Characterization of chromosome segment substitution lines reveals candidate genes associated with the nodule number in soybean

作     者：ZOU Jia-nan ZHANG Zhan-guo KANG Qing-lin YU Si-yang WANG Jie-qi CHEN Lin LIU Yan-ru MA Chao ZHU Rong-sheng ZHU Yong-xu DONG Xiao-hui JIANG Hong-wei WU Xiao-xia WANG Nan-nan HU Zhen-bang QI Zhao-ming LIU Chun-yan CHEN Qing-shan XIN Da-wei WANG Jin-hui ZOU Jia-nan;ZHANG Zhan-guo;KANG Qing-lin;YU Si-yang;WANG Jie-qi;CHEN Lin;LIU Yan-ru;MA Chao;ZHU Rong-sheng;ZHU Yong-xu;DONG Xiao-hui;JIANG Hong-wei;WU Xiao-xia;WANG Nan-nan;HU Zhen-bang;QI Zhao-ming;LIU Chun-yan;CHEN Qing-shan;XIN Da-wei;WANG Jin-hui

作者机构：College of AgricultureNortheast Agricultural UniversityHarbin 150030P.R.China Suihua Branch of Heilongjiang Academy of Agricultural SciencesSuihua 152000P.R.China Soybean Research InstituteJilin Academy of Agricultural SciencesChangchun 130033P.R.China Jiamusi Branch of Heilongjiang Academy of Agricultural SciencesJiamusi 154000P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第8期

页      面：2197-2210页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：received from the National Natural Science Foundation of China(32070274,32072014 and 31971899) the China Postdoctoral Science Foundation(2020M681072) the Natural Science Foundation for the Excellent Youth Scholars of Heilongjiang Province,China(YQ2019C008) the Europe Horizon 2020(EUCLEG and 727312) the Youth Science and Technology Innovation Leader,China(2018RA2172) the National Key Research&Development Program of China(2016YFD0100500,2016YFD0100300 and 2016YFD0100201) the Heilongjiang Postdoctoral Science Foundation,China(LBH-Q16014) 

主　　题：soybean TypeⅢeffectors nodule number chromosome segment substitution lines 

摘      要：Soybean is one of the most important food crops *** other legumes,soybean can form symbiotic relationships with Rhizobium *** fixation of soybean via its symbiosis with Rhizobium is pivotal for sustainable *** Ⅲ effectors(T3Es)are essential regulators of the establishment of the symbiosis,and nodule number is a feature of nitrogen-affected ***,genes encoding T3Es at quantitative trait loci(QTLs)related to nodulation have rarely been *** segment substitution lines(CSSLs)have a common genetic background but only a few loci with heterogeneous genetic information;thus,they are suitable materials for identifying candidate genes at a target *** this study,a CSSL population was used to identify the QTLs related to nodule number in *** nucleotide polymorphism(SNP)markers and candidate genes within the QTLs interval were detected,and it was determined which genes showed differential expression between *** candidate genes(GmCDPK28,GmNAC1,GmbHLH,and GmERF5)linked to the SNPs were identified as being related to nodule traits and pivotal processes and pathways involved in symbiosis establishment.A candidate gene(GmERF5)encoding a transcription factor that may interact directly with the T3E NopAA was *** confirmed CSSLs with important segments and candidate genes identified in this study are valuable resources for further studies on the genetic network and T3Es involved in the signaling pathway that is essential for symbiosis establishment.