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A Capra hircus chromosome 19 locus linked to milk production influences mammary conformation

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Journal of Animal Science and Biotechnology 2022年第3期13卷 697-706页

作者： Andrew Jiang Alex Ankersmit-Udy Sally-Anne Turner Megan Scholtens Mathew D.Littlejohn Nicolas Lopez-Villalobos Colin G.Proser Russell G.Snell Klaus Lehnert Applied Translational Genetics Group School of Biological SciencesThe University of AucklandAucklandNew Zealand Dairy goat Co-operative HamiltonNew Zealand Cawthron Institute NelsonNew Zealand AL Rae Centre of Genetics and breeding Massey UniversityHamiltonNew Zealand Dairy Cattle breeding and Genetics School of Agriculture&EnvironmentMassey UniversityHamiltonNew Zealand

Background:Economically important milk production traits including milk volume,milk fat and protein yield vary considerably across dairy goats in New Zealand.A significant portion of the variation is attributable to genetic *** of genetic markers linked to milk production traits can be utilised to drive selection of highperformance animals.A previously reported genome wide association study across dairy goats in New Zealand identified a quantitative trait locus(QTL)located on chromosome *** most significantly associated single nucleotide polymorphism(SNP)marker for this locus is located at position 26,610,610(SNP marker rs268292132).This locus is associated with multiple milk production traits including fat,protein and *** predicted effect of selection for the beneficial haplotype would result in an average production increase of 2.2 kg fat,1.9 kg protein and 73.6 kg milk *** outstanding question was whether selection for the beneficial allele would co-select for any negative pleiotropic *** adverse relationship between milk production and udder health traits has been reported at this ***,a genome wide association study was undertaken looking for loci associated with udder ***:The QTL and production associated marker rs268292132 was identified in this study to also be associated with several goat udder traits including udder depth(UD),fore udder attachment(FUA)and rear udder attachment(RUA).Our study replicates the negative relationship between production and udder traits with the high production allele at position 19:26,610,610(SNP marker rs268292132)associated with an adverse change in UD,FUA and ***:Our study has confirmed the negative relationship between udder traits and production traits in the NZ goat *** have found that the frequency of the high production allele is relatively high in the NZ goat population,indicating that its effect on udder conformation is not significantly

关键词： Capra hircus Milk production Pleiotropic effects Quantitaive trait loci Udder conformation

Plasma and cerebrospinal fluid interleukin-1β during lipopolysaccharide-induced systemic inflammation in ewes implanted or not with slow-release melatonin

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Journal of Animal Science and Biotechnology 2018年第1期9卷 119-126页

作者： Janina Skipor Marta Kowalewska Aleksandra Szczepkowska Anna Majewska Tomasz Misztal Marek Jalynski Andrzej P.Herman Katarzyna Zabek Institute of Animal Reproduction and Food Research Polish Academy of Sciences The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences Veterinary Medicine Faculty University of Warmia and Mazury Department of Sheep and goat breeding Animal Bioengineering FacultyUniversity of Warmia and Mazury in Olsztyn

Background： Interleukin-1β（IL-1β） is important mediator of inflammatory-induced suppression of reproductive axis at the hypothalamic level. At the beginning of inflammation, the main source of cytokines in the cerebrospinal fluid（CSF） is peripheral circulation, while over time, cytokines produced in the brain are more important. Melatonin has been shown to decrease pro-inflammatory cytokines concentration in the brain. In ewes, melatonin is used to advance the onset of a breading season. Little is known about CSF concentration of IL-1β in ewes and its correlation with plasma during inflammation as well as melatonin action on the concentration of IL-1β in blood plasma and the CSF, and brain barriers permeability in early stage of lipopolysaccharide（LPS）-induced ***： Systemic inflammation was induced through LPS administration in melatonin-and sham-implanted ewes. Blood and CSF samples were collected before and after LPS administration and IL-1β and albumin concentration were measured. To assess the functions of brain barriers albumin quotient（QAlb） was *** of IL-1β（Il1B） and its receptor type Ⅰ（Il1r1） and type Ⅱ（Il1r2） and matrix metalloproteinase（Mmp） 3 and 9 was evaluated in the choroid plexus（CP）.Results： Before LPS administration, IL-1β was on the level of 62.0 ± 29.7 pg/mL and 66.4 ± 32.1 pg/mL in plasma and 26.2 ± 5.4 pg/mL and 21.3 ± 8.7 pg/mL in the CSF in sham-and melatonin-implanted group, *** LPS it increased to 159.3 ± 53.1 pg/mL and 197.8 ± 42.8 pg/mL in plasma and 129.8 ± 54.2 pg/mL and139.6 ± 51.5 pg/mL in the CSF. No correlations was found between plasma and CSF IL-1β concentration after LPS in both groups. The QAlb calculated before LPS and 6 h after was similar in all groups. Melatonin did not affected m RNA expression of Il1B, Il1r1 and Il1r2 in the CP. The m RNA expression of Mmp3 and Mmp9 was not ***： The lack of correlation between plasma and CSF IL-1β concentration indicates that

关键词： Albumin Cerebrospinal fluid Ewes Interleukin-1β LPS Melatonin

Whole-genome methylation analysis reveals epigenetic variation between wild-type and nontransgenic cloned,ASMT transgenic cloned dairy goats generated by the somatic cell nuclear transfer

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Journal of Animal Science and Biotechnology 2023年第1期14卷 98-113页

作者： Hao Wu Wendi Zhou Haijun Liu Xudai Cui Wenkui Ma Haixin Wu Guangdong Li Likai Wang Jinlong Zhang Xiaosheng Zhang Pengyun Ji Zhengxing Lian Guoshi Liu National Engineering Laboratory for Animal breeding Key Laboratory of Animal Genetics and Breeding of the Ministry of AgriculturalBeijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China. Sany Institute of China Agricultural University Sanya 572025China. Institute of Animal Husbandry and Veterinary Academy of Agricultural Sciences of TianjinTianjin 300192China. Qingdao Senmiao Industrial Co. Ltd.Qingdao 266101China.

Background:SCNT(somatic cell nuclear transfer)is of great significance to biological research and also to the livestock ***,the survival rate of the SCNT cloned animals is relatively low compared to other transgenic *** indicates the potential epigenetic variations between *** methylation is a key marker of mammalian epigenetics and its alterations will lead to phenotypic *** this study,ASMT(acetylserotonin-Omethyltransferase)ovarian overexpression transgenic goat was produced by using *** investigate whether there are epigenetic differences between cloned and WT(wild type)goats,WGBS(whole-genome bisulfite sequencing)was used to measure the whole-genome methylation of these ***:It is observed that the different m Cp G sites are mainly present in the intergenic and intronic regions between cloned and WT animals,and their CG-type methylation sites are strongly ***(differentially methylated region)lengths are located around 1000 bp,mainly distributed in the exonic,intergenic and intronic functional domains.A total of 56 and 36 DMGs(differentially methylated genes)were identified by GO and KEGG databases,*** annotation showed that DMGs were enriched in biological-process,cellularcomponent,molecular-function and other signaling pathways.A total of 10 identical genes related to growth and development were identified in GO and KEGG ***:The differences in methylation genes among the tested animals have been identified.A total of 10 DMGs associated with growth and development were identified between cloned and WT *** results indicate that the differential patterns of DNA methylation between the cloned and WT goats are probably caused by the *** novel observations will help us to further identify the unveiled mechanisms of somatic cell cloning technology,particularly in goats.

关键词： Acetylserotonin-O-methyltransferase Dairy goat DNA methylation Gene editing Somatic cell nuclear transfer

Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第11期22卷 893-905页

作者： Zhinan ZHOU Xiang CHEN Min ZHU Weiwei WANG Zheng AO Jiafu ZHAO Wen TANG Lei HONG Key Laboratory of Plateau Mountain Animal Genetics Breeding and Reproduction of Ministry of EducationKey Laboratory of Animal GeneticsBreeding and Reproduction of Guizhou ProvinceCollege of Animal ScienceGuizhou UniversityGuiyang 550025China

Cathepsin D(CTSD),the major lysosomal aspartic protease that is widely expressed in different tissues,potentially regulates the biological behaviors of various *** granulosa cells are responsive to the increase of ovulation number,hence indirectly influencing litter ***,the mechanism underlying the effect of CTSD on the behaviors of goat granulosa cells has not been fully *** study used immunohistochemistry to analyze CTSD localization in goat ovarian ***,western blotting was applied to examine the differential expression of CTSD in the ovarian tissues of monotocous and polytocous ***,the effects of CTSD knockdown on cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of the prolific traits,including bone morphogenetic protein receptor IB(BMPR-IB),follicle-stimulating hormone(FSHR),and inhibinα(INHA),were determined in granulosa *** showed that CTSD was expressed in corpus luteum,follicle,and granulosa ***,CTSD expression in the monotocous group was significantly higher than that in the polytocous *** addition,CTSD knockdown could improve granulosa cell proliferation,inhibit cell apoptosis,and significantly elevate the expression of proliferating cell nuclear antigen(PCNA)and B cell lymphoma 2(Bcl-2),but it lowered the expression of Bcl-2-associated X(Bax)and ***,CTSD knockdown significantly reduced the ratios of cells in the G0/G1 and G2/M phases but substantially increased the ratio of cells in the S *** expression levels of cyclin D2 and cyclin E were elevated followed by the obvious decline of cyclin A1 ***,the expression levels of BMPR-IB,FSHR,and INHA clearly increased as a result of CTSD ***,our findings demonstrate that CTSD is an important factor affecting the litter size trait in goats by regulating the granulosa cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of th

关键词： Cathepsin D(CTSD) Litter size trait Granulosa cells Cell apoptosis Cell cycle Cell proliferation

Effect of exogenous progesterone on cumulus characteristics of buffalo oocytes by allowing passage of more number of sperm through cumulus but not essentially fertilization

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Asian pacific Journal of Reproduction 2018年第2期7卷 79-86页

作者： Madhusmita Panda Asmita Sandeep Kumar Purusottam Mishra Mahesh Chandra Sahu Sachinandan De Tirtha Kumar Datta Rakesh Kumar Nodal Officer Chief District Veterinary OfficeKadambedaBhadrak756100OdishaIndia Institute of Microbial Technology Council of Scientific and Industrial ResearchChandigarhIndia BSL-4 National Institute of VirologySus RoadPashanPune-411021India goat breeding farm AT/PO-SalapadaDistrict-KeonjharPin code-758020State-OdishaIndia Directorate of Medical Research IMS and SUM HospitalSiksha'O'Anusandhan UniversityK8Kalinga NagarBhubaneswar-751003OdishaIndia Animal Biotechnology Centre National Dairy Research InstituteKarnal132001HaryanaIndia

Objective:To understand the level of progesterone (P4) in different quality of buffalo cumulus oocyte complexes (COCs) and further to evaluate the effect of exogenous P4 supplementation on maturation and subsequent developmental ability of poor quality brilliant cresyl blue (BCB-)***: Progesterone secreted by different quality of buffalo oocytes was estimated by enzyme linked immunosorbent assay and the concentration differences were translated into P4 doses to be incorporated in the maturation medium of BCB-ve COCs followed by expression analysis of genes involved in the cumulus expansion, extracellular matrix disintegration and progesterone receptor signalling. In addition, the study also evaluated the effect of exogenous P4 on sperm-cumulus interaction. Results: More than 10-fold up-regulated expression of progesterone receptor in P4 supplemented oocytes signified that P4 might be acting predominantly through this receptor. Also, exogenous P4 supplementation had significant effect on transcatheter arterial chemoembolization protease regulated by P4-progesterone receptor pathway which in turn had an important role in extracellular matrix disintegration. On the contrary, cumulus expansion genesHAS2,TNFAIP6,AREG were not altered upon P4 supplementation. Also, it was observed that P4 addition did facilitate passage of significantly more number of spermatozoa through P4 treated cumulus cells. Further, incorporation of different doses of P4 did not improve significantly the cleavage and blastocyst rates of BCB-ve ***: Different qualities of buffalo COCs secrete substantially diverse levels of P4, and its supplementation has a role in oocyte maturation via modulation of cumulus characteristics but perhaps not fertilization.

关键词： BCB screening Progesterone Sperm cumulus interaction Fertilizing ability

A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats

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Zoological Research 2023年第2期44卷 303-314页

作者： Ying Yuan Wei-Yi Zhang Bai-Gao Yang Dong-Ke Zhou Lu Xu Yong-Meng He Hao-Yuan Zhang Cheng-Li Liu Yue-Hui Ma Ming-Xing Chu Wen-Guang Zhang Hui-Jiang Gao Lin Jiang Fu-Ping Zhao Lu-Pei Zhang Ri-Su Na Baatarchogt Oyungerel Yan-Guo Han Yan Zeng Shi-Zhi Wang Huai-Zhi Jiang Hong-Ping Zhang Xun-Ping Jiang Jian-Ning He Hao Liang Kaushalendra Kaushalendra Ya-Wang Sun Yong-Fu Huang Yong-Ju Zhao Zhong-Quan Zhao Guang-Xin E College of Animal Science and Technology Southwest UniversityChongqing 400715China Chongqing Key Laboratory of Forage&Herbivore Chongqing 400715China Institute of Animal Science Chinese Academy of Agricultural Sciences(CAAS)Beijing 100193China College of Animal Science Inner Mongolia Agricultural UniversityHuhhotInner Mongolia 010018China School of Animal Science&Biotechnology Mongolian University of Life SciencesZaisanKhan-uulUlaanbaatar 17024Mongolia Animal Science and Technology College Jilin Agriculture UniversityChangchunJilin 130118China farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province College of Animal Science and TechnologySichuan Agricultural UniversityChengduSichuan 611130China Key Lab of Agricultural Animal GeneticsBreeding and Reproduction of Ministry of EducationCollege of Animal Science and TechnologyHuazhongAgricultural UniversityWuhanHubei 430070China College of Animal Science and Technology Qingdao Agricultural UniversityQingdaoShandong 266109China State Key Laboratory of Reproductive Regulation and breeding of Grassland Livestock School of Life SciencesInner Mongolia UniversityHohhotInner Mongolia 010070China Department of Zoology Pachhunga University CollegeMizoram UniversityAizawl 796001India

The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth ***,the genetic basis of muscle development in the Boer goat remains *** this study,we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development.A total of9 959 autosomal copy number variations(CNVs) were identified through selection signal analysis in 127 goat ***,we confirmed that the highest signal CNV(HSV) was a chromosomal arrangement containing an approximately 1.11 Mb(CHIR17:60062304-61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region(CHIR17:60145940-60151302 bp) with overlapping genes(e.g.,ARHGAP10,NR3C2,EDNRA,PRMT9,and TMEM184C).The homozygous duplicated HSV genotype(+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African *** expression network of three candidate genes(ARHGAP10,NR3C2,and EDNRA)regulating dose transcription was constructed by RNA *** indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells(SMSCs) and their overexpression significantly increased the expression of *** HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.

关键词： Boer goat CNV Muscle development SMSCs

TLR7/8 signalling affects X-sperm motility via the GSK3α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

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Journal of Animal Science and Biotechnology 2022年第2期13卷 401-417页

作者： Fa Ren Huaming Xi Yijie Ren Yu Li Fei Wen Ming Xian Mengjie Zhao Dawei Zhu Liqiang Wang Anmin Lei Jianhong Hu Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityNo.22 Xinong RoadYanglingShaanxi 712100People’s Republic of China College of Veterinary Medicine Northwest A&F UniversityYangling 712100ShaanxiChina

Background:goat milk is very similar to human milk in terms of its abundant nutrients and ease of *** derive greater economic benefit,farmers require more female offspring(does);however,the buck-to-doe offspring sex ratio is approximately 50%.At present,artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock ***,flow cytometry has not been successfully utilised for the separation of X/Y sperm aimed at sexing control in dairy ***:In this study,a novel,simple goat sperm sexing technology that activates the toll-like receptor 7/8(TLR7/8),thereby inhibiting X-sperm motility,was *** results showed that the TLR7/8 coding goat Xchromosome was expressed in approximately 50%of round spermatids in the testis and sperm,as measured from cross-sections of the epididymis and ejaculate,***,TLR7/8 was located at the tail of the *** TLR7/8 activation,phosphorylated forms of glycogen synthase kinaseα/β(GSK3α/β)and nuclear factor kappa-B(NF-κB)were detected in the X-sperm,causing reduced mitochondrial activity,ATP levels,and sperm ***-motility Y-sperm segregated to the upper layer and the low-motility X-sperm,to the lower *** in vitro fertilisation using the TLR7/8-activated sperm from the lower layer,80.52±6.75%of the embryos were XX *** TLR7/8-activated sperm were subsequently used for in vivo embryo production via the superovulatory response;nine embryos were collected from the uterus of two does that *** of these were XX embryos,and one was an XY ***:Our study reveals a novel TLR7/8 signalling mechanism that affects X-sperm motility via the GSK3α/β-hexokinase pathway;this technique could be used to facilitate the efficient production of sexed dairy goat embryos.

关键词： Dairy goat Glycogen synthase kinaseα/β(GSK3α/β) Sexing control Sperm Toll-like receptor 7/8(TLR7/8)

Changes in histology,protein expression,and autophagy in dairy goat testes during non-breeding season

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Changes in histology,protein expression,and autophagy in dai...

Gut microbiota-derived metabolites contribute negatively to hindgut barrier function development at the early weaning goat model

中国畜牧兽医学会动物繁殖学分会第二十次学术研讨会

作者：席华明 Fa Ren Yu Li Ming Xian 胡建宏 Key Laboratory of Animal Genetics breeding and Reproduction of Shaanxi Province College of Animal Science and Technology Northwest A&F University

Introduction Reproduction is the process by which organisms produce new *** growth,reproduction,and energy metabolism of most mammals are affected by *** reproduction is a self-protection measure used by animals to ad...

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Animal Nutrition 2022年第3期10卷 111-123页

作者： Ke Zhang Yangbin Xu Yuxin Yang Mengmeng Guo Ting Zhang Bo Zong Shuhong Huang Langda Suo Baohua Ma Xiaolong Wang Yujiang Wu Daniel Brugger Yulin Chen Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling712100China College of Veterinary Medicine Northwest A&F UniversityYangling712100China Institute of Animal Sciences Tibet Academy of Agricultural and Animal Husbandry SciencesLhasa850009China Institute of Animal Nutrition and Dietetics Vetsuisse-FacultyUniversity of Zurich8057ZurichSwitzerland

Early weaning induces intestinal injury,leading to a series of long-term symptoms such as inflammation,malabsorption and *** this study,we hypothesized that microbes and theirmetabolitesmodulate the host's inflammatory response to early weaning stress in a goatmodel.A total of 18 female Tibetan goat kids(n?9)wereweaned fromtheirmothers at 28 d(D28)and 60 d(D60)postpartum.D60 and D28 groupswere fed the same solid diet ad libitum fromweaning to 75 d of *** colonic epithelium was subject to RNAsequencing,the caecal digesta metabolomics were assessed by liquid chromatographyetandem mass spectrometry(LC-MS/MS),and the caecal microbiota composition was analysed by 16S ribosomal RNA gene *** foundthatearlyweaningsubstantially increased the colonic pro-apoptotic gene expressionof B-cell lymphoma associated X(Bax),caspase-9,and caspase-3,and decreased the expression of zonula occludens-1(ZO-1)and claudin-1(Pgoats(Pgoats to defaunated C57/6J mice,we confirmed that caecalmicrobiota of D28 goat kids increased the relative abundance of *** and significantly upregulated the genes of Bax,G proteinecoupled receptor(GPR)109A,GPR 43,fatty acid binding protein 6,nuclear receptor subfamily 1 group H member 3,angiotensin converting enzyme 2,and IL-6 expression(P<0.05),and decreased ZO-1,and claudin-1 protein expression in the mice jejunum and colon(P<0.001).These results proposed that the hindgut microbiota andmetabolites mediate the barrier functionweakening duringearlyweaning,and the relative abundance of *** negatively corr

关键词： Hindgut microbiome Ruminant Dysbiosis Inflammation Colon