Effect of exogenous progesterone on cumulus characteristics of buffalo oocytes by allowing passage of more number of sperm through cumulus but not essentially fertilization

作     者：Madhusmita Panda Asmita Sandeep Kumar Purusottam Mishra Mahesh Chandra Sahu Sachinandan De Tirtha Kumar Datta Rakesh Kumar 

作者机构：Nodal OfficerChief District Veterinary OfficeKadambedaBhadrak756100OdishaIndia Institute of Microbial TechnologyCouncil of Scientific and Industrial ResearchChandigarhIndia BSL-4National Institute of VirologySus RoadPashanPune-411021India Goat Breeding FarmAT/PO-SalapadaDistrict-KeonjharPin code-758020State-OdishaIndia Directorate of Medical ResearchIMS and SUM HospitalSiksha'O'Anusandhan UniversityK8Kalinga NagarBhubaneswar-751003OdishaIndia Animal Biotechnology CentreNational Dairy Research InstituteKarnal132001HaryanaIndia 

出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)

年 卷 期：2018年第7卷第2期

页      面：79-86页

学科分类：0710[理学-生物学] 1002[医学-临床医学] 0905[农学-畜牧学] 0906[农学-兽医学] 0901[农学-作物学] 100214[医学-肿瘤学] 0902[农学-园艺学] 10[医学] 

基　　金：National Fund, (NF2049/3040) National Fund Project of Indian Council of Agricultural Research Indian Council of Agricultural Research, ICAR 

主　　题：BCB screening Progesterone Sperm cumulus interaction Fertilizing ability 

摘      要：Objective:To understand the level of progesterone (P4) in different quality of buffalo cumulus oocyte complexes (COCs) and further to evaluate the effect of exogenous P4 supplementation on maturation and subsequent developmental ability of poor quality brilliant cresyl blue (BCB-)***: Progesterone secreted by different quality of buffalo oocytes was estimated by enzyme linked immunosorbent assay and the concentration differences were translated into P4 doses to be incorporated in the maturation medium of BCB-ve COCs followed by expression analysis of genes involved in the cumulus expansion, extracellular matrix disintegration and progesterone receptor signalling. In addition, the study also evaluated the effect of exogenous P4 on sperm-cumulus interaction. Results: More than 10-fold up-regulated expression of progesterone receptor in P4 supplemented oocytes signified that P4 might be acting predominantly through this receptor. Also, exogenous P4 supplementation had significant effect on transcatheter arterial chemoembolization protease regulated by P4-progesterone receptor pathway which in turn had an important role in extracellular matrix disintegration. On the contrary, cumulus expansion genesHAS2,TNFAIP6,AREG were not altered upon P4 supplementation. Also, it was observed that P4 addition did facilitate passage of significantly more number of spermatozoa through P4 treated cumulus cells. Further, incorporation of different doses of P4 did not improve significantly the cleavage and blastocyst rates of BCB-ve ***: Different qualities of buffalo COCs secrete substantially diverse levels of P4, and its supplementation has a role in oocyte maturation via modulation of cumulus characteristics but perhaps not fertilization.