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Tritrichomonas foetus:New structures by high-resolution scanning helium ion microscopy

BIOCeLL 2021年第2期45卷 259-266页

作者： MARLeNe BeNCHIMOL ABIGAIL MIRANDA-MAGALHÃeS ANTONIO PeReIRA-NeVeS WANdeRLeY de SOUZA Universidade do Grande Rio Duque de Caxias25071-202Rio de Janeiro departamento de Microbiologia Laboratório de Biologia Celular de PatógenosFiocruzInstituto Aggeu MagalhãesRecife52060-269Brazil Universidade Federal do Rio de Janeiro Instituto de Biofísica Carlos Chagas FilhoLaboratório de Ultraestrutura Celular Hertha Meyer Instituto Nacional de Ciência e Tecnologia(INBEB)and Centro Nacional de Biologia Estrutural e Bioimagens(CENABIO)Rio de Janeiro21941-902Brazil

Helium ion scanning microscopy(HIM)is a novel high-resolution scanning microscopy technique that uses helium ions instead of electrons to form images of the highest quality and resolution,providing a sub-nanometer resolution sputter uncoated biological ***,we took advantage of HIM to explore the cell surface of Tritrichomonas foetus,a protist parasite of cattle that provokes hard infection and abortion in *** describe thin protrusions,like nanotubes described in other cells,with different sizes(27 nm to 81 nm in thickness)and various lengths(from 73 nm to 2μm),as well bulbous structures either budding from the cell surface or present in the extremities of some *** flagella also presented these thin protrusions and different protein decoration,similar to those previously described using freeze-fracture *** between two cells were also seen,and their role in infection is *** cell surface was also examined and showed several pits indicative of endocytic activity and other types of arrays of *** observations were confirmed using Scanning electron Microscopy(Sem),negative staining,and conventional thin sectioning for observation by transmission electron *** findings provide new and relevant information that may contribute to a better understanding of protozoan biology and its interaction with mammalian cells.

关键词： Nanotubes Microvesicles Surface specializations

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Trypanosoma cruzi invasion in non-phagocytic cells:an ultrastructural study

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BIOCeLL 2018年第3期42卷 105-108页

作者： Juan Agustín CUeTO emile SANTOS BARRIAS Wanderley de SOUZA Patricia Silvia ROMANO IHem Universidad Nacional de CuyoCONICETLaboratorio de Biología de Tripanosoma cruzi y de la célula hospedadoraMendozaArgentina Universidad nacional de Cuyo.Facultad de ciencias Médicas.Área de Biología Celular y Molecular.Mendoza.Argentina Universidad nacional de Cuyo.Facultad de ciencias Médicas.instituto de Fisiología.Mendoza.Argentina Laboratório de Ultraestrutura Celular Hertha Meyer Instituto de Biofísica Carlos Chagas FilhoCentro de Ciência da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil instituto nacional de ciência e tecnologia em biologia estrutural e bioimagens e centro nacional de bioimagens-cenabio Universidade Federal do Rio de JaneiroRio de JaneiroBrazil instituto nacional de Metrologia Qualidade e Tecnologia-InmetroRio de JaneiroBrazil

Trypanosoma cruzi is the causative agent of Chagas *** parasite requires the intracellular niche in order to proliferate and disseminate the *** invasion,*** resides temporarily in an acidic vacuole which is lysed by a not well-understood *** electron microscopy was used to describe the process of *** escape from the parasitophorous vacuole over the *** HeLa(non-professional phagocytic cells)as host cell,we observed that recently internalized parasites reside in a membrane-bounded vacuole.A few hours later,the first sign of vacuole disruption appeared as membrane *** observation was followed by a progressive vacuole swelling as evidenced by an electron-lucent halo between the parasite and the vacuole ***,the vacuole membrane remnants reorganized as small vesicles that eventually disappeared from the vicinity of the ***,parasites reach the host cell cytosol where replication takes *** thorough ultrastructural description of this process set the base for a comprehensive understanding of the parasite-host cell interaction and,thus open the possibility of new therapeutic intervention strategies.

关键词： Parasitophorous vacuole Pathogen Parasites Chagas disease Infectious diseases

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Mechanobiology of the cell surface:Probing its remodeling dynamics using membrane tether pulling assays with optical tweezers

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BIOCeLL 2022年第9期46卷 2009-2013页

作者： JULIANA SOAReS DOUGLAS G.FReITAS PeDRO S.LOUReNÇO JeFTe FARIAS BRUNO PONTeS centro nacional de biologia estrutural e Bioimagem-cenabio Universidade Federal do Rio de JaneiroRio de Janeiro21941-902Brazil Programa de Pós-graduação em ciências Biológicas Biofísica Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de Janeiro21941-902Brazil Programa de Pós-graduação Multidisciplinar em Física Aplicada Instituto de FísicaUniversidade Federal do Rio de JaneiroRio de Janeiro21941-972Brazil instituto de ciências Biomédicas Universidade Federal do Rio de JaneiroRio de Janeiro21941-902Brazil Programa de Pós-graduação em Biotecnologia Instituto de Ciências BiológicasUniversidade Federal do AmazonasManaus69067-005Brazil

Mammalian cell surfaces consist of the plasma membrane supported by an underneath cortical ***,these structures can control not only the shape of cells but also a series of cellular functions ranging from migration and division to exocytosis,endocytosis and ***,the cell surface is capable of exerting and reacting to mechanical *** viscoelastic properties,especially membrane tension and bending modulus,are fundamental parameters involved in these *** viewpoint summarizes our current knowledge on how to measure the viscoelastic properties of cell surfaces employing optical tweezers-based tether assays,paving the way for a better understanding of how cells react to external mechanical forces,with a glance on their remodeling dynamics and possible consequences on downstream cellular processes.

关键词： Cell surface architecture Actin cortex Membrane-cytoskeleton complex Optical tweezers Tether extraction

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