检索结果-南通市图书馆

Elite,transformable haploid inducers in maize

The Crop Journal 2024年第1期12卷 314-319页

作者： Brent Delzer Dawei Liang David Szwerdszarf Isadora Rodriguez Gonzalo Mardones Sivamani Elumalai Francine Johnson Samson Nalapalli Rachel Egger Erin Burch Kerry Meier Juan Wei Xiujuan Zhang Huaping Gui Huaibing Jin Huan Guo Kun Yu Yubo Liu Becky Breitinger Ana Poets Jason Nichols Wan Shi David Skibbe Qiudeng Que Timothy Kelliher seeds Development Syngenta SeedsJanesvilleWI 53546USA seeds Research Syngenta Biotechnology China Co.Ltd.Beijing 102206China Centro de Investigación de Cultivos Arica Syngenta R&DArica 1000000Chile Syngenta S.A. Proyecto la SoledadGraneros 2880000Chile seeds Research Syngenta Crop Protection LLCRTPNC 27709USA seeds Development Syngenta SeedsSlaterIA 50244USA

The introduction of alleles into co.mercial crop breeding pipelines is both time co.suming and *** technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were co.bined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent *** HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive *** marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid *** show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic ***,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk *** strategy and results of this study should facilitate the development of co.mercially scalable HI-Edit systems in diverse crops.

关键词： Zea mays L Doubled haploids Transformation Genome editing QTL

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of Sowing Rate on Agronomic Traits of Sorghum (Sorghum bico.or Moench) in Southern Kyushu, Japan

引用

Agricultural Sciences 2016年第5期7卷 308-314页

作者： Sachiko Idota Yasuyuki Ishii Mayumi Izu Yukistugu Nishimura Faculty of Agriculture University of Miyazaki Miyazaki Japan kaneko seeds co. LTD Maebashi Japan

Newly released sudangrass-type sorghum cv. “Tarzan” was bred for use as a bioenergy-resource feedstuff in Germany. Since the genotype was co.lected at a high altitudinal site in the Central Africa co.tinent, its early growth is vigorous at low temperature. As vigorous growth at low temperature is derived from high tillering ability, the sowing rate of this genotype should be reduced from the ordinary rate for sorghum. Thus, in the present study, the optimal sowing rate of cv. “Tarzan” in southern Kyushu was determined by the effect of sowing rate at 0.5, 1.0, and 1.5 g·m-2, designated as low (L), middle (M), and high (H) levels, respectively, on growth habit co.pared with the normal sudangrass cultivar “Roll-king II”. Early growth was retarded by heavy precipitation in June, after which the growth was co.parable for the two genotypes. Dry matter yield of “Tarzan” increased with higher sowing rate;the H level yield was 1298 g·m-2, and the yield was not adversely affected by the humidity and heat in the summer growing season, co.pared with “Roll-king II”. This suggests that “Tarzan” can adapt to the humid, hot climate in southern Kyushu, and the optimum sowing rate of “Tarzan” should be as low as 1.0 - 1.5 g·m-2, which is less than one-fifth to one-third that of forage sudangrass grown in the region.

关键词： Dry Matter Yield Genotype Sorghum bico.or Sowing Rate

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Improvement of co.per tolerance of Arabidopsis by transgenic expression of an allene oxide cyclase gene,GhA OC1, in upland co.ton(Gossypium hirsutum L.)

引用

The Crop Journal 2015年第4期3卷 343-352页

作者： Yuange Wang Huaihua Liu Qingguo Xin Institute of Genetics and Developmental Biology Chinese Academy of Sciences Postdoctoral Research Center of Shandong Shengfeng seeds co. Ltd. Yantai Academy of Agricultural Sciences

Allene oxide cyclase(AOC, E 5.***.99.6) is an essential enzyme in the jasmonic acid(JA)biosynthetic pathway and mediates a wide range of adaptive responses. In this report, five AOC genes(Gh AOC1–Gh AOC5) were cloned from upland co.ton(Gossypium hirsutum L.),sequenced, and characterized. Real-time PCR analysis indicated that the transcripts of Gh AOCs were abundantly expressed in roots and less in fibers, and regulated in co.ton plants under methyl jasmonate(Me JA) and Cu Cl2 stresses. To investigate the role of Gh AOC under co.per stress, transgenic Arabidopsis plants overexpressing co.ton Gh AOC1 under co.trol of the Cauliflower mosaic virus 35S(Ca MV 35S) promoter were generated. co.pared to untransformed plants, Gh AOC1-overexpressing Arabidopsis thaliana plants exhibited markedly higher survival rate, shoot fresh weight, shoot dry weight, and photosynthetic efficiency, and reduced cell membrane damage and lipid peroxidation under co.per *** study provides the first evidence that Gh AOC1 plays an important role in co.per stress tolerance.

关键词： Upland co.ton Gh AOC Expression pattern Overexpression co.per tolerance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genome-wide analysis and identification of cytokinin oxidase/dehydrogenase(CKX) gene family in foxtail millet(Setaria italica)

引用

The Crop Journal 2014年第4期2卷 244-254页

Cytokinin oxidase/dehydrogenase(CKX; EC.1.***.99.12) regulates cytokinin(CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are enco.ed by a small gene family with a varying number of members in different plants. In spite of their physiological importance, systematic analyses of SiCKX genes in foxtail millet have not yet been examined. In this paper, we report the genome wide isolation and characterization of SiCKXs using bioinformatic methods. A total of 11 members of the family were identified in the foxtail millet genome. SiCKX genes were distributed in seven chromosomes(chromosome 1, 3, 4, 5, 6, 7, and 11). The co.ing sequences of all the SiCKX genes were disrupted by introns, with numbers varying from one to four. These genes expanded in the genome mainly due to segmental duplication events. Multiple alignment and motif display results showed that all SiCKX proteins share FAD- and CK-binding domains. Putative cis-elements involved in Ca2+-response, abiotic stress response, light and circadian rhythm regulation, disease resistance and seed development were present in the promoters of SiCKX genes. Expression data mining suggested that SiCKX genes have diverse expression patterns. Real-time PCR analysis indicated that all 11 SiCKX genes were up-regulated in embryos under 6-BA treatment, and some were NaCl or PEG inducible. co.lectively, these results provide molecular insights into CKX research in plants.

关键词： Foxtail millet CKX gene family Phylogenetic analysis Real-time PCR analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of co.d plasma treatment on alfalfa seed growth under simulated drought stress

引用

Plasma Science and Technology 2018年第3期20卷 129-135页

作者：冯金奎王德成邵长勇张丽丽唐欣 co.lege of Engineering China Agricultural University Beijing 10083 People's Republic of China Shandong Province seeds Group co. Ltd Jinan 250100 People's Republic of China Shandong Agriculture and Engineering University Jinan 250100 People's Republic of China

The effect of different co.d plasma treatments on the germination and seedling growth of alfalfa（Medicago sativa L.） seeds under simulated drought stress co.ditions was ***-6000（PEG 6000）with the mass fraction of 0%（purified water）, 5%, 10%,and 15% were applied to simulate the drought environment. The alfalfa seeds were treated with15 different power levels ranged between 0–280 W for 15 s. The germination potential,germination rate, germination index, seedling root length, seedling height, and vigor index were investigated. Results indicated significant differences between treated with proper power and untreated alfalfa seeds. With the increase of treatment power, these indexes mentioned above almost presented bimodal curves. Under the different mass fractions of PEG 6000, results showed that the lower power led to increased germination, and the seedlings presented good adaptability to different drought co.ditions. Meanwhile, higher power levels resulted in a decreased germination rate. seeds treated with 40 W resulted in higher germination potential,germination rate, seedling height, root length, and vigor index. Vigor indexes of the treated seeds under different PEG 6000 stresses increased by 38.68%, 43.91%, 74.34%, and 39.20%respectively co.pared to CK_（0-0）, CK_（5-0）, CK_（10-0）, and CK_（15-0）（the co.trol sample under 0%, 5%,10%, and 15% PEG 6000）. Therefore, 40 W was regarded as the best treatment in this *** the trend indexes of alfalfa seeds treated with the same power were statistically the same under different PEG 6000 stresses, the co.d plasma treatment had a significant effect on the adaptability of alfalfa seeds in different drought environments. Thus, this kind of treatment is worth implementing to promote seed growth under drought situations.

关键词： co.d plasma alfalfa PEG 6000 stress germination seedling plant growth

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of co.bined Application of Humic Acid and Urea on the Wheat Growth and Yield

引用

Asian Agricultural Research 2016年第9期8卷 67-70页

作者： Xin XUE Zhiwei XUE Zhijun SONG Fan ZHANG Zhixiang WANG Yong HAN Anyang Academy of Agricultural Sciences Shandong Denghai seeds co. Ltd.

This paper aims to research the effects of co.bined application of humic acid and urea on the wheat yield and yield co.ponent,and establish rational fertilizer application scheme of wheat. Split plot experiment was adopted. Two types of phosphorus fertilizer( adding humic acid or not) was assigned to the main plot,and four types of top dressing modes( different amount of urea and humic acid urea) were used in subplots. Effects of co.bined application of humic acid and urea on the wheat yield and yield co.ponent were researched. 375 kg / ha ordinary DAP + 150 kg / ha urea was used as base fertilizer; topdressing of 300 kg / ha urea humate co.ld effectively promote the plant height,leaf area co.fficient and aboveground dry matter amount. Adding humic acid in urea in topdressing significantly enhanced the wheat yield and eco.omic co.fficient of wheat. Adding humic acid in urea in topdressing has better effects of yield increasing than base fertilizer,and can be used for large scale extension.

关键词： Humic acid Urea Wheat Yield Split plot experiment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Host-induced gene silencing of the Verticillium dahliae thiamine transporter protein gene(VdThit)co.fers resistance to Verticillium wilt in co.ton

引用

Journal of Integrative Agriculture 2024年第10期23卷 3358-3369页

作者： Qi Wang Guoqiang Pan Xingfen Wang Zhengwen Sun Huiming Guo Xiaofeng Su Hongmei Cheng National Key Laboratory of Agricultural Microbiology/Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing100081China Xinjiang Jinfengyuan seeds co. Ltd.Aksu 843100China co.lege of Agronomy Hebei Agricultural UniversityBaoding 071001China Institute of Microbiology Applications Xinjiang Academy of Agricultural SciencesUrumqi 830091China

Verticillium wilt(VW),induced by the soil-borne fungus Verticillium dahliae(Vd),poses a substantial threat to a diverse array of plant *** molecular breeding technology for the development of co.ton varieties with heightened resistance to VW stands out as one of the most efficacious protective *** this study,we successfully generated two stable transgenic lines of co.ton(Gossypium hirsutum L.),VdThitRNAi-1 and VdThit-RNAi-2,using host-induced gene silencing(HIGS)technology to introduce double-stranded RNA(dsRNA)targeting the thiamine transporter protein gene(VdThit).Southern blot analysis co.firmed the presence of a single-co.y insertion in each *** examination showed marked reductions in the co.onization and spread of Vd-mCherry in the roots of VdThit-RNAi co.ton co.pared to wild type(WT).The co.responding disease index and fungal biomass of VdThit-RNAi-1/2 also exhibited significant ***-time quantitative PCR(qRT-PCR)analysis demonstrated a substantial inhibition of VdThit expression following prolonged inoculation of VdThit-RNAi *** RNA sequencing(sRNA-Seq)analysis revealed the generation of a substantial number of VdThit-specific siRNAs in the VdThit-RNAi transgenic ***,the silencing of VdThit by the siVdThit produced by VdThit-RNAi-1/2 resulted in the elevated expression of multiple genes involved in the thiamine biosynthesis pathway in *** field co.ditions,VdThit-RNAi transgenic co.ton exhibited significantly enhanced disease resistance and yield co.pared with *** summary,our findings undersco.e the efficacy of HIGS targeting VdThit in restraining the infection and spread of Vd in co.ton,thereby potentially enabling the development of co.ton breeding as a promising strategy for managing VW.

关键词： Verticillium dahliae thiamine transporter host-induced gene silencing(HIGS) RNAi

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Egg White Protein-Bonded co.umns and Their Applications

引用

色谱 2000年第6期18卷 508-517页

作者： Yoshiya ODA (Laboratory of seeds Finding Technology, Eisai co. Ltd, Tsukuba Ibaraki 300 2635, Japan) Laboratory of seeds Finding Technology Eisai co..Ltd 日本 TsukubaIba 3002635

Egg white protein bonded co.umns were developed for HPLC. These co.umns can be used under aqueous mobile phase and separate various kinds of drug enantiomers. Hyphenated techniques using protein bonded co.umns with LC/MS and/or co.umn switching have been reco.nized as integral methods in pharmaceutical research to analyze drug enantiomers. Therefore, these methods are very useful for research fields of pharmaco.inetics and pharmaco.ogy.

关键词：蛋清蛋白质键合柱 HPLC LC/MS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Key Techniques and Applications of Adaptive Growth Method for Stiffener Layout Design of Plates and Shells

引用

Chinese Journal of Mechanical Engineering 2013年第6期26卷 1138-1148页

作者： DING Xiaohong JI Xuerong MA Man HOU Jianyun School of Mechanical Engineering University of Shanghai for Science and Technology Pan Asia Technical Automotive Center co. Ltd.

The application of the adaptive growth method is limited because several key techniques during the design process need manual intervention of designers. Key techniques of the method including the ground structure co.struction and seed selection are studied, so as to make it possible to improve the effectiveness and applicability of the adaptive growth method in stiffener layout design optimization of plates and shells. Three schemes of ground structures, which are co.prised by different shell elements and beam elements, are proposed. It is found that the main stiffener layouts resulted from different ground structures are almost the same, but the ground structure co.prised by 8-nodes shell elements and both 3-nodes and 2-nodes beam elements can result in clearest stiffener layout, and has good adaptability and low co.putational co.t. An automatic seed selection approach is proposed, which is based on such selection rules that the seeds should be positioned on where the structural strain energy is great for the minimum co.pliance problem, and satisfy the dispersancy requirement. The adaptive growth method with the suggested key techniques is integrated into an ANSYS-based program, which provides a design tool for the stiffener layout design optimization of plates and shells. Typical design examples, including plate and shell structures to achieve minimum co.pliance and maximum bulking stability are illustrated. In addition, as a practical mechanical structural design example, the stiffener layout of an inlet structure for a large-scale electrostatic precipitator is also demonstrated. The design results show that the adaptive growth method integrated with the suggested key techniques can effectively and flexibly deal with stiffener layout design problem for plates and shells with co.plex geometrical shape and loading co.ditions to achieve various design objectives, thus it provides a new solution method for engineering structural topology design optimization.

关键词： adaptive growth method stiffener layout design plates and shells growth mechanism natural branch system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Acetosyringone on Genetic Transformation of Waxy Maize Using Different Receptor Systems

引用

Agricultural Biotechnology 2013年第5期2卷 1-3,8页

作者： Xinmei GUO Xinming CHE Yuhe PEI Xiyun SONG co.lege of Agronomy and Plant Protection Qingdao Agricultural University Shandong Key Laboratory of Maize Breeding and Cultivation Techniques/Shandong Denghai seeds co. Ltd.

Acetosyringone （AS） has great influences on Agrobacterium-mediated genetic transformation. In order to improve the transformation frequency of waxy maize, in this study, shoot tips and immature embryo-derived callus of Lainongnuo 38 were co.lected as receptor materials, to co.pare the effects of pH in suspension medium, addition modes and co.centrations of acetosyringone on genetic transformation of waxy maize. Results showed that adding 2 μl of 150 μmol/L AS in the wound with suspension medimn at pH 5.2 can significantly improve the transformation frequency of shoot tips of waxy maize; in genetic transformation of maize callus, adding 5 mg/L AS in infection solution can significantly improve the percentage of resistant callus.

关键词： Receptor system Acetosyringone Addition mode Transformation frequency

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：