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Phosphorylation of PB2 at serine 181 restricts viral replication and virulence of the highly pathogenic H5N1 avian influenza virus in mice

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Virologica Sinica 2024年第1期39卷 97-112页

作者： Jiao Hu Zixiong Zeng Xia Chen Manyu Zhang Zenglei Hu Min Gu Xiaoquan Wang Ruyi Gao Shunlin Hu Yu Chen Xiaowen Liu Daxin Peng Xiufan Liu Animal Infectious Disease laboratory School of Veterinary MedicineYangzhou UniversityYangzhou225009China Joint International Research laboratory of agriculture and agri-Product safety The Ministry of Education of ChinaYangzhou University225009China Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou UniversityYangzhou225009China key laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin)for agri-food safety and Quality Ministry of Agriculture of China(26116120)Yangzhou UniversityYangzhou225009China

Influenza A virus(IAV)continues to pose a pandemic threat to public health,resulting a high mortality rate annually and during pandemic years.Posttranslational modification of viral protein plays a substantial role in regulating IAV infection.Here,based on immunoprecipitation(IP)-based mass spectrometry(MS)and purified virus-coupled MS,a total of 89 phosphorylation sites distributed among 10 encoded viral proteins of IAV were identified,including 60 novel phosphorylation sites.Additionally,for the first time,we provide evidence that PB2 can also be acetylated at site K187.Notably,the PB2 S181 phosphorylation site was consistently identified in both IP-based MS and purified virus-based MS.Both S181 and K187 are exposed on the surface of the PB2 protein and are highly conserved in various IAV strains,suggesting their fundamental importance in the IAV life cycle.Bioinformatic analysis results demonstrated that S181E/A and K187Q/R mimic mutations do not significantly alter the PB2 protein structure.While continuous phosphorylation mimicked by the PB2 S181E mutation substantially decreases viral fitness in mice,PB2 K187Q mimetic acetylation slightly enhances viral virulence in mice.Mechanistically,PB2 S181E substantially impairs viral polymerase activity and viral replication,remarkably dampens protein stability and nuclear accumulation of PB2,and significantly weakens IAV-induced inflammatory responses.Therefore,our study further enriches the database of phosphorylation and acetylation sites of influenza viral proteins,laying a foundation for subsequent mechanistic studies.Meanwhile,the unraveled antiviral effect of PB2 S181E mimetic phosphorylation may provide a new target for the subsequent study of antiviral drugs.

关键词： H5N1 influenza virus PB2 Phosphorylation Acetylation Viral fitness Mice

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Metabolic profiles and morphological characteristics of leaf tips among different sweet potato(Ipomoea batatas Lam.)varieties

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Journal of Integrative agriculture 2024年第2期23卷 494-510页

作者： Wenqing Tan Xinbo Guo Zhangying Wang Rong Zhang Chaochen Tang Bingzhi Jiang Ruixue Jia Yuanyuan Deng Shaohai Yang Jingyi Chen Crops Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Crop Genetic Improvement of Guangdong ProvinceGuangzhou 510640China School of food Science and Engineering South China University of TechnologyGuangzhou 510640China Sericultural&agri-food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional FoodsMinistry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China

Sweet potato leaf tips have high nutritional value,and exploring the differences in the metabolic profiles of leaf tips among different sweet potato varieties can provide information to improve their qualities.In this study,a UPLC-Q-Exactive Orbitrap/MS-based untargeted metabolomics method was used to evaluate the metabolites in leaf tips of 32 sweet potato varieties.Three varieties with distinct overall metabolic profiles(A01,A02,and A03),two varieties with distinct profiles of phenolic acids(A20 and A18),and three varieties with distinct profiles of flavonoids(A05,A12,and A16)were identified.In addition,a total of 163 and 29 differentially expressed metabolites correlated with the color and leaf shape of sweet potato leaf tips,respectively,were identified through morphological characterization.Group comparison analysis of the phenotypic traits and a metabolite-phenotypic trait correlation analysis indicated that the color differences of sweet potato leaf tips were markedly associated with flavonoids.Also,the level of polyphenols was correlated with the leaf shape of sweet potato leaf tips,with lobed leaf types having higher levels of polyphenols than the entire leaf types.The findings on the metabolic profiles and differentially expressed metabolites associated with the morphology of sweet potato leaf tips can provide useful information for breeding sweet potato varieties with higher nutritional value.

关键词： sweet potato leaf tips phenotypic traits metabolic profile differentially expressed metabolites polyphenols

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Antibacterial mechanism of kojic acid and tea polyphenols against Escherichia coli O157:H7 through transcriptomic analysis

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food Science and Human Wellness 2024年第2期13卷 736-747页

作者： Yilin Lin Ruifei Wang Xiaoqing Li Keren Agyekumwaa Addo Meimei Fang Yehui Zhang Yigang Yu School of food Sciences and Engineering South China University of TechnologyGuangzhou 510640China Sericulture&agri-food Research Institute Guangdong Academy of agricultural Sciences Key Laboratory of Functional FoodsMinistry of Agriculture and Rural AffairsGuangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China Research Center of food safety and Detection College of Food Science and EngineeringSouth China University of TechnologyGuangzhou 510640China

Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity against E.coli O157:H7 than using either alone.This study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene level.The functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against E.coli O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic process.Interestingly,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid cycle.The results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against E.coli O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.

关键词： Kojic acid Tea polyphenols Antibacterial mechanism Escherichia coli O157:H7 RNA-seq

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Effect of purine diet on prognosis of deep brain stimulation for Parkinson's disease

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food Science and Human Wellness 2024年

作者： Bowen Chang Yuge Jiang Chuanxing Feng Bowen Li Jiaming Mei Chaoshi Niu key laboratory of Xin'an Medicine Ministry of Education anhui province key laboratory of R&D of Chinese Medicine anhui University of Chinese Medicine 3. Center for food Nutrition and Functional food Engineering School of food Science and Technology Jiangnan University Department of Neurosurgery The First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China

Uric acid (UA) is a naturally antioxidant that is strongly associated with the development and progression of Parkinson’s disease (PD). The purine diet is an important exogenous pathway that modulates blood UA levels. Deep brain stimulation (DBS) is an important tool for PD treatment. This study aimed to explore the effects of preoperative purine diet on the prognosis of patients with PD after DBS. 64 patients with PD who underwent DBS were included in this study， and their clinical data， blood UA values， and daily purine intake. Patients were followed up for improvement 1 year after surgery. We found that patient higher purine intake was strongly associated with the rate of improvement after DBS and was a protective factor for patient prognosis. Daily purine intake from meat and seafood was significantly higher in the responsive patients than in the less-responsive patients. Mediation analysis showed that UA mediated 78% of the effect of purine intake on motor symptom improvement after DBS. In summary， we observed that purine intake is strongly associated with the rate of improvement in motor symptoms after STN-DBS in patients with PD. This study provides a reference for preoperative diet planning in patients with Parkinson’s disease undergoing DBS.

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Gut microbiota-mediated modulation of lipid metabolism by punicic acid in diet-induced hepatic steatosis

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food Science and Human Wellness 2024年

作者： Liping Chen Yifan Lei Hengqian Lu Yongzhong Wang School of Life Sciences anhui University anhui key laboratory of Modern Biomanufacturing key laboratory of Human Microenvironment and Precision Medicine of anhui Higher Education Institutes anhui University

Pomegranate seed oil (PSO) mainly consisted of punicic acid (PunA)， has gained attention due to rich conjugated linolenic acids and potential application in the improvement of various diseases. The aim of this study was to explore the ability of PunA to modulate lipid metabolism associated with non-alcoholic fatty liver disease (NAFLD) and explore the emerging role of the gut microbiota in modulating hepatic lipid metabolism. In this regard， PunA’s capacity was evaluated through C57BL/6J fed with a choline and L-amino acid defined， high fat diet (CDAHFD). Mice were randomly divided into four groups: NC group， MC group， PSO group and Cur group. Collected results indicated that supplement of PunA improved hepatic lipid profile. PunA administration also ameliorated hepatic steatosis and lipid accumulation. mRNA analysis showed that PunA exerted ameliorative e?ect on hepatic lipid accumulation by downregulating the mRNA expression of Dgat1， Scd-1， Srebp-1c， but increasing expression level of Cpt-1α. Metagenomic analysis discovered that PunA increased species diversity and richness of intestinal microbiota， promoted proliferation of beneficial commensals and restraining the endotoxin-producing microorganisms in cecum of CDAHFD-administrated mice. Furthermore， PunA suppressed lipid accumulation and lipid droplet formation in a cell NAFLD model in vitro. Overall， PunA exhibited anti-lipogenic and lipid-lowering effects， improvement of intestinal flora. In summary， PunA could be a candidate for the exploitation of functional foods or nutraceuticals aiming NAFLD.

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Aging-induced YTHDF aggregates impair mitochondrial function by trapping mitochondrial RNAs and suppressing their expression in the brain

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Protein & Cell 2024年第2期15卷 149-155页

作者： Juan Zhang Dingfeng Li Keqiang He Qiang Liu Zhongwen Xie School of Life Sciences Anhui Agricultural UniversityHefei 230036China State key laboratory of Tea Plant Biology and Utilization School of Tea and Food Sciences and TechnologyAnhui Agricultural UniversityHefei230036China Division of Life Sciences and Medicine Hefei National Research Center for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230026China Department of Anesthesiology The First Affliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China anhui province key laboratory of Biomedical Aging Research University of Science and Technology of ChinaHefei 230026China Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China

DearEditor,Brain aging is associated with a decrease in cognitive function,which is often accompanied by defective changes in mitochondrial morphology and reduction in mitochondrial function(Faitg et al.,2021).The main function of mitochondria is to produce energy in the form of ATP,which is vital to fulfill high energy demand of neurons,mainly for synaptic processes(Lee et al.,2018).Mitochondrial dysfunction acts as a key factor for age-related cognitive decline(Grimm and Eckert,2017).

关键词： function trapping

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Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus

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food Science and Human Wellness 2024年第4期13卷 2161-2173页

作者： Tenggen Hu Yuanshan Yu Jijun Wu Yujuan Xu Gengsheng Xiao Kejing An Erna Li Sentai Liao Yuxiao Zou Sericultural&agri-food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional FoodsMinistry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China Huagongliya(Foshan)Technology Industry Co. Ltd.Foshan 528313China Heyuan Branch Guangdong Laboratory for Lingnan Modern AgricultureHeyuan 517001China Zhongkai University of agriculture and Engineering Guangzhou 510225China

Two oligosaccharide fractions(MLO 2-1 and 2-2)were purified from enzymatic hydrolysate of mulberry leaf polysaccharide.The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide,which could be degraded by human digestive juice;while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota.Hence,MLO 2-1 was selected for the further analysis.The structure of MLO 2-1 was elucidated as follow:α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1.The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota.Meanwhile,MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids,especially acetate and butyrate.The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated.Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus,a commensal bacterium presented a reduced level in T2DM mice.Animal experiments indicated that MLO 2-1 and L.murinus exhibited hypoglycemic activities.These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L.murinus.

关键词： Mulberry leaf oligosaccharide Characterization Gut microbiota Lactobacillus murinus Hypoglycemic activity

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Distinct Effects of Three Tea Varieties Made Green Teas on Improving Diabetic Dyslipidemia Induced by High-Fat Diet in C57BL/6J Mice

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food Science and Human Wellness 2024年

作者： Wei Gu Zhutong Wang Guohuo Wu Huimin Guo Huijun Cheng Cong Li Linbo Chen Daxiang Li Zhongwen Xie State key laboratory of Tea Plant Biology and Utilization School of Tea and food Sciences & Technology anhui agricultural University Center for Biotechnology anhui agricultural University College of Life Sciences anhui agricultural University Tea Research Institute Yunnan Academy of agricultural Sciences

Currently， cultivated tea plants are classified into three varieties， namely， C. sinensis var. sinensis， C. sinensis var. assamica， and C. sinensis var. kucha. Three tea varieties made green teas (TVGTs) are widely consumed worldwide. However， a comparative investigation of the beneficial effects， mechanism， and characteristic compounds of TVGTs has not been reported. Here， three representative tea plant varieties， namely， C. sinensis var. sinensis cv. Fuding (FD)， C. sinensis var. assamica cv. Yunkang 10 (YK)， and C. sinensis var. kucha (KC)， planted in the same garden， were used to make green teas utilizing the same standard procedure. Our findings show that the TVGTs effectively improved hyperglycemia， obesity， dyslipidemia， fatty liver， and mesenteric artery (MA) hypercontractility in mice with diabetes and obesity induced by a high-fat diet (HFD) after 11- and 22-week interventions， with YK being the most effective at 22 weeks. Liver lipidomics indicated that the TVGTs restored the glycerophospholipid and sphingolipid balance and reduced triglycerides， with YK demonstrating superior effects. The TVGTs， particularly YK， suppressed triglyceride synthesis through the ARV1-FXR-SHP-SREBP-1c pathway. A chemical profile analysis revealed that YK green tea had enriched active compounds， including caffeine， quinic acid， L-theanine， gallic acid， and catechins， compared with FD and KC green teas， which may contribute to ameliorating diabetic dyslipidemia via synergistic action. As a representative tea variety of C. sinensis var. assamica， YK green tea was found to be superior to the other two green teas in preventing and treating diabetic dyslipidemia， especially with long-term consumption. These data provide important information for tea plant breeders and tea consumers.

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Knockout of miR396 genes increases seed size and yield in soybean

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Journal of Integrative Plant Biology 2024年第6期66卷 1148-1157页

作者： Hongtao Xie Fei Su Qingfeng Niu Leping Geng Xuesong Cao Minglei Song Jinsong Dong Zai Zheng Rui Guo Yang Zhang Yuanwei Deng Zhanbo Ji Kang Pang Jian-Kang Zhu Jianhua Zhu School of Life Sciences Anhui Agricultural University Research Center for Biological Breeding Technology Research Institute of Frontier Science Anhui Agricultural University Bellagen Biotechnology Co.Ltd Institute of Crop Sciences/National Nanfan Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Gene Editing Technologies (Hainan) Ministry of Agriculture and Rural Affairs Southern University of Science and Technology Hainan Yazhou Bay Seed laboratory

Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal feed. mi R396 genes have been shown to negatively regulate grain size in rice,but whether mi R396 family members may function in a similar manner in soybean is unknown. Here, we generated eight soybean mutants harboring different combinations of homozygous mutations in the six soybean mi R396genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar Zhonghuang 302(ZH302). Four triple mutants (mir396aci, mir396acd, mir396adf, and mir396cdf), two quadruple mutants(mir396-abcd and mir396acfi), and two quintuple mutants(mir396abcdf and mir396bcdfi) were characterized. We found that plants of all the mir396 mutants produced larger seeds compared to ZH302 plants. Field tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower latitude. In contrast, mir396abcdf and mir396bcdfiplants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues, but they are suited for low latitude growth zones with increased yield without lodging problems.Taken together, our study demonstrated that loss-of-function of mi R396 genes leads to significantly enlarged seed size and increased yield in soybean, providing valuable germplasms for breeding high-yield soybean.

关键词： Cas12SF01 CRISPR/Cas miR396 seed size soybean yield

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The interventional effect of Polygonatum cyrtonema Hua polysaccharide on atherosclerosis in mice of different sexes

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food Science and Human Wellness 2024年第1期13卷 370-380页

作者： Anjun Guo Xueying Li Lihua Pan Qiangming Li Jianping Luo Xueqiang Zha Engineering Research Centre of Bioprocess of Ministry of Education Hefei University of TechnologyHefei 230009China School of food and Biological Engineering Hefei University of TechnologyHefei 230009China key laboratory of Metabolism and Regulation for Major Disease of anhui Higher Education Institutes Hefei University of TechnologyHefei 230009China

In the present study,we investigated the intervention effects of a purified Polygonatum cyrtonema polysaccharide(PCP)on high-fat diet(HFD)-induced atherosclerosis in male and female LDLr-/-mice.Results showed that HFD caused severe dyslipidemia,atherosclerotic lesions,oxidative damages and inflammation in male and female mice,and these effects seemed to be more pronounced in males than in females.However,the above variations could be dose-dependently reversed by PCP treatment,and the intervention effects on males were greater than those on females.Nuclear factor kappa-B(NF-κB),mitogen-activated protein kinase(MAPKs)and protein kinase B(Akt)are 3 pivotal signaling pathways mediating the development of atherosclerosis.Consistently,PCP was also found to significantly decrease the phosphorylation of p65,p38,extracellular-regulated kinase 1/2(ERK1/2)and Akt,and increase the protein expression of inhibitor of NF-κB(IκB)in the aortas of male and female mice induced by HFD.Taken together,these findings indicated that PCP could be effective for the prevention of atherosclerosis,and the intervention effect of PCP on male mice was more obvious than that of female mice.

关键词： Polygonatum cyrtonema polysaccharide Anti-atherosclerosis Sex

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