Temporal Dynamics and Performance Association of the Tetrasphaera-Enriched Microbiome for Enhanced Biological Phosphorus Removal
作者机构：College of Environmental and Resource SciencesZhejiang UniversityHangzhou 310012China Key Laboratory of Coastal Environment and Resources of Zhejiang ProvinceSchool of EngineeringWestlake UniversityHangzhou 310024China Institute of Advanced TechnologyWestlake Institute for Advanced StudyHangzhou 310024China Center of Synthetic Biology and Integrated BioengineeringSchool of EngineeringWestlake UniversityHangzhou 310024China Research Center for Industries of the FutureWestlake UniversityHangzhou 310030China
出 版 物：《Engineering》 (工程（英文）)
年 卷 期：2023年第29卷第10期
页 面：168-178页
核心收录：
学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学]
基 金：supported by the Key Research and Development Program of Zhejiang(2022C03075) National Natural Science Foundation of China(22241603) Zhejiang Provincial Natural Science Foundation of China(LR22D010001)
主 题：Enhanced biological phosphorus removal(EBPR) Polyphosphate-accumulating organisms(PAOs) Tetrasphaera Microbiome Phosphorus recovery
摘 要：Tetrasphaera have been recently identified based on the 16S ribosomal RNA(rRNA)gene as among the most abundant polyphosphate-accumulating organisms(PAOs)in global full-scale wastewater treatment plants(WWTPs)with enhanced biological phosphorus removal(EBPR).However,it is unclear how Tetrasphaera PAOs are selectively enriched in the context of the EBPR *** this study,an EBPR microbiome enriched with Tetrasphaera(accounting for 40%of 16S sequences on day 113)was built using a top-down design approach featuring multicarbon sources and a low dosage of *** microbiome showed enhanced nutrient removal(phosphorus removal~85%and nitrogen removal~80%)and increased phosphorus recovery(up to 23.2 times)compared with the seeding activated sludge from a local full-scale *** supply of 1 mg·L^(-1)allylthiourea promoted the coselection of Tetrasphaera PAOs and Microlunatus PAOs and sharply reduced the relative abundance of both ammonia oxidizer Nitrosomonas and putative competitors Brevundimonas and Paracoccus,facilitating the establishment of the EBPR *** on 16S rRNA gene analysis,a putative novel PAO species,EBPR-ASV0001,was identified with Tetrasphaera japonica as its closest *** study provides new knowledge on the establishment of a Tetrasphaera-enriched microbiome facilitated by allylthiourea,which can be further exploited to guide future process upgrading and optimization to achieve and/or enhance simultaneous biological phosphorus and nitrogen removal from high-strength wastewater.
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