Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats

作     者：Zhinan ZHOU Xiang CHEN Min ZHU Weiwei WANG Zheng AO Jiafu ZHAO Wen TANG Lei HONG Zhinan ZHOU;Xiang CHEN;Min ZHU;Weiwei WANG;Zheng AO;Jiafu ZHAO;Wen TANG;Lei HONG

作者机构：Key Laboratory of Plateau Mountain Animal GeneticsBreeding and Reproduction of Ministry of EducationKey Laboratory of Animal GeneticsBreeding and Reproduction of Guizhou ProvinceCollege of Animal ScienceGuizhou UniversityGuiyang 550025China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2021年第22卷第11期

页      面：893-905页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：the National Key R&D Program of China(No.2017YFD0501904) the National Natural Science Foundation of China(No.31760652) the Major Projects of Science and Technology in Guizhou Province(QK-major projects 2016-3002),China 

主　　题：Cathepsin D(CTSD) Litter size trait Granulosa cells Cell apoptosis Cell cycle Cell proliferation 

摘      要：Cathepsin D(CTSD),the major lysosomal aspartic protease that is widely expressed in different tissues,potentially regulates the biological behaviors of various *** granulosa cells are responsive to the increase of ovulation number,hence indirectly influencing litter ***,the mechanism underlying the effect of CTSD on the behaviors of goat granulosa cells has not been fully *** study used immunohistochemistry to analyze CTSD localization in goat ovarian ***,western blotting was applied to examine the differential expression of CTSD in the ovarian tissues of monotocous and polytocous ***,the effects of CTSD knockdown on cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of the prolific traits,including bone morphogenetic protein receptor IB(BMPR-IB),follicle-stimulating hormone(FSHR),and inhibinα(INHA),were determined in granulosa *** showed that CTSD was expressed in corpus luteum,follicle,and granulosa ***,CTSD expression in the monotocous group was significantly higher than that in the polytocous *** addition,CTSD knockdown could improve granulosa cell proliferation,inhibit cell apoptosis,and significantly elevate the expression of proliferating cell nuclear antigen(PCNA)and B cell lymphoma 2(Bcl-2),but it lowered the expression of Bcl-2-associated X(Bax)and ***,CTSD knockdown significantly reduced the ratios of cells in the G0/G1 and G2/M phases but substantially increased the ratio of cells in the S *** expression levels of cyclin D2 and cyclin E were elevated followed by the obvious decline of cyclin A1 ***,the expression levels of BMPR-IB,FSHR,and INHA clearly increased as a result of CTSD ***,our findings demonstrate that CTSD is an important factor affecting the litter size trait in goats by regulating the granulosa cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of th