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RSPSSL:A novel high-fidelity Raman spectral preprocessing scheme to enhance biomedical applications and chemical resolution visualization

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Light(Science & Applications) 2024年第2期13卷 366-386页

作者： Jiaqi Hu Gina Jinna Chen Chenlong Xue Pei Liang Yanqun Xiang Chuanlun Zhang Xiaokeng Chi Guoying Liu Yanfang Ye Dongyu Cui De Zhang Xiaojun yu Hong Dang Wen Zhang Junfan Chen Quan Tang Penglai Guo Ho-Pui Ho Yuchao Li Longqing Cong Perry Ping Shum State key laboratory of Optical Fiber and Cable Manufacture Technology Guangdong Key Laboratory of Integrated Optoelectronics IntellisenseDepartment of EEESouthern University of Science and TechnologyShenzhen 518055China College of Optical and Electronic Technology China Jiliang UniversityHangzhou 310018China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapyGuangzhou 510060China Department of Ocean Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Department of Nephrology Chaozhou People’s HospitalChaozhou 521011China Clinical Research Design Division Sun Yatsen Memorial HospitalGuangzhouGuangdong 510120China School of Automation Northwestern Polytechnical UniversityXi’anShaanxi 710072China Department of biomedical Engineering The Chinese University of Hong KongHong KongChina guangdong Provincial key laboratory of Nanophotonic Manipulation Institute of NanophotonicsJinan UniversityGuangzhou 511443China

Raman spectroscopy has tremendous potential for material analysis with its molecular fingerprinting capability in many branches of science and *** is also an emerging omics technique for metabolic profiling to shape precision ***,precisely attributing vibration peaks coupled with specific environmental,instrumental,and specimen noise is *** Raman spectral preprocessing to remove statistical bias noise and sample-related errors should provide a powerful tool for valuable information ***,we propose a novel Raman spectral preprocessing scheme based on self-supervised learning(RSPSSL)with high capacity and spectral *** can preprocess arbitrary Raman spectra without further training at a speed of~1900 spectra per second without human *** experimental data preprocessing trial demonstrated its excellent capacity and signal fidelity with an 88%reduction in root mean square error and a 60%reduction in infinite norm(L__(∞))compared to established *** this advantage,it remarkably enhanced various biomedical applications with a 400%accuracy elevation(ΔAUC)in cancer diagnosis,an average 38%(few-shot)and 242%accuracy improvement in paraquat concentration prediction,and unsealed the chemical resolution of biomedical hyperspectral images,especially in the spectral fingerprint *** precisely preprocessed various Raman spectra from different spectroscopy devices,laboratories,and diverse *** scheme will enable biomedical mechanism screening with the label-free volumetric molecular imaging tool on organism and disease metabolomics profiling with a scenario of high throughput,cross-device,various analyte complexity,and diverse applications.

关键词： spectral scheme resolution

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Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis:Implications for bone health

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World Journal of Stem Cells 2024年第4期16卷 389-409页

作者： Ling-Ling Wang Zhan-Jin Lu Shun-Kui Luo Yun Li Zhe Yang Hong-Yun Lu Department of Gerontology The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Endocrinology and Metabolism The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina guangdong Provincial key laboratory of biomedical imaging The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Endocrinology and Metabolism Zhuhai People’s Hospital(Zhuhai Clinical Medical College of Jinan Universitythe First Hospital Affiliated with Medical College of Macao University of Science and Technology)Zhuhai 519000Guangdong ProvinceChina

BACKGROUND Osteoporosis(OP)has become a major public health problem *** OP treatments are based on the inhibition of bone resorption,and it is necessary to identify additional treatments aimed at enhancing *** the bone marrow(BM)niche,bone mesenchymal stem cells(BMSCs)are exposed to a hypoxic ***,a few studies have demonstrated that hypoxiainducible factor 2alpha(HIF-2α)is involved in BMSC osteogenic differentiation,but the molecular mechanism involved has not been *** To investigate the effect of HIF-2αon the osteogenic and adipogenic differentiation of BMSCs and the hematopoietic function of hematopoietic stem cells(HSCs)in the BM niche on the progression of *** Mice with BMSC-specific HIF-2αknockout(Prx1-Cre;Hif-2αfl/fl mice)were used for in vivo *** quantification was performed on mice of two genotypes with three interventions:Bilateral ovariectomy,semilethal irradiation,and dexamethasone ***,the hematopoietic function of HSCs in the BM niche was compared between the two mouse *** vitro,the HIF-2αagonist roxadustat and the HIF-2αinhibitor PT2399 were used to investigate the function of HIF-2αin BMSC osteogenic and adipogenic ***,we investigated the effect of HIF-2αon BMSCs via treatment with the mechanistic target of rapamycin(mTOR)agonist MHY1485 and the mTOR inhibitor *** The quantitative index determined by microcomputed tomography indicated that the femoral bone density of Prx1-Cre;Hif-2αfl/fl mice was lower than that of Hif-2αfl/fl mice under the three intervention *** vitro,Hif-2αfl/fl mouse BMSCs were cultured and treated with the HIF-2αagonist roxadustat,and after 7 d of BMSC adipogenic differentiation,the oil red O staining intensity and mRNA expression levels of adipogenesis-related genes in BMSCs treated with roxadustat were decreased;in addition,after 14 d of osteogenic differentiation,BMSCs treated with roxadusta

关键词： Hypoxia-inducible factor-2α Bone marrow niche Bone mesenchymal stem cells Osteoporosis Osteogenic/adipogenic differentiation Mechanistic target of rapamycin signaling pathway

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Neural-field-assisted transport-of-intensity phase microscopy: partially coherent quantitative phase imaging under unknown defocus distance

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Photonics Research 2024年第7期12卷 1494-1501页

作者： YANBO JIN LINPENG LU SHUN ZHOU JIE ZHOU YAO FAN CHAO ZUO Smart Computational imaging laboratory(SCILab) School of Electronic and Optical EngineeringNanjing University of Science and TechnologyNanjing 210094China Smart Computational imaging Research Institute(SCIRI)of Nanjing University of Science and Technology Nanjing 210019China Jiangsu key laboratory of Spectral imaging&Intelligent Sense Nanjing 210094China

The transport-of-intensity equation(TIE) enables quantitative phase imaging(QPI) under partially coherent illumination by measuring the through-focus intensities combined with a linearized inverse reconstruction algorithm. However, overcoming its sensitivity to imaging settings remains a challenging problem because of the difficulty in tuning the optical parameters of the imaging system accurately and because of the instability to long-time measurements. To address these limitations, we propose and experimentally validate a solution called neural-field-assisted transport-of-intensity phase microscopy(NFTPM) by introducing a tunable defocus parameter into neural field. Without weak object approximation, NFTPM incorporates the physical prior of partially coherent image formation to constrain the neural field and learns the continuous representation of phase object without the need for training. Simulation and experimental results of He La cells demonstrate that NFTPM can achieve accurate, partially coherent QPI under unknown defocus distances, providing new possibilities for extending applications in live cell biology.

关键词： field coherent phase

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In vivo label-free measurement of blood flow velocity symmetry based on dual line scanning third-harmonic generation microscopy excited at the 1700 nm window

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Journal of Innovative Optical Health Sciences 2024年第1期17卷 61-68页

作者： Hui Cheng Jincheng Zhong Ping Qiu Ke Wang key laboratory of Optoelectronic Devices and Systems of Ministry of Education and guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060 P.R.China

Measurement of bloodflow velocity is key to understanding physiology and pathology in *** most measurements are performed at the middle of the blood vessel,little research has been done on characterizing the instantaneous bloodflow velocity *** is mainly due to the lack of measurement technology with high spatial and temporal ***,we tackle this problem with our recently developed dual-wavelength line-scan third-harmonic generation(THG)imaging *** acquisition of dual-wavelength THG line-scanning signals enables measurement of bloodflow velocities at two radially symmetric positions in both venules and arterioles in mouse brain in *** results clearly show that the instantaneous bloodflow velocity is not symmetric under general conditions.

关键词： 1700 nm-Window third-harmonic generation imaging blood flow velocity

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Precise Planar-Twisted Molecular Engineering to Construct Semiconducting Polymers with Balanced Absorption and Quantum Yield for Efficient Phototheranostics

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Research 2024年第2期2023卷 227-238页

作者： Xiang Su Zhirong Bao Wei Xie Deliang Wang Ting Han Dong Wang Ben Zhong Tang Center for AIE Research Shenzhen Key Laboratory of Polymer Science and TechnologyGuangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and EngineeringShenzhen UniversityShenzhen 518060China School of biomedical and Pharmaceutical Sciences Guangdong University of TechnologyGuangzhou 510006China Department of Radiation and Medical Oncology Hubei Key Laboratory of Tumor Biological BehaviorsHubei Cancer Clinical Study CenterZhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Materials Chemistry Huzhou UniversityHuzhou 313000China School of Science and Engineering Shenzhen Institute of Aggregate Science and TechnologyThe Chinese University of Hong KongShenzhenGuangdong 518172China

Semiconducting polymers(SPs)have shown great feasibility as candidates for near-infrared-II(NIR-Ⅱ)fluorescence imaging-navigated photothermal therapy due to their strong light-harvesting ability and flexible ***,the fluorescence signal of traditional SPs tends to quench in their aggregate states owing to the strongπ-πstacking,which can lead to the radiative decay pathway shutting *** address this issue,aggregation-induced emission effect has been used as a rational tactic to boost the aggregate-state fluorescence of NIR-Ⅱ*** this contribution,we developed a precise molecular engineering tactic based on the block copolymerizations that integrate planar and twisted segments into one conjugated polymer backbone,providing great flexibility in tuning the photophysical properties and photothermal conversion capacity of *** monomers featured with twisted and planar architectures,respectively,were tactfully incorporated via a ternary copolymerization approach to produce a series of new *** optimal copolymer(SP2)synchronously shows desirable absorption ability and good NIR-Ⅱquantum yield on the premise of maintaining typical aggregation-induced emission characteristics,resulting in balanced NIR-Ⅱfluorescence brightness and photothermal ***-dispersible nanoparticles fabricated from the optimal SP2 show efficient photothermal therapeutic effects both in vitro and in *** in vivo investigation reveals the distinguished NIR-Ⅱfluorescence imaging performance of SP2 nanoparticles and their photothermal ablation toward tumor with prominent tumor accumulation ability and excellent biocompatibility.

关键词： property conducting twisted

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Exploring Novel Therapeutic Breakthroughs for Cancers:Potential Roles of miRNAs

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Proceedings of Anticancer Research 2024年第5期8卷 11-24页

作者： Muhammad Abubakar Rooma Ayyoub Baqaur Rehman Muhammad Naeem Kiani Usama Ahmed Jawaria Farooq Areeb Anwar Usmani Department of Biosciences COMSATS University IslamabadPark RdIslamabad Capital Territory 45550Pakistan guangdong key laboratory of biomedical measurements and ultrasound imaging School of Biomedical EngineeringShenzhen University Medical SchoolShenzhen UniversityShenzhen 518060China Department of Medicine School of Biomedical EngineeringShenzhen University Medical SchoolShenzhen 518060PR China Department of Zoology The Government Sadiq College Women University BahawalpurPakistan Research Coordinator Department of SurgeryAga Khan University and Hospital KarachiPakistan

MicroRNAs(miRNAs)are small non-coding RNAs,19-25 nucleotides in length,that regulate gene *** are involved in various cellular,biological,and pathological activities,including the development and progression of malignant *** studies have highlighted the significant roles of miRNA deregulation in various cancer types,involving critical pathways such as gene deletions and amplifications,abnormal epigenetic changes,and dysfunctional miRNA regulatory *** can function both as oncogenes and tumor suppressor genes in different *** cancer hallmarks are associated with deregulated miRNAs,including resistance to cell death,metastasis,sustained proliferative signaling,promotion of angiogenesis,and the suppression of growth *** research has demonstrated that miRNAs contribute to drug resistance in tumor cells by targeting genes linked to drug resistance or by influencing genes that regulate the cell cycle,apoptosis,and proliferation.A single miRNA often has tissue-specific regulatory functions and can affect multiple *** miRNA types have been identified as potential biomarkers for tumor diagnosis and critical targets for novel therapeutic *** review aims to explore the role of miRNAs in cancer progression,metastasis,and potential therapeutic interventions.

关键词： MicroRNAs Cancer Treatment

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Candidate reference measurement procedure for serum 17-hydroxyprogesterone quantification via isotope dilution liquid chromatography–tandem mass spectrometry

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iLABMED 2024年第2期2卷 98-107页

作者： Ji Zhang Yang Jiang Ting Liu Xiongwei Liu Hui Huang Yanxiang Cheng Jieyan Li Bangning Cheng Chungen Qian Xufu Xiang Jun-Fa Xu guangdong Provincial key laboratory of Medical Molecular Diagnostics Department of Clinical ImmunologyInstitute of Laboratory MedicineSchool of Medical TechnologyGuangdong Medical UniversityDongguanGuangdongChina Shenzhen YHLO Biotech Co. Ltd.ShenzhenGuangdongChina Shenzhen Longgang Central Hospital Clinical laboratory ShenzhenGuangdongChina The key laboratory for biomedical Photonics of MOE at Wuhan National laboratory for Optoelectronics-Hubei Bioinformatics and Molecular imaging key laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanHubeiChina

Background:Accurate quantification of 17-hydroxyprogesterone(17-OHP)in serum is vital for clinical and research ***,inter-laboratory variability in test results exists owing to the lack of a standardized reference measurement procedure(RMP).Therefore,in this study,we developed a highly accurate,cost-effective,and user-friendly candidate RMP(cRMP)for analyzing 17-OHP in ***:We quantified 17-OHP in serum using a one-step liquid–liquid extraction method with the addition of 17-OHP-^(13)C_(3),followed by liquid chromatography–tandem mass *** ability of these methods to suppress interference was evaluated by chromatographic *** assessed accuracy,specificity,the lower limit of quantitation,linearity,and matrix effects by following the standards specified by the Clinical and laboratory Standards *** consistency between the developed cRMP and the chemiluminescence method was evaluated through experiments with 120 clinical ***:The developed cRMP required only 8 min for accurate quantification of serum 17-OHP without bias from matrix effects or interference from 19 metabolites added as potential *** method exhibited favorable measurement performance,with a quantitation limit of 0.086 ng/mL,linear range of 0.1–400 ng/mL,a total imprecision of≤2.90%,spike recovery of 100.1%–100.6%,and relative deviations from assigned target values(RfB Institution)of−2.91%to 1.10%.The cRMP demonstrated good consistency with the conventional assay(chemiluminescence method),with a correlation coefficient R of ***:A cRMP with high accuracy,cost-effectiveness,and convenient operation was developed for quantifying 17-OHP in *** simplicity and robust performance make it an invaluable addition to the arsenal of analytical tools available for *** method can enhance the accuracy and reliability of 17-OHP measurements across various laboratories.

关键词： 17-hydroxyprogesterone isotope dilution liquid chromatography-tandem mass spectrometry reference measurement procedure serum

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Functional near-infrared spectroscopy in non-invasive neuromodulation

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Neural Regeneration Research 2024年第7期19卷 1517-1522页

作者： Congcong Huo Gongcheng Xu Hui Xie Tiandi Chen Guangjian Shao Jue Wang Wenhao Li Daifa Wang Zengyong Li Beijing Advanced Innovation Center for biomedical Engineering Key Laboratory for Biomechanics and Mechanobiology of Ministry of EducationSchool of Biological Science and Medical EngineeringBeihang UniversityBeijingChina Beijing key laboratory of Rehabilitation Technical Aids for Old-Age Disability National Research Center for Rehabilitation Technical AidsBeijingChina School of Mechatronic Engineering and Automation Foshan UniversityFoshanGuangdong ProvinceChina School of Rehabilitation Engineering Beijing College of Social AdministrationBeijingChina key laboratory of Neuro-functional Information and Rehabilitation Engineering of the Ministry of Civil Affairs BeijingChina

Non-invasive cerebral neuromodulation technologies are essential for the reorganization of cerebral neural networks,which have been widely applied in the field of central neurological diseases,such as stroke,Parkinson’s disease,and mental *** significant advances have been made in neuromodulation technologies,the identification of optimal neurostimulation paramete rs including the co rtical target,duration,and inhibition or excitation pattern is still limited due to the lack of guidance for neural *** r,the neural mechanism unde rlying neuromodulation for improved behavioral performance remains poorly ***,advancements in neuroimaging have provided insight into neuromodulation *** near-infrared spectroscopy,as a novel non-invasive optical brain imaging method,can detect brain activity by measuring cerebral hemodynamics with the advantages of portability,high motion tole rance,and anti-electromagnetic *** functional near-infra red spectroscopy with neuromodulation technologies offe rs an opportunity to monitor the cortical response,provide realtime feedbac k,and establish a closed-loop strategy integrating evaluation,feedbac k,and intervention for neurostimulation,which provides a theoretical basis for development of individualized precise neuro *** aimed to summarize the advantages of functional near-infra red spectroscopy and provide an ove rview of the current research on functional near-infrared spectroscopy in transcranial magnetic stimulation,transcranial electrical stimulation,neurofeedback,and braincomputer ***,the future perspectives and directions for the application of functional near-infrared spectroscopy in neuromodulation are *** conclusion,functional near-infrared spectroscopy combined with neuromodulation may promote the optimization of central pellral reorganization to achieve better functional recovery form central nervous system

关键词： brain-computer interface cerebral neural networks functional near-infrared spectroscopy neural circuit neurofeedback neurological diseases neuromodulation non-invasive brain stimulation transcranial electrical stimulation transcranial electrical stimulation

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scRNA-seq reveals that origin recognition complex subunit 6 regulates mouse spermatogonial cell proliferation and apoptosis via activation of Wnt/β-catenin signaling

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Asian Journal of Andrology 2024年第1期26卷 46-56页

作者： Shi-Wei Liu Jia-Qiang Luo Liang-Yu Zhao Ning-Jing Ou Chao-Yang Yu-Xiang Zhang Hao-Wei Bai Hong-Fang Sun Jian-Xiong Zhang Chen-Cheng Yao Peng Li Ru-Hui Tian Zheng Li Zi-Jue Zhu Department of Andrology Center for Men's HealthUrologic Medical CenterShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China State key Lab of Reproductive Medicine Nanjing Medical UniversityNanjing 211166China Shanghai key Lab of Reproductive Medicine Shanghai Jiao Tong University School of MedicineShanghai 200080China Department of Urology Guangdong Provincial Key Laboratory of Biomedical ImagingThe Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000China Department of Urology First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China

The regulation of spermatogonial proliferation and apoptosis is of great significance for maintaining *** single-cell RNA sequencing(scRNA-seq)analysis of the testis was performed to identify genes upregulated in *** scRNAseq analysis,we identified the spermatogonia upregulated gene origin recognition complex subunit 6(Orc6),which is involved in DNA replication and cell cycle regulation;its protein expression in the human and mouse testis was detected by western blot and *** explore the potential function of Orc6 in spermatogonia,the C18-4 cell line was transfected with control or Orc6 ***,5-ethynyl-2-deoxyuridine(EdU)and terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assays,flow cytometry,and western blot were used to evaluate its effects on proliferation and *** was revealed that ORC6 could promote proliferation and inhibit apoptosis of C18-4 *** RNA sequencing and bioinformatics analysis indicated that Orc6 was involved in the activation of wingless/integrated(Wnt)/β-catenin *** blot revealed that the expression ofβ-catenin protein and its phosphorylation(Ser675)were significantly decreased when silencing the expression of *** findings indicated that Orc6 was upregulated in spermatogonia,whereby it regulated proliferation and apoptosis by activating Wnt/β-catenin signaling.

关键词： cell proliferation ORCG scRNA-seq analysis spermatogonia Wnt/β-catenin signaling

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Interaction between microbubble and elastic microvessel in low frequency ultrasound field using finite element method

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Chinese Science Bulletin 2013年第3期58卷 291-298页

作者： SHEN YuanYuan WANG TianFu CHIN ChienTing DIAO XianFen CHEN SiPing guangdong key laboratory for biomedical measurements and ultrasound imaging National-Regional key Technology Engineering laboratory for Medical ultrasound

The interaction between microbubble and elastic microvessel wall has been hypothesized to be important in the mechanisms of therapeutic ultrasound *** this study,a 2D axisymmetric finite element numerical model is established to study the interaction between elastic microvessel wall and oscillating microbubble in low frequency ultrasound field using fluid solid interaction *** numerical results show that the bubble oscillation induces the vessel wall dilation and *** von Mises stress distribution over the microvessel wall is heterogeneous and the maximum value of the midpoint on the inner vessel wall could reach 0.23 MPa and 1.32 MPa under PNP 0.2 MPa and 0.5 MPa,*** the bubble collapses,the circumferential stress decreases rapidly and the transmural pressure increases ***,the circumferential stress becomes compressive with the maximum magnitude 1.83 MPa under PNP 0.5 MPa,larger than the maximum tension *** is possible that the rapid compression stress during bubble collapse plays important role in mechanical effect on microvessel wall endothelial lining disruption.

关键词：相互作用有限元法超声场微血管弹性微泡低频数值模型

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