Soil Bacterial Communities Under Different Long-Term Fertilization Regimes in Three Locations Across the Black Soil Region of Northeast China

作     者：HU Xiaojing LIU Junjie WEI Dan ZHU Ping CUI Xi'an ZHOU Baoku CHEN Xueli JIN Jian LIU Xiaobing WANG Guanghua 

作者机构：Key Laboratory of Mollisols Agroecology Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Harbin 150081 (China) University of Chinese Academy of Sciences Beijing 100049 (China) Institute of Soil Fertilizer and Environment Resources Heilongjiang Academy of Agricultural Sciences Harbin 150086 (China) Institute of Agricultural Resource and Environment Jilin Academy of Agricultural Sciences Changchun 130033 (China) Heihe Branch of Heilongjiang Academy of Agricultural Sciences Heihe 164300 (China) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2018年第28卷第5期

页      面：751-763页

核心收录：

学科分类：07[理学] 0713[理学-生态学] 

基　　金：supported by the Strategic Priority Research Program of Chinese Academy of Sciences (No. XDB15010103) the Natural Science Foundation of China (No. 41771284) China Biodiversity Observation Networks (Sino BON) 

主　　题：chemical fertilizer geographical separation Illumina MiSeq sequencing Mollisols organic fertilizer soil properties 16SrRNA gene 

摘      要：Although soil bacteria play critical roles in agro-ecosystems, the knowledge of their response to long-term fertilization across the black soil region of Northeast China is limited. In this study, we sequenced 16 S rRNA genes to assess the effects of four long-term fertilization regimes—non-fertilization(NoF), chemical fertilizer(CF), manure(M), and chemical fertilizer plus manure(CFM)—on soil properties and bacterial communities in three locations, the northern, middle, and southern parts, across the black soil region. Results showed that the influence of fertilization regimes on soil properties varied significantly among the three locations. Manure fertilization significantly increased microbial biomass carbon and relative abundance of copiotrophic bacteria. Principal component analysis(PCA)showed that the total bacterial communities were separated into three groups according to the sampling location despite long-term fertilization, and that soil pH was the most important factor in shifting bacterial communities. In addition, similar fertilization regimes resulted in different influences on bacterial community composition, and the most influential soil properties varied among the three locations. Our results highlighted that geographical separation was a more dominant factor affecting bacterial communities than fertilization, and that long-term similar fertilization regimes did not induce consistent changes in bacterial community composition in the black soil region.