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Reproductive biology of Hemitripterus villosus in the Bohai Strait,China

Journal of Oceanology and Limnology 2024年第3期42卷 881-892页

作者： Ping SUN Song QIN Zhengyi LIU Longchuan ZHUANG key laboratory of coastal biology and biological resource Utilization Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

To understand the reproductive biology of Hemitripterus villosus in the Bohai Strait,and fill the key knowledge gap in verifying reproductive seasonality and documenting gonadotropin dependent reproductive cycle of this species,a year-round detail study was conducted for the first time.In total,263 individuals were randomly sampled off the coasts of the Bohai Strait.Results show that and the overall female-to-male ratio(1.35:1)did not differ significantly from the 1:1 sex ratio.The length and weight relationship for females and males were regressed,depicting an isometric growth for both sexes.Four reproductive phases of the complete gonadotropin dependent reproductive cycle in both sexes were identified.The monthly variations in the gonadosomatic and hepatosomatic indexes for both sexes further indicated that this species spawns a single batch of eggs per year and the peak spawning period is from October to December.The average diameter of eggs ranged from 2.10 to 3.57 mm.The length at first sexual maturity was 224.87 mm for females and 241.26 mm for males.Significant positive relationships between absolute fecundity and fish size,and also gonadal weight were observed.H.villosus from the Bohai Strait exhibited smaller body size,smaller egg diameter,lower gonadosomatic index,lower absolute fecundity,and higher population extinction risk than the other populations’studied previously.Our findings contribute to understanding the status of this isolated westernmost population,and we emphasize that more efficient protection measures for the species and control measures are urgently required to improve the coastal environment in the Bohai Strait.

关键词： Hemitripterus villosus Bohai Strait fecundity length at first maturity reproductive seasonality

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Alterations in microbial community during the remediation of a black-odorous stream by acclimated composite microorganisms

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Journal of Environmental Sciences 2022年第8期34卷 181-193页

作者： Feng Shi Zongli Liu Jialin Li Huiwang Gao Song Qin Jingjing Guo key lab of coastal biology and biological resource conservation Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Qingdao HOOH Environmental Protection Technology Co.Ltd.Qingdao 266073 China

Microbial application is an efficient, economical, and ecofriendly method for remediating black-odorous rivers. In this study, the field treatment effect and microbial community changes were monitored during remediation by the acclimated complex microorganisms of a typical black-odorous stream. After the treatment, the total phosphorus and ammonia contents decreased by 74.0% and 76.3% and the concentrations of dissolved oxygen increased from 1.65 to 4.90 mg/L, indicating the effectiveness of the acclimated composite microorganisms. The proportion of Bacteroidetes decreased significantly by 48.1% and that of Firmicutes increased by 2.23% on average, and the microbial diversity index first increased and then tended to be uniform. Redundancy analysis demonstrated that the pH,dissolved oxygen, and oxidation-reduction potential together determined the composition of the microbial communities(p < 0.05). These findings showed that the acclimated composite microorganisms can effectively remediate the black odor.

关键词： Black-odorous water Composite microorganisms Microbial community Environmental impact factors In-siu bioremediation

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Distribution and phenogenetic diversity of Synechococcus in the Bohai Sea, China

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Journal of Oceanology and Limnology 2022年第2期40卷 592-604页

作者： Ting WANG Xi CHEN Jialin LI Song QIN College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China key lab of coastal biology and biological resource Utilization Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China College of Marine Life Science Ocean University of ChinaQingdao 266005China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Synechococcus is one of the most abundant picocyanobacteria in marine ecosystem,and the absence of Prochlorococcus would make it indispensable as a primary producer in the Bohai Sea,North China.However,the abundance distribution and genetic diversity of Synechococcus in this region have rarely been reported.In this study,the distribution pattern of Synechococcus abundance was investigated during four cruises in April,June,August,and November from 2018 to 2019,moreover,its phenogenetic diversity was studied based on high-throughput sequencing of the cpe BA operon.The results demonstrate that phycoerythrin-containing Synechococcus was most abundant in August when temperature was high and oxygen saturation was low.During this period,Synechococcus pigment type(PT)2 was abundant in the Bohai Bay and Laizhou Bay under conditions of high nutrient concentration,temperature,and turbidity.In comparison,PT3,especially those clusters characterized with high or variable ratio of phycourobilin and phycoerythrobilin,was predominant in the Bohai Strait and Liaodong Bay under conditions of high salinity,pH,and oxygen saturation.Furthermore,co-occurrence correlations using network analysis revealed that Synechococcus PTs were related to 15.37%-43.48%of the prokaryotic genera.Synechococcus PT3c/PT3d and PT2 were the most important PTs in the network.The hierarchical clustering revealed that taxa co-occurred with Synechococcus PTs diff ered among samples.It could be attributed to the substance exchange and the environmental impact,which calls for more studies in the future.

关键词： Synechococcus phenogenetic diversity co-occurrence network coastal ecosystem Bohai Sea

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Transient expression of the enhanced green fluorescent protein(egfp) gene in Sargassum horneri

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Journal of Oceanology and Limnology 2019年第2期37卷 651-656页

作者： PANG Yunlong LI Yan LIU Zhengyi CUI Yulin QIN Song key laboratory of coastal biology and biological resource Utilization Yantai Institute of Coastal Zone Research Chinese Academy of Sciences Yantai 264003 China University of Chinese Academy of Sciences Beijing 101408 China Yantai Ocean Environmental Monitoring Central Station of State Oceanic Administration Yantai 264006 China

Sargassum horneri is a macroalga widespread in North Asia-Pacific region, and these years its bloom has caused huge damage to the environment and the economic in China. To make up the blank on genetic engineering research, a transient transformation system for the multicellular marine brown alga S . horneri was established in this research. The algae used in this research were collected from the Yellow Sea of China and verified as a same species S . horneri with analysis of molecular markers. The S . horneri parietal leaves were transformed with the enhanced green fluorescent gene as the reporter by micro-particle bombardment. The results show that green fluorescent protein (GFP) is an eff ective transgene reporter for S . horneri and that particle bombardment is a suitable method for transformation of S . horneri . Through selection of four diff erent promoters for EGFP and six groups’ bombardment characters, the highest transformation efficiency approximately 1.31% was got with the vector pEGFP-N1 at bombardment characters 900 spi and 6 cm distance. This research paves a way for the further research and application of S . horneri .

关键词： green fluorescent protein (GFP) particle bombardment Sargassum horneri transgenesis

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Temporal Dynamics and Performance Association of the Tetrasphaera-Enriched Microbiome for Enhanced biological Phosphorus Removal

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Engineering 2023年第10期29卷 168-178页

作者： Hui Wang Yubo Wang Guoqing Zhang Ze Zhao Feng Ju College of Environmental and resource Sciences Zhejiang UniversityHangzhou 310012China key laboratory of coastal Environment and resources of Zhejiang Province School of EngineeringWestlake UniversityHangzhou 310024China Institute of Advanced Technology Westlake Institute for Advanced StudyHangzhou 310024China Center of Synthetic biology and Integrated Bioengineering School of EngineeringWestlake UniversityHangzhou 310024China Research Center for Industries of the Future Westlake UniversityHangzhou 310030China

Tetrasphaera have been recently identified based on the 16S ribosomal RNA(rRNA)gene as among the most abundant polyphosphate-accumulating organisms(PAOs)in global full-scale wastewater treatment plants(WWTPs)with enhanced biological phosphorus removal(EBPR).However,it is unclear how Tetrasphaera PAOs are selectively enriched in the context of the EBPR microbiome.In this study,an EBPR microbiome enriched with Tetrasphaera(accounting for 40%of 16S sequences on day 113)was built using a top-down design approach featuring multicarbon sources and a low dosage of allylthiourea.The microbiome showed enhanced nutrient removal(phosphorus removal~85%and nitrogen removal~80%)and increased phosphorus recovery(up to 23.2 times)compared with the seeding activated sludge from a local full-scale WWTP.The supply of 1 mg·L^(-1)allylthiourea promoted the coselection of Tetrasphaera PAOs and Microlunatus PAOs and sharply reduced the relative abundance of both ammonia oxidizer Nitrosomonas and putative competitors Brevundimonas and Paracoccus,facilitating the establishment of the EBPR microbiome.Based on 16S rRNA gene analysis,a putative novel PAO species,EBPR-ASV0001,was identified with Tetrasphaera japonica as its closest relative.This study provides new knowledge on the establishment of a Tetrasphaera-enriched microbiome facilitated by allylthiourea,which can be further exploited to guide future process upgrading and optimization to achieve and/or enhance simultaneous biological phosphorus and nitrogen removal from high-strength wastewater.

关键词： Enhanced biological phosphorus removal(EBPR) Polyphosphate-accumulating organisms(PAOs) Tetrasphaera Microbiome Phosphorus recovery

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Ergochaeglobosins A—E, Unprecedented Heterodimers of Cytochalasan and Ergosterol from Chaeglobosin globosum P2-2-2

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Chinese Journal of Chemistry 2022年第16期40卷 1909-1916页

作者： Xiaogang Peng Junjun Liu Chunlun Qin Qian Wu Wanpeng Li Fatemeh Mohammadipanah Hanli Ruan School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyHubei Key Laboratory of Natural Medicinal Chemistry and Resource EvaluationWuhanHubei430030 China Pharmaceutical Biotechnology lab School of Biology and Center of Excellence in Phylogeny of Living OrganismsCollege of ScienceUniversity of Tehran14155-6455TehranIran

Five novel heterodimers of cytochalasan and ergosterol,ergochaeglobosins A—E,were isolated from an endophytic fungus Chaetomium globosum P2-2-2.Ergochaeglobosin A(1) possesses an unprecedented 5/6/13/6/5/6/6/6 fused octacyclic ring system,and ergochaeglobosins B(2) and C(3) form a unique 5/6/13/6/6/6/6/5 fused octacyclic ring system,whereas ergochaeglobosins D(4) and F(5) are the first cytochalasan heterodimers formed via a N-1′/C-3′′single bond.Their structures with absolute configurations were established by extensive spectroscopic analyses,single-crystal X-ray diffraction,and ECD calculations.The plausible biogenetic pathway of 1—5 was postulated.Compound 1 exhibited inhibitory effects against ConA-induced T cells and LPS-induced B cells proliferation with IC50 values of 19.2 and 14.1μmol/L,respectively.Co-treatment of 3(20μmol/L)with TRAIL(150 ng/mL)could significantly decrease the cell viability of A549 by 24.9%comparing with monotherapy of 3(20μmol/L).

关键词： Cytochalasan heterodimers Natural products biological activity Chaeglobosin globosum P2-2-2 Structure elucidation

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Deep proteome profiling promotes whole proteome characterization and drug discovery for esophageal squamous cell carcinoma

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Cancer biology & Medicine 2022年第3期19卷 273-277页

作者： Wei Liu Yongping Cui Wen Liu Zhihua Liu Liyan Xu Enmin Li College of Science Heilongjiang Institute of TechnologyHarbin 150050China Guangdong Provincial key laboratory of Infectious Diseases and Molecular Immunopathology the Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan AreaShantou University Medical CollegeShantou 515041China Department of Pathology Key Laboratory of Cellular Physiology(Shanxi Medical University)Ministry of EducationTaiyuan 030001China Fujian Provincial key laboratory of Innovative Drug Target Research School of Pharmaceutical SciencesXiamen UniversityXiamen 361005China State key lab of Molecular Oncology National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Proteins are key players in various cellular processes.As the ultimate executors of cellular processes,they play essential roles in linking genotypes to phenotypes.Abnormalities in protein expression and post-translational modifications(PTMs)are closely associated with the initiation and development of cancer,and they carry biological information inaccessible to genomics and transcriptomics1.Proteomics complements genomic and transcriptomic data,thus enabling comprehensive analysis of cancer pathogenesis and accelerating biomedical research2.

关键词： esophageal linking carry

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Trophic gauntlet effects on fisheries recovery:a case study in Sansha Bay,China

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Ecosystem Health and Sustainability 2021年第1期7卷 318-331页

作者： Bin Xie Cheng Huang Ya Wang Xijie Zhou Guogan Peng Yancheng Tao Jiaju Huang Xiaoqing Lin Lingfeng Huang key laboratory of the Ministry of Education for coastal and Wetland Ecosystems College of the Environment and EcologyXiamen UniversityXiamen China Fujian Provincial key laboratory of coastal Ecology and Environmental Studies Xiamen UniversityXiamen China Guangxi key lab of Mangrove conservation and Utilization Guangxi Academy of SciencesBeihai China

Low biological productivity causes ecosystem energy deficiency.Sansha Bay is an important spawning and nursery ground for migratory fish in the East China Sea(ECS).There is significant mariculture in this area,but stock enhancement programs and seasonal fishery closures have failed to recover populations of the commercially important species,Larimichthys crocea.We want to figure out the potential reason of unsuccessful L.crocea resource in recent 30 years.In this study,the trophic status of L.crocea,the food source proportions of L.crocea and zooplankton,and the food web structure and functioning of Sansha Bay was analyzed.A high nutrient low chlorophyll phenomenon was observed:this prevented harmful algal blooms,and phytoplankton growth was restrained by intensive macroalgal culture,resulting in a low abundance of zooplankton in Sansha Bay.Phytoplankton was the most important food source of zooplankton,and zooplankton was the greatest food source of juvenile L.crocea.Analyzed L.crocea suffered from starvation.Crucially,most of the phytoplankton was not used efficiently in the Sansha Bay ecosystem.This study suggests that trophic bottleneck,caused by food limitation,is a potential reason for unsuccessful fishery recovery in enclosed bay with macroalgal culture.

关键词： High nutrient low chlorophyll ecopath stable isotope mariculture Sansha Bay Larimichthys crocea

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Progress and prospects of noncoding RNAs in insects

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Journal of Integrative Agriculture 2019年第4期18卷 729-747页

作者： LI Mei-zhen XIAO Hua-mei HE Kang LI Fei Ministry of Agriculture key lab of Molecular biology of Crop Pathogens and Insects Institute of Insect Sciences College of Agriculture and Biotechnology Zhejiang University College of Life Sciences and resource Environment Yichun University

With the rapid development of high-throughput sequencing technology and bioinformatics algorithms, great progress has been made in the field of noncoding RNA(ncRNA) in the last decade. RNA molecules have been regarded only as a messenger between DNA and protein for decades, but now they have new roles in the biological process as ncRNAs. A growing number of ncRNAs have been identified in insects from the RNA-Seq data of small RNA libraries or transcriptomes. ncRNAs have varied regulatory functions at the epigenetic, transcriptional, or post-transcriptional levels, and participate in almost all types of biological processes. Here, we review the research progress of four kinds of ncRNAs, including microRNA(miRNA), Piwiinteracting RNA(piRNA), circular RNA(circRNA), and long noncoding RNA(lncRNA) in insects. The discovery, biogenesis mechanisms, and regulatory functions of these ncRNAs are presented here to provide a comprehensive understanding of insect ncRNAs and to promote the application of ncRNAs in insect pest control.

关键词： miRNA piRNA circRNA IncRNA biogenesis prediction regulatory functions

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Isolation of a genomic DNA for Jatropha curcas ribosome inactivating protein and its tobacco transformation

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Journal of Shanghai University(English Edition) 2006年第5期10卷 461-464页

作者：罗言云魏琴黄明星徐莺陈放 Sichuan key lab of resource biology and Pharmaceutical Engineering College of Life Sciences Sichuan University Chengdu 610064 P.R. China Sichuan key lab of resource biology and Pharmaceutical Engineering College of Life Sciences Sichuan University Chengdu 610064 P.R. ChinaDepartment of Biological Engineering Yibin University Yinbin 644007 P.R. China

Genomic DNA for Jatropha curcas ribosome inactivating protein （JRIP） was cloned from total DNA of its leaves by polymerase chain reaction （PCR）. The no intron character was confirmed. The plant expression vector pBI121-JRIP was constructed by inserting the JRIP gene into pBI121 plasmid. The recombinant Agrobacterium EHA105 strain harboring pBI121-JRIP was constructed by conducting pBI121-JRIP to strain EHA 105. PCR and Southern blotting were carried out, and the results proved that the JRIP gene was integrated into tobacco genome. It might provide a new material for disease resistance tobacco species breeding.

关键词： Jatropha curcas tobacco transformation intron ribosome inactivating protein.

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