检索结果-南通市图书馆

Soil Bacterial Communities Under Different Long-Term Fertilization Regimes in Three Locations Across the Black Soil Region of Northeast china

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2018年第5期28卷 751-763页

作者： HU Xiaojing LIU Junjie WEI Dan ZHU Ping CUI Xi'an ZHOU Baoku CHEN Xueli JIN Jian LIU Xiaobing WANG Guanghua Key Laboratory of Mollisols Agroecology Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Harbin 150081 (China) University of Chinese Academy of sciences Beijing 100049 (China) Institute of Soil Fertilizer and Environment Resources Heilongjiang Academy of Agricultural Sciences Harbin 150086 (China) Institute of agricultural Resource and Environment Jilin Academy of Agricultural Sciences Changchun 130033 (China) Heihe Branch of Heilongjiang Academy of agricultural sciences Heihe 164300 (China)

Although soil bacteria play critical roles in agro-ecosystems, the knowledge of their response to long-term fertilization across the black soil region of Northeast china.is limited. In this study, we sequenced 16 S rRNA genes to assess the effects of four long-term fertilization regimes—non-fertilization(NoF), chemical fertilizer(CF), manure(M), and chemical fertilizer plus manure(CFM)—on soil properties and bacterial communities in three locations, the northern, middle, and southern parts, across the black soil region. Results showed that the influence of fertilization regimes on soil properties varied significantly among the three locations. Manure fertilization significantly increased microbial biomass carbon and relative abundance of copiotrophic bacteria. Principal component analysis(PCA)showed that the total bacterial communities were separated into three groups according to the sampling location despite long-term fertilization, and that soil pH was the most important factor in shifting bacterial communities. In addition, similar fertilization regimes resulted in different influences on bacterial community composition, and the most influential soil properties varied among the three locations. Our results highlighted that geographical separation was a more dominant factor affecting bacterial communities than fertilization, and that long-term similar fertilization regimes did not induce consistent changes in bacterial community composition in the black soil region.

关键词： chemical fertilizer geographical separation Illumina MiSeq sequencing Mollisols organic fertilizer soil properties 16SrRNA gene

Characterization of chromosome segment substitution lines reveals candidate genes associated with the nodule number in soybean

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第8期21卷 2197-2210页

作者： ZOU Jia-nan ZHANG Zhan-guo KANG Qing-lin YU Si-yang WANG Jie-qi CHEN Lin LIU Yan-ru MA Chao ZHU Rong-sheng ZHU Yong-xu DONG Xiao-hui JIANG Hong-wei WU Xiao-xia WANG Nan-nan HU Zhen-bang QI Zhao-ming LIU Chun-yan CHEN Qing-shan XIN Da-wei WANG Jin-hui College of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China Suihua Branch of Heilongjiang Academy of agricultural sciences Suihua 152000P.R.China Soybean Research Institute Jilin Academy of Agricultural SciencesChangchun 130033P.R.China Jiamusi Branch of Heilongjiang Academy of agricultural sciences Jiamusi 154000P.R.China

Soybean is one of the most important food crops *** other legumes,soybean can form symbiotic relationships with Rhizobium *** fixation of soybean via its symbiosis with Rhizobium is pivotal for sustainable *** Ⅲ effectors(T3Es)are essential regulators of the establishment of the symbiosis,and nodule number is a feature of nitrogen-affected ***,genes encoding T3Es at quantitative trait loci(QTLs)related to nodulation have rarely been *** segment substitution lines(CSSLs)have a common genetic background but only a few loci with heterogeneous genetic information;thus,they are suitable materials for identifying candidate genes at a target *** this study,a CSSL population was used to identify the QTLs related to nodule number in *** nucleotide polymorphism(SNP)markers and candidate genes within the QTLs interval were detected,and it was determined which genes showed differential expression between *** candidate genes(GmCDPK28,GmNAC1,GmbHLH,and GmERF5)linked to the SNPs were identified as being related to nodule traits and pivotal processes and pathways involved in symbiosis establishment.A candidate gene(GmERF5)encoding a transcription factor that may interact directly with the T3E NopAA was *** confirmed CSSLs with important segments and candidate genes identified in this study are valuable resources for further studies on the genetic network and T3Es involved in the signaling pathway that is essential for symbiosis establishment.

关键词： soybean TypeⅢeffectors nodule number chromosome segment substitution lines

Review of deep learning-based weed identification in crop fields

在线全文

学校读者我要写书评

暂无评论

International Journal of agricultural and Biological Engineering 2023年第4期16卷 1-10页

作者： Wenze Hu Samuel Oliver Wane Junke Zhu Dongsheng Li Qing Zhang Xiaoting Bie Yubin Lan School of agricultural Engineering and Food science Shandong University of TechnologyZibo 255000ShandongChina Department of agricultural Engineering Harper Adams UniversityNewport TF108NBShropshireUK School of Computer science and Technology Shandong University of TechnologyZibo 255000ShandongChina Academy of Ecological Unmanned Farm Zibo 255000ShandongChina

Automatic weed identification and detection are crucial for precision weeding *** recent years,deep learning(DL)has gained widespread attention for its potential in crop weed *** paper provides a review of the current research status and development trends of weed identification in crop fields based on *** an analysis of relevant literature from both within and outside of china.the author summarizes the development history,research progress,and identification and detection methods of DL-based weed identification *** is placed on data sources and DL models applied to different technical ***,the paper discusses the challenges of time-consuming and laborious dataset preparation,poor generality,unbalanced data categories,and low accuracy of field identification in DL for weed *** solutions are proposed to provide a reference for future research directions in weed identification.

关键词： deep learning weed detection weed classification cation image segmentation Convolutional Neural Network image processing

GRAIN INCOMPLETE FILLING 2 regulates grain filling and starch synthesis during rice caryopsis development

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2017年第2期59卷 134-153页

作者： Xiangjin Wei Guiai Jiao Haiyan Lin Zhonghua Sheng Gaoneng Shao Lihong Xie Shaoqing Tang Qingguo Xu Peisong Hu State Key Laboratory of Rice Biology Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture China National Rice Research Institute Hangzhou 310006 China . College of Agronomy Hunan Agricultural University Changsha 410128 China agricultural Genomes Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 China

Rice grain filling determines grain weight, final yield and grain quality. Here, a rice defective grain filling mutant, gif2, was identified. Grains ofgif2 showed a slower filling rate and a significant lower final grain weight and yield compared to wild-type. The starch content in gilt2 was noticeably decreased and its physicochemical properties were also altered. Moreover, gif2 endosperm cells showed obvious defects in compound granule formation. Posi- tional cloning identified GIF2 to encode an ADP-glucose pyrophosphorylase （AGP） large subunit, AGPL2; consequently, AGP enzyme activity in gif2 endosperms was remarkably decreased. GIF2 is mainly expressed in developing grains and the coded protein localizes in the cytosol. Yeast two hybrid assay showed that GIF2 interacted with AGP small subunits OsAGPS% OsAGPS2a and OsAGPS2b. Transcript levels for granule-bound starch synthase, starch synthase, starch branching enzyme and starch debranching enzyme were distinctly elevated in gif2 grains. In addition, the level of nucleotide diversity of the GIF2 locus was extremely low in both cultivated and wild rice. All of these results suggest that GIF2 plays important roles in the regulation of grain filling and starch biosynthesis during caryopsis development, and that it has been preserved during selection throughout domestication of modern rice.

关键词： GIF GRAIN INCOMPLETE FILLING 2 regulates grain filling and starch synthesis during rice caryopsis development Figure AGPS RNAi

Identification of propranolol and derivatives that are chemical inhibitors of phosphatidate phosphatase as potential broad-spectrum fungicides

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2024年第1期5卷 215-230页

作者： Juan Zhao Yu Chen Zhifen Ding Yaru Zhou Ruiqing Bi Ziting Qin Lei Yang Peng Sun Qiping Sun Guang Chen Daiyuan Sun Xianya Jiang Lu Zheng Xiao-Lin Chen Hu Wan Guanghui Wang Qiang Li Huailong Teng Guotian Li National Key Laboratory of agricultural Microbiology Hubei Hongshan LaboratoryHubei Key Laboratory of Plant PathologyThe Center of Crop NanobiotechnologyHuazhong Agricultural UniversityWuhan 430070China College of science Huazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYangling 712100China College of Chemistry and Life sciences Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological ResourcesChengdu Normal UniversityChengdu 611130China Yangjiang Institute of agricultural sciences Yangjiang 529500China

Plant diseases cause enormous economic losses in agriculture and threaten global food security,and application of agrochemicals is an important method of crop disease *** of disease-resis-tance mechanisms and synthesis of highly bioactive agrochemicals are thus important research ***,we show that propranolol,a phosphatidate phosphatase(Pah)inhibitor,effectively suppresses fungal growth,sporulation,sexual reproduction,and infection of diverse *** MoPah1 enzyme activity of the rice blast fungus Magnaporthe oryzae is inhibited by *** in lipid metabolism are associated with inhibited hyphal growth and appressorium formation caused by propranolol in *** inhibits a broad spectrum of 12 plant pathogens,effectively inhibiting infection of barley,wheat,maize,tomato,and *** improve antifungal capacity,we synthesized a series of propranolol derivatives,one of which shows a 16-fold increase in antifungal ability and binds directly to *** and its derivatives can also reduce the severity of rice blast and Fusarium head blight of wheat in *** together,our results demonstrate that propranolol suppresses fungal development and infection through mechanisms involved in lipid *** and its derivatives may therefore be promising candidates for fungicide development.

关键词： propranolol molecular design chemical modification virulence lipid metabolism Pyricularia oryzae

Electron-Ion Coupling Mechanism to Construct Stable Output Performance Nanogenerator

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research 2021年第1期2021卷 1123-1134页

作者： Yan-Yuan Ba Jing-Fu Bao Xin-Tian Liu Xiao-Wen Li Hai-Tao Deng Dan-liang Wen Xiao-Sheng Zhang School of Electronic science and Engineering University of Electronic Science and Technology of ChinaChengdu 611731China

Recently,triboelectric nanogenerators(TENGs)have been promoted as an effective technique for ambient energy harvesting,given their large power density and high energy conversion ***,traditional TENGs based on the combination of triboelectrification effect and electrostatic induction have proven susceptible to environmental influence,which intensively restricts their application ***,a new coupling mechanism based on electrostatic induction and ion conduction is proposed to construct flexible stable output performance TENGs(SOP-TENGs).The calcium chloride doped-cellulose nanofibril(CaCl_(2)-CNF)film made of natural carrots was successfully introduced to realize this coupling,resulting from its intrinsic properties as natural nanofibril hydrogel serving as both triboelectric layer and *** coupling of two conductive mechanisms of SOP-TENG was comprehensively investigated through electrical measurements,including the effects of moisture content,relative humidity,and electrode *** contrast to the conventional hydrogel ionotronic TENGs that require moisture as the carrier for ion transfer and use a hydrogel layer as the electrode,the use of a CaCl_(2)-CNF film(i.e.,ion-doped natural hydrogel layer)as a friction layer in the proposed SOP-TENG effectively realizes a superstable electrical output under varying moisture contents and relative humidity due to the compound transfer mechanism of ions and *** new working principle based on the coupling of electrostatic induction and ion conduction opens a wider range of applications for the hydrogel ionotronic TENGs,as the superstable electrical output enables them to be more widely applied in various complex environments to supply energy for low-power electronic devices.

关键词： moisture electrostatic coupling

A Furan-based Phosphaphenanthrene-containing Derivative as a Highly Efficient Flame-retardant Agent for Epoxy Thermosets without Deteriorating Thermomechanical Performances

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer science 2022年第3期40卷 233-240,I0005页

作者： Hao-Xin Niu Hong-Liang Ding Jia-Li Huang Xin Wang Lei Song Yuan Hu State Key Laboratory of Fire science University of Science and Technology of ChinaHefei 230026China

In order to reduce greenhouse gas emissions, developing flame retardants from bio-based resources has aroused extensive interest in recent years. In this work, we utilized furfural(biomass) and 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide(DOPO) to synthesize a biobased co-curing agent(FGD) to combine with 4,4'-diaminodiphenyl methane(DDM) for obtaining a low-phosphorus loading flame-retardant epoxy thermosets. The introduction of FGD decreased the activation energy of the curing progress, enhanced the mechanical properties of the epoxy thermosets, and did not affect the glass transition temperature of the epoxy thermosets. EP-5.0 had a lower thermal degradation rate and a doubled char yield compared with EP-0. The phosphorus content of EP-5.0 was only 0.45 wt%, while EP-5.0 reached the UL-94 V-0 rating with a high LOI value of 32%. Compared with EP-0, the PHRR of EP-2.5 and EP-5.0 decreased by 22.3% and 31.3%, respectively. The SEM results showed that the addition of FGD made the char residues more uniform and denser, which could effectively prevent combustible volatiles from escaping from the degradation area to the flame area and isolate the heat transfer so that the epoxy thermosets had an excellent flame-retardant performance.

关键词： Furfural DOPO Bio-based co-curing agent Flame-retardant performance

Two-dimensional electrophoresis for comparative proteomic analysis of human bile

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2007年第4期6卷 402-406页

作者： Bo Chen,Jing-Qing Dong,Yong-Jun Chen,Jian-Ming Wang,Jun Tian,Chun-Ben Wang and Sheng-Quan Zou Department of General Surgery,Tongji Hospital and Department of Proteomics Research Center,Tongji Medical College,Huazhong University of science and Technology,Wuhan 430030,china Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China.

BACKGROUND:Proteomic analysis of bile fluid holds promise as a method to identify biomarkers of bile tract diseases,especially for tumors. Two-dimensional electrophoresis(2-DE) is a popular and proven separation technique for proteome analysis,but using this strategy for bile fluid analysis is still not fully developed. This study was undertaken to(a) establish a reliable method for general clean-up to make bile fluid samples suitable for 2-DE;(b) obtain 2-D biliary maps with high reproducibility and resolution;and(c) identify protein patterns present in 2-D biliary maps for potential tumor biomarker discovery,with the intention of distinguishing malignant from benign causes of bile duct obstruction. METHODS:Bile fluid samples were obtained from two patients suffering from malignant and benign bile tract obstruction(one patient with cholangiocarcinoma as the experimental case,the other with cholelithiasis as control). A variety of sample preparation options,including delipidation,desalination and nucleic acid removal,were adopted to remove contaminants that affect 2-DE results. After that,each 350 μg purified sample was loaded onto nonlinear IPG strips(18 cm,pH 3-10 and pH 4-7) for first-dimension isoelectric focusing,and 12.5% SDS-PAGE electrophoresis for second dimension separation. Then 2-D maps were visualized after silver staining and analyzed with the Image Master 2-D software. RESULTS:A large number of protein spots were separated in 2-D maps from the experimental and control groups,with means of 250 and 216 spots on pH 3-10 IPG strips,and 182 and 176 spots on pH 4-7 strips,respectively. Approximately 16 and 23 spots were differentially expressed in matched pairs from the experimental and control cases using pH 3-10 and pH 4-7 strips. CONCLUSIONS:This study established a reliable sample preparation process suitable for 2-DE of bile fluid. By this method,2-D biliary maps with high reproducibility and resolution were obtained. The differentially displayed prote

关键词： proteome electrophoresis,gel,two-dimensional bile

Time-Dependent 2D Modeling of Magnetron Plasma Torch in Turbulent Flow

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Plasma science and Technology 2008年第3期10卷 328-335页

作者：黎林村夏维东 Department of Thermal science and Energy Engineering University of Science and Technology of China Hefei 230027 China

A theoretical model is presented to describe the electromagnetic, heat transfer and fluid flow phenomena within a magnetron plasma torch and in the resultant plume, by using a commercial computational fluid dynamics （CFD） code FLUENT. Specific calculations are pre- sented for a pure argon system （i.e., an argon plasma discharging into an argon environment）, operated in a turbulent mode. An important finding of this work is that the external axial magnetic field （AMF） may have a significant effect on the behavior of arc plasma and thus affects the resulting plume. The AMF impels the plasma to retract axially and expand radially. As a result, the plasma intensity distribution on the cross section of torch seems to be more uniform. Numerical results also show that with AMF, the highest plasma temperature decreases and the anode arc root moves upstream significantly, while the current density distribution at the anode is more concentrated with a higher peak value. In addition, the use of AMF then induces a strong backflow at the torch spout and its magnitude increases with the AMF strength but decreases with the inlet gas velocity.

关键词： plasma modeling axial magnetic field FLUENT