Identifying Qingkailing(清开灵)ingredients-dependent mesenchymal-epithelial transition factor-axiation“π”structuring module with angiogenesis and neurogenesis effects

作     者：CHENG Kunming YUAN Jianan LIU Jun ZHANG Shengpeng XU Qixiang XIE Yong ZHAO Jingfeng ZHANG Xiaoxu TANG Xudong ZHENG Yongqiu WANG Zhong CHENG Kunming;YUAN Jianan;LIU Jun;ZHANG Shengpeng;XU Qixiang;XIE Yong;ZHAO Jingfeng;ZHANG Xiaoxu;TANG Xudong;ZHENG Yongqiu;WANG Zhong

作者机构：Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern AnhuiTeaching and Research Section of Traditional Chinese MedicineSchool of PharmacyWannan Medical CollegeWuhu 241000China Institute of Basic Research in Clinical MedicineChina Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal MedicineMinistry of EducationDepartment of Medicinal Plant DevelopmentChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Department of GastroenterologyXiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2024年第44卷第1期

页      面：35-43页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：National Major Scientific and Technological Special Project for“Significant New Drugs Development”:Clinical Value Prescription Discovery and Evaluation Technology Based on Modular Pharmacology(No.2017ZX09301059) the Joint Innovation Project of China Academy of Chinese Medical Sciences:Study on the Mechanism of Action of Neuronal Regenerative Function Module Activated by Qingkailing Components(No.ZZ11-026) the University Collaborative Innovation Project of Anhui:Creation of a Combined Animal Model of Coronary Heart Disease Based on the Theory of Xin'an Medicine(No.GXXT-2020-024) the University Natural Science Research Project of Anhui Province:Molecular Mechanism of the Anti-hepatic Fibrosis Effect of Wickerwork Amide Based on the Regulation of Intercellular Communication by PTRF(No.2023AH051752) 

主　　题：brain ischemia stroke neurogenesis angiogenesis modular pharmacology Met-axiation allosteric module 

摘      要：OBJECTIVE:To explore the functional role of the drugdependent mesenchymal-epithelial transition(Met)-axiationπstructural module of neurogenesis after processing by three components of Qingkailing injection in neurogenesis and angiogenesis in cerebral ***:We used a Glutathione S-transferase(GST)-pull down assay,isothermal titration calorimetry assay,and other related methods to identify the relationships among Met,inositol polyphosphate phosphatase like 1(Inppl1),and death associated protein kinase 3(Dapk3)in this allosteric *** biological effects of the modules of neurons generation composed of Met,Inppl1,and Dapk3 were measured through Western blot,apoptosis analysis,and double immunofluorescence ***:The GST-pull down assay revealed that proline-serine-threonine rich domain of Met binds to the Src homology domain of Inppl1 to form a protein-protein complex;Dapk3 with a C-terminal domain interacts weakly with the protein kinase C domain of Met in the intracellular ***,we obtained a“πstructuring module considered a neural regeneration *** biological effects of angiogenesis and neurogenesis modules composed of Met,Inppl1,and Dapk3 were also ***:The study suggested that understanding the functional modules that contribute to pharmaceutics might provide novel signatures that can be used as endpoints to define disease processes under stroke or cerebral ischemia conditions.