bsd-pg SLAF SSR assossiation analysis fine mapping

Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System

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Horticultural Plant Journal 2019年第4期5卷 164-169页

作者： Cunfa Ma Mengci Liu Qinfei Li Jun Si Xuesong Ren Hongyuan Song key laboratory of horticulture Science for southern Mountains regions Ministry of EducationCollege of Horticulture and Landscape ArchitectureSouthwest University

Genome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here,we report that the genome of cabbage(Brassica oleracea var. capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacked with multiple single-guide RNA-expressing cassettes. When the phytoene desaturase Bo PDS gene was used as the target gene, an albino-phenotype transgenic shoot in T0 Basta-resistant lines was observed, and 37.5% of the transgenic cabbage shoots carried Bo PDS gene mutations as a result of nucleotide deletions at the expected position. Moreover, mutations were detected in sites with the same target sequence in gene Bol016089 which is paralogous to the Bo PDS gene. Our results show that the CRISPR/Cas9 system is a powerful tool for cabbage variety improvement by genome editing.

关键词： Cabbage CRISPR/Cas9 BoPDS Genome editing Mutation

Comparative Analysis of Three Types of Peaches:Identification of the key Individual Characteristic Flavor Compounds by Integrating Consumers' Acceptability with Flavor Quality

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Horticultural Plant Journal 2017年第1期3卷 1-12页

作者： XI Wanpeng ZHENG Qiao LU Juanfang QUAN Junping College of horticulture and Landscape Architecture Southwest University Agricultural College Shihezi University key laboratory of horticulture Science for southern mountainous regions Ministry of Education Chongqing Nanshan Botanical Garden

In order to recognize the key flavor compounds influencing the consumers' choice for honey peach, nectarine, and flat peach and provide important information for orientation breeding, sugars, organic acids, and aroma volatiles in 3 types of peaches were examined by high performance liquid chromatography(HPLC) and gas chromatography–mass spectrometry(GC–MS) and their key individual characteristic flavor compounds were identified by multivariate analysis integrated with consumers' evaluation. No significant differences in sugar and organic acid were observed between 3 types of peaches. In hierarchical clustering analysis(HCA) and principal component analysis(PCA)models, 3 types of peaches could not be differentiated by sugars or organic acids, but could be identified completely by aroma volatiles or by flavor compounds. A partial least squares regression(PLSR) model revealed that the key individual characteristic volatiles in nectarine, honey peach, and flat peach are C9 compounds and terpenic compounds, norisoprenoids and C6 compounds, and benzaldehyde, γ-decalactone, andδ-dodecalactone, respectively. These results suggest that sugars and organic acids form the background flavor of peach fruit and lactones shape the typical peach aroma. However, the individual aroma volatiles play the decisive role in unique flavor determination of different types of peaches.

关键词： peach sugar organic acid aroma volatile flavor consumer acceptability

Genome assembly of wild loquat (Eriobotrya japonica) and resequencing provide new insights into the genomic evolution and fruit domestication in loquat

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horticulture Research 2023年第2期10卷 147-159页

作者： Danlong Jing Xinya Liu Qiao He Jiangbo Dang Ruoqian Hu Yan Xia Di Wu Shuming Wang Yin Zhang Qingqing Xia Chi Zhang Yuanhui Yu Qigao Guo Guolu Liang key laboratory of horticulture Science for southern Mountains regions of Ministry of Education College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China Academy of Agricultural Sciences of Southwest University State Cultivation Base of Crop Stress Biology for Southern Mountainous LandChongqing 400715China

Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved ***,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding *** of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its *** resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic *** with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic ***,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication ***-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development *** those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit *** high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.

关键词： process insight breeding

Epigenetics in the modern era of crop improvements

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Science China Life Sciences 2025年

作者： Yan Xue Xiaofeng Cao Xiangsong Chen Xian Deng Xingwang Deng Yong Ding Aiwu Dong Chengguo Duan Xiaofeng Fang Lei Gong Zhizhong Gong Xiaofeng Gu Chongsheng He Hang He Shengbo He Xinjian He Yan He Yuehui He Guifang Jia Danhua Jiang Jianjun Jiang Jinsheng Lai Zhaobo Lang Chenlong Li Qing Li Xingwang Li Bao Liu Bing Liu Xiao Luo Yijun Qi Weiqiang Qian Guodong Ren Qingxin Song Xianwei Song Zhixi Tian Jiawei Wang Yuan Wang Liang Wu Zhe Wu Rui Xia Jun Xiao Lin Xu Zhengyi Xu Wenhao Yan Hongchun Yang Jixian Zhai Yijing Zhang Yusheng Zhao Xuehua Zhong Daoxiu Zhou Ming Zhou Yue Zhou Bo Zhu Jiankang Zhu Qikun Liu College of Life Sciences Institute of Life Science and Green Development Hebei University Biotechnology Research Institute Chinese Academy of Agricultural Sciences College of Biology Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan Engineering and Technology Research Center of Hybrid Rapeseed Hunan University Institute of Advanced Agricultural Sciences School of Advanced Agricultural Sciences Peking University State key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University National Institute of Biological Sciences key laboratory of Seed Innovation Institute of Genetics and Developmental Biology Chinese Academy of Sciences School of Advanced Agricultural Sciences Peking-Tsinghua Center for Life Sciences Peking University Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education College of Chemistry and Molecular Engineering Peking University key laboratory of Seed Innovation State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State key laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang State key laboratory of Crop Stress Adaptation and Improvement School of Life Sciences Henan University State key laboratory of Maize Bio-breeding National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University Frontiers Science Center for Molecular Design Breeding China Agricultural University Center for Crop Functional Genomics and Molecular Breeding China Agricultural University Sanya Institute of China Agricultural University Hainan Yazhou Bay Seed laboratory Institute of Advan

Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades,our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.

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Phenolic and flavonoid contents of mandarin (Citrus reticulata Blanco) fruit tissues and their antioxidant capacity as evaluated by DPPH and ABTS methods

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Journal of Integrative Agriculture 2018年第1期17卷 256-263页

作者： ZHANG Hua YANG Yi-fei ZHOU Zhi-qin College of horticulture and Landscape Architecture Southwest University College of Life Science & Engineering Chongqing Three Gorges University key laboratory of horticulture for southern mountainous regions Ministry of Education laboratory of Quality & Safety Risk Assessment for Citrus Products of Ministry of Agriculture Citrus Research InstituteSouthwest University

The total phenolic and flavonoid contents in the fruit tissues （peels, pulp residues, seeds, and juices） of 19 citrus genotypes belonged to Citrus reticulata Blanco were evaluated and their antioxidant capacity was tested by 2,2-diphenyl-l-picrylhydra- zyl radicals （DPPH） method and 2,2＇-azino-bis（3-ethylbenzthiozoline-6）-sulphonic acid （ABTS） method. The total phenolic and flavonoid contents, and their antioxidant capacity varied in different citrus fruit tissues. Generally, the peel had both the highest average of total phenolics （27.18 mg gallic acid equivalent （GAE） g^-1 DW） and total flavonoids （38.97 mg rutin equivalent （RE） g^-1 DW）. The highest antioxidant capacity was also the average of DPPH value （21.92 mg vitamin C equiv- alent antioxidant capacity （VCEAC） g^-1 DW） and average of ABTS value （78.70 mg VCEAC g-1 DW） in peel. The correlation coefficient between the total phenolics and their antioxidant capacity of different citrus fruits tissues ranged from 0.079 to 0.792, and from -0.150 to 0.664 for the total flavonoids. The antioxidant capacity of fruit tissues were correlated with the total phenoilc content and flavonoid content except in case of the peel. In addition, the total phenolic content and antioxidant capacity varied in different citrus genotypes. Manju and Karamandarin were better genotypes with higher antioxidation and the phenolic content, however Shagan was the poorest genotype with lower antioxidation and the phenolic content.

关键词： Citrus L. fruit tissues phenolics/flavonoids antioxidant capacity