Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim
作者机构：Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal MedicinesMinistry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing100193China Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2022年第7卷第4期
页 面：1095-1107页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：This work was supported by grants from the CAMS Innovation Fund for Medical Sciences(CIFMS)(2021-I2M-1-031 and 2017-I2M-3-013) the National Natural Science Foundation of China(31570306 and 81473302)
主 题：Epimedium pubescens Maxim Flavonoids UDP-glycosyltransferase Phylogenetic analysis EpGT60
摘 要：Epimedium pubescens *** a well-known traditional Chinese medicinal herb with flavonol glycosides as the major pharmaceutically active ***-glycosyltransferases(UGTs)are a group of enzymes responsible for the glycosylation of flavonoid *** this study,a genome-wide analysis was performed to identify UGT family genes in *** a result,a total of 339 putative UGT genes were identified,which represents the largest UGT gene family known thus far,implying a significant expansion of the UGT gene family in *** EpUGTs were unevenly distributed across six chromosomes,and they were classified into 17 major *** expression profiles showed that UGT genes were differentially expressed in roots,leaves,flowers,shoots and *** particular,several EpUGTs were highly induced by high light intensity,which was consistent with the accumulation level of bioactive flavonoids in *** UGT79 genes that were preferentially expressed in roots or leaves were successfully expressed in ***,and only the recombinant EpGT60 protein was found to be active toward 8-prenylkaempferol and icaritin to produce the key bioactive compounds baohuoside II and baohuoside *** optimal temperature,pH,k_(m) and V_(max) were determined for the recombinant EpGT60 *** addition,expression of recombinant EpGT60 in *** cell culture led to successful production of baohuoside II when fed *** study provides a foundation for further functional characterization of UGT genes in *** and provides key candidate genes for bioengineering bioactive flavonoids in ***.
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