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Harnessing the synergy of perfusable muscle flap matrix and adipose-derived stem cells for prevascularization and macrophage polarization to reconstruct volumetric muscle loss

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Bioactive Materials 2023年第4期22卷 588-614页

作者： Qixu Zhang Yulun Chiu Youbai Chen Yewen Wu Lina W.dunne rene d.Largo Edward I.Chang david M.Adelman Mark V.Schaverien Charles E.Butler department of Plastic Surgery The University of Texas MD Anderson Cancer CenterHoustonTX77030USA department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer CenterHoustonTX77030USA department of Plastic Surgery Chinese PLA General HospitalBeijing100853China

Muscle flaps must have a strong vascular network to support a large tissue volume and ensure successful *** developed porcine stomach musculofascial flap matrix(PdSF)comprising extracellular matrix(ECM)and intact *** had a dominant vascular pedicle,microcirculatory vessels,a nerve network,well-retained 3-dimensional(3d)nanofibrous ECM structures,and no allo-or ***-depth proteomic analysis demonstrated that PdSF was composed of core matrisome proteins(e.g.,collagens,glycoproteins,proteoglycans,and ECM regulators)that,as shown by Gene Ontology term enrichment analysis,are functionally related to musculofascial biological ***,PdSF􀀀human adipose-derived stem cell(hASC)synergy not only induced monocytes towards IL-10􀀀producing M2 macrophage polarization through the enhancement of hASCs’paracrine effect but also promoted the proliferation and interconnection of both human skeletal muscle myoblasts(HSMMs)and human umbilical vein endothelial cells(HUVECs)in static triculture ***,PdSF was successfully prevascularized through a dynamic perfusion coculture of hASCs and HUVECs,which integrated with PdSF and induced the maturation of vascular networks in *** a xenotransplantation model,PdSF demonstrated myoconductive and immunomodulatory properties associated with the predominance of M2 macrophages and regulatory T *** a volumetric muscle loss(VML)model,prevascularized PdSF augmented neovascularization and constructive remodeling,which was characterized by the predominant infiltration of M2 macrophages and significant musculofascial tissue *** results indicate that hASCs’integration with PdSF enhances the cells’dual function in immunomodulation and *** in part to this PdSF-hASC synergy,our platform shows promise for vascularized muscle flap engineering for VML reconstruction.

关键词： Muscle flap fabrication Extracellular matrix decellularization Vascularization Macrophage polarization Volumetric muscle loss

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MPFracNet:A deep Learning Algorithm for Metacarpophalangeal Fracture detection with Varied difficulties

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Computers, Materials & Continua 2023年第4期75卷 999-1015页

作者： Geng Qin Ping Luo Kaiyuan Li Yufeng Sun Shiwei Wang Xiaoting Li Shuang Liu Linyan Xue College of Quality and Technical Supervision Hebei UniversityBaoding071002China Hebei Technology Innovation center for Lightweight of New Energy Vehicle Power System Baoding071002China National&Local Joint Engineering research center of Metrology Instrument and System Hebei UniversityBaoding071002China

due to small size and high occult,metacarpophalangeal fracturediagnosis displays a low accuracy in terms of fracture detection and locationin X-ray *** efficiently detect metacarpophalangeal fractures on Xrayimages as the second opinion for radiologists,we proposed a novel onestageneural network namedMPFracNet based ***,a deformable bottleneck block(dBB)was integrated into the bottleneckto better adapt to the geometric variation of the ***,an integrated feature fusion module(IFFM)was employed to obtain morein-depth semantic and shallow detail ***,Focal Loss andBalanced L1 Loss were introduced to respectively attenuate the imbalancebetween positive and negative classes and the imbalance between detectionand location *** assessed the proposed model on the test set andachieved an AP of 80.4%for the metacarpophalangeal fracture *** estimate the detection performance for fractures with different difficulties,the proposed model was tested on the subsets of metacarpal,phalangeal andtiny fracture test sets and achieved APs of 82.7%,78.5%and 74.9%,*** proposed framework has state-of-the-art performance for detectingmetacarpophalangeal fractures,which has a strong potential application valuein practical clinical environments.

关键词： deep learning small object detection metacarpophalangeal fractures computer-aided diagnosis(CAd)

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Comparison of short-term toxicity of 14 common phycotoxins(alone and in combination)to the survival of brine shrimp Artemia salina

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Acta Oceanologica Sinica 2023年第2期42卷 134-141页

作者： Yuting Zhang Shanshan Song Bin Zhang Yang Zhang Miao Tian Ziyi Wu Huorong Chen Guangmao ding renyan Liu Jingli Mu Institute of Oceanography College of Geography and OceanographyMinjiang UniversityFuzhou350108China Fuzhou Institute of Oceanography Fuzhou350108China department of Marine Chemistry National Marine Environmental Monitoring CenterDalian116023China Fujian Marine Environment and Fishery resource Monitoring center Fuzhou350003China

Toxic harmful algal blooms(HABs)can cause deleterious effects in marine organisms,threatening the stability of marine *** is well known that different strains,natural populations and growth conditions of the same toxic algal species may lead to different amount of phycotoxin production and the ensuing *** fully assess the ecological risk of toxic HABs,it is of great importance to investigate the toxic effects of phycotoxins in marine *** this study,the short-term toxicity of 14 common phycotoxins(alone and in combination)in the marine zooplankton Artemia salina was *** 48 h LC_(50)of the 14 phycotoxins varied from 0.0193µg/mL to 2.415µg/*** most potent phycotoxin was azaspiracids-3(AZA3;with a LC_(50)of 0.0193µg/mL),followed by azaspiracids-2(AZA2;0.0226µg/mL),pectenotoxin-2(PTX2;0.0460µg/mL)and dinophysistoxin-1(dTX1;0.0818µg/mL).For the binary exposure,okadaic acid(OA)induced potential additive effects with dTX1,probably due to their similar structure(polyether fatty acid)and mode of action(attacking the serine/threonine phosphoprotein phosphatases).On the other hand,OA showed potential antagonistic effects with PTX2,which might be accounted for by their activation on the detoxification activity of cytochrome P450 *** addition,dTX1 induced potential synergetic effects with saxitoxin(STX),yessotoxin(YTX)or PTX2,suggesting the hazard potency of the mixtures of dTX1 and other phycotoxins(like STX,YTX and PTX2)with regard to the ecological *** results provide valuable toxicological data for assessing the impact of phycotoxins on marine planktonic species and highlight the potential ecological risk of toxic HABs in marine ecosystems.

关键词： LC_(50) harmful algal blooms binary exposure ecological risk

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Chemical Synthesis of Proteins Containing 300 Amino Acids

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Chemical research in Chinese Universities 2020年第5期36卷 733-747页

作者： ZHANG Baochang LI Yulei SHI Weiwei WANG Tongyue ZHANG Feng LIU Lei Tsinghua-Peking center for Life Sciences Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education)Center for Synthetic and Systems BiologyState Key Laboratory of Chemical Oncogenomics(Shenzhen)Department of ChemistryTsinghua UniversityBeijing 100084P.R.China

Chemical synthesis of proteins containing up to 300 amino acids may cover 30%—50%of all the proteins encountered in biomedical studies and may provide an alternate approach to the usually used recombinant expression teclmology,vastly expanding the chemical space of the *** the present review article,we tried to survey the recent progresses made for more rapid synthesis of increasingly long peptides and more efficient ligation of multiple peptide *** developments of seminal methods by many research groups have greatly contributed to the recent breakthroughs in the successful total synthesis of a number of functionally important proteins,such as oligoubiquitins,bacterial GroEL/ES chaperones,and mirror-image dNA *** these studies,a potential bottleneck has also been recognized for the chemical synthesis of large proteins,namely,how to ensure that each peptide segment from a large protein avoids unfavorable aggregation when dissolved in aqueous *** new methods,such as removable backbone modification(rBM)strategy have been developed to overcome this bottleneck,while more studies need to be carried out to develop more effective and less costly methods that ultimately,may lead to fully automatable chemical synthesis of customized proteins of 300 amino acids bearing any artificial designs.

关键词： Chemical protein synthesis Solid-phase synthesis Peptide Ligation 300 amino acid

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Single-cell transcriptomics of immune cells in lymph nodes reveals their composition and alterations in functional dynamics during the early stages of bubonic plague

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Science China(Life Sciences) 2023年第1期66卷 110-126页

作者： Yifan Zhao Tong Wang Ziyang Liu Yuehua Ke ruoyan Li Hongyan Chen Yang You Gengshan Wu Shiyang Cao Zongmin du Fan Bai ruifu Yang Biomedical Pioneering Innovation center(BIOPIC) School of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China center for disease Control and Prevention of Chinese People’s Liberation Army Beijing 100071China

Bubonic plague caused by Yersinia pestis is highly infectious and often *** of the host immune response and its subsequent suppression by *** is critical to understanding the pathogenesis of ***,we utilized single-cell rNA sequencing to systematically profile the transcriptomes of immune cells in draining lymph nodes(d LNs)during the early stage of *** *** cells responded to *** within 2 h post-infection(hpi),followed by the activation of macrophages/monocytes(Mφs/Mons)and recruitment of polymorphonuclear neutrophils(PMNs)to d LNs at 24 *** of cell-to-cell communication suggests that PMNs may be recruited to lymph nodes following the secretion of CCL9 by Mφs/Mons stimulated through CCr1-CCL9 *** functional suppression of all the three innate immune cell types occurred during the early stage of *** summary,we present a dynamic immune landscape,at single-cell resolution,of murine d LNs involved in the response to *** infection,which may facilitate the understanding of the plague pathogenesis of during the early stage of infection.

关键词： bubonic plague single-cell rNA sequencing Yersinia pestis

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Inhibition of Cdk5 increases osteoblast differentiation and bone mass and improves fracture healing

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Bone research 2022年第3期10卷 469-481页

作者： Mubashir Ahmad Benjamin Thilo Krüger Torsten Kroll Sabine Vettorazzi Ann-Kristin dorn Florian Mengele Sooyeon Lee Sayantan Nandi dilay Yilmaz Miriam Stolz Naveen Kumar Tangudu david Carro Vázquez Johanna Pachmayr Ion Cristian Cirstea Maja Vujic Spasic Aspasia Ploubidou Anita Ignatius Jan Tuckermann Institute of Comparative Molecular Endocrinology(CME) Ulm UniversityHelmholtzstrasse 8/189081 UlmGermany Institute of Orthopedic research and Biomechanics Ulm UniversityHelmholtzstrasse 1489081 UlmGermany Leibniz Institute on Aging–Fritz Lipmann Institute(FLI) Beutenbergstrasse 11D-07745 JenaGermany Praxisklinik für Orthopädie Unfall-und Neurochirurgie Prof.Bischoff/Dr.Spies/Dr.Mengele89231 Neu-UlmGermany Paracelsus Medizinische Privatuniverstät Institute of PharmacyStrubergasse 215020 SalzburgAustria department of Endocrinology Ludwig Maximilians University MunichMunich 80336Germany Present address:Institute of Orthopedic research and Biomechanics Ulm UniversityHelmholtzstrasse 1489081 UlmGermany Present address:UPMC Hillman Cancer center Department of Pharmacology and Chemical BiologyUniversity of Pittsburgh5115 Center Avenue15232 PittsburghPAUSA Present address:TAmirNA GmbH Leberstrasse 201110 ViennaAustria

Identification of regulators of osteoblastogenesis that can be pharmacologically targeted is a major goal in combating osteoporosis,a common disease of the elderly population. Here, unbiased kinome rNAi screening in primary murine osteoblasts identified cyclin-dependent kinase 5(Cdk5) as a suppressor of osteoblast differentiation in both murine and human preosteoblastic cells. Cdk5 knockdown by si rNA, genetic deletion using the Cre-lox P system, or inhibition with the small molecule roscovitine enhanced osteoblastogenesis in vitro. roscovitine treatment significantly enhanced bone mass by increasing osteoblastogenesis and improved fracture healing in mice. Mechanistically, downregulation of Cdk5 expression increased Erk phosphorylation, resulting in enhanced osteoblast-specific gene expression. Notably, simultaneous Cdk5 and Erk depletion abrogated the osteoblastogenesis conferred by Cdk5 depletion alone, suggesting that Cdk5 regulates osteoblast differentiation through MAPK pathway modulation. We conclude that Cdk5 is a potential therapeutic target to treat osteoporosis and improve fracture healing.

关键词： Cdk5 healing expression

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Top-dressing nitrogen fertilizer rate contributes to decrease culm physical strength by reducing structural carbohydrate content in japonica rice

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Journal of Integrative Agriculture 2016年第5期15卷 992-1004页

作者： ZHANG Wu-jun WU Long-mei dING Yan-feng WENG Fei WU Xiao-ran LI Gang-hua LIU Zheng-hui TANG She dING Cheng-qiang WANG Shao-hua Jiangsu Collaborative Innovation center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China Nanjing Agricultural University Nanjing 210095 P.R. China

Lodging is an important factor limiting rice yield and quality by bending or breaking stem in japonica rice（Oryza sativa L.） production. The objectives of this study were to determine the mechanism of lodging resistance in japonica rice as affected by carbohydrate components, especially its related arrangement in culm tissue and response to top-dressing nitrogen（N） fertilizer. Field experiments were conducted in danyang County, Jiangsu Province, China, by using two japonica rice varieties Wuyunjing 23（lodging-resistance variety） and W3668（lodging-susceptible variety） with three top-dressing N fertilizer rates（0, 135 and 270 kg N ha^（-1）） in 2013 and 2014. Lodging related physical parameters, morphological characteristics and stem carbohydrate components were investigated at 30 d after full heading stage. results showed that with increasing N fertilizer rates, the lodging rate and lodging index increased rapidly primarily due to significant reduction of breaking strength in two japonica rice varieties. Correlation analysis revealed that breaking strength was significantly and positively correlated with bending stress, but negatively correlated with section modulus, except for significant correlation at W3668 in 2014. Higher stem plumpness status and structural carbohydrate contents significantly enhanced stem stiffness, despite of lower non-structural carbohydrate. With higher N fertilizer rate, the culm wall thickness was almost identical, and culm diameter increased slightly. The structural carbohydrates, especially for lignin content in culm, reduced significantly under high N rate. Further histochemical staining analysis revealed that high N treatments decreased the lignin deposition rapidly in the sclerenchyma cells of mechanical tissue, large vascular bundle and small vascular bundle region, which were consistent with reduction of bending stress, especially for W3668 and thus, resulted in poor stem strength and higher lodging index. These results suggested that

关键词： japonica rice lodging resistance nitrogen stem strength structural carbohydrate

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recent advances in ultrathin materials and their applications in e-skin

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InfoMat 2023年第8期5卷 1-36页

作者： Wenchao Gao Jiaoya Huang Jiang He runhui Zhou Zemin Li Ziyu Chen Yufei Zhang Caofeng Pan CAS center for Excellence in Nanoscience Beijing Key Laboratory of Micro-Nano Energy and SensorBeijing Institute of Nanoenergy and NanosystemsChinese Academy of SciencesBeijingthe People's Republic of China center on Nanoenergy research School of Physical Science and TechnologyGuangxi UniversityNanningthe People's Republic of China

Intelligent technologies based on artificial intelligence and big data hold great potential for health monitoring and human–machine capability ***,electronics must be connected to the human body to realize this ***,tissue or skin-like electronics with high stretchability and low stiffness mechanical properties are highly *** materials have attracted significant attention from the research community and the industry because of their high performance and *** the past few years,considerable progress has been made in flexible ultrathin sensors and devices based on ultrathin ***,we review the developments in this area and examine representative research progress in ultrathin materials fabrication and device *** for the fabrication of stretchable ultrathin materials and devices are *** relationship between the thin-film structure and performance is emphasized and ***,the current capabilities and limitations of ultrathin devices were explored.

关键词： e-skin flexible electronics ultrathin devices ultrathin materials wearable devices

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Ultrafast growth of high-quality large-sized GaSe crystals by liquid metal promoter

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Nano research 2022年第5期15卷 4677-4681页

作者： Zuxin Chen Quan Chen Zebing Chai Bin Wei Jun Wang Yanping Liu Yumeng Shi Zhongchang Wang Jingbo Li Institute of semiconductors South China Normal UniversityGuangzhou 510631China School of Materials Sun Yat-Sen UniversityGuangzhou 510275China School of Electrical Engineering and Automation Wuhan UniversityWuhan 430000China School of Physics and Electronics Hunan Key Laboratory for Super-microstructure and Ultrafast ProcessCentral South University932 South Lushan RoadChangsha 410083China Engineering Technology research center for 2d Material Information Function devices and Systems of Guangdong Province International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationInstitute of Microscale OptoelectronicsShenzhen UniversityShenzhen 518060China International Iberian Nanotechnology Laboratory(INL) 4715–330 BragaPortugal

Growth of high-quality large-sized crystals using the traditional chemical vapor transport(CVT)or vertical Bridgman(VB)technique is costly and time-consuming,limiting its practical industrial ***,we propose an ultrafast crystal growth process with low energy consumption and capability of producing crystals of excellent quality,and demonstrate that large-sized GaSe crystals with a lateral size of 0.5 to 1 cm can be obtained within a short period of 5 min.X-ray diffraction(Xrd)and scanning transmission electron microscopy(STEM)studies clearly indicate that the as-grown crystals have a good *** further show the potential application of the resulting GaSe crystals,we fabricate the few-layer GaSe-based photodetector,which exhibits low dark current of 21 pA and fast response of 34 ms under 405 nm *** proposed technique for rapid crystal growth could be further extended to other metallenes with low-melting point,such as Bi-,Sn-,In-,Pb-based crystals,opening up a new avenue in fulfilling diverse potential optoelectronics applications of two-dimensional(2d)crystals.

关键词： GaSe ultrafast growth photodetector liquid metal

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Charactering protein fraction concentrations as influenced by nitrogen application in low-glutelin rice cultivars

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Journal of Integrative Agriculture 2016年第3期15卷 537-544页

作者： LI Gang-hua CHEN Yi-lu dING Yan-feng GENG Chun-miao LI Quan LIU Zheng-hui WANG Shao-hua TANG She Jiangsu Collaborative Innovation center for Modern Crop Production/Jiangsu Key Laboratory for Information Agriculture/National Engineering and Technology center for Information Agriculture/Key Laboratory of Crop Physiology and Ecology in Southern China Nanjing Agricultural University Nanjing 210095 P.R.China

To optimize both grain yield and quality of low-glutelin rice cultivars under N-fertilizer strategies, two-year field experiments involving three low-glutelin rice cultivars（W1240, W1721, W025） and an ordinary rice cultivar（H9405） with five N treatments were carried out to determine the effects of N application rate and genotype on protein fractions contents and Glutelin/Prolamin ratio（Glu/Pro）. The difference of protein fraction concentrations affected by N application rate existed in genotypes. Ordinary rice cultivar had a larger increase in glutlein concentration affected by N application rate than low-glutelin rice cultivars did. Glutelin in H9405 had a increase of 30.6 and 41.0% under the N4 treatment（360 kg N ha^（–1）） when compared with N0 treatment（no fertilizer N） in 2010 and 2011 respectively, while all the low-glutelin rice cultivars showed relatively smaller increases for two years. Variance analysis showed no significant effect of N application rate on glutelin in W1240 and W025 while the effects on albumin, globulin and prolamin were significant in low-glutelin rice. What＇s more, N treatment had no significant i nfluence on Glu/Pro ratios in low-glutelin rice cultivars while a significant increase in Glu/Pro ratio was observed in ordinary rice cultivar. So low-gultelin rice cultivars showed a different pattern from ordinary rice cultivars when influenced by N application rate.

关键词： low-glutelin rice nitrogen protein fractions Glu/Pro

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