Trypanosoma cruzi invasion in non-phagocytic cells:an ultrastructural study

作     者：Juan Agustín CUETO Emile SANTOS BARRIAS Wanderley de SOUZA Patricia Silvia ROMANO 

作者机构：IHEMUniversidad Nacional de CuyoCONICETLaboratorio de Biología de Tripanosoma cruzi y de la célula hospedadoraMendozaArgentina Universidad Nacional de Cuyo.Facultad de Ciencias Médicas.Área de Biología Celular y Molecular.Mendoza.Argentina Universidad Nacional de Cuyo.Facultad de Ciencias Médicas.Instituto de Fisiología.Mendoza.Argentina Laboratório de Ultraestrutura Celular Hertha MeyerInstituto de Biofísica Carlos Chagas FilhoCentro de Ciência da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens e Centro Nacional de Bioimagens-CENABIOUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto Nacional de MetrologiaQualidade e Tecnologia-InmetroRio de JaneiroBrazil 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2018年第42卷第3期

页      面：105-108页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was financed by grants from Universidad Nacional de Cuyo to JAC and PSR(J043 and J481) Agencia Nacional de Promoción Científica y Tecnológica PICT 2013-2757 to PSR 

主　　题：Parasitophorous vacuole Pathogen Parasites Chagas disease Infectious diseases 

摘      要：Trypanosoma cruzi is the causative agent of Chagas *** parasite requires the intracellular niche in order to proliferate and disseminate the *** invasion,*** resides temporarily in an acidic vacuole which is lysed by a not well-understood *** electron microscopy was used to describe the process of *** escape from the parasitophorous vacuole over the *** HeLa(non-professional phagocytic cells)as host cell,we observed that recently internalized parasites reside in a membrane-bounded vacuole.A few hours later,the first sign of vacuole disruption appeared as membrane *** observation was followed by a progressive vacuole swelling as evidenced by an electron-lucent halo between the parasite and the vacuole ***,the vacuole membrane remnants reorganized as small vesicles that eventually disappeared from the vicinity of the ***,parasites reach the host cell cytosol where replication takes *** thorough ultrastructural description of this process set the base for a comprehensive understanding of the parasite-host cell interaction and,thus open the possibility of new therapeutic intervention strategies.