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Cell bioenergetics:Simple logics,complex solut

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BIOCELL 2022年第10期46卷 2217-2220页

作者： LUIS S.MAYORGA Instituto de Histología y Embriología de Mendoza(ihem Universidad Nacional de CuyoCONICET)Facultad de Ciencias Exactas y NaturalesMendoza5500Argentina

Cells are open systems that exchange energy and molecules with their *** any material system,they perform all the complex activities required for homeostasis and reproduction,obeying the thermodynamic *** viewpoint will argue that the basic logic governing the energy flux required to preserve cell organization and function is simple:to decrease the activation energy(Ea)of specific *** none of the possible chemical reactions and energy transformations inside a cell occur at a measurable speed at room or body *** or other macromolecular structures speed up particular transformations by decreasing the corresponding energetic ***,to maintain the systems in a homeostatic state,capable of sophisticated functions based on this simple strategy requires an inconceivably complex *** conclusion will point to the challenging and intricate problems that cells have solved to carve the highly regulated channel through which the energy flows,fueling the work of these nanoscale machines.

关键词： Thermodynamics Cellular logics Self-organization Complexity

Coxiella burnetii:living inside the host cell

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BIOCELL 2019年第1期43卷 1-6页

作者： Jesus Sebastian DISTEL Walter BERON Rodolfo Matias ORTIZ FLORES Instituto de Histologia y Embriologia de Mendoza(ihem) ONICETUniversidad Nacional de CuyoMendozaArgentina Facultad de Odontologia Universidad Nacional de CuyoMendozaArgentina

Coxiella burnetii is an obligate intracellular pathogen and the causative agent of Q *** this brief review,we describe how recently described mechanisms help our understanding of *** invasion and its survival in the host cell by the formation of a replicative niche:the Coxiella-containing *** describe the actin-associated proteins involved in the internalization of ***,and we discuss the contribution of diverse degradation pathways of the cell during the formation and stabilization of the Coxiella-containing vacuole.

关键词： Actin Coxiellosis Phagocytic pathway Q fever

Tubulointerstitial injury and proximal tubule albumin transport in early diabetic nephropathy induced by type 1 diabetes mellitus

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BIOCELL 2017年第1期41卷 1-12页

作者： Maximiliano GIRAUD-BILLOUD Fernando EZQUER Javiera BAHAMONDE Marcelo EZQUER ihem Universidad Nacional de CuyoCONICETFacultad de Ciencias MédicasMendozaArgentina Centro de Medicina Regenerativa Facultad de Medicina Clínica AlemanaUniversidad del DesarrolloAvenida Las Condes 12438SantiagoChile

A decrease in the tubular expression of albumin endocytic transporters megalin and cubilin has been associated with diabetic nephropathy,but there are no comprehensive studies to date relating early tubulointerstitial injury and the effect of the disease on both transporters in type 1 diabetes mellitus(T1DM).We used eight-week-old male C57BL/6 mice divided into two groups;one of them received the vehicle(control group),while the other received the vehicle+200 mg/kg streptozotocin(T1DM).Ten weeks after the injection,we evaluated plasma insulin,enzymuria,urinary vitamin D-binding protein(VDBP),tubulointerstitial fibrosis and proximal tubule histology,markers of autophagy,and megalin and cubilin *** found a reduction in tubular protein reabsorption(albumin and VDBP as specific substances carried by both transporters)with increased tubulointerstitial injury,development of fibrosis,thickening of tubular basement membrane,and an increase in tubular cell *** was associated with a decrease in the renal expression of megalin and *** also observed an increase in the amount of cellular vesicles of the phagocytic system in the tubules,which could be linked to an alteration of normal intracellular trafficking of both receptors,thus affecting the normal function of transporters in early stages of diabetic *** diabetic animals,the added effects of tubulointerstitial injury,the decreases in megalin and cubilin expression,and an altered intracellular trafficking of these receptors,seriously affect protein reabsorption.

关键词： Albuminuria Megalin Cubilin Autophagy

Microglia-precursor cell interactions in health and in pathology

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BIOCELL 2018年第2期42卷 41-45页

作者： Estela M.MUÑOZ Instituto de Histología y Embriología de Mendoza Dr.Mario H.Burgos(ihem) Universidad Nacional de Cuyo(UNCuyo)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)MendozaArgentina

Until recently,microglia were mainly known as the resident phagocytes of the brain,***‘immunological warriors’of the ***,extensive knowledge is being accumulated about the functions of microglia beyond ***,it is well accepted that microglial cells are highly dynamic and responsive,and that they intervene in a dual manner in many developmental processes that shape the central nervous system,including neurogenesis,gliogenesis,spatial patterning,synaptic formation and elimination,and neural circuit establishment and *** differentiation and the pool of precursor cells were also shown to be under microglia regulation via bidirectional *** this concise review,I discuss our recent work in microglia-Pax6+cell interactions in one of the circumventricular organs,the pineal *** analogy with the rest of the central nervous system is also *** addition,I briefly examine mechanisms of interaction between microglia and non-microglial cells in both health and *** avenues are also introduced,which may lead us to better comprehend the impact of microglia in physiological and pathological conditions.

关键词： Phagocyte Phagocytosis Phagoptosis Apoptosis Autophagy

GTP-bound Rab3A exhibits consecutive positive and negative roles during human sperm dense-core granule exocytosis

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Journal of Molecular Cell Biology 2014年第4期8卷 286-298页

作者： Matias A. Bustos Carlos M. Roggero Paola X. De la Iglesia Luis S. Mayorga Claudia N. Tomes Instituto de Histologia y Embriologia IHEM-CONICET Facultad de Ciencias Medicas Universidad Nacional de Cuyo Mendoza Argentina Department of Biophysics UT Southwestern Medical Center Dallas TX USA Servicio de Patologla Hospital Italiano de Buenos Aires Buenos Aires Argentina

Exocytosis of mam maUan sperm dense-core secretory granule relies on the same fusion molecules as all other secretory cells; one such molecule is the small GTPase Rab3A. Here, we report an in-depth biochemical characterization of the role of Rab3A in secretion by scrutinizingthe exocytotic response of streptolysin O-permeabiUzed human sperm to the acute application of a number of Rab3A-containing constructs and correlating the findings with those gathered with the endogenous protein. Full length, geranyigeranyiated, and active Rab3A elicited human sperm exocytosis perse. With Rab3A/Rab22A chimeric proteins, we demonstrated that the carboxy-terminal domain of the Rab3A molecule was necessary and sufficient to promote exocytosis, whereas its *** prevented calcium-triggered secretion. Interestingly, full length Rab3A halted secretion when added after the docking of the acrosome to the plasma membrane. This effect depended on the inability of Rab3A to hydrolyze GTP. We combined modified immunofluorescence and acrosomal staining protocols to detect membrane fusion and the activation status of endogenous Rab3 simultaneously in individual cells, and found that GTP hydrolysis on endogenous Rab3 was mandatory for fusion pores to open. Our findings contribute to establishing that Rab3 modulates regulated exocytosis differently depending on the nucleotide bound and the exocytosis stage under study.

关键词： Rab3 cycle GTP hydrolysis exocytosis acrosome calcium sperm

Freely Available Tool(FAT)for automated quantification of lipid droplets in stained cells

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BIOCELL 2017年第2期41卷 55-58页

作者： DIEGO MASONE ALDANA DGOJANOVICH YESICA R.FRONTINI-LOPEZ SAMANTA DEL VELIZ MARINA UHART DIEGO M.BUSTOS Laboratorio de Integración de Senales Celulares.ihem-CONICET Universidad Nacional de Cuyo MendozaArgentina Facultad de Ingeniería Universidad Nacional de CuyoMendozaArgentina Facultad de Ciencias Exactas y Naturales Universidad Nacional de CuyoMendozaArgentina

In this study,we propose an automatic procedure for digital image *** describe a method that can efficiently quantify and characterize lipid droplets distributions in different cell types in ***,the lipid droplets detection method described in this work could be applied to static or time-lapse data,collected with a simple visible light or fluorescence microscopy *** automated algorithms were implemented in Octave,a freely available scientific package.

关键词： Adipogenesis 3T3-L1 cells human stem cells automatic image processing

The impact of paralog genes:detection of copy number variation in spinal muscle atrophy patients

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BIOCELL 2018年第3期42卷 87-91页

作者： Sergio LAURITO Juan A.CUETO Jimena PEREZ María ROQUé ihem Universidad Nacional de CuyoCONICET Departamento de Biología Universidad Nacional de CuyoFacultad de Ciencias Exactas y Naturales Instituto de Fisiología Universidad Nacional de CuyoFacultad de Ciencias Médicas

Spinal muscular atrophy(SMA)is caused by dysfunction of the alpha motor neurons of the spinal *** is an autosomal recessive disease associated to the SMN1 gene,located in the subtelomeric region of 5q13.A paralog SMN2 gene is located at the centromeric region of the same chromosome,which apparently originated by an ancestral inverted duplication occurring only in *** exon sequence differs in two nucleotides in exon 7 and exon 8,which leads to an SMN2 transcript that lacks exon 7 and results in a truncated ***(10%)of the SMN2 transcripts avoids the splicing of exon 7 but most of the copies are *** a disease scenario,the more SMN2 copies the higher possibility to restore at least partly the effects of SMN1 *** therapeutic approaches are being developed to increase the expression of *** determine the number of SMN1 and SMN2 copies,the methodology must distinguish accurately between both *** this work,we present the results obtained using multiplex ligation-dependent probe amplification(MLPA)in 60 SMA suspected patients/carriers derived from different regions of *** 32 of these DNA samples we found alterations in *** these,16 presented a heterozygous deletion(carrier status)and 14 an homozygous deletion(patient status)in exon 7 and 8 of *** one case,exon 7 was found homozygously deleted but exon 8 presented a single copy,and in another case,exon 7 was found heterozygously deleted while exon 8 was *** half of the patients(7/15)presented a normal diploid number of SMN2 while the other half(8/15)presented an increased *** this work we showed how a probe-based methodology such as MLPA was able to distinguish between the paralog genes and determine the amount of copies in DNA samples from suspected patients/carriers of SMA.

关键词： SMA CNV MLPA Paralog gene

Adipose-derived mesenchymal stem/stromal cells:from the lab bench to the basic concepts for clinical translation

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BIOCELL 2018年第3期42卷 67-77页

作者： Yesica Romina FRONTINI-LóPEZ Aldana Daniela GOJANOVICH Diego MASONE Diego Martín BUSTOS Marina UHART Laboratorio de Integración de Señles Celulares.ihem-CONICET Universidad Nacional de Cuyo MendozaArgentina Facultad de Ingeniería Universidad Nacional de CuyoMendozaArgentina Facultad de Ciencias Exactas y Naturales Universidad Nacional de CuyoMendozaArgentina

In the last years,much work has shown that the most effective repair system of the body is represented by stem cells,which are defined as undifferentiated precursors that own unlimited or prolonged self-renewal ability,which also have the potential to transform themselves into various cell types through *** tissues that form the body contain many different types of somatic cells,along with stem cells that are called‘mesenchymal stem(or stromal)cells’(MSC).In certain circumstances,some of these MSC migrate to injured tissues to replace dead cells or to undergo differentiation to repair *** discovery of MSC has been an important step in regenerative medicine because of their high ***,the finding of a method to isolate MSC from adipose tissue,so called‘adipose-derived mesenchymal stem cells’(ASC),which share similar differentiation capabilities and isolation yield that is greater than other MSC,and less bioethical concerns compared to embryonic stem cells,have created self-praised publicity to procure almost any treatment with ***,we review the current techniques for isolation,culture and differentiation of human ASC(hASC),and describe them in *** also compile some advantages of the hASC over other stem cells,and provide some concepts that could help finding strategies to promote their therapeutic efficiency.

关键词： hASC Tissue regeneration Cell therapy

Enhanced sampling for lipid-protein interactions during membrane dynamics

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BIOCELL 2023年第1期47卷 1-14页

作者： DIEGO MASONE Instituto de Histología y Embriología de Mendoza(ihem)-Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Universidad Nacional de Cuyo(UNCuyo)Mendoza5500Argentina Facultad de Ingeniería Universidad Nacional de Cuyo(UNCuyo)Mendoza5500Argentina

The inflexible concept of membrane curvature as an independent property of lipid structures is today *** bilayers behave as many-body entities with emergent properties that depend on their interactions with the *** particular,proteins exert crucial actions on lipid molecules that ultimately condition the collective properties of the *** this review,the potential of enhanced molecular dynamics to address cell-biology problems is *** cases of membrane deformation,membrane fusion,and the fusion pore are analyzed from the perspective of the dimensionality reduction by collective *** lipid-protein interactions as fundamental determinants of large membrane remodeling events are also ***,novel strategies merging cell biology and physics are considered as future lines of research.

关键词： Membrane bending Membrane fusion Fusion stalk Hemifusion diaphragm Fusion pore Molecular dynamics Collective variables Collective behavior Emergent properties