检索结果-南通市图书馆

Omicron spike protein:a clue for viral entry and immune evasion

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第10期7卷 3639-3640页

作者： G.M.N.Behrens A.Cossmann M.Hoffmann Department for Rheumatology and Immunology Hannover Medical SchoolHannoverGermany German Centre for infection Research partner site Hannover-BraunschweigHannoverGermany CiiM Centre for Individualized Infection MedicineHannoverGermany Infection Biology Unit German Primate CenterGöttingen 37077Germany Faculty of biology and Psychology Georg-August-University GöttingenGöttingenGermany

In a recent study in Science,Bowen et al.1 report that mutations in Omicron sublineage’s spike protein enhance ACE2 binding,impair fusogenicity,and dampen the neutralizing activity of antibodies after vaccination or infection.The data describe how Omicron and especially the BA.5 variant evade neutralizing antibody responses and argue for using mRNA booster vaccination to increase immunity.

关键词： ACE2 Bowen al.

Omicron:Master of immune evasion maintains robust ACE2 binding

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第5期7卷 1360-1362页

作者： Markus Hoffmann Lu Zhang Stefan Pohlmann Infection Biology Unit German Primate Center—Leibniz Institute for Primate ResearchGöttingenGermany Georg-August-University Göttingen GöttingenGermany

The spike(S)protein of the SARS-CoV-2 Omicron variant is highly mutated but the impact of these mutations on viral entry into cells and its inhibition by antibodies has been unclear.A recent study published in Science by Mannar et al.now shows that these mutations are compatible with robust ACE2 binding and allow for efficient evasion of neutralizing antibodies.The emergence of SARS-CoV-2 variants has become a hallmark of the COVID-19 pandemic.Variants of concern(VOC)harbor mutations in the viral S protein that can increase transmissibility,potentially by promoting S protein binding to the cellular receptor ACE2.

关键词： ACE2 maintain

Efficient antibody evasion but reduced ACE2 binding by the emerging SARS-CoV-2 variant B.1.640.2

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2022年第9期19卷 1067-1069页

作者： Prerna Arora Amy Kempf Inga Nehlmeier Luise Graichen Sebastian Schulz Anne Cossmann Alexandra Dopfer-Jablonka Martin S.Winkler Hans-Martin Jäck Georg M.N.Behrens Stefan Pöhlmann Markus Hoffmann Infection Biology Unit German Primate CenterKellnerweg 437077GöttingenGermany Faculty of biology and Psychology Georg-August-University GöttingenWilhelmsplatz 137073GöttingenGermany Division of Molecular Immunology Department of Internal Medicine 3Friedrich-Alexander University of Erlangen-NürnbergGlückstraße 691054ErlangenGermany Department for Rheumatology and Immunology Hannover Medical SchoolCarl-Neuberg-Straße 130625HannoverGermany German Centre for infection Research(DZIF) Partner Site Hannover-BraunschweigHannoverGermany Centre for Individualised infection Medicine(CiiM) HannoverGermany Department of Anesthesiology University of Göttingen Medical CenterGöttingenGeorg-August University of GöttingenRobert-Koch-Straße 4037075GöttingenGermany

The SARS-CoV-2 spike(S)protein engages ACE2 for cell entry,and the S protein/ACE2 interface is an important target for neutralizing antibodies.In the course of the COVID-19 pandemic,SARS-CoV-2 variants have emerged that harbor mutations in the S protein,which confer neutralization resistance and allow viral spread in immunologically nonnaive populations.The most prominent example is the highly mutated Omicron variant,which infects convalescent or vaccinated individuals with unprecedented efficiency[1,2].

关键词： ACE2 harbor neutral

Antibiotic Treatment Drives the Diversification of the Human Gut Resistome

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2019年第1期17卷 39-51页

作者： Jun Li Elizabeth A.Rettedal Eric van der Helm Mostafa Ellabaan Gianni Panagiotou Morten O.A.Sommer Department of Infectious Diseases and Public Health Colleague of Veterinary Medicine and Life Sciences City Univerity of Hong Kong School of Data Science City Univerity of Hong Kong Novo Nordisk Foundation Center for Biosustainability Systems biology and Bioinformatics unit Leibniz Institute for Natural Product Research and Infection Biology – Hans Kn?ll Institute Systems biology and Bioinformatics Group School of Biological Sciences Faculty of Sciences The University of Hong Kong Department of Microbiology Li Ka Shing Faculty of Medicine The University of Hong Kong

Despite the documented antibiotic-induced disruption of the gut microbiota, the impact of antibiotic intake on strain-level dynamics, evolution of resistance genes, and factors influencing resistance dissemination potential remains poorly understood. To address this gap we analyzed public metagenomic datasets from 24 antibiotic treated subjects and controls, combined with an in-depth prospective functional study with two subjects investigating the bacterial community dynamics based on cultivation-dependent and independent methods. We observed that shortterm antibiotic treatment shifted and diversified the resistome composition, increased the average copy number of antibiotic resistance genes, and altered the dominant strain genotypes in an individual-specific manner. More than 30% of the resistance genes underwent strong differentiation at the single nucleotide level during antibiotic treatment. We found that the increased potential for horizontal gene transfer, due to antibiotic administration, was ～3-fold stronger in the differentiated resistance genes than the non-differentiated ones. This study highlights how antibiotic treatment has individualized impacts on the resistome and strain level composition, and drives the adaptive evolution of the gut microbiota.

关键词： Antibiotics Resistome Gut microbiome Strain Evolution Horizontal gene transfer

New guidelines for fluorophore application in peroxisome targeting analyses in transient plant expression systems

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant biology 2019年第7期61卷 884-899页

作者： Christian Falter Nguyen Binh Anh Thu Saugat Pokhrel Sigrun Reumann Plant Biochemistry and infection biology Institute of Plant Science and Microbiology University of Hamburg

Peroxisome research has been revolutionized by proteome studies combined with in vivo subcellular targeting analyses. Yellow and cyan fluorescent protein(YFP and CFP) are the classical fluorophores of plant peroxisome research. In the new transient expression system of Arabidopsis seedlings co-cultivated with Agrobacterium we detected the YFP fusion of one candidate protein in peroxisomes, but only upon co-transformation with the peroxisome marker, CFP-PTS1. The data suggested that the YFP fusion was directed to peroxisomes due to its weak heterodimerization ability with CFP-PTS1,allowing piggy-back import into peroxisomes. Indeed, if co-expressed with monomeric Cerulean-PTS1(mCer-PTS1),the YFP fusion was no longer matrix localized. We systematically investigated the occurrence and extent of dimerization-based piggy-back import for different fluorophore combinations in five major transient plant expression systems. In Arabidopsis seedlings and tobacco leaves both untagged YFP and monomeric Venus were imported into peroxisomes if co-expressed with CFP-PTS1 but not with mCer-PTS1. By contrast, piggy-back import of cytosolic proteins was not observed in Arabidopsis and tobacco protoplasts or in onion epidermal cells for any fluorophore combination at any time point. Based on these important results we formulate new guidelines for fluorophore usage and experimental design to guarantee reliable identification of novel plant peroxisomal proteins.

关键词： transient plant expression systems fluorophore

Magnolol attenuates sepsis-induced gastrointestinal dysmotility in rats by modulating inflammatory mediators

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第48期14卷 7353-7360页

作者： Tie-Cheng Yang Shu-Wen Zhang Li-Na Sun Hong Wang Ai-Min Ren Intensive Care unit and Medical Department of infection Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of Pharmacology School of Chemical Biology and Pharmaceutical Sciences Capital Medical University Beijing 100069 China

AIM: To investigate the protective effects of magnolol on sepsis-induced inflammation and intestinal dysmotility. METHODS: Sepsis was induced by a single intraperitoneal injection of lipopolysaccharide (LPS). Male Wistar rats were randomly assigned to one of three treatment groups: magnolol prior to LPS injection (LPS/Mag group); vehicle prior to LPS injection (LPS/Veh group); vehicle prior to injection of saline (Control/Veh). Intestinal transit and circular muscle mechanical activity were assessed 12 h after LPS injection. Tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), monocyte chemoattractant protein-1 (MCP-1) and inducible nitric oxide synthase (iNOS) mRNA in rat ileum were studied by RT-PCR 2 h after LPS injection. Nuclear factor-κB (NF-κB) activity in the intestine was also investigated at this time using electrophoretic mobility shift assay. In addition, antioxidant activity was determined by measuring malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity in the intestine 2 h after LPS injection. RESULTS: Magnolol significantly increased intestinal transit and circular muscle mechanical activity in LPS- treated animals. TNF-α, MCP-1 and iNOS mRNA expression in the small intestine were significantly reduced after magnolol treatment in LPS-induced septic animals, compared with untreated septic animals. Additionally,magnolol significantly increased IL-10 mRNA expression in septic rat ileum. Magnolol also significantly suppressed NF-κB activity in septic rat intestine. In addition, magnolol significantly decreased MDA concentration and increased SOD activity in rat ileum. CONCLUSION: Magnolol prevents sepsis-induced suppression of intestinal motility in rats. The potential mechanism of this benefit of magnolol appears to be modulation of self-amplified inflammatory events and block of oxidative stress in the intestine.

关键词：脓血症运动性细胞活素类木兰醇脂多糖

Laboratory biosafety for handling emerging viruses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2017年第5期7卷 483-491页

作者： I.Made Artika Chairin Nisa Ma’roef Biosafety Level 3 unit Eijkman Institute for Molecular Biology Department of Biochemistry Faculty of Mathematics and Natural SciencesBogor Agricultural UniversityDarmaga Campus Emerging Virus Research unit Eijkman Institute for Molecular Biology

Emerging viruses are viruses whose occurrence has risen within the past twenty years,or whose presence is likely to increase in the near future.Diseases caused by emerging viruses are a major threat to global public health.In spite of greater awareness of safety and containment procedures,the handling of pathogenic viruses remains a likely source of infection,and mortality,among laboratory workers.There is a steady increase in both the number of laboratories and scientist handling emerging viruses for diagnostics and research.The potential for harm associated to work with these infectious agents can be minimized through the application of sound biosafety concepts and practices.The main factors to the prevention of laboratory-acquired infection are well-trained personnel who are knowledgable and biohazard aware,who are perceptive of the various ways of transmission,and who are professional in safe laboratory practice management.In addition,we should emphasize that appropriate facilities,practices and procedures are to be used by the laboratory workers for the handling of emerging viruses in a safe and secure manner.This review is aimed at providing researchers and laboratory personnel with basic biosafety principles to protect themselves from exposure to emerging viruses while working in the laboratory.This paper focuses on what emerging viruses are,why emerging viruses can cause laboratory-acquired infection,how to assess the risk of working with emerging viruses,and how laboratory-acquired infection can be prevented.Control measures used in the laboratory designed as such that they protect workers from emerging viruses and safeguard the public through the safe disposal of infectious wastes are also addressed.

关键词： Biosafety Emerging viruses Laboratory acquired infection Biorisk assessment Biorisk management

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

糖尿病与肠道菌群研究进展

Science Bulletin 2022年第17期67卷 1720-1723页

作者：贾伟平 Gianni Panagiotou Shanghai Key Laboratory of Diabetes Mellitus Department of Endocrinology and MetabolismShanghai Diabetes InstituteShanghai Clinical Center for DiabetesShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200233China Systems biology and Bioinformatics unit Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll InstituteJena 07745Germany State Key Laboratory of Pharmaceutical Biotechnology The University of Hong KongHong KongChina Department of Medicine The University of Hong KongHong KongChina

Diabetes mellitus is a global public health problem that causes great economic and social burdens.Its pathogenesis is complex and unclear.Recently,with considerable attention paid to the interaction between gut microbiota and host,gut microbiota has been found to play an important role in several chronic diseases,including diabetes.

关键词： diseases pathogenesis Diabetes

Plant virus-derived siRNAs“turn on”broad-spectrum plant immunity in wheat

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2021年第7期14卷 1038-1040页

作者： Paola Leonetti Vitantonio Pantaleo Department of biology Agricultural and Food SciencesInstitute for Sustainable Plant ProtectionBari UnitCNRBari 70126Italy

Viruses are the most abundant biological entities on Earth and the major carriers of disease and mortality as well as the drivers of global processes.How plant viruses potentially influence global processes is an emergent issue,particularly for their putative implications in applications.Upon infection and spread,plant viruses are capable of reprogramming host gene expression via RNA silencing by releasing viral suppressors of RNA silencing,viral small interfering RNAs(vsiRNAs),and by inducing the production of viral-associated small interfering RNAs(va-siRNAs).Recently,studies on various crop-virus pathosystems suggest that RNA silencing suppressors(RSSs)。

关键词： immunity mortality releasing