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Journal of Pharmaceutical Analysis 2024年第3期14卷 371-388页

作者： Haonan Ruan Jing Zhang Yunyun Wang Ying Huang Jiashuo Wu Chunjiao He Tongwei Ke Jiaoyang Luo Meihua Yang key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100193China

Zearalenone(ZEN)is a mycotoxin that extensively contaminates food and feed,posing a significant threat to public ***,the mechanisms behind ZEN-induced intestinal immunotoxicity remain *** this study,Sprague-Dawley(SD)rats were exposed to ZEN at a dosage of 5 mg/kg/day *** a duration of 14 *** results demonstrated that ZEN exposure led to notable pathological alterations and immunosuppression within the ***,ZEN exposure caused a significant reduction in the levels of apolipoprotein E(ApoE)and liver X receptor(LXR)(P<0.05).Conversely,it upregulated the levels of myeloid-derived suppressor cells(MDSCs)markers(P<0.05)and decreased the presence of 27-hydroxycholesterol(27-HC)in the intestine(P<0.05).It was observed that ApoE or LXR agonists were able to mitigate the immunosuppressive effects induced by ***,a bioinformatics analysis highlighted that the downregulation of ApoE might elevate the susceptibility to colorectal,breast,and lung *** findings underscore the crucial role of the 27-HC/LXR/ApoE axis disruption in ZEN-induced MDSCs proliferation and subsequent inhibition of T lymphocyte activation within the rat ***,ApoE may emerge as a pivotal target linking ZEN exposure to cancer development.

关键词： Zearalenone Intestinal immunosuppression Apolipoprotein E Bioinformatics analysis Cancer

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional chinese medicines

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chinese herbal medicines 2024年第2期16卷 180-189页

作者： Kailin Yang Yaping Zheng Kangmeng Sun Xinyan Wu Zheng Zhang Chunnian He Peigen Xiao Institute of Medicinal Plant Development Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of EducationBeijing 100193China

Rhizosphere microorganisms,as one of the most important components of the soil microbiota and plant holobiont,play a key role in the medicinal plant-soil ecosystem,which are closely related to the growth,adaptability,nutrient absorption,stress tolerance and pathogen resistance of host *** recent years,with the wide application of molecular biology and omics technologies,the outcomes of rhizosphere microorganisms on the health,biomass production and secondary metabolite biosynthesis of medicinal plants have received extensive ***,whether or to what extent rhizosphere microorganisms can contribute to the construction of the quality evaluation system of chinese medicinal materials is still *** on the significant role of rhizosphere microbes in the survival and quality formation of medicinal plants,this paper proposed a new concept of rhizosphere microbial markers(micro-markers),expounded the relevant research methods and ideas of applying the new concept,highlighted the importance of micro-markers in the quality evaluation and control system of traditional chinese medicines(TCMs),and introduced the potential value in soil environmental assessment,plant pest control and quality assessment of *** provides reference for developing ecological planting of TCMs and ensuring the production of high quality TCMs by regulating rhizosphere microbial communities.

关键词： physical examination indicator quality evaluation quality formation of medicinal plants rhizosphere microorganisms rhizosphere microbial markers

Perspective and challenges of mycorrhizal symbiosis in orchid medicinal plants

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chinese herbal medicines 2024年第2期16卷 172-179页

作者： Chunyan Leng Mengyan Hou Yongmei Xing Juan Chen key laboratory of bioactive substances and Resource utilization of chinese herbal medicine Ministry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China

The family Orchidaceae is of the most diverse taxon in the plant kingdom,and most of its members are highly valuable herbal *** have a unique mycorrhizal symbiotic relationship with specific fungi for carbohydrate and nutrient supplies in their whole *** large-scale cultivation of the medicinal plant Gastodia elata is a successful example of using mycorrhizal symbiotic *** this review,we adopted *** and Dendrobium officinale as examples to describe the characteristics of orchid mycorrhiza and mycorrhizal benefits for host plants’growth and health(*** and abiotic stress and secondary metabolite accumulation).The challenges in applying mycorrhizal technology to the cultivation of orchid medicinal plants in the future were also *** review aims to serve as a theoretical guide for the cultivation of mycorrhizal technology in medicinal orchid plants.

关键词： Dendrobium officinale Kimura&Migo ecological cultivation Gastodia elata Blume mycorrhiza orchid medicinal plants symbiosis mechanisms

Self-adjuvant Astragalus polysaccharide-based nanovaccines for enhanced tumor immunotherapy:a novel delivery system candidate for tumor vaccines

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Science China(Life Sciences) 2024年第4期67卷 680-697页

作者： Nan Li Yun Zhang Miaomiao Han Tian Liu Jinjia Wu Yingxia Xiong Yikai Fan Fan Ye Bing Jin Yinghua Zhang Guibo Sun Xiaobo Sun Zhengqi Dong State key laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Chinese Academy of Medical Sciences&Peking Union Medical College(CAMS)Institute of Medicinal Plant Development(IMPLAD)Beijing 100193China key laboratory of bioactive substances and resources utilization of chinese herbal medicine from ministry of education Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine(Natural Medicine)and Translational MedicineKey Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription from Chinese Academy of Medical SciencesCAMSIMPLADBeijing 100193China Department of Pharmaceutics School of Pharmaceutical SciencesHeilongjiang University of Chinese MedicineHarbin 150036China Jilin Academy of chinese medicine Sciences Changchun 130012China Joint Research Center for chinese Medicinal Herbs IMPLADABRC&ACCLBeijing 100193China

The study of tumor nanovaccines(NVs)has gained interest because they specifically recognize and eliminate tumor ***,the poor recognition and internalization by dendritic cells(DCs)and insufficient immunogenicity restricted the vaccine ***,we extracted two molecular-weight Astragalus polysaccharides(APS,12.19 k D;APSHMw,135.67 k D)from Radix Astragali and made them self-assemble with OVA257–264directly forming OVA/APS integrated nanocomplexes through the microfluidic *** nanocomplexes were wrapped with a sheddable calcium phosphate layer to improve *** in the formed nanocomplexes served as drug carriers and immune adjuvants for potent tumor *** optimal APS-NVs were approximately 160 nm with uniform size distribution and could remain stable in physiological saline *** FITC-OVA in APS-NVs could be effectively taken up by DCs,and APS-NVs could stimulate the maturation of DCs,improving the antigen cross-presentation efficiency in *** possible mechanism was that APS can induce DC activation via multiple receptors such as dectin-1 and Toll-like receptors 2 and *** accumulation of APS-NVs both in draining and distal lymph nodes were observed following *** APS-NVs could easily access the lymph ***,APS-NVs could markedly promote antigen delivery efficiency to DCs and activate cytotoxic T *** addition,APS-NVs achieve a better antitumor effect in established B16-OVA melanoma tumors compared with the OVA+Alum treatment *** antitumor mechanism correlated with the increase in cytotoxic T cells in the tumor ***,the poor tumor inhibitory effect of APS-NVs on the nude mouse model of melanoma also confirmed the participation of antitumor adaptive immune response induced by ***,this study developed a promising APS-based tumor NV that is an efficient tumor immunotherapy without systemic side effects.

关键词： Astragalus polysaccharides nanovaccines OVA immunotherapy self-adjuvant

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Isolation of exogenous fungi from Notoginseng Radix et Rhizoma and preliminary analysis of toxigenic fungi

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Journal of Future Foods 2024年第2期4卷 179-182页

作者： Lei Zhou Xiangsheng Zhao Xiaomin Liu Hefang Wan Chun Sui Xuyu Chen key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of EducationNational Engineering Laboratory for Breeding of Endangered Medicinal MaterialsInstitute of Medicinal Plant Development(IMPLAD)Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Hainan Provincial key laboratory of resources Conservation and Development of Southern medicine Hainan Branch of the Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeHaikou 570311China

Notoginseng Radix et Rhizoma(Sanqi in chinese)is a precious traditional chinese herbal *** has the effect of dispersing blood stasis and stopping bleeding,reducing swelling and fixing ***,it tends to contaminate with harmful fungi during storage,which may make it much less *** order to understand the fungal contamination of Notoginseng Radix et Rhizoma and master its composition of the exogenous *** surface fungi of Notoginseng Radix et Rhizoma samples collected from six chinese provinces and districts were investigated by using dilution plate *** of aflatoxins by UPLC-MS/*** results showed that Penicillium citrinum was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.1 to *** flavus,which produces aflatoxin,was dominantly isolated from Notoginseng Radix et Rhizoma samples from No.5 and *** addition,kinds of mycotoxin were assayed which were produced by three of those identified *** three fungi strains produced aflatoxin B1(AFB1)and one strain HBSQ1-5 additionally produced other three kinds of mycotoxin,AFB2,AFG1 and *** is the results implied that it will be very important to take serious cautions when using Notoginseng Radix et *** well as,understanding the composition of the exogenous fungi of Notoginseng Radix et Rhizoma and the strains of toxin-producing fungi,which can play an important role in guiding the storage of Notoginseng Radix et Rhizoma.

关键词： Notoginseng Radix et Rhizoma Exogenous fungal Amplicon sequencing Mycotoxin

Identifying Qingkailing(清开灵)ingredients-dependent mesenchymal-epithelial transition factor-axiation“π”structuring module with angiogenesis and neurogenesis effects

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Journal of Traditional chinese medicine 2024年第1期44卷 35-43页

作者： CHENG Kunming YUAN Jianan LIU Jun ZHANG Shengpeng XU Qixiang XIE Yong ZHAO Jingfeng ZHANG Xiaoxu TANG Xudong ZHENG Yongqiu WANG Zhong Provincial Engineering laboratory for Screening and Re-evaluation of Active Compounds of herbal medicines in Southern Anhui Teaching and Research Section of Traditional Chinese MedicineSchool of PharmacyWannan Medical CollegeWuhu 241000China Institute of Basic Research in Clinical medicine China Academy of Chinese Medical SciencesBeijing 100700China key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of EducationDepartment of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Department of Gastroenterology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE:To explore the functional role of the drugdependent mesenchymal-epithelial transition(Met)-axiation"π"structural module of neurogenesis after processing by three components of Qingkailing injection in neurogenesis and angiogenesis in cerebral ***:We used a Glutathione S-transferase(GST)-pull down assay,isothermal titration calorimetry assay,and other related methods to identify the relationships among Met,inositol polyphosphate phosphatase like 1(Inppl1),and death associated protein kinase 3(Dapk3)in this allosteric *** biological effects of the modules of neurons generation composed of Met,Inppl1,and Dapk3 were measured through Western blot,apoptosis analysis,and double immunofluorescence ***:The GST-pull down assay revealed that proline-serine-threonine rich domain of Met binds to the Src homology domain of Inppl1 to form a protein-protein complex;Dapk3 with a C-terminal domain interacts weakly with the protein kinase C domain of Met in the intracellular ***,we obtained a“π”structuring module considered a neural regeneration *** biological effects of angiogenesis and neurogenesis modules composed of Met,Inppl1,and Dapk3 were also ***:The study suggested that understanding the functional modules that contribute to pharmaceutics might provide novel signatures that can be used as endpoints to define disease processes under stroke or cerebral ischemia conditions.

关键词： brain ischemia stroke neurogenesis angiogenesis modular pharmacology Met-axiation allosteric module

Microbial interactions within chinese traditional medicinal plants

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chinese herbal medicines 2024年第2期16卷 169-171页

作者： Juan Chen Luping Qin key laboratory of bioactive substances and Resource utilization of chinese herbal medicine Ministry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China School of Pharmaceutical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053China

Plants harbor diverse microbes(including bacteria,fungi,archaea,protists,and viruses)both inside and outside their tissues,so called the plant-associated *** of research have demonstrated the importance of plant microbiome in promoting the productivity and health of the plant in natural environment because of their essential functions in improving plant nutrition and plant resistance to biotic and abiotic stresses(Trivedi,Leach,Tringe,Sa,&Singh,2020).Thus,a plant can be regarded as a holobiont comprising the host plant and the associated microbiota(Hassani,Durán,&Hacquard,2018).Within the plant microbiota,mutualistic fungal and bacterial symbionts(*** fungi and Rhizobia)are striking examples of microorganisms playing crucial roles in nutrient acquisition(Martin&van der Heijden,2024)and non-symbitic plant growth-promoting rhizobacteria or fungi(PGPR or PGPF)also have drawn interest due to their ability to improve soil properties and confer stress tolerance in plants(Upadhyay et al.,2023).

关键词： mutual striking outside

Strategies on biosynthesis and production of bioactive compounds in medicinal plants

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chinese herbal medicines 2024年第1期16卷 13-26页

作者： Miaoxian Guo Haizhou Lv Hongyu Chen Shuting Dong Jianhong Zhang Wanjing Liu Liu He Yimian Ma Hua Yu Shilin Chen Hongmei Luo key Lab of chinese medicine resources Conservation State Administration of Traditional Chinese Medicine of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China Engineering Research Center of chinese medicine Resource Ministry of EducationBeijing 100193China key laboratory of Hangzhou City for Ecosystem Protection and Restoration College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China Chengdu University of Traditional chinese medicine Chengdu 611137China

Medicinal plants are a valuable source of essential medicines and herbal products for healthcare and disease *** with chemical synthesis and extraction,the biosynthesis of natural products is a very promising alternative for the successful conservation of medicinal plants,and its rapid development will greatly facilitate the conservation and sustainable utilization of medicinal ***,we summarize the advances in strategies and methods concerning the biosynthesis and production of natural products of medicinal *** strategies and methods mainly include genetic engineering,plant cell culture engineering,metabolic engineering,and synthetic biology based on multiple“OMICS”technologies,with paradigms for the biosynthesis of terpenoids and *** also highlight the biosynthetic approaches and discuss progress in the production of some valuable natural products,exemplifying compounds such as vindoline(alkaloid),artemisinin and paclitaxel(terpenoids),to illustrate the power of biotechnology in medicinal plants.

关键词： biosynthetic strategies biotechnology natural products medicinal plants synthetic biology

Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim

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Synthetic and Systems Biotechnology 2022年第4期7卷 1095-1107页

作者： Yu Yao Jiajun Gu Yanjiao Luo Yuanyue Wang Yongzhen Pang Guoan Shen Baolin Guo key laboratory of bioactive substances and resources utilization of chinese herbal medicines Ministry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing100193China Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing100193China

Epimedium pubescens *** a well-known traditional chinese medicinal herb with flavonol glycosides as the major pharmaceutically active ***-glycosyltransferases(UGTs)are a group of enzymes responsible for the glycosylation of flavonoid *** this study,a genome-wide analysis was performed to identify UGT family genes in *** a result,a total of 339 putative UGT genes were identified,which represents the largest UGT gene family known thus far,implying a significant expansion of the UGT gene family in *** EpUGTs were unevenly distributed across six chromosomes,and they were classified into 17 major *** expression profiles showed that UGT genes were differentially expressed in roots,leaves,flowers,shoots and *** particular,several EpUGTs were highly induced by high light intensity,which was consistent with the accumulation level of bioactive flavonoids in *** UGT79 genes that were preferentially expressed in roots or leaves were successfully expressed in ***,and only the recombinant EpGT60 protein was found to be active toward 8-prenylkaempferol and icaritin to produce the key bioactive compounds baohuoside II and baohuoside *** optimal temperature,pH,k_(m) and V_(max) were determined for the recombinant EpGT60 *** addition,expression of recombinant EpGT60 in *** cell culture led to successful production of baohuoside II when fed *** study provides a foundation for further functional characterization of UGT genes in *** and provides key candidate genes for bioengineering bioactive flavonoids in ***.

关键词： Epimedium pubescens Maxim Flavonoids UDP-glycosyltransferase Phylogenetic analysis EpGT60

Mycorrhizal fungal community composition in seven orchid species inhabiting Song Mountain, Beijing, China

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Science China(Life Sciences) 2019年第6期62卷 838-847页

作者： Yanhong Chen Yue Gao Linli Song Zeyu Zhao Shunxing Guo Xiaoke Xing key laboratory of bioactive substances and resources utilization of chinese herbal medicine Ministry of Education Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College

Mycorrhizal fungi play an important role in the germination and growth of orchids essentially influencing their survival,abundance, and spatial distribution. In this study, we investigated the composition of the mycorrhizal fungal community in seven terrestrial orchid species inhabiting Song Mountain, Beijing, China, using Illumina MiSeq high-throughput sequencing. The mycorrhizal communities in the seven orchids were mainly composed of members of the Ceratobasidiaceae, Sebacinales, and Tulasnellaceae, while a number of ectomycorrhizal fungi belonging to the Russulaceae, Tricholomataceae, Thelephoraceae, and Cortinariaceae were occasionally observed. However, the dominant fungal associates and mycorrhizal community differed significantly among the orchid species as well as subhabitats. These findings confirm the previous observation that sympatric orchid species show different preferences for mycorrhizal fungi, which may drive niche partitioning and contribute to their cooccurrence.

关键词： orchid mycorrhiza fungal community terrestrial orchids fungal diversity