Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System

作     者：Cunfa Ma Mengci Liu Qinfei Li Jun Si Xuesong Ren Hongyuan Song Cunfa Ma;Mengci Liu;Qinfei Li;Jun Si;Xuesong Ren;Hongyuan Song

作者机构：Key Laboratory of Horticulture Science for Southern Mountains RegionsMinistry of EducationCollege of Horticulture and Landscape ArchitectureSouthwest University 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2019年第5卷第4期

页      面：164-169页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by the National Key Research and Development Program of China (2017YFD0101804) the Scientific and Technological Innovation Projects for the Social Undertakings and the People’s Livelihood in Chongqing (cstc2015shmsztzx80005 cstc2015shms-ztzx80007 cstc2015shms-ztzx80009) 

主　　题：Cabbage CRISPR/Cas9 BoPDS Genome editing Mutation 

摘      要：Genome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here,we report that the genome of cabbage(Brassica oleracea var. capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacked with multiple single-guide RNA-expressing cassettes. When the phytoene desaturase Bo PDS gene was used as the target gene, an albino-phenotype transgenic shoot in T0 Basta-resistant lines was observed, and 37.5% of the transgenic cabbage shoots carried Bo PDS gene mutations as a result of nucleotide deletions at the expected position. Moreover, mutations were detected in sites with the same target sequence in gene Bol016089 which is paralogous to the Bo PDS gene. Our results show that the CRISPR/Cas9 system is a powerful tool for cabbage variety improvement by genome editing.